Article ; Online: Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV.
Journal of clinical laboratory analysis
2017 Volume 32, Issue 2
Abstract: ... RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ ... of three multiplex RT-PCR assays for the detection of respiratory viruses.: Methods: A total of 201 ... between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison ...
| Abstract | Background: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. Methods: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. Results: Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. Conclusions: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting. |
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| MeSH term(s) | Adolescent ; Adult ; Humans ; Male ; Molecular Diagnostic Techniques/methods ; Multiplex Polymerase Chain Reaction/methods ; Reproducibility of Results ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/virology ; Sensitivity and Specificity ; Virus Diseases/diagnosis ; Virus Diseases/virology ; Viruses/genetics ; Young Adult | |||||
| Keywords | covid19 | |||||
| Language | English | |||||
| Publishing date | 2017-04-11 | |||||
| Publishing country | United States | |||||
| Document type | Comparative Study ; Journal Article | |||||
| ZDB-ID | 645095-7 | |||||
| ISSN | 1098-2825 ; 0887-8013 | |||||
| ISSN (online) | 1098-2825 | |||||
| ISSN | 0887-8013 | |||||
| DOI | 10.1002/jcla.22230 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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