Article ; Online: Programmable low-cost DNA-based platform for viral RNA detection.
2020 Volume 6, Issue 39
Abstract: ... for low-cost and rapid detection of viral RNA with DNA nanoswitches that mechanically reconfigure ... RNA with selective and multiplexed detection between related viruses and viral strains. For clinical ... adaptable to other viruses, as demonstrated by quickly developing DNA nanoswitches to detect SARS-CoV-2 RNA ...
| Abstract | Detection of viruses is critical for controlling disease spread. Recent emerging viral threats, including Zika virus, Ebola virus, and SARS-CoV-2 responsible for coronavirus disease 2019 (COVID-19) highlight the cost and difficulty in responding rapidly. To address these challenges, we develop a platform for low-cost and rapid detection of viral RNA with DNA nanoswitches that mechanically reconfigure in response to specific viruses. Using Zika virus as a model system, we show nonenzymatic detection of viral RNA with selective and multiplexed detection between related viruses and viral strains. For clinical-level sensitivity in biological fluids, we paired the assay with sample preparation using either RNA extraction or isothermal preamplification. Our assay requires minimal laboratory infrastructure and is adaptable to other viruses, as demonstrated by quickly developing DNA nanoswitches to detect SARS-CoV-2 RNA in saliva. Further development and field implementation will improve our ability to detect emergent viral threats and ultimately limit their impact. |
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| MeSH term(s) | Base Sequence ; Betacoronavirus/genetics ; COVID-19 ; Cell Line, Tumor ; Coronavirus Infections/diagnosis ; Coronavirus Infections/virology ; DNA, Single-Stranded/genetics ; Dengue/diagnosis ; Dengue/virology ; Dengue Virus/genetics ; Electrophoresis, Agar Gel/economics ; Electrophoresis, Agar Gel/methods ; Humans ; Limit of Detection ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/virology ; RNA, Viral/genetics ; SARS-CoV-2 ; Saliva/virology ; Sequence Analysis, RNA/economics ; Sequence Analysis, RNA/methods ; Zika Virus/genetics ; Zika Virus Infection/diagnosis ; Zika Virus Infection/virology |
| Chemical Substances | DNA, Single-Stranded ; RNA, Viral |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-09-25 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural |
| ZDB-ID | 2810933-8 |
| ISSN | 2375-2548 ; 2375-2548 |
| ISSN (online) | 2375-2548 |
| ISSN | 2375-2548 |
| DOI | 10.1126/sciadv.abc6246 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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