Article ; Online: Ability of recombinant human catalase to suppress inflammation of the murine lung induced by influenza A.
2014 Volume 37, Issue 3, Page(s) 809–817
Abstract: ... We have previously investigated the therapeutic efficacy of recombinant human catalase (rhCAT) against ... intranasally with high pathogenicity (H1N1) influenza A virus followed by treatment with recombinant human ... generic pharmacological strategies that reduce both viral replication and inflammation of the lung ...
| Abstract | Influenza A virus pandemics and emerging antiviral resistance highlight the urgent need for novel generic pharmacological strategies that reduce both viral replication and inflammation of the lung. We have previously investigated the therapeutic efficacy of recombinant human catalase (rhCAT) against viral pneumonia in mice, but the protection mechanisms involved were not explored. In the present study, we have performed a more in-depth analysis covering survival, lung inflammation, immune cell responses, production of cytokines, and inflammation signaling pathways in mice. Male imprinting control region mice were infected intranasally with high pathogenicity (H1N1) influenza A virus followed by treatment with recombinant human catalase. The administration of rhCAT resulted in a significant reduction in inflammatory cell infiltration (e.g., macrophages and neutrophils), inflammatory cytokine levels (e.g., IL-2, IL-6, TNF-α, IFN-γ), the level of the intercellular adhesion molecule 1 chemokine and the mRNA levels of toll-like receptors TLR-4, TLR-7, and NF-κB, as well as partially maintaining the activity of the antioxidant enzymes system. These findings indicated that rhCAT might play a key protective role in viral pneumonia of mice via suppression of inflammatory immune responses. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Animals ; Catalase/genetics ; Catalase/pharmacology ; Cytokines/biosynthesis ; Cytokines/blood ; Cytokines/genetics ; Humans ; Inflammation/drug therapy ; Influenza A Virus, H1N1 Subtype/pathogenicity ; Lung/pathology ; Macrophage Activation/drug effects ; Macrophages/immunology ; Male ; Malondialdehyde/metabolism ; Mice ; Mice, Inbred ICR ; Neutrophil Infiltration/drug effects ; Neutrophils/immunology ; Orthomyxoviridae Infections/drug therapy ; Oxidative Stress/immunology ; Pneumonia, Viral/drug therapy ; RNA, Messenger/genetics ; Random Allocation ; Reactive Oxygen Species/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/pharmacology ; Signal Transduction/immunology | |||||
| Chemical Substances | Cytokines ; RNA, Messenger ; Reactive Oxygen Species ; Recombinant Proteins ; Malondialdehyde (4Y8F71G49Q) ; Catalase (EC 1.11.1.6) | |||||
| Keywords | covid19 | |||||
| Language | English | |||||
| Publishing date | 2014-01-03 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 434408-x | |||||
| ISSN | 1573-2576 ; 0360-3997 | |||||
| ISSN (online) | 1573-2576 | |||||
| ISSN | 0360-3997 | |||||
| DOI | 10.1007/s10753-013-9800-2 | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
Full text online
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 1401: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.