LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 2 of total 2

Search options

  1. Article: P2X7 on Mouse T Cells: One Channel, Many Functions.

    Rissiek, Björn / Haag, Friedrich / Boyer, Olivier / Koch-Nolte, Friedrich / Adriouch, Sahil

    Frontiers in immunology

    2015  Volume 6, Page(s) 204

    Abstract: ... activation on mouse T cells and its versatile role in T cell homeostasis and activation. Further ... we will discuss important differences in the function of P2X7 on human and murine T cells. ... also an important regulatory receptor of murine and human T cell functions. Murine T cells express ...

    Abstract The P2X7 receptor is an adenosine triphosphate (ATP)-gated cation channel that is expressed by several cells of the immune system. P2X7 is best known for its proinflammatory role in promoting inflammasome formation and release of mature interleukin (IL)-1β by innate immune cells. Mounting evidence indicates that P2X7 is also an important regulatory receptor of murine and human T cell functions. Murine T cells express a sensitive splice variant of P2X7 that can be activated either by non-covalent binding of ATP or, in the presence of nicotinamide adenine dinucleotide, by its covalent ADP-ribosylation catalyzed by the ecto-ADP-ribosyltransferase ARTC2.2. Prolonged activation of P2X7 by either one of these pathways triggers the induction of T cell death. Conversely, lower concentrations of ATP can activate P2X7 to enhance T cell proliferation and production of IL-2. In this review, we will highlight the molecular and cellular consequences of P2X7 activation on mouse T cells and its versatile role in T cell homeostasis and activation. Further, we will discuss important differences in the function of P2X7 on human and murine T cells.
    Language English
    Publishing date 2015
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2015.00204
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Reanalysis of P2X7 receptor expression in rodent brain.

    Sim, Joan A / Young, Mark T / Sung, Hye-Youn / North, R Alan / Surprenant, Annmarie

    The Journal of neuroscience : the official journal of the Society for Neuroscience

    2004  Volume 24, Issue 28, Page(s) 6307–6314

    Abstract: ... we therefore studied mice for which there are two P2X7 knock-out mice constructs available, one expressing the LacZ ... in immune cells, where they act not only as cationic channels but uniquely couple with rapid release ... of proinflammatory cytokines, cytoskeletal rearrangements, and apoptosis or necrotic cell death. The P2X7 receptor ...

    Abstract P2X receptors are cationic-selective ion channels gated by extracellular ATP. There are seven subunits (P2X1-7), the first six of which are expressed throughout the peripheral and central nervous systems. P2X7 receptors are rapidly upregulated and activated as a result of inflammatory stimuli in immune cells, where they act not only as cationic channels but uniquely couple with rapid release of proinflammatory cytokines, cytoskeletal rearrangements, and apoptosis or necrotic cell death. The P2X7 receptor has been termed the cytolytic non-neuronal P2X receptor because it had not been detected in neurons until recently when it has been immunolocalized to several brain regions, particularly the hippocampus, and has been suggested to be involved in presynaptic modulation of transmitter release. Because its expression in brain neurons may have substantial functional implications, we have performed detailed immunocytochemical, immunoblot, and immunoprecipitation studies on brain and non-neuronal tissue using all currently available antibodies. We first examined rats, but staining patterns were inconsistent among antibodies; we therefore studied mice for which there are two P2X7 knock-out mice constructs available, one expressing the LacZ transgene. We found that P2X7 receptor protein is strongly and reliably detected in the submandibular gland and lung of wild-type mice but not in either of the P2X7-/- mice. However, we failed to find evidence for P2X7 receptor protein in hippocampal neurons or their input-output projections. Either the P2X7 protein in the hippocampus is below the limits of detection by the currently available methods or it is not present.
    MeSH term(s) Animals ; Antibodies, Monoclonal/immunology ; Brain Chemistry ; Crosses, Genetic ; Epitopes/immunology ; Hippocampus/chemistry ; Immunoprecipitation ; Lac Operon ; Lung/chemistry ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Mice, Knockout ; Nerve Tissue Proteins/analysis ; Nerve Tissue Proteins/deficiency ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/immunology ; Neurons/chemistry ; Organ Specificity ; Rats ; Rats, Wistar ; Receptors, Purinergic P2/analysis ; Receptors, Purinergic P2/deficiency ; Receptors, Purinergic P2/genetics ; Receptors, Purinergic P2/immunology ; Receptors, Purinergic P2X7 ; Submandibular Gland/chemistry
    Chemical Substances Antibodies, Monoclonal ; Epitopes ; Nerve Tissue Proteins ; P2rx7 protein, mouse ; Receptors, Purinergic P2 ; Receptors, Purinergic P2X7
    Language English
    Publishing date 2004-07-07
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604637-x
    ISSN 1529-2401 ; 0270-6474
    ISSN (online) 1529-2401
    ISSN 0270-6474
    DOI 10.1523/JNEUROSCI.1469-04.2004
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1668: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top