LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 5 of total 5

Search options

  1. Article ; Online: Engineering large viral DNA genomes using the CRISPR-Cas9 system.

    Suenaga, Tadahiro / Kohyama, Masako / Hirayasu, Kouyuki / Arase, Hisashi

    Microbiology and immunology

    2014  Volume 58, Issue 9, Page(s) 513–522

    Abstract: ... a bacterial artificial chromosome (BAC) plasmid system. However, cloning of large viral genomes into BAC plasmids is both laborious ... of the expected recombination is quite low. Alternatively, large viral genomes have been edited using ... into viral genomes. Not only gene-ablated HSV but also gene knock-in HSV were generated using this method ...

    Abstract Manipulation of viral genomes is essential for studying viral gene function and utilizing viruses for therapy. Several techniques for viral genome engineering have been developed. Homologous recombination in virus-infected cells has traditionally been used to edit viral genomes; however, the frequency of the expected recombination is quite low. Alternatively, large viral genomes have been edited using a bacterial artificial chromosome (BAC) plasmid system. However, cloning of large viral genomes into BAC plasmids is both laborious and time-consuming. In addition, because it is possible for insertion into the viral genome of drug selection markers or parts of BAC plasmids to affect viral function, artificial genes sometimes need to be removed from edited viruses. Herpes simplex virus (HSV), a common DNA virus with a genome length of 152 kbp, causes labialis, genital herpes and encephalitis. Mutant HSV is a candidate for oncotherapy, in which HSV is used to kill tumor cells. In this study, the clustered regularly interspaced short palindromic repeat-Cas9 system was used to very efficiently engineer HSV without inserting artificial genes into viral genomes. Not only gene-ablated HSV but also gene knock-in HSV were generated using this method. Furthermore, selection with phenotypes of edited genes promotes the isolation efficiencies of expectedly mutated viral clones. Because our method can be applied to other DNA viruses such as Epstein-Barr virus, cytomegaloviruses, vaccinia virus and baculovirus, our system will be useful for studying various types of viruses, including clinical isolates.
    MeSH term(s) Clustered Regularly Interspaced Short Palindromic Repeats ; DNA Viruses/genetics ; Genetic Engineering/methods ; Genome, Viral ; Humans ; Recombination, Genetic ; Simplexvirus/genetics ; Virology/methods
    Keywords covid19
    Language English
    Publishing date 2014-07-18
    Publishing country Australia
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 224792-6
    ISSN 1348-0421 ; 0385-5600
    ISSN (online) 1348-0421
    ISSN 0385-5600
    DOI 10.1111/1348-0421.12180
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.204: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: An evolved AAV variant enables efficient genetic engineering of murine T cells.

    Nyberg, William A / Ark, Jonathan / To, Angela / Clouden, Sylvanie / Reeder, Gabriella / Muldoon, Joseph J / Chung, Jing-Yi / Xie, William H / Allain, Vincent / Steinhart, Zachary / Chang, Christopher / Talbot, Alexis / Kim, Sandy / Rosales, Alan / Havlik, L Patrick / Pimentel, Harold / Asokan, Aravind / Eyquem, Justin

    Cell

    2023  Volume 186, Issue 2, Page(s) 446–460.e19

    Abstract: ... We demonstrate that Ark313 can be used for nucleofection-free DNA delivery, CRISPR-Cas9-mediated knockouts, and ... Precise targeting of large transgenes to T cells using homology-directed repair has been ... transformative for adoptive cell therapies and T cell biology. Delivery of DNA templates via ...

    Abstract Precise targeting of large transgenes to T cells using homology-directed repair has been transformative for adoptive cell therapies and T cell biology. Delivery of DNA templates via adeno-associated virus (AAV) has greatly improved knockin efficiencies, but the tropism of current AAV serotypes restricts their use to human T cells employed in immunodeficient mouse models. To enable targeted knockins in murine T cells, we evolved Ark313, a synthetic AAV that exhibits high transduction efficiency in murine T cells. We performed a genome-wide knockout screen and identified QA2 as an essential factor for Ark313 infection. We demonstrate that Ark313 can be used for nucleofection-free DNA delivery, CRISPR-Cas9-mediated knockouts, and targeted integration of large transgenes. Ark313 enables preclinical modeling of Trac-targeted CAR-T and transgenic TCR-T cells in immunocompetent models. Efficient gene targeting in murine T cells holds great potential for improved cell therapies and opens avenues in experimental T cell immunology.
    MeSH term(s) Animals ; Mice ; CRISPR-Cas Systems/genetics ; Dependovirus/genetics ; Gene Targeting ; Genetic Engineering/methods ; T-Lymphocytes
    Language English
    Publishing date 2023-01-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2022.12.022
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1167: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 58: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: Engineering T cell receptor fusion proteins using nonviral CRISPR/Cas9 genome editing for cancer immunotherapy.

    Shu, Runzhe / Hammett, Maree / Evtimov, Vera / Pupovac, Aleta / Nguyen, Nhu-Y / Islam, Rasa / Zhuang, Junli / Lee, Seyeong / Kang, Tae-Hun / Lee, Kyujun / Nisbet, Ian / Hudson, Peter / Lee, Jae Young / Boyd, Richard / Trounson, Alan

    Bioengineering & translational medicine

    2023  Volume 8, Issue 6, Page(s) e10571

    Abstract: ... of nonviral, CRISPR/Cas9 genome editing using large donor DNA delivery, knocked-in an anti-tumor ... Manufacture of chimeric antigen receptor (CAR)-T cells usually involves the use of viral delivery ... integration of the CAR into the genome, creating several disadvantages including variation in transgene ...

    Abstract Manufacture of chimeric antigen receptor (CAR)-T cells usually involves the use of viral delivery systems to achieve high transgene expression. However, it can be costly and may result in random integration of the CAR into the genome, creating several disadvantages including variation in transgene expression, functional gene silencing and potential oncogenic transformation. Here, we optimized the method of nonviral, CRISPR/Cas9 genome editing using large donor DNA delivery, knocked-in an anti-tumor single chain variable fragment (scFv) into the N-terminus of CD3ε and efficiently generated fusion protein (FP) T cells. These cells displayed FP integration within the TCR/CD3 complex, lower variability in gene expression compared to CAR-T cells and good cell expansion after transfection. CD3ε FP T cells were predominantly CD8
    Language English
    Publishing date 2023-07-10
    Publishing country United States
    Document type Journal Article
    ISSN 2380-6761
    ISSN 2380-6761
    DOI 10.1002/btm2.10571
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool.

    Gowripalan, Anjali / Smith, Stewart / Stefanovic, Tijana / Tscharke, David C

    Communications biology

    2020  Volume 3, Issue 1, Page(s) 643

    Abstract: ... for engineering genomes of large dsDNA viruses. However, for poxviruses we show that Cas9-guide RNA complexes cut ... constructs and poxvirus genomes. Instead, Cas9 cleavage leads to inhibition of poxvirus DNA ... In standard uses of CRISPR/Cas9 technology, the cutting of genomes and their efficient repair are ...

    Abstract In standard uses of CRISPR/Cas9 technology, the cutting of genomes and their efficient repair are considered to go hand-in-hand to achieve desired genetic changes. This includes the current approach for engineering genomes of large dsDNA viruses. However, for poxviruses we show that Cas9-guide RNA complexes cut viral genomes soon after their entry into cells, but repair of these breaks is inefficient. As a result, Cas9 targeting makes only modest, if any, improvements to basal rates of homologous recombination between repair constructs and poxvirus genomes. Instead, Cas9 cleavage leads to inhibition of poxvirus DNA replication thereby suppressing virus spread in culture. This unexpected outcome allows Cas9 to be used as a powerful tool for selecting conventionally generated poxvirus recombinants, which are otherwise impossible to separate from a large background of parental virus without the use of marker genes. This application of CRISPR/Cas9 greatly speeds up the generation of poxvirus-based vaccines, making this platform considerably more attractive in the context of personalised cancer vaccines and emerging disease outbreaks.
    MeSH term(s) CRISPR-Cas Systems ; Gene Expression Regulation, Viral ; Genetic Engineering ; Humans ; Vaccinia virus/genetics ; Virus Replication
    Keywords covid19
    Language English
    Publishing date 2020-11-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2399-3642
    ISSN (online) 2399-3642
    DOI 10.1038/s42003-020-01374-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: CRISPR genome engineering and viral gene delivery: a case of mutual attraction.

    Schmidt, Florian / Grimm, Dirk

    Biotechnology journal

    2015  Volume 10, Issue 2, Page(s) 258–272

    Abstract: The adaptation of the CRISPR/Cas9 DNA engineering machinery for mammalian cells has revolutionized ... Cas9 and gRNA--and hence its compatibility with virtually any available viral vector delivery system ... strategies. A large part of the attraction of CRISPR stems from the small size of its two core components ...

    Abstract The adaptation of the CRISPR/Cas9 DNA engineering machinery for mammalian cells has revolutionized our approaches to low- or high-throughput genome annotation and paved the way for conceptually novel therapeutic strategies. A large part of the attraction of CRISPR stems from the small size of its two core components--Cas9 and gRNA--and hence its compatibility with virtually any available viral vector delivery system. As a result, over the past two years, four major classes of viral vectors have already been engineered and applied as CRISPR delivery tools--retroviruses, lentiviruses, adenoviruses, and adeno-associated viruses (AAVs). The juxtaposition of these two technologies reflects a case of tremendous mutual attraction and holds unprecedented promises for biology and medicine. Here, we provide an overview of the state-of-the-art of this rapidly emerging field, from a comparative description of the principal vector designs, to a synopsis of some of the most exciting applications that were reported to date, including the use of viral CRISPR vectors for genome-wide loss-of-function screens, multiplexed gene editing or disease modeling in animals. Once specificity and safety have been improved further, viral vector-mediated in vitro/in vivo CRISPR delivery and expression promise to radically transform basic and applied biomedical research.
    MeSH term(s) Adenoviridae/genetics ; Clustered Regularly Interspaced Short Palindromic Repeats ; Dependovirus/genetics ; Gene Transfer Techniques ; Genes, Viral ; Genetic Engineering/methods ; Genetic Vectors/genetics ; Lentivirus/genetics ; Retroviridae/genetics
    Language English
    Publishing date 2015-02
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2221885-3
    ISSN 1860-7314 ; 1860-6768
    ISSN (online) 1860-7314
    ISSN 1860-6768
    DOI 10.1002/biot.201400529
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6349: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top