Article ; Online: Enhanced Cholesterol-Dependent Hemifusion by Internal Fusion Peptide 1 of SARS Coronavirus-2 Compared to Its N-Terminal Counterpart.
2021 Volume 60, Issue 8, Page(s) 559–562
Abstract: ... IFP1) of SARS-CoV-2 is far more efficient than its N-terminal counterpart (FP) to induce hemifusion ... coronaviruses contain more than one fusion peptide sequences. We have shown that the internal fusion peptide 1 ... instrumental in fusion and is called as a "fusion peptide". However, severe acute respiratory syndrome (SARS ...
| Abstract | Membrane fusion is an important step for the entry of the lipid-sheathed viruses into the host cells. The fusion process is being carried out by fusion proteins present in the viral envelope. The class I virus contains a 20-25 amino acid sequence at its N-terminal of the fusion domain, which is instrumental in fusion and is called as a "fusion peptide". However, severe acute respiratory syndrome (SARS) coronaviruses contain more than one fusion peptide sequences. We have shown that the internal fusion peptide 1 (IFP1) of SARS-CoV-2 is far more efficient than its N-terminal counterpart (FP) to induce hemifusion between small unilamellar vesicles. Moreover, the ability of IFP1 to induce hemifusion formation increases dramatically with growing cholesterol content in the membrane. Interestingly, IFP1 is capable of inducing hemifusion but fails to open the pore. |
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| MeSH term(s) | Amino Acid Sequence ; COVID-19/genetics ; COVID-19/metabolism ; Cholesterol/genetics ; Cholesterol/metabolism ; Humans ; Membrane Fusion/physiology ; Peptide Fragments/genetics ; Peptide Fragments/metabolism ; Phosphatidylcholines/genetics ; Phosphatidylcholines/metabolism ; SARS-CoV-2/genetics ; SARS-CoV-2/metabolism ; Virus Internalization |
| Chemical Substances | Peptide Fragments ; Phosphatidylcholines ; Cholesterol (97C5T2UQ7J) ; 1,2-oleoylphosphatidylcholine (EDS2L3ODLV) |
| Language | English |
| Publishing date | 2021-02-11 |
| Publishing country | United States |
| Document type | Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1108-3 |
| ISSN | 1520-4995 ; 0006-2960 |
| ISSN (online) | 1520-4995 |
| ISSN | 0006-2960 |
| DOI | 10.1021/acs.biochem.1c00046 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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