Article: Oral Microbiome Dysbiosis Is Associated With Symptoms Severity and Local Immune/Inflammatory Response in COVID-19 Patients: A Cross-Sectional Study.
2021 Volume 12, Page(s) 687513
Abstract: ... aimed to define the HOM in COVID-19 patients, to evidence any association between its profile and ... immunoglobulins A (sIgA). The results showed the presence of oral dysbiosis in COVID-19 patients compared ... 19 subjects. Notably, oral dysbiosis correlated with symptom severity ( ...
| Abstract | The human oral microbiome (HOM) is the second largest microbial community after the gut and can impact the onset and progression of several localized and systemic diseases, including those of viral origin, especially for viruses entering the body via the oropharynx. However, this important aspect has not been clarified for the new pandemic human coronavirus SARS-CoV-2, causing COVID-19 disease, despite it being one of the many respiratory viruses having the oropharynx as the primary site of replication. In particular, no data are available about the non-bacterial components of the HOM (fungi, viruses), which instead has been shown to be crucial for other diseases. Consistent with this, this study aimed to define the HOM in COVID-19 patients, to evidence any association between its profile and the clinical disease. Seventy-five oral rinse samples were analyzed by Whole Genome Sequencing (WGS) to simultaneously identify oral bacteria, fungi, and viruses. To correlate the HOM profile with local virus replication, the SARS-CoV-2 amount in the oral cavity was quantified by digital droplet PCR. Moreover, local inflammation and secretory immune response were also assessed, respectively by measuring the local release of pro-inflammatory cytokines (L-6, IL-17, TNFα, and GM-CSF) and the production of secretory immunoglobulins A (sIgA). The results showed the presence of oral dysbiosis in COVID-19 patients compared to matched controls, with significantly decreased alpha-diversity value and lower species richness in COVID-19 subjects. Notably, oral dysbiosis correlated with symptom severity ( |
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| Language | English |
| Publishing date | 2021-06-23 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2587354-4 |
| ISSN | 1664-302X |
| ISSN | 1664-302X |
| DOI | 10.3389/fmicb.2021.687513 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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