Article ; Online: Viral infiltration of pancreatic islets in patients with COVID-19.
2021 Volume 12, Issue 1, Page(s) 3534
Abstract: ... Here, we performed a comprehensive analysis of pancreatic autopsy tissue from COVID-19 patients using ... islet cells are permissive to infection. In eleven COVID-19 patients, we examined the expression of ACE2 ... infiltration of beta-cells in all patients. Using SARS-CoV-2 pseudoviruses, we confirmed that isolated human ...
| Abstract | Metabolic diseases are associated with an increased risk of severe COVID-19 and conversely, new-onset hyperglycemia and complications of preexisting diabetes have been observed in COVID-19 patients. Here, we performed a comprehensive analysis of pancreatic autopsy tissue from COVID-19 patients using immunofluorescence, immunohistochemistry, RNA scope and electron microscopy and detected SARS-CoV-2 viral infiltration of beta-cells in all patients. Using SARS-CoV-2 pseudoviruses, we confirmed that isolated human islet cells are permissive to infection. In eleven COVID-19 patients, we examined the expression of ACE2, TMPRSS and other receptors and factors, such as DPP4, HMBG1 and NRP1, that might facilitate virus entry. Whereas 70% of the COVID-19 patients expressed ACE2 in the vasculature, only 30% displayed ACE2-expression in beta-cells. Even in the absence of manifest new-onset diabetes, necroptotic cell death, immune cell infiltration and SARS-CoV-2 viral infection of pancreatic beta-cells may contribute to varying degrees of metabolic dysregulation in patients with COVID-19. |
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| MeSH term(s) | Adult ; Aged ; Angiotensin-Converting Enzyme 2/metabolism ; Autopsy ; COVID-19/pathology ; Diabetes Complications/pathology ; Diabetes Complications/virology ; Diabetes Mellitus/pathology ; Dipeptidyl Peptidase 4/metabolism ; Female ; HMGN Proteins/metabolism ; Humans ; Insulin-Secreting Cells/metabolism ; Insulin-Secreting Cells/virology ; Male ; Middle Aged ; Neuropilin-1/metabolism ; Organ Specificity/physiology ; Receptors, Coronavirus/metabolism ; SARS-CoV-2/isolation & purification ; Serine Endopeptidases/metabolism |
| Chemical Substances | HMGN Proteins ; NRP1 protein, human ; Receptors, Coronavirus ; Neuropilin-1 (144713-63-3) ; DPP4 protein, human (EC 3.4.14.5) ; Dipeptidyl Peptidase 4 (EC 3.4.14.5) ; ACE2 protein, human (EC 3.4.17.23) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) ; Serine Endopeptidases (EC 3.4.21.-) ; HPN protein, human (EC 3.4.21.106) |
| Language | English |
| Publishing date | 2021-06-10 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2553671-0 |
| ISSN | 2041-1723 ; 2041-1723 |
| ISSN (online) | 2041-1723 |
| ISSN | 2041-1723 |
| DOI | 10.1038/s41467-021-23886-3 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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