Artikel: Vimentin-positive cells in the epithelium of rabbit ileal villi represent cup cells but not M-cells.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
2003 Band 51, Heft 11, Seite(n) 1533–1544
Abstract: ... We conclude (a) that the vimentin-positive cells in ordinary villi represent cup cells but not M-cells, (b ... identified in ordinary rabbit ileal villi, which are vimentin-positive and share morphological ... for N-acetyl-glucosamine oligomers selectively bound to the vimentin-positive villous cells but not to M ...
| Abstract | Membranous (M)-cells are specialized epithelial cells of the Peyer's patch domes that transport antigens from the intestinal lumen to the lymphoid tissue. Vimentin is a reliable marker for M-cells in rabbits. Using immunohistochemistry (IHC), a subpopulation of epithelial cells has recently been identified in ordinary rabbit ileal villi, which are vimentin-positive and share morphological characteristics with the M-cells of the domes. To test the hypothesis that these cells represent M-cells outside the organized lymphoid tissue, lectin labeling and tracer uptake experiments were performed. Lectins specific for N-acetyl-glucosamine oligomers selectively bound to the vimentin-positive villous cells but not to M-cells in the domes. Microbeads instilled into the ileal lumen were taken up by M-cells within 45 min but not by the vimentin-positive cells in the villi. Lectin-gold labeling on ultrathin sections revealed that the lectin binding sites were located in the brush border and in vesicles in the apical cytoplasm. The vimentin/lectin-positive cells shared ultrastructural characteristics with the so-called "cup cells." We conclude (a) that the vimentin-positive cells in ordinary villi represent cup cells but not M-cells, (b) that they are readily detectable by (GlucNAc)(N)-specific lectins, and (c) that they do not transcytose experimental tracers. Although the specific function of cup cells is still obscure, they most probably represent a cell type distinct from M-cells of the domes with respect to both function and expression of the two new markers. |
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| Mesh-Begriff(e) | Animals ; Ileum/cytology ; Ileum/metabolism ; Ileum/ultrastructure ; Immunohistochemistry ; Intestinal Mucosa/cytology ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/ultrastructure ; Lectins/metabolism ; Microscopy, Confocal ; Microscopy, Fluorescence ; Microvilli/metabolism ; Microvilli/ultrastructure ; Rabbits ; Vimentin/metabolism | |||||
| Chemische Substanzen | Lectins ; Vimentin | |||||
| Sprache | Englisch | |||||
| Erscheinungsdatum | 2003-11 | |||||
| Erscheinungsland | United States | |||||
| Dokumenttyp | Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 218208-7 | |||||
| ISSN | 1551-5044 ; 0022-1554 | |||||
| ISSN (online) | 1551-5044 | |||||
| ISSN | 0022-1554 | |||||
| DOI | 10.1177/002215540305101113 | |||||
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| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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