Article ; Online: EWI-2wint promotes CD81 clustering that abrogates Hepatitis C Virus entry.
2013 Volume 15, Issue 7, Page(s) 1234–1252
Abstract: ... an increase in the proportion of confined molecules. Indeed, we demonstrated that EWI-2wint promotes CD81 ... CD81 is a major receptor for Hepatitis C Virus (HCV). It belongs to the tetraspanin ... clustering and confinement in CD81-enriched areas. In addition, we showed that EWI-2wint influences ...
| Abstract | CD81 is a major receptor for Hepatitis C Virus (HCV). It belongs to the tetraspanin family whose members form dynamic clusters with numerous partner proteins and with one another, forming tetraspanin-enriched areas in the plasma membrane. In our study, we combined single-molecule microscopy and biochemistry experiments to investigate the clustering and membrane behaviour of CD81 in the context of cells expressing EWI-2wint, a natural inhibitor of HCV entry. Interestingly, we found that EWI-2wint reduces the global diffusion of CD81 molecules due to a decrease of the diffusion rate of mobile CD81 molecules and an increase in the proportion of confined molecules. Indeed, we demonstrated that EWI-2wint promotes CD81 clustering and confinement in CD81-enriched areas. In addition, we showed that EWI-2wint influences the colocalization of CD81 with Claudin-1 - a co-receptor required for HCV entry. Together, our results indicate that a change in membrane partitioning of CD81 occurs in the presence of EWI-2wint. This study gives new insights on the mechanism by which HCV enters into its target cells, namely by exploiting the dynamic properties of CD81. |
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| MeSH term(s) | Antigens, CD/metabolism ; Cell Line ; Hepacivirus/physiology ; Hepatocytes/virology ; Host-Pathogen Interactions ; Humans ; Membrane Proteins/metabolism ; Receptors, Virus/metabolism ; Tetraspanin 28/metabolism ; Virus Internalization |
| Chemical Substances | Antigens, CD ; IGSF8 protein, human ; Membrane Proteins ; Receptors, Virus ; Tetraspanin 28 |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2013-02-16 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1468320-9 |
| ISSN | 1462-5822 ; 1462-5814 |
| ISSN (online) | 1462-5822 |
| ISSN | 1462-5814 |
| DOI | 10.1111/cmi.12112 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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