Article: TRP-PLIK, a bifunctional protein with kinase and ion channel activities.
2001 Volume 291, Issue 5506, Page(s) 1043–1047
Abstract: We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long ... a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO ... transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and ...
| Abstract | We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing alpha-kinase domain was functional. Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function. |
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| MeSH term(s) | Adenosine Triphosphate/metabolism ; Amino Acid Motifs ; Amino Acid Sequence ; Animals ; CHO Cells ; Calcium/metabolism ; Catalytic Domain ; Cations/metabolism ; Cell Line ; Cricetinae ; DNA, Complementary ; Electric Conductivity ; Humans ; Ion Channels/chemistry ; Ion Channels/genetics ; Ion Channels/metabolism ; Membrane Proteins ; Mice ; Molecular Sequence Data ; Mutation ; Myelin Basic Protein/metabolism ; Patch-Clamp Techniques ; Phosphorylation ; Protein Kinases/chemistry ; Protein Kinases/genetics ; Protein Kinases/metabolism ; Protein-Serine-Threonine Kinases ; Rats ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/metabolism ; TRPM Cation Channels ; Transfection ; Two-Hybrid System Techniques ; Type C Phospholipases/metabolism | ||||||||||
| Chemical Substances | Cations ; DNA, Complementary ; Ion Channels ; Membrane Proteins ; Myelin Basic Protein ; Recombinant Fusion Proteins ; TRPM Cation Channels ; Adenosine Triphosphate (8L70Q75FXE) ; Protein Kinases (EC 2.7.-) ; Trpm7 protein, mouse (EC 2.7.1.-) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; TRPM7 protein, human (EC 2.7.11.1) ; Trpm7 protein, rat (EC 2.7.11.1) ; Type C Phospholipases (EC 3.1.4.-) ; Calcium (SY7Q814VUP) | ||||||||||
| Language | English | ||||||||||
| Publishing date | 2001-02-09 | ||||||||||
| Publishing country | United States | ||||||||||
| Document type | Journal Article | ||||||||||
| ZDB-ID | 128410-1 | ||||||||||
| ISSN | 1095-9203 ; 0036-8075 | ||||||||||
| ISSN (online) | 1095-9203 | ||||||||||
| ISSN | 0036-8075 | ||||||||||
| DOI | 10.1126/science.1058519 | ||||||||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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