Article ; Online: Brief reports: TRPM7 Senses mechanical stimulation inducing osteogenesis in human bone marrow mesenchymal stem cells.
2015 Volume 33, Issue 2, Page(s) 615–621
Abstract: Mesenchymal stem cells (MSCs) are multipotential stem cells residing in the bone marrow ... localization and osteogenesis. These results identified a central role of TRPM7 in MSC mechanical stimulation ... in mechanotransduction in bone marrow MSCs. TRPM7 knockdown completely abolished the pressure-induced cytosolic Ca(2+ ...
| Abstract | Mesenchymal stem cells (MSCs) are multipotential stem cells residing in the bone marrow. Several studies have shown that mechanical stimulation modulates MSC differentiation through mobilization of second messengers, but the mechanism of mechanotransduction remains poorly understood. In this study, using fluorescence and laser confocal microcopy as well as patch-clamp techniques, we identified the transient receptor potential melastatin type 7 (TRPM7) channel as the key channel involved in mechanotransduction in bone marrow MSCs. TRPM7 knockdown completely abolished the pressure-induced cytosolic Ca(2+) increase and pressure-induced osteogenesis. TRPM7 directly sensed membrane tension, independent of the cytoplasm and the integrity of cytoskeleton. Ca(2+) influx through TRPM7 further triggered Ca(2+) release from the inositol trisphosphate receptor type 2 on the endoplasmic reticulum and promoted NFATc1 nuclear localization and osteogenesis. These results identified a central role of TRPM7 in MSC mechanical stimulation-induced osteogenesis. |
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| MeSH term(s) | Bone Marrow Cells/cytology ; Bone Marrow Cells/metabolism ; Cells, Cultured ; Humans ; Mechanotransduction, Cellular/physiology ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/metabolism ; Osteogenesis ; Pressure ; Protein-Serine-Threonine Kinases/metabolism ; TRPM Cation Channels/metabolism | |||||
| Chemical Substances | TRPM Cation Channels ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; TRPM7 protein, human (EC 2.7.11.1) | |||||
| Language | English | |||||
| Publishing date | 2015-02 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 1143556-2 | |||||
| ISSN | 1549-4918 ; 1066-5099 | |||||
| ISSN (online) | 1549-4918 | |||||
| ISSN | 1066-5099 | |||||
| DOI | 10.1002/stem.1858 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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