Article ; Online: IMMUNO-COV v2.0: Development and Validation of a High-Throughput Clinical Assay for Measuring SARS-CoV-2-Neutralizing Antibody Titers.
mSphere
2021 Volume 6, Issue 3, Page(s) e0017021
Abstract: ... validated high-throughput assays for measuring titers of SARS-CoV-2-neutralizing antibodies are not ... generally available. Here, we describe the development and validation of IMMUNO-COV v2.0, a scalable ... titers, calculated using a standard curve consisting of stepped concentrations of SARS-CoV-2 spike ...
| Abstract | Neutralizing antibodies are key determinants of protection from future infection, yet well-validated high-throughput assays for measuring titers of SARS-CoV-2-neutralizing antibodies are not generally available. Here, we describe the development and validation of IMMUNO-COV v2.0, a scalable surrogate virus assay, which titrates antibodies that block infection of Vero-ACE2 cells by a luciferase-encoding vesicular stomatitis virus displaying SARS-CoV-2 spike glycoproteins (VSV-SARS2-Fluc). Antibody titers, calculated using a standard curve consisting of stepped concentrations of SARS-CoV-2 spike monoclonal antibody, correlated closely ( |
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| MeSH term(s) | Animals ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; Chlorocebus aethiops ; High-Throughput Screening Assays/methods ; Humans ; Limit of Detection ; Neutralization Tests/methods ; Severity of Illness Index ; Vero Cells |
| Chemical Substances | Antibodies, Neutralizing ; Antibodies, Viral |
| Language | English |
| Publishing date | 2021-06-02 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Validation Study |
| ISSN | 2379-5042 |
| ISSN (online) | 2379-5042 |
| DOI | 10.1128/mSphere.00170-21 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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