Article ; Online: Expression of endoplasmic reticulum stress-related factors in the retinas of diabetic rats.
Experimental diabetes research
2011 Volume 2012, Page(s) 743780
Abstract: ... ER stress-related factors was found in the retinas of diabetic rats, at both 1- and 3-months after ... changes in expression levels of 13 and 12 ER stress-related factors in the diabetic rat retinas ... Western blot analyses showed that the expression of HERP was reduced in the retinas of diabetic rats in first and third ...
| Abstract | Recent reports show that ER stress plays an important role in diabetic retinopathy (DR), but ER stress is a complicated process involving a network of signaling pathways and hundreds of factors, What factors involved in DR are not yet understood. We selected 89 ER stress factors from more than 200, A rat diabetes model was established by intraperitoneal injection of streptozotocin (STZ). The expression of 89 ER stress-related factors was found in the retinas of diabetic rats, at both 1- and 3-months after development of diabetes, by quantitative real-time polymerase chain reaction arrays. There were significant changes in expression levels of 13 and 12 ER stress-related factors in the diabetic rat retinas in the first and third month after the development of diabetes, Based on the array results, homocysteine- inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1(HERP), and synoviolin(HRD1) were studied further by immunofluorescence and Western blot. Immunofluorescence and Western blot analyses showed that the expression of HERP was reduced in the retinas of diabetic rats in first and third month. The expression of Hrd1 did not change significantly in the retinas of diabetic rats in the first month but was reduced in the third month. |
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| MeSH term(s) | Animals ; Cells, Cultured ; Diabetes Mellitus, Experimental/genetics ; Diabetes Mellitus, Experimental/metabolism ; Diabetic Retinopathy/genetics ; Diabetic Retinopathy/metabolism ; Endoplasmic Reticulum Stress/genetics ; Endoplasmic Reticulum Stress/physiology ; Male ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction ; Retina/metabolism ; Retinal Vessels/metabolism ; Signal Transduction ; Time Factors ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism |
| Chemical Substances | RNA, Messenger ; Syvn1 protein, rat (EC 2.3.2.27) ; Ubiquitin-Protein Ligases (EC 2.3.2.27) |
| Language | English |
| Publishing date | 2011-08-28 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2465179-5 |
| ISSN | 1687-5303 ; 1687-5214 |
| ISSN (online) | 1687-5303 |
| ISSN | 1687-5214 |
| DOI | 10.1155/2012/743780 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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