Article: Pulmonary surfactant protein A binds to Cryptococcus neoformans without promoting phagocytosis.
European journal of clinical investigation
1999 Volume 29, Issue 1, Page(s) 83–92
Abstract: ... viruses. The ability of SP-A to facilitate the binding and uptake of acapsular Cryptococcus neoformans ... that SP-A binds in a concentration-dependent manner to both encapsulated and acapsular C. neoformans ... Background: Evidence is accumulating that the alveolar collecting surfactant protein A (SP ...
| Abstract | Background: Evidence is accumulating that the alveolar collecting surfactant protein A (SP-A) plays an important role in the first line of defence against infiltrating pathogenic micro-organisms and viruses. The ability of SP-A to facilitate the binding and uptake of acapsular Cryptococcus neoformans by monocyte-derived macrophages, human alveolar macrophages, monocytes and polymorphonuclear leucocytes was investigated. Materials and methods: Binding, competition and phagocytosis experiments were performed using a flow cytometry technique. Results: SP-A bound to both the acapsular and the encapsulated form of C. neoformans in a concentration-dependent manner. SP-A showed a threefold better binding to the acapsular yeast: this binding was partly calcium dependent and could be inhibited by mannose (ID50 = 3 mmol L-1) and glucose (ID50 = 2.1 mmol L-1) but not by galactose (ID50 = 391 mmol L-1). SP-A did not function as an opsonin in phagocytosis of acapsular C. neoformans for any of the phagocytes studied. Conclusion: Our results indicate that SP-A binds in a concentration-dependent manner to both encapsulated and acapsular C. neoformans. Despite SP-A binding to the acapsular C. neoformans, phagocytosis by various phagocytes was not enhanced. |
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| MeSH term(s) | Animals ; Aspergillus fumigatus ; Binding Sites ; Calcium/pharmacology ; Carbohydrate Metabolism ; Cryptococcus neoformans/metabolism ; Galactose/pharmacology ; Glucose/pharmacology ; Glycoproteins/metabolism ; Humans ; Influenza A virus ; Mannose/pharmacology ; Opsonin Proteins ; Phagocytes ; Phagocytosis ; Protein Binding/drug effects ; Proteolipids/metabolism ; Pulmonary Surfactant-Associated Protein A ; Pulmonary Surfactant-Associated Protein D ; Pulmonary Surfactant-Associated Proteins ; Pulmonary Surfactants/metabolism ; Rats | |||||
| Chemical Substances | Glycoproteins ; Opsonin Proteins ; Proteolipids ; Pulmonary Surfactant-Associated Protein A ; Pulmonary Surfactant-Associated Protein D ; Pulmonary Surfactant-Associated Proteins ; Pulmonary Surfactants ; Glucose (IY9XDZ35W2) ; Mannose (PHA4727WTP) ; Calcium (SY7Q814VUP) ; Galactose (X2RN3Q8DNE) | |||||
| Language | English | |||||
| Publishing date | 1999-01 | |||||
| Publishing country | England | |||||
| Document type | Comparative Study ; Journal Article | |||||
| ZDB-ID | 186196-7 | |||||
| ISSN | 1365-2362 ; 0014-2972 ; 0960-135X | |||||
| ISSN (online) | 1365-2362 | |||||
| ISSN | 0014-2972 ; 0960-135X | |||||
| DOI | 10.1046/j.1365-2362.1999.00429.x | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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