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  1. Article ; Online: Caveolin 1 Promotes Renal Water and Salt Reabsorption.

    Willière, Yan / Borschewski, Aljona / Patzak, Andreas / Nikitina, Tatiana / Dittmayer, Carsten / Daigeler, Anna L / Schuelke, Markus / Bachmann, Sebastian / Mutig, Kerim

    Scientific reports

    2018  Volume 8, Issue 1, Page(s) 545

    Abstract: ... about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water ... that renal caveolae help to conserve water and electrolytes via modulation of NCC function and regulation ... reabsorption. Wild-type (WT) and Cav1-deficient (Cav1-/-) mice were studied. Cav1 expression and caveolae ...

    Abstract Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1-/-) mice were studied. Cav1 expression and caveolae formation were present in vascular cells, late distal convoluted tubule and principal connecting tubule and collecting duct cells of WT but not Cav1-/- kidneys. Urinary sodium excretion was increased by 94% and urine flow by 126% in Cav1-/- mice (p < 0.05). A decrease in activating phosphorylation of the Na-Cl cotransporter (NCC) of the distal convoluted tubule was recorded in Cav1-/- compared to WT kidneys (-40%; p < 0.05). Isolated intrarenal arteries from Cav1-/- mice revealed a fourfold reduction in sensitivity to phenylephrine (p < 0.05). A significantly diminished maximal contractile response (-13%; p < 0.05) was suggestive of enhanced nitric oxide (NO) availability. In line with this, the abundance of endothelial NO synthase (eNOS) was increased in Cav1-/- kidneys +213%; p < 0.05) and cultured caveolae-deprived cells showed intracellular accumulation of eNOS, compared to caveolae-intact controls. Our results suggest that renal caveolae help to conserve water and electrolytes via modulation of NCC function and regulation of vascular eNOS.
    MeSH term(s) Animals ; Caveolin 1/genetics ; Caveolin 1/metabolism ; Cells, Cultured ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/physiology ; Humans ; Kidney/blood supply ; Kidney/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type III/metabolism ; Renal Artery/metabolism ; Renal Artery/physiology ; Renal Reabsorption ; Sodium/metabolism ; Sodium-Calcium Exchanger/metabolism
    Chemical Substances Cav1 protein, mouse ; Caveolin 1 ; Sodium-Calcium Exchanger ; Nitric Oxide (31C4KY9ESH) ; Sodium (9NEZ333N27) ; Nitric Oxide Synthase Type III (EC 1.14.13.39)
    Language English
    Publishing date 2018-01-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-19071-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Caveolin 1 Promotes Renal Water and Salt Reabsorption

    Yan Willière / Aljona Borschewski / Andreas Patzak / Tatiana Nikitina / Carsten Dittmayer / Anna L. Daigeler / Markus Schuelke / Sebastian Bachmann / Kerim Mutig

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Volume 14

    Abstract: ... about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water ... that renal caveolae help to conserve water and electrolytes via modulation of NCC function and regulation ... reabsorption. Wild-type (WT) and Cav1-deficient (Cav1−/−) mice were studied. Cav1 expression and caveolae ...

    Abstract Abstract Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1−/−) mice were studied. Cav1 expression and caveolae formation were present in vascular cells, late distal convoluted tubule and principal connecting tubule and collecting duct cells of WT but not Cav1−/− kidneys. Urinary sodium excretion was increased by 94% and urine flow by 126% in Cav1−/− mice (p < 0.05). A decrease in activating phosphorylation of the Na-Cl cotransporter (NCC) of the distal convoluted tubule was recorded in Cav1−/− compared to WT kidneys (−40%; p < 0.05). Isolated intrarenal arteries from Cav1−/− mice revealed a fourfold reduction in sensitivity to phenylephrine (p < 0.05). A significantly diminished maximal contractile response (−13%; p < 0.05) was suggestive of enhanced nitric oxide (NO) availability. In line with this, the abundance of endothelial NO synthase (eNOS) was increased in Cav1−/− kidneys +213%; p < 0.05) and cultured caveolae-deprived cells showed intracellular accumulation of eNOS, compared to caveolae-intact controls. Our results suggest that renal caveolae help to conserve water and electrolytes via modulation of NCC function and regulation of vascular eNOS.
    Keywords Medicine ; R ; Science ; Q
    Subject code 616
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor.

    Inthachart, Kevalin / Manotham, Krissanapong / Eiam-Ong, Somchai / Eiam-Ong, Somchit

    Endocrinology and metabolism (Seoul, Korea)

    2019  Volume 34, Issue 3, Page(s) 291–301

    Abstract: ... associated with salt-sensitive high blood pressure and promote renal sodium and water reabsorption ... Background: Striatin and caveolin-1 (cav-1) are scaffolding/regulating proteins that are ... respectively. The mineralocorticoid receptor (MR) interacts with striatin and cav-1, while aldosterone ...

    Abstract Background: Striatin and caveolin-1 (cav-1) are scaffolding/regulating proteins that are associated with salt-sensitive high blood pressure and promote renal sodium and water reabsorption, respectively. The mineralocorticoid receptor (MR) interacts with striatin and cav-1, while aldosterone increases striatin and cav-1 levels. However, no
    Methods: Male Wistar rats were intraperitoneally injected with normal saline solution, aldosterone alone (Aldo: 150 μg/kg body weight), or aldosterone after pretreatment with eplerenone, an MR blocker, 30 minutes before the aldosterone injection (eplerenone [Ep.]+Aldo). Thirty minutes after the aldosterone injection, the amount and localization of striatin and cav-1 were determined by Western blot analysis and immunohistochemistry, respectively.
    Results: Aldosterone increased striatin levels by 150% (
    Conclusion: This is the first
    MeSH term(s) Aldosterone/administration & dosage ; Aldosterone/metabolism ; Animals ; Calmodulin-Binding Proteins/metabolism ; Caveolin 1/metabolism ; Kidney/drug effects ; Kidney/metabolism ; Male ; Membrane Proteins/metabolism ; Nerve Tissue Proteins/metabolism ; Rats, Wistar ; Receptors, Mineralocorticoid/metabolism
    Chemical Substances Calmodulin-Binding Proteins ; Cav1 protein, rat ; Caveolin 1 ; Membrane Proteins ; Nerve Tissue Proteins ; Receptors, Mineralocorticoid ; Strn protein, rat ; Aldosterone (4964P6T9RB)
    Language English
    Publishing date 2019-09-29
    Publishing country Korea (South)
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2802452-7
    ISSN 2093-5978 ; 2093-596X
    ISSN (online) 2093-5978
    ISSN 2093-596X
    DOI 10.3803/EnM.2019.34.3.291
    Database MEDical Literature Analysis and Retrieval System OnLINE

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