Article ; Online: Development of a SARS-CoV-2 rapid antibody detection kit and study on dynamic changes in antibodies in infected patients.
The clinical respiratory journal
2021 Volume 15, Issue 5, Page(s) 499–505
Abstract: ... To develop a rapid detection kit for new coronavirus antibodies and use it to study the dynamic changes ... in antibodies in clinically confirmed SARS-CoV-2-infected patients.: Methods: The SARS-CoV-2 IgM/IgG antibody ... of the IgM, IgG and IgM + IgG antibodies were all 100%. Using the SARS-CoV-2 IgM/IgG antibody test kit ...
| Abstract | Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly to 185 regions and countries around the world with more than 2.8 million confirmed infections and 203,044 deaths. Respiratory diseases caused by SARS-CoV-2 are serious threats to human health. Objectives: To develop a rapid detection kit for new coronavirus antibodies and use it to study the dynamic changes in antibodies in clinically confirmed SARS-CoV-2-infected patients. Methods: The SARS-CoV-2 IgM/IgG antibody test kit (colloidal gold method) was developed. Serum SARS-CoV-2 IgM and IgG antibodies were tested in SARS-CoV-2- and non-SARS-CoV-2-infected persons, respectively. Results and conclusion: The sensitivities of the SARS-CoV-2 IgM/IgG antibody test kit (colloidal gold method) were 50%, 70%, 92.5% and 97.5% after 1-3 days, 4-6 days, 7-9 days and >9 days of admission, respectively, and the specificities of the IgM, IgG and IgM + IgG antibodies were all 100%. Using the SARS-CoV-2 IgM/IgG antibody test kit (colloidal gold method), the positive rates of SARS-CoV-2 IgM and IgG antibodies increased from 50% to 92.5% after 1-3 days, 4-6 days and 7-9 days of admission, which showed an increasing trend. The titers of the SARS-CoV-2 IgM and IgG antibodies in the positive specimens increased with the length of admission. |
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| MeSH term(s) | Adult ; Antibodies, Viral/blood ; COVID-19/diagnosis ; COVID-19/epidemiology ; Female ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Immunoglobulin M/immunology ; Male ; Pandemics ; SARS-CoV-2/immunology |
| Chemical Substances | Antibodies, Viral ; Immunoglobulin G ; Immunoglobulin M |
| Language | English |
| Publishing date | 2021-02-04 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2442214-9 |
| ISSN | 1752-699X ; 1752-6981 |
| ISSN (online) | 1752-699X |
| ISSN | 1752-6981 |
| DOI | 10.1111/crj.13331 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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