Article ; Online: Identification of SARS-CoV RBD-targeting monoclonal antibodies with cross-reactive or neutralizing activity against SARS-CoV-2.
2020 Volume 179, Page(s) 104820
Abstract: ... neutralizing monoclonal antibodies (nAbs) that cross-reacted with SARS-CoV-2 RBD, two of which, 18F3 and 7B11 ... to the ACE2-binding sites, explaining why 7B11 could, but 18F3 could not, block SARS-CoV or SARS-CoV-2 RBD ... whereas 7B11 recognized epitopes on SARS-CoV RBD not fully conserved in SARS-CoV-2 RBD. The 18F3-recognizing ...
| Abstract | SARS-CoV-2-caused COVID-19 cases are growing globally, calling for developing effective therapeutics to control the current pandemic. SARS-CoV-2 and SARS-CoV recognize angiotensin-converting enzyme 2 (ACE2) receptor via the receptor-binding domain (RBD). Here, we identified six SARS-CoV RBD-specific neutralizing monoclonal antibodies (nAbs) that cross-reacted with SARS-CoV-2 RBD, two of which, 18F3 and 7B11, neutralized SARS-CoV-2 infection. 18F3 recognized conserved epitopes on SARS-CoV and SARS-CoV-2 RBDs, whereas 7B11 recognized epitopes on SARS-CoV RBD not fully conserved in SARS-CoV-2 RBD. The 18F3-recognizing epitopes on RBD did not overlap with the ACE2-binding sites, whereas those recognized by 7B11 were close to the ACE2-binding sites, explaining why 7B11 could, but 18F3 could not, block SARS-CoV or SARS-CoV-2 RBD binding to ACE2 receptor. Our study provides an alternative approach to prevent SARS-CoV-2 infection using anti-SARS-CoV nAbs. |
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| MeSH term(s) | Amino Acid Sequence ; Angiotensin-Converting Enzyme 2 ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/isolation & purification ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/immunology ; Antigens, Viral/chemistry ; Antigens, Viral/genetics ; Antigens, Viral/immunology ; Betacoronavirus/genetics ; Betacoronavirus/immunology ; Binding Sites ; COVID-19 ; Coronavirus Infections/virology ; Cross Reactions ; Epitopes/immunology ; HEK293 Cells ; Humans ; Neutralization Tests ; Pandemics ; Peptidyl-Dipeptidase A/immunology ; Pneumonia, Viral/virology ; Severe acute respiratory syndrome-related coronavirus/genetics ; Severe acute respiratory syndrome-related coronavirus/immunology ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/immunology |
| Chemical Substances | Antibodies, Monoclonal ; Antibodies, Neutralizing ; Antibodies, Viral ; Antigens, Viral ; Epitopes ; Spike Glycoprotein, Coronavirus ; spike glycoprotein, SARS-CoV ; Peptidyl-Dipeptidase A (EC 3.4.15.1) ; ACE2 protein, human (EC 3.4.17.23) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-05-13 |
| Publishing country | Netherlands |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 306628-9 |
| ISSN | 1872-9096 ; 0166-3542 |
| ISSN (online) | 1872-9096 |
| ISSN | 0166-3542 |
| DOI | 10.1016/j.antiviral.2020.104820 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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