Article ; Online: Quantitative Assessment of SARS-CoV-2 Virus in Nasopharyngeal Swabs Stored in Transport Medium by a Straightforward LC-MS/MS Assay Targeting Nucleocapsid, Membrane, and Spike Proteins.
2021 Volume 20, Issue 2, Page(s) 1434–1443
Abstract: ... results. Here, we have developed a simple mass spectrometry assay for SARS-CoV-2 in nasopharyngeal swab ... Alternative methods to RT-PCR for SARS-CoV-2 detection are investigated to provide complementary ... quantification of N, M, and S proteins was achieved by addition of isotope-labeled versions of best peptides ...
| Abstract | Alternative methods to RT-PCR for SARS-CoV-2 detection are investigated to provide complementary data on viral proteins, increase the number of tests performed, or identify false positive/negative results. Here, we have developed a simple mass spectrometry assay for SARS-CoV-2 in nasopharyngeal swab samples using common laboratory reagents. The method employs high sensitivity and selectivity targeted mass spectrometry detection, monitoring nine constitutive peptides representative of the three main viral proteins and a straightforward pellet digestion protocol for convenient routine applications. Absolute quantification of N, M, and S proteins was achieved by addition of isotope-labeled versions of best peptides. Limit of detection, recovery, precision, and linearity were thoroughly evaluated in four representative viral transport media (VTM) containing distinct total protein content. The protocol was sensitive in all swab media with limit of detection determined at 2 × 10 |
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| MeSH term(s) | COVID-19/diagnosis ; COVID-19/virology ; Chromatography, Liquid/methods ; Culture Media ; Humans ; Nasopharynx/virology ; Nucleocapsid/metabolism ; Proteomics/methods ; Reproducibility of Results ; SARS-CoV-2/metabolism ; SARS-CoV-2/physiology ; Sensitivity and Specificity ; Specimen Handling/instrumentation ; Specimen Handling/methods ; Spike Glycoprotein, Coronavirus/metabolism ; Tandem Mass Spectrometry/methods ; Viral Proteins/metabolism | |||||
| Chemical Substances | Culture Media ; Spike Glycoprotein, Coronavirus ; Viral Proteins | |||||
| Language | English | |||||
| Publishing date | 2021-01-26 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 2078618-9 | |||||
| ISSN | 1535-3907 ; 1535-3893 | |||||
| ISSN (online) | 1535-3907 | |||||
| ISSN | 1535-3893 | |||||
| DOI | 10.1021/acs.jproteome.0c00887 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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