Article ; Online: Magnesium homeostasis in cardiac myocytes of Mg-deficient rats.
PloS one
2013 Volume 8, Issue 9, Page(s) e73171
Abstract: ... 2+)]i of ventricular myocytes was significantly different between Mg-deficient rats and control rats ... from the rates of change in [Mg(2+)]i during Mg loading/depletion and recovery procedures. In Mg-deficient rats ... These results suggest that [Mg(2+)]i as well as the total Mg content of cardiac myocytes, was well maintained ...
| Abstract | To study possible modulation of Mg(2+) transport in low Mg(2+) conditions, we fed either a Mg-deficient diet or a Mg-containing diet (control) to Wistar rats for 1-6 weeks. Total Mg concentrations in serum and cardiac ventricular tissues were measured by atomic absorption spectroscopy. Intracellular free Mg(2+) concentration ([Mg(2+)]i) of ventricular myocytes was measured with the fluorescent indicator furaptra. Mg(2+) transport rates, rates of Mg(2+) influx and Mg(2+) efflux, were estimated from the rates of change in [Mg(2+)]i during Mg loading/depletion and recovery procedures. In Mg-deficient rats, the serum total Mg concentration (0.29±0.026 mM) was significantly lower than in control rats (0.86±0.072 mM) after 4-6 weeks of Mg deficiency. However, neither total Mg concentration in ventricular tissues nor [Mg(2+)]i of ventricular myocytes was significantly different between Mg-deficient rats and control rats. The rates of Mg(2+) influx and efflux were not significantly different in both groups. In addition, quantitative RT-PCR revealed that Mg deficiency did not substantially change mRNA expression levels of known Mg(2+) channels/transporters (TRPM6, TRPM7, MagT1, SLC41A1 and ACDP2) in heart and kidney tissues. These results suggest that [Mg(2+)]i as well as the total Mg content of cardiac myocytes, was well maintained even under chronic hypomagnesemia without persistent modulation in function and expression of major Mg(2+) channels/transporters in the heart. |
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| MeSH term(s) | Animals ; Biological Transport ; Body Weight ; Cation Transport Proteins/genetics ; Cation Transport Proteins/metabolism ; Cell Membrane/metabolism ; Extracellular Space/metabolism ; Gene Expression ; Heart Ventricles/metabolism ; Homeostasis ; Magnesium/blood ; Magnesium/metabolism ; Magnesium Deficiency/genetics ; Magnesium Deficiency/metabolism ; Male ; Minerals/blood ; Minerals/metabolism ; Myocytes, Cardiac/metabolism ; Rats |
| Chemical Substances | Cation Transport Proteins ; Minerals ; Magnesium (I38ZP9992A) |
| Language | English |
| Publishing date | 2013-09-09 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0073171 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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