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  1. Article ; Online: Comparison of similar cells: Mesenchymal stromal cells and fibroblasts.

    Ugurlu, Burcu / Karaoz, Erdal

    Acta histochemica

    2020  Volume 122, Issue 8, Page(s) 151634

    Abstract: Almost from all organs, both mesenchymal stromal cells and fibroblasts can be isolated ... Mesenchymal stromal cells (MSCs) are the most preferred cellular therapeutic agents with the regenerative potential, and ... mesenchymal stromal cells have similar morphology, gene expression patterns, surface markers, proliferation, differentiation ...

    Abstract Almost from all organs, both mesenchymal stromal cells and fibroblasts can be isolated. Mesenchymal stromal cells (MSCs) are the most preferred cellular therapeutic agents with the regenerative potential, and fibroblasts are one of the most abundant cell types with the ability to maintain homeostasis. Because of the promising properties of MSCs, they have been well studied and their differentiation potentials, immunomodulatory potentials, gene expression profiles are identified. It has been observed that fibroblasts and mesenchymal stromal cells have similar morphology, gene expression patterns, surface markers, proliferation, differentiation, and immunomodulatory capacities. Thus, it is hard to distinguish these two cell types. Epigenetic signatures, i.e., methylation patterns of cells, are the only usable promising difference between them. Such significant similarities show that these two cells may be related to each other.
    MeSH term(s) Antigens, CD/genetics ; Antigens, CD/immunology ; Autoimmune Diseases/genetics ; Autoimmune Diseases/immunology ; Autoimmune Diseases/pathology ; Autoimmune Diseases/therapy ; Biomarkers/metabolism ; Cell Differentiation ; Cell Lineage ; Cell Proliferation ; Cell- and Tissue-Based Therapy/methods ; DNA Methylation ; Epigenesis, Genetic ; Fibroblasts/cytology ; Fibroblasts/immunology ; Fibroblasts/transplantation ; Gene Expression ; Humans ; Immunomodulation ; Mesenchymal Stem Cell Transplantation/methods ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/immunology ; Nervous System Diseases/genetics ; Nervous System Diseases/immunology ; Nervous System Diseases/pathology ; Nervous System Diseases/therapy ; Skin Diseases/genetics ; Skin Diseases/immunology ; Skin Diseases/pathology ; Skin Diseases/therapy
    Chemical Substances Antigens, CD ; Biomarkers
    Keywords covid19
    Language English
    Publishing date 2020-10-12
    Publishing country Germany
    Document type Comparative Study ; Journal Article ; Review
    ZDB-ID 77-2
    ISSN 1618-0372 ; 0065-1281
    ISSN (online) 1618-0372
    ISSN 0065-1281
    DOI 10.1016/j.acthis.2020.151634
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  2. Article ; Online: Comparison between pediatric and adult adipose mesenchymal stromal cells.

    Abbo, Olivier / Taurand, Marion / Monsarrat, Paul / Raymond, Isabelle / Arnaud, Emmanuelle / De Barros, Sandra / Auriol, Françoise / Galinier, Philippe / Casteilla, Louis / Planat-Benard, Valerie

    Cytotherapy

    2017  Volume 19, Issue 3, Page(s) 395–407

    Abstract: ... then tested in a mouse model of limb ischemia.: Results: Cells from the stromal vascular fraction (SVF) and ... of ASC from a large number of donors and showing that their in vitro and in vivo properties were similar ... were found. Cell phenotype, colony formingunit-fibroblast (CFU-F) content, immunomodulation effect and ...

    Abstract Background: Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications.
    Methods: Subcutaneous adipose tissue was collected from 27 donors (0-1 years old) and 50 donors (1-12 years old) and compared with adult ASC for in vitro characteristics. ASC were then tested in a mouse model of limb ischemia.
    Results: Cells from the stromal vascular fraction (SVF) and subsequent cultured ASC were prepared. Only a greater amount in SVF cell number and ASC proliferative rate were found. Cell phenotype, colony formingunit-fibroblast (CFU-F) content, immunomodulation effect and adipogenic, osteoblastic and angiogenic potentials were not significantly different. In vivo, pediatric ASC induced an increase in microangiographic score in a mouse model of limb ischemia, even though improvement in vascular density was not significantly correlated to limb rescue. Finally messengerRNA (mRNA) analysis using a microarray approach identified that only 305 genes were differentially expressed (217 down- and 88 up-regulated) in pediatric versus adult ASC, confirming that ASC from both age groups shared very close intrinsic properties.
    Conclusion: This is the first study reporting a comparative analysis of ASC from a large number of donors and showing that their in vitro and in vivo properties were similar and maintained during aging.
    MeSH term(s) Adult ; Age Factors ; Aging/physiology ; Animals ; Cell Differentiation/genetics ; Cells, Cultured ; Child ; Child, Preschool ; Extremities ; Female ; Humans ; Infant ; Infant, Newborn ; Ischemia/genetics ; Ischemia/pathology ; Ischemia/therapy ; Male ; Mesenchymal Stem Cell Transplantation/methods ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/metabolism ; Mice ; Mice, Nude ; Subcutaneous Fat/cytology ; Subcutaneous Fat/metabolism ; Young Adult
    Language English
    Publishing date 2017-03
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 2039821-9
    ISSN 1477-2566 ; 1465-3249
    ISSN (online) 1477-2566
    ISSN 1465-3249
    DOI 10.1016/j.jcyt.2016.11.012
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    Zs.A 5601: Show issues Location:
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  3. Article ; Online: Using human umbilical cord cells for tissue engineering: a comparison with skin cells.

    Hayward, Cindy J / Fradette, Julie / Morissette Martin, Pascal / Guignard, Rina / Germain, Lucie / Auger, François A

    Differentiation; research in biological diversity

    2014  Volume 87, Issue 3-4, Page(s) 172–181

    Abstract: ... in the context of a reconstructed skin substitute in combination with skin keratinocytes and fibroblasts ... the ability of the WJC to behave as dermal fibroblasts, producing extracellular matrix and supporting ... to skin engineered from dermal fibroblasts and keratinocytes. When cultured on dermal fibroblasts ...

    Abstract The epithelial cells and Wharton׳s jelly cells (WJC) from the human umbilical cord have yet to be extensively studied in respect to their capacity to generate tissue-engineered substitutes for clinical applications. Our reconstruction strategy, based on the self-assembly approach of tissue engineering, allows the production of various types of living human tissues such as skin and cornea from a wide range of cell types originating from post-natal tissue sources. Here we placed epithelial cells and WJC from the umbilical cord in the context of a reconstructed skin substitute in combination with skin keratinocytes and fibroblasts. We compared the ability of the epithelial cells from both sources to generate a stratified, differentiated skin-like epithelium upon exposure to air when cultured on the two stromal cell types. Conversely, the ability of the WJC to behave as dermal fibroblasts, producing extracellular matrix and supporting the formation of a differentiated epithelium for both types of epithelial cells, was also investigated. Of the four types of constructs produced, the combination of WJC and keratinocytes was the most similar to skin engineered from dermal fibroblasts and keratinocytes. When cultured on dermal fibroblasts, the cord epithelial cells were able to differentiate in vitro into a stratified multilayered epithelium expressing molecules characteristic of keratinocyte differentiation after exposure to air, and maintaining the expression of keratins K18 and K19, typical of the umbilical cord epithelium. WJC were able to support the growth and differentiation of keratinocytes, especially at the early stages of air-liquid culture. In contrast, cord epithelial cells cultured on WJC did not form a differentiated epidermis when exposed to air. These results support the premise that the tissue from which cells originate can largely affect the properties and homoeostasis of reconstructed substitutes featuring both epithelial and stromal compartments.
    MeSH term(s) Adult ; Cell Differentiation ; Cells, Cultured ; Epidermis/cytology ; Female ; Fibroblasts/cytology ; Fibroblasts/metabolism ; Humans ; Keratinocytes/cytology ; Keratinocytes/metabolism ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/metabolism ; Tissue Engineering/methods ; Umbilical Cord/cytology
    Language English
    Publishing date 2014-03
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 184540-8
    ISSN 1432-0436 ; 0301-4681
    ISSN (online) 1432-0436
    ISSN 0301-4681
    DOI 10.1016/j.diff.2014.05.001
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    Zs.A 1170: Show issues Location:
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  4. Article ; Online: Comparison between pediatric and adult adipose mesenchymal stromal cells

    Abbo, Olivier / Taurand, Marion / Monsarrat, Paul / Raymond, Isabelle / Arnaud, Emmanuelle / De Barros, Sandra / Auriol, Françoise / Galinier, Philippe / Casteilla, Louis / Planat-Benard, Valerie

    International Society for Cellular Therapy Cytotherapy. 2016,

    2016  

    Abstract: ... of limb ischemia.Cells from the stromal vascular fraction (SVF) and subsequent cultured ASC were prepared ... that their in vitro and in vivo properties were similar and maintained during aging. ... formingunit-fibroblast (CFU-F) content, immunomodulation effect and adipogenic, osteoblastic and angiogenic ...

    Abstract Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications.Subcutaneous adipose tissue was collected from 27 donors (0-1 years old) and 50 donors (1-12 years old) and compared with adult ASC for in vitro characteristics. ASC were then tested in a mouse model of limb ischemia.Cells from the stromal vascular fraction (SVF) and subsequent cultured ASC were prepared. Only a greater amount in SVF cell number and ASC proliferative rate were found. Cell phenotype, colony formingunit-fibroblast (CFU-F) content, immunomodulation effect and adipogenic, osteoblastic and angiogenic potentials were not significantly different. In vivo, pediatric ASC induced an increase in microangiographic score in a mouse model of limb ischemia, even though improvement in vascular density was not significantly correlated to limb rescue. Finally messengerRNA (mRNA) analysis using a microarray approach identified that only 305 genes were differentially expressed (217 down- and 88 up-regulated) in pediatric versus adult ASC, confirming that ASC from both age groups shared very close intrinsic properties.This is the first study reporting a comparative analysis of ASC from a large number of donors and showing that their in vitro and in vivo properties were similar and maintained during aging.
    Keywords adipose mesenchymalstromal cells ; cell therapy ; pediatric cells
    Language English
    Publishing place Elsevier Inc.
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 2039821-9
    ISSN 1477-2566 ; 1465-3249
    ISSN (online) 1477-2566
    ISSN 1465-3249
    DOI 10.1016/j.jcyt.2016.11.012
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    Zs.A 5601: Show issues Location:
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  5. Article ; Online: Comparison of Mesenchymal Stem Cell Source Differentiation Toward Human Pediatric Aortic Valve Interstitial Cells within 3D Engineered Matrices.

    Duan, Bin / Hockaday, Laura A / Das, Shoshana / Xu, Charlie / Butcher, Jonathan T

    Tissue engineering. Part C, Methods

    2015  Volume 21, Issue 8, Page(s) 795–807

    Abstract: ... expressed cell phenotype markers more similar to pHAVIC when conditioned in basic fibroblast ... expression and promoted the fibroblastic differentiation of pHAVIC, ADMSC, and BMMSC. These findings suggest ... we compared the spontaneous and induced multipotency of ADMSC and BMMSC with that of pHAVIC using different ...

    Abstract Living tissue-engineered heart valves (TEHV) would be a major benefit for children who require a replacement with the capacity for growth and biological integration. A persistent challenge for TEHV is accessible human cell source(s) that can mimic native valve cell phenotypes and matrix remodeling characteristics that are essential for long-term function. Mesenchymal stem cells derived from bone marrow (BMMSC) or adipose tissue (ADMSC) are intriguing cell sources for TEHV, but they have not been compared with pediatric human aortic valve interstitial cells (pHAVIC) in relevant 3D environments. In this study, we compared the spontaneous and induced multipotency of ADMSC and BMMSC with that of pHAVIC using different induction media within three-dimensional (3D) bioactive hybrid hydrogels with material modulus comparable to that of aortic heart valve leaflets. pHAVIC possessed some multi-lineage differentiation capacity in response to induction media, but limited to the earliest stages and much less potent than either ADMSC or BMMSC. ADMSC expressed cell phenotype markers more similar to pHAVIC when conditioned in basic fibroblast growth factor (bFGF) containing HAVIC growth medium, while BMMSC generally expressed similar extracellular matrix remodeling characteristics to pHAVIC. Finally, we covalently attached bFGF to PEG monoacrylate linkers and further covalently immobilized in the 3D hybrid hydrogels. Immobilized bFGF upregulated vimentin expression and promoted the fibroblastic differentiation of pHAVIC, ADMSC, and BMMSC. These findings suggest that stem cells retain a heightened capacity for osteogenic differentiation in 3D culture, but can be shifted toward fibroblast differentiation through matrix tethering of bFGF. Such a strategy is likely important for utilizing stem cell sources in heart valve tissue engineering applications.
    MeSH term(s) Aortic Valve/cytology ; Aortic Valve/metabolism ; Bone Marrow Cells/cytology ; Bone Marrow Cells/metabolism ; Cell Differentiation ; Cells, Cultured ; Child ; Female ; Humans ; Male ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/metabolism ; Osteogenesis
    Language English
    Publishing date 2015-08
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2420585-0
    ISSN 1937-3392 ; 1937-3384
    ISSN (online) 1937-3392
    ISSN 1937-3384
    DOI 10.1089/ten.TEC.2014.0589
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    Zs.A 5500/C: Show issues Location:
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  6. Article ; Online: Bone marrow mesenchymal stem cells stimulated by bFGF up-regulated protein expression in comparison with periodontal fibroblasts in vitro.

    Colenci, Renato / da Silva Assunção, Luciana Reichert / Mogami Bomfim, Suely Regina / de Assis Golim, Marjorie / Deffune, Elenice / Penha Oliveira, Sandra Helena

    Archives of oral biology

    2014  Volume 59, Issue 3, Page(s) 268–276

    Abstract: ... 24-h. Both collagen I and fibronectin expression were very similar between the two cells groups after ... in comparison with the expression of the same proteins in dog periodontal fibroblasts (dPLFs).: Design ... and expression of collagen type I and fibronectin of dog bone marrow mesenchymal stem cells (dBMMSCs ...

    Abstract Objective: The aim of this study was to evaluate, in vitro, the role of bFGF in the proliferation and expression of collagen type I and fibronectin of dog bone marrow mesenchymal stem cells (dBMMSCs) in comparison with the expression of the same proteins in dog periodontal fibroblasts (dPLFs).
    Design: dBMMSCs from the iliac crest were cultivated in Dulbecco's Modified Eagle's Medium (DMEM). Flow cytometry analysis (FCA) was used to characterize dBMMSC. Cells were stimulated with bFGF (1, 5 and 10 ng/mL) after 24 and 48 h. Real time RT-PCR was performed to verify collagen type I and fibronectin expressions. MTT assay was used to confirm cellular proliferation. Statistical analyses were performed (ANOVA and Kruskal-Wallis tests; p<0.05).
    Results: FCA showed 55.98% of CD34+ and 32.67% of CD90+ after bone marrow aspiration; 3.33% of CD34+ and 33.0% of CD90+ before P1. After P2, 10.54% of dBMMSCs expressed CD90, whereas after P3, this number decreased to 1.58%. dPLFs presented 4.04% of CD90+ and 1.05% of CD34+ after P3. MTT evaluation showed increase in dBMSC proliferation with 5 ng/mL bFGF-stimulus after 24-h. Both collagen I and fibronectin expression were very similar between the two cells groups after 24-h stimulation with 1 ng/mL bFGF concentration. Fibronectin and collagen I expressions were higher after 24-h stimulation with 5 ng/mL bFGF.
    Conclusion: dBMMSCs (1 ng/mL-bFGF stimulus after 24 h) are very similar to dPLFs as regards morphological and immunostaining characteristics, and collagen and/or fibronectin production. The dBMMSCs presented the highest protein expression rates with 5 ng/mL-bFGF stimulus after 24-h.
    MeSH term(s) Animals ; Bone Marrow Cells/metabolism ; Cell Proliferation/drug effects ; Collagen Type I/metabolism ; Dogs ; Fibroblast Growth Factor 2/pharmacology ; Fibroblasts/metabolism ; Fibronectins/metabolism ; Flow Cytometry ; In Vitro Techniques ; Mesenchymal Stromal Cells/metabolism ; Real-Time Polymerase Chain Reaction ; Up-Regulation
    Chemical Substances Collagen Type I ; Fibronectins ; Fibroblast Growth Factor 2 (103107-01-3)
    Language English
    Publishing date 2014-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80227-x
    ISSN 1879-1506 ; 0003-9969
    ISSN (online) 1879-1506
    ISSN 0003-9969
    DOI 10.1016/j.archoralbio.2013.11.017
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    Ul III Zs.151: Show issues Location:
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  7. Article ; Online: Niche matters: The comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulus.

    Khatun, Masuma / Sorjamaa, Anna / Kangasniemi, Marika / Sutinen, Meeri / Salo, Tuula / Liakka, Annikki / Lehenkari, Petri / Tapanainen, Juha S / Vuolteenaho, Olli / Chen, Joseph C / Lehtonen, Siri / Piltonen, Terhi T

    PloS one

    2017  Volume 12, Issue 4, Page(s) e0175986

    Abstract: ... bmMSCs to endometrial mesenchymal stem cells (eMSCs) and endometrial fibroblasts (eSFs).: Materials and ... while the differentiation process towards stromal fibroblasts seemed to result in loss of stem cell surface markers, minimal ... state.: Results: Both stem cell types, bmMSCs and eMSCs, presented with similar stem cell surface ...

    Abstract Objective: Intrinsic inflammatory characteristics play a pivotal role in stem cell recruitment and homing through migration where the subsequent change in niche has been shown to alter these characteristics. The bone marrow mesenchymal stem cells (bmMSCs) have been demonstrated to migrate to the endometrium contributing to the stem cell reservoir and regeneration of endometrial tissue. Thus, the aim of the present study was to compare the inflammation-driven migration and cytokine secretion profile of human bmMSCs to endometrial mesenchymal stem cells (eMSCs) and endometrial fibroblasts (eSFs).
    Materials and methods: The bmMSCs were isolated from bone marrow aspirates through culturing, whereas eMSCs and eSFs were FACS-isolated. All cell types were tested for their surface marker, proliferation profiles and migration properties towards serum and inflammatory attractants. The cytokine/chemokine secretion profile of 35 targets was analysed in each cell type at basal level along with lipopolysaccharide (LPS)-induced state.
    Results: Both stem cell types, bmMSCs and eMSCs, presented with similar stem cell surface marker profiles as well as possessed high proliferation and migration potential compared to eSFs. In multiplex assays, the secretion of 16 cytokine targets was detected and LPS stimulation expanded the cytokine secretion pattern by triggering the secretion of several targets. The bmMSCs exhibited higher cytokine secretion of vascular endothelial growth factor (VEGF)-A, stromal cell-derived factor-1 alpha (SDF)-1α, interleukin-1 receptor antagonist (IL-1RA), IL-6, interferon-gamma inducible protein (IP)-10, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)1α and RANTES compared to eMSCs and/or eSFs after stimulation with LPS. The basal IL-8 secretion was higher in both endometrial cell types compared to bmMSCs.
    Conclusion: Our results highlight that similar to bmMSCs, the eMSCs possess high migration activity while the differentiation process towards stromal fibroblasts seemed to result in loss of stem cell surface markers, minimal migration activity and a subtler cytokine profile likely contributing to normal endometrial function.
    MeSH term(s) Adolescent ; Adult ; Bone Marrow Cells/cytology ; Bone Marrow Cells/immunology ; CD146 Antigen/analysis ; CD146 Antigen/immunology ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Cytokines/analysis ; Cytokines/immunology ; Endometrium/cytology ; Endometrium/immunology ; Female ; Fibroblasts/cytology ; Fibroblasts/immunology ; Humans ; Inflammation/immunology ; Lipopolysaccharides/immunology ; Middle Aged ; Receptor, Platelet-Derived Growth Factor beta/analysis ; Receptor, Platelet-Derived Growth Factor beta/immunology ; Stem Cells/cytology ; Stem Cells/immunology ; Young Adult
    Chemical Substances CD146 Antigen ; Cytokines ; Lipopolysaccharides ; Receptor, Platelet-Derived Growth Factor beta (EC 2.7.10.1)
    Language English
    Publishing date 2017-04-18
    Publishing country United States
    Document type Journal Article
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0175986
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  8. Article ; Online: Characterization of menstrual stem cells: angiogenic effect, migration and hematopoietic stem cell support in comparison with bone marrow mesenchymal stem cells.

    Alcayaga-Miranda, Francisca / Cuenca, Jimena / Luz-Crawford, Patricia / Aguila-Díaz, Carolina / Fernandez, Ainoa / Figueroa, Fernando E / Khoury, Maroun

    Stem cell research & therapy

    2015  Volume 6, Page(s) 32

    Abstract: ... the characterization of MenSC populations in comparison to bone marrow derived mesenchymal stem cells (BM-MSCs ... by the secretion of vascular endothelial growth factor and basic fibroblast growth factor and also improved ... Introduction: Stem cells isolated from menstrual fluid (MenSCs) exhibit mesenchymal stem cell ...

    Abstract Introduction: Stem cells isolated from menstrual fluid (MenSCs) exhibit mesenchymal stem cell (MSCs)-like properties including multi-lineage differentiation capacity. Besides, menstrual fluid has important advantages over other sources for the isolation of MSCs, including ease of access and repeated sampling in a noninvasive manner. Such attributes allow the rapid culture of MenSCs in numbers that are sufficient for therapeutical doses, at lower cell passages.
    Methods: In this study, we advance the characterization of MenSC populations in comparison to bone marrow derived mesenchymal stem cells (BM-MSCs) with regards to proliferation, lineage differentiation, migration potential, secretion profile and angiogenic properties in vitro and in a matrigel plug assay in mice. We additionally tested their ability to support hematopoietic stem cell (HSC) expansion in vitro.
    Results: The phenotypic analysis of MenSCs revealed a profile largely similar to the BM-MSCs with the exception of a higher expression of the adhesion molecule CD49a (alpha1-integrin). Furthermore, the fibroblast colony forming units (CFU-F) from MenSCs yielded a 2 to 4 fold higher frequency of progenitors and their in vitro migration capacity was superior to BM-MSCs. In addition, MenSCs evidenced a superior paracrine response to hypoxic conditions as evidenced by the secretion of vascular endothelial growth factor and basic fibroblast growth factor and also improved angiogenic effect of conditioned media on endothelial cells. Furthermore, MenSCs were able to induce angiogenesis in a matrigel plug assay in vivo. Thus, an 8-fold increase in hemoglobin content was observed in implanted plugs containing MenSCs compared to BM-MSCs. Finally, we demonstrated, for the first time, the capacity of MenSCs to support the ex-vivo expansion of HSCs, since higher expansion rates of the CD34+CD133+ population as well as higher numbers of early progenitor (CFU-GEMM) colonies were observed in comparison to the BM source.
    Conclusions: We present evidence showing superiority of MenSCs with respect to several functional aspects, in comparison with BM-MSCs. However, the impact of such properties in their use as adult-derived stem cells for regenerative3 medicine remains to be clarified.
    MeSH term(s) Adipogenesis/physiology ; Adolescent ; Adult ; Animals ; Bone Marrow Cells/cytology ; Cell Differentiation ; Cell Lineage ; Cell Movement/physiology ; Cell Proliferation ; Cells, Cultured ; Chondrogenesis/physiology ; Endothelial Cells/cytology ; Female ; Fetal Blood/cytology ; Hematopoietic Stem Cells/cytology ; Hemoglobins/analysis ; Humans ; Leukocytes, Mononuclear/metabolism ; Menstrual Cycle/blood ; Mesenchymal Stromal Cells/cytology ; Mice ; Neovascularization, Physiologic/physiology ; Osteogenesis/physiology ; RNA/biosynthesis ; Young Adult
    Chemical Substances Hemoglobins ; RNA (63231-63-0)
    Language English
    Publishing date 2015-03-17
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2548671-8
    ISSN 1757-6512 ; 1757-6512
    ISSN (online) 1757-6512
    ISSN 1757-6512
    DOI 10.1186/s13287-015-0013-5
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  9. Article ; Online: Comparison of in vitro developmental competence of cloned caprine embryos using donor karyoplasts from adult bone marrow mesenchymal stem cells vs ear fibroblast cells.

    Kwong, P J / Nam, H Y / Wan Khadijah, W E / Kamarul, T / Abdullah, R B

    Reproduction in domestic animals = Zuchthygiene

    2014  Volume 49, Issue 2, Page(s) 249–253

    Abstract: ... derived mesenchymal stem cells (MSCs) or ear fibroblast cells (EFCs) as donor karyoplasts. Caprine MSCs ... competence, but not in their fusion and cleavage rate as compared to cloning using somatic cells such as EFCs. ... of MSCs, the cells were induced to differentiate into osteocytes, chondrocytes and adipocytes ...

    Abstract The aim of this study was to produce cloned caprine embryos using either caprine bone marrow-derived mesenchymal stem cells (MSCs) or ear fibroblast cells (EFCs) as donor karyoplasts. Caprine MSCs were isolated from male Boer goats of an average age of 1.5 years. To determine the pluripotency of MSCs, the cells were induced to differentiate into osteocytes, chondrocytes and adipocytes. Subsequently, MSCs were characterized through cell surface antigen profiles using specific markers, prior to their use as donor karyoplasts for nuclear transfer. No significant difference (p > 0.05) in fusion rates was observed between MSCs (87.7%) and EFCs (91.3%) used as donor karyoplasts. The cleavage rate of cloned embryos derived with MSCs (87.0%) was similar (p > 0.05) to those cloned using EFCs (84.4%). However, the in vitro development of MSCs-derived cloned embryos (25.3%) to the blastocyst stage was significantly higher (p < 0.05) than those derived with EFCs (20.6%). In conclusion, MSCs could be reprogrammed by caprine oocytes, and production of cloned caprine embryos with MSCs improved their in vitro developmental competence, but not in their fusion and cleavage rate as compared to cloning using somatic cells such as EFCs.
    MeSH term(s) Animals ; Bone Marrow Cells/physiology ; Cloning, Organism/methods ; Cloning, Organism/veterinary ; Ear ; Embryo Culture Techniques/methods ; Embryo Culture Techniques/veterinary ; Embryonic Development ; Female ; Fibroblasts/cytology ; Fibroblasts/physiology ; Goats/embryology ; Goats/genetics ; Male ; Mesenchymal Stromal Cells/physiology ; Nuclear Transfer Techniques/veterinary ; Oocyte Retrieval/veterinary
    Language English
    Publishing date 2014-04
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/rda.12262
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Comparison of human mesenchymal stem cells derived from dental pulp, bone marrow, adipose tissue, and umbilical cord tissue by gene expression.

    Stanko, Peter / Kaiserova, Katarina / Altanerova, Veronika / Altaner, Cestmir

    Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia

    2014  Volume 158, Issue 3, Page(s) 373–377

    Abstract: ... similar and of fibroblastoid morphology. DP-MSCs and UBC-MSCs were more proliferative than bone marrow BM ... them into two groups. While the gene expression profiles of BM-MSC, AT-MSCs and UBC-MSCs were similar, DP-MSCS ... MSCs and AT-MSCs. Protein expression of 15 genes typical for pluripotent stem cells distinguished ...

    Abstract Aims: Our aims were to characterize human mesenchymal stem cells isolated from various tissues by pluripotent stem cells gene expression profile.
    Methods: Four strains of dental pulp stem cells (DP-MSCs) were isolated from dental pulp tissue fragments adhered to plastic tissue culture dishes. Mesenchymal stem cells derived from umbilical cord tissue (UBC-MSCs) were isolated with the same technique. Bone marrow derived mesenchymal stem cells (BM-MSCs) were isolated from nucleated cells of bone marrow obtained by density gradient centrifugation. Human mesenchymal stem cells from adipose tissue (AT-MSCs) were isolated by collagenase digestion. All kinds of MSCs used in this study were cultivated in low glucose DMEM containing 5% or human platelet extract. All stem cell manipulation was performed in GMP conditions. Expression of 15 pluripotent stem cells genes on the level of proteins was measured by Proteome Profiler Human Pluripotent Stem Cell Array. Induction of MSCs to in vitro differentiation to adipocytes, osteoblasts, chondroblasts was achieved by cultivation of cells in appropriate differentiation medium.
    Results: All MSCs tested were phenotypically similar and of fibroblastoid morphology. DP-MSCs and UBC-MSCs were more proliferative than bone marrow BM-MSCs and AT-MSCs. Protein expression of 15 genes typical for pluripotent stem cells distinguished them into two groups. While the gene expression profiles of BM-MSC, AT-MSCs and UBC-MSCs were similar, DP-MSCS differed in relative gene expression on the level of their products in several genes.
    Conclusions: Dental pulp mesenchymal stem cells cultivated in vitro under the same conditions as MSCs from bone marrow, adipose tissue and umbilical cord tissue can be distinguished by pluripotent stem cell gene expression profile.
    MeSH term(s) Adipose Tissue/cytology ; Bone Marrow Cells/cytology ; Cell Culture Techniques ; Cell Differentiation/physiology ; Cell Proliferation ; Cells, Cultured ; Dental Pulp/cytology ; Gene Expression ; Humans ; In Vitro Techniques ; Mesenchymal Stromal Cells/cytology ; Phenotype ; Umbilical Cord/cytology
    Language English
    Publishing date 2014-09
    Publishing country Czech Republic
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 17196-7
    ISSN 1804-7521 ; 1213-8118 ; 0231-5599 ; 0862-481X
    ISSN (online) 1804-7521
    ISSN 1213-8118 ; 0231-5599 ; 0862-481X
    DOI 10.5507/bp.2013.078
    Database MEDical Literature Analysis and Retrieval System OnLINE

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