Article: Involvement of the mannose receptor in infection of macrophages by influenza virus.
2000 Volume 74, Issue 11, Page(s) 5190–5197
Abstract: ... with the infectivity of each virus for macrophages. Infection of macrophages, but not MDCK cells, by influenza virus ... of influenza virus, and perhaps other enveloped viruses, into murine macrophages. ... of macrophages by influenza virus. In competitive binding studies, the binding of (125)I-labeled mannosylated ...
| Abstract | Influenza viruses A/PR/8/34 (PR8; H1N1), A/Aichi/68 X-31 (HKx31; H3N2), and A/Beijing/89 X-109 (BJx109; H3N2) show marked differences in their ability to infect murine macrophages, including resident alveolar and peritoneal macrophages as well as the macrophage-derived cell line J774. The hierarchy in infectivity of the viruses (PR8 < HKx31 < BJx109) resembles that of their reactivity with mannose-binding lectins of the collectin family. Since the macrophage mannose receptor recognizes the same spectrum of monosaccharides as the collectins do, we investigated the possible involvement of this receptor in infection of macrophages by influenza virus. In competitive binding studies, the binding of (125)I-labeled mannosylated bovine serum albumin to macrophages was inhibited by the purified hemagglutinin and neuraminidase (HANA) glycoproteins of influenza virus but not by HANA that had been treated with periodate to oxidize its oligosaccharide side chains. The inhibitory activity of HANA from the three strains of virus differed markedly and correlated with the infectivity of each virus for macrophages. Infection of macrophages, but not MDCK cells, by influenza virus was inhibited by yeast mannan. A variant line of J774 cells, J774E, which expresses elevated levels of the mannose receptor, was more readily infected than J774, and the sensitivity of J774E cells to infection was greatly reduced by culture in the presence of D-mannose, which down-modulated mannose receptor expression. Together, the data implicate the mannose receptor as a major endocytic receptor in the infectious entry of influenza virus, and perhaps other enveloped viruses, into murine macrophages. |
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| MeSH term(s) | Animals ; Cell Line ; Cells, Cultured ; Dogs ; Hemagglutinin Glycoproteins, Influenza Virus/metabolism ; Humans ; Influenza A virus/metabolism ; Influenza A virus/physiology ; Lectins, C-Type ; Macrophages, Alveolar/cytology ; Macrophages, Alveolar/metabolism ; Macrophages, Alveolar/virology ; Macrophages, Peritoneal/cytology ; Macrophages, Peritoneal/metabolism ; Macrophages, Peritoneal/virology ; Mannans/metabolism ; Mannose-Binding Lectins ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; N-Acetylneuraminic Acid/metabolism ; Neuraminidase/metabolism ; Receptors, Cell Surface/metabolism | |||||
| Chemical Substances | Hemagglutinin Glycoproteins, Influenza Virus ; Lectins, C-Type ; Mannans ; Mannose-Binding Lectins ; Receptors, Cell Surface ; mannose receptor ; Neuraminidase (EC 3.2.1.18) ; N-Acetylneuraminic Acid (GZP2782OP0) | |||||
| Language | English | |||||
| Publishing date | 2000-06 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 80174-4 | |||||
| ISSN | 1098-5514 ; 0022-538X | |||||
| ISSN (online) | 1098-5514 | |||||
| ISSN | 0022-538X | |||||
| DOI | 10.1128/jvi.74.11.5190-5197.2000 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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