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  1. Article ; Online: Role of the lncRNA-p53 regulatory network in cancer.

    Zhang, Ali / Xu, Min / Mo, Yin-Yuan

    Journal of molecular cell biology

    2014  Volume 6, Issue 3, Page(s) 181–191

    Abstract: ... in the p53 tumor suppressor regulatory network. In this review, we discuss how lncRNAs serve as p53 ... a better understanding of lncRNA-mediated gene regulation in the p53 pathway. As a result, lncRNAs ... regulators or p53 effectors. Further characterization of these p53-associated lncRNAs in cancer will provide ...

    Abstract Advances in functional genomics have led to discovery of a large group of previous uncharacterized long non-coding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs may serve as master gene regulators through various mechanisms. Dysregulation of lncRNAs is often associated with a variety of human diseases including cancer. Of significant interest, recent studies suggest that lncRNAs participate in the p53 tumor suppressor regulatory network. In this review, we discuss how lncRNAs serve as p53 regulators or p53 effectors. Further characterization of these p53-associated lncRNAs in cancer will provide a better understanding of lncRNA-mediated gene regulation in the p53 pathway. As a result, lncRNAs may prove to be valuable biomarkers for cancer diagnosis or potential targets for cancer therapy.
    MeSH term(s) Gene Expression Regulation, Neoplastic ; Gene Regulatory Networks ; Heterogeneous-Nuclear Ribonucleoproteins/metabolism ; Humans ; Neoplasms/genetics ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; Tumor Suppressor Protein p53/genetics
    Chemical Substances Heterogeneous-Nuclear Ribonucleoproteins ; RNA, Long Noncoding ; Tumor Suppressor Protein p53
    Language English
    Publishing date 2014-04-09
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2500949-7
    ISSN 1759-4685 ; 1674-2788
    ISSN (online) 1759-4685
    ISSN 1674-2788
    DOI 10.1093/jmcb/mju013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Bioinformatics analysis proposes a possible role for long noncoding RNA MIR17HG in retinoblastoma.

    Wang, Zijin / Liang, Xiaotian / Yi, Guoguo / Wu, Tong / Sun, Yuxin / Zhang, Ziran / Fu, Min

    Cancer reports (Hoboken, N.J.)

    2024  Volume 7, Issue 2, Page(s) e1933

    Abstract: ... analyse the function of mRNAs and lncRNAs, and finds the relevant regulatory network.: Methods and ... functions of lncRNA and mRNA networks. Weighted gene co-expression network analysis (WGCNA) is employed ... a significant correlation between the lncRNA MIR17HG (R = .73, p = .02) and the RB phenotype. ceRNA networks ...

    Abstract Background: Retinoblastoma (RB) is the most common prevalent intraocular malignancy among infants and children, particularly in underdeveloped countries. With advancements in genomics and transcriptomics, noncoding RNAs have been increasingly utilized to investigate the molecular pathology of diverse diseases.
    Aims: This study aims to establish the competing endogenous RNAs network associated with RB, analyse the function of mRNAs and lncRNAs, and finds the relevant regulatory network.
    Methods and results: This study establishes a network of competing endogenous RNAs by Spearman correlation analysis and prediction based on RB patients and healthy children. Enrichment analyzes based on Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes are conducted to analyze the potential biological functions of lncRNA and mRNA networks. Weighted gene co-expression network analysis (WGCNA) is employed to identify gene cluster modules exhibiting the strongest correlation with RB. The results indicate a significant correlation between the lncRNA MIR17HG (R = .73, p = .02) and the RB phenotype. ceRNA networks reveal downstream miRNAs (hsa-mir-425-5p and hsa-mir455-5p) and mRNAs (MDM2, IPO11, and ITGA1) associated with MIR17Hg. As an inhibitor of the p53 signaling pathway, MDM2 can suppress the development of RB.
    Conclusion: In conclusion, lncRNAs play a role in RB, and the MIR17HG/hsa-mir-425-5p/MDM2 pathway may contribute to RB development by inhibiting the p53 signaling pathway.
    MeSH term(s) Child ; Humans ; Infant ; beta Karyopherins ; Computational Biology/methods ; MicroRNAs/genetics ; Retinal Neoplasms/genetics ; Retinoblastoma/genetics ; RNA, Long Noncoding/genetics ; RNA, Messenger/genetics ; Tumor Suppressor Protein p53
    Chemical Substances beta Karyopherins ; IPO11 protein, human ; MicroRNAs ; MIRN455 microRNA, human ; RNA, Long Noncoding ; RNA, Messenger ; Tumor Suppressor Protein p53 ; MIR17HG, human
    Language English
    Publishing date 2024-02-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2573-8348
    ISSN (online) 2573-8348
    DOI 10.1002/cnr2.1933
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Roles of H19/miR-29a-3p/COL1A1 axis in COE-induced lung cancer.

    Zhang, Heng / Li, Xinmei / Jia, Mengmeng / Ji, Jing / Wu, Zhaoxu / Chen, Xian / Yu, Dianke / Zheng, Yuxin / Zhao, Yanjie

    Environmental pollution (Barking, Essex : 1987)

    2022  Volume 313, Page(s) 120194

    Abstract: ... regulatory roles in cancers. In this study, we aimed to construct and verify the ceRNA regulatory network ... miRNAs and 146 mRNAs were identified. Among these, we constructed a ceRNA network including 1 lncRNA, 2 ... western-blot were used to verify the regulatory axis. CCK8 assay, phalloidin staining, p53 detection were used ...

    Abstract Occupational lung cancer caused by coke oven emissions (COE) has attracted increasing attention, but the mechanism is not clear. Many evidences show ceRNA (competing endogenous RNA) networks play important regulatory roles in cancers. In this study, we aimed to construct and verify the ceRNA regulatory network in the occurrence of COE-induced lung squamous cell carcinoma (LUSC). We performed RNA sequencing with lung bronchial epithelial cell (16HBE) and COE induced malignant transformed cell (Rf). Furthermore, we analyzed RNA sequencing data of LUSC and adjacent tissues in the cancer genome atlas (TCGA) database. Combined our data and TCGA data to determine the differentially expressed lncRNAs, miRNAs, mRNAs. lncBASE, miRDB and miRTarBase were used to predict the binding relationship between lncRNA and miRNA, miRNA and mRNA. Based on these, we construct the ceRNA network. FREMSA, dual-luciferase reporter assay, quantitative real-time PCR (qRT-PCR), western-blot were used to verify the regulatory axis. CCK8 assay, phalloidin staining, p53 detection were used to explore the roles of this axis in the COE induced malignant transformation. Results showed 7 lncRNAs, 7 miRNAs and 146 mRNAs were identified. Among these, we constructed a ceRNA network including 1 lncRNA, 2 miRNAs and 9 mRNAs. Further verification confirmed the trend of lncRNA H19, miR-29a-3p and COL1A1 were consistent with sequencing results. H19 and COL1A1 were significantly higher in Rf than in 16HBE and miR-29a-3p was reverse. Regulatory investigation revealed H19 increased COL1A1 expression by sponging miR-29a-3p. Knockdown of H19, COL1A1 or overexpression of miR-29a-3p in Rf cells could inhibit cell proliferation, increased cell adhesion and p53 level. However, knockdown of H19 while suppressing the miR-29a-3p partially rescue the malignant phenotype of Rf caused by H19. In conclusion, all these indicated H19 functioned as a ceRNA to increase COL1A1 by sponging miR-29a-3p and promoted COE-induced cell malignant transformation.
    MeSH term(s) Carcinoma, Squamous Cell ; Coke ; Collagen Type I, alpha 1 Chain/metabolism ; Humans ; Lung Neoplasms/genetics ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Phalloidine/metabolism ; RNA, Long Noncoding/genetics ; RNA, Messenger/genetics ; Tumor Suppressor Protein p53
    Chemical Substances Coke ; Collagen Type I, alpha 1 Chain ; H19 long non-coding RNA ; MIRN29a microRNA, human ; MicroRNAs ; RNA, Long Noncoding ; RNA, Messenger ; Tumor Suppressor Protein p53 ; Phalloidine (17466-45-4)
    Language English
    Publishing date 2022-09-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 280652-6
    ISSN 1873-6424 ; 0013-9327 ; 0269-7491
    ISSN (online) 1873-6424
    ISSN 0013-9327 ; 0269-7491
    DOI 10.1016/j.envpol.2022.120194
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2599: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Roles of H19/miR-29a-3p/COL1A1 axis in COE-induced lung cancer

    Zhang, Heng / Li, Xinmei / Jia, Mengmeng / Ji, Jing / Wu, Zhaoxu / Chen, Xian / Yu, Dianke / Zheng, Yuxin / Zhao, Yanjie

    Environmental pollution. 2022 Nov. 15, v. 313

    2022  

    Abstract: ... regulatory roles in cancers. In this study, we aimed to construct and verify the ceRNA regulatory network ... miRNAs and 146 mRNAs were identified. Among these, we constructed a ceRNA network including 1 lncRNA, 2 ... western-blot were used to verify the regulatory axis. CCK8 assay, phalloidin staining, p53 detection were used ...

    Abstract Occupational lung cancer caused by coke oven emissions (COE) has attracted increasing attention, but the mechanism is not clear. Many evidences show ceRNA (competing endogenous RNA) networks play important regulatory roles in cancers. In this study, we aimed to construct and verify the ceRNA regulatory network in the occurrence of COE-induced lung squamous cell carcinoma (LUSC). We performed RNA sequencing with lung bronchial epithelial cell (16HBE) and COE induced malignant transformed cell (Rf). Furthermore, we analyzed RNA sequencing data of LUSC and adjacent tissues in the cancer genome atlas (TCGA) database. Combined our data and TCGA data to determine the differentially expressed lncRNAs, miRNAs, mRNAs. lncBASE, miRDB and miRTarBase were used to predict the binding relationship between lncRNA and miRNA, miRNA and mRNA. Based on these, we construct the ceRNA network. FREMSA, dual-luciferase reporter assay, quantitative real-time PCR (qRT-PCR), western-blot were used to verify the regulatory axis. CCK8 assay, phalloidin staining, p53 detection were used to explore the roles of this axis in the COE induced malignant transformation. Results showed 7 lncRNAs, 7 miRNAs and 146 mRNAs were identified. Among these, we constructed a ceRNA network including 1 lncRNA, 2 miRNAs and 9 mRNAs. Further verification confirmed the trend of lncRNA H19, miR-29a-3p and COL1A1 were consistent with sequencing results. H19 and COL1A1 were significantly higher in Rf than in 16HBE and miR-29a-3p was reverse. Regulatory investigation revealed H19 increased COL1A1 expression by sponging miR-29a-3p. Knockdown of H19, COL1A1 or overexpression of miR-29a-3p in Rf cells could inhibit cell proliferation, increased cell adhesion and p53 level. However, knockdown of H19 while suppressing the miR-29a-3p partially rescue the malignant phenotype of Rf caused by H19. In conclusion, all these indicated H19 functioned as a ceRNA to increase COL1A1 by sponging miR-29a-3p and promoted COE-induced cell malignant transformation.
    Keywords bioluminescence assay ; cell adhesion ; cell proliferation ; databases ; epithelial cells ; genome ; lung neoplasms ; lungs ; microRNA ; ovens ; phalloidine ; phenotype ; pollution ; quantitative polymerase chain reaction ; squamous cell carcinoma
    Language English
    Dates of publication 2022-1115
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 280652-6
    ISSN 1873-6424 ; 0013-9327 ; 0269-7491
    ISSN (online) 1873-6424
    ISSN 0013-9327 ; 0269-7491
    DOI 10.1016/j.envpol.2022.120194
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4136: Show issues

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