Article ; Online: Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study.
2020 Volume 27, Issue 2, Page(s) 285.e1–285.e4
Abstract: ... the potential use of saliva samples as a non-invasive tool for the diagnosis of COVID-19.: Methods: From 27 ... there is a need for a quick and easy method to obtain a non-invasive sample for the detection ... Objectives: Amid the increasing number of pandemic coronavirus disease 2019 (COVID-19) cases ...
| Abstract | Objectives: Amid the increasing number of pandemic coronavirus disease 2019 (COVID-19) cases, there is a need for a quick and easy method to obtain a non-invasive sample for the detection of this novel coronavirus (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2). We aimed to investigate the potential use of saliva samples as a non-invasive tool for the diagnosis of COVID-19. Methods: From 27 March to 4 April 2020, we prospectively collected saliva samples and a standard nasopharyngeal and throat swab in persons seeking care at an acute respiratory infection clinic in a university hospital during the outbreak of COVID-19. Real-time polymerase chain reaction (RT-PCR) was performed, and the results of the two specimens were compared. Results: Two-hundred pairs of samples were collected. Sixty-nine (34.5%) individuals were male, and the median (interquartile) age was 36 (28-48) years. Using nasopharyngeal and throat swab RT-PCR as the reference standard, the prevalence of COVID-19 diagnosed by nasopharyngeal and throat swab RT-PCR was 9.5%. The sensitivity and specificity of the saliva sample RT-PCR were 84.2% (95% CI 60.4%-96.6%), and 98.9% (95% CI 96.1%-99.9%), respectively. An analysis of the agreement between the two specimens demonstrated 97.5% observed agreement (κ coefficient 0.851, 95% CI 0.723-0.979; p < 0.001). Conclusions: Saliva might be an alternative specimen for the diagnosis of COVID-19. The collection is non-invasive, and non-aerosol generating. This method could facilitate the diagnosis of the disease, given the simplicity of specimen collection and good diagnostic performance. |
|---|---|
| MeSH term(s) | Adult ; COVID-19/diagnosis ; COVID-19 Testing/methods ; Cross-Sectional Studies ; Female ; Humans ; Male ; Middle Aged ; Nasopharynx/virology ; Prospective Studies ; RNA, Viral/analysis ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2/genetics ; Saliva/virology ; Sensitivity and Specificity ; Specimen Handling/methods ; Viral Load/methods |
| Chemical Substances | RNA, Viral |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-05-15 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 1328418-6 |
| ISSN | 1469-0691 ; 1470-9465 ; 1198-743X |
| ISSN (online) | 1469-0691 |
| ISSN | 1470-9465 ; 1198-743X |
| DOI | 10.1016/j.cmi.2020.05.001 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 4541: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
|||
| Zs.MO 284: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.