Article ; Online: Migration of myeloid cells during inflammation is differentially regulated by the cell surface receptors Slamf1 and Slamf8.
PloS one
2015 Volume 10, Issue 3, Page(s) e0121968
Abstract: ... migration. We conclude that Slamf1 and Slamf8 govern ROS-dependent innate immune responses of myeloid cells ... macrophages and neutrophils is accelerated. These opposing effects of Slamf1 and Slamf8 are cell-intrinsic ... Previous studies have demonstrated that the cell surface receptor Slamf1 (CD150) is requisite ...
| Abstract | Previous studies have demonstrated that the cell surface receptor Slamf1 (CD150) is requisite for optimal NADPH-oxidase (Nox2) dependent reactive oxygen species (ROS) production by phagocytes in response to Gram- bacteria. By contrast, Slamf8 (CD353) is a negative regulator of ROS in response to Gram+ and Gram- bacteria. Employing in vivo migration after skin sensitization, induction of peritonitis, and repopulation of the small intestine demonstrates that in vivo migration of Slamf1-/- dendritic cells and macrophages is reduced, as compared to wt mice. By contrast, in vivo migration of Slamf8-/- dendritic cells, macrophages and neutrophils is accelerated. These opposing effects of Slamf1 and Slamf8 are cell-intrinsic as judged by in vitro migration in transwell chambers in response to CCL19, CCL21 or CSF-1. Importantly, inhibiting ROS production of Slamf8-/- macrophages by diphenyleneiodonium chloride blocks this in vitro migration. We conclude that Slamf1 and Slamf8 govern ROS-dependent innate immune responses of myeloid cells, thus modulating migration of these cells during inflammation in an opposing manner. |
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| MeSH term(s) | Animals ; Antigens, CD/genetics ; Antigens, CD/metabolism ; Antigens, CD/physiology ; Cell Movement/genetics ; Cell Movement/physiology ; Chemotaxis/drug effects ; Dendritic Cells/cytology ; Dendritic Cells/metabolism ; Macrophages/cytology ; Macrophages/metabolism ; Membrane Proteins ; Mice ; Mice, Inbred BALB C ; Myeloid Cells/cytology ; Myeloid Cells/metabolism ; Neutrophils/cytology ; Neutrophils/metabolism ; Peritonitis/metabolism ; Peritonitis/pathology ; Reactive Oxygen Species/metabolism ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/metabolism ; Receptors, Cell Surface/physiology ; Signaling Lymphocytic Activation Molecule Family Member 1 |
| Chemical Substances | Antigens, CD ; Membrane Proteins ; Reactive Oxygen Species ; Receptors, Cell Surface ; SLAM family member 8 protein, mouse ; Slamf1 protein, mouse ; Signaling Lymphocytic Activation Molecule Family Member 1 (169535-43-7) |
| Language | English |
| Publishing date | 2015-03-23 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0121968 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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