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  1. Article: Effects of a Shuangling Fuzheng anticancer preparation on the proliferation of SGC-7901 cells and immune function in a cyclophosphamide-treated murine model.

    Chen, Hua-Sheng / Chen, Jue / Cui, De-Li / Zheng, Yuan-Yuan / Xu, Ai-Hua / Chen, Gang / Jia, Ling-Chang

    World journal of gastroenterology

    2007  Volume 13, Issue 48, Page(s) 6575–6580

    Abstract: Aim: To study the inhibitory effects of a Shuangling Fuzheng anticancer preparation (SFAP ...

    Abstract Aim: To study the inhibitory effects of a Shuangling Fuzheng anticancer preparation (SFAP) on the human gastric cancer cell line SGC-7901 in vitro as well as its immune-modulated effects in a cyclophosphamide-treated murine model.
    Methods: MTT experiments and immunocytochemistry ABC experiments were performed for detecting the proliferation of SGC-7901 cells in vitro and protein expression of c-myc. The staphylococcal protein A (SPA) rosette test was utilized for measuring the ratio of T-lymphocyte subsets from peripheral blood in a cyclophosphamide-treated murine model. Enzyme-linked immunosorbant assay (ELISA) was performed for measuring the levels of serum sIL-2R in treated mice, while immunoturbidimetry was used for measuring the levels of immunoglobulins (Ig).
    Results: SFAP (40-640 mg/L, 48 h) inhibited the proliferation of SGC-7901 cells, and a positive correlation was noted between inhibitory effects and dosage. At a dosage of 160-320 mg/L in cultured cells, the expression of c-myc was decreased. SFAP (50-200 mg/kg) increased the percentage of CD3+ and CD4+ T-lymphocytes, the ratio of CD4/CD8, and the contents of Ig such as IgM, IgG or IgA, but decreased the levels of serum sIL-2R in peripheral blood from cyclophosphamide-treated mice.
    Conclusion: SFAP can inhibit the proliferation of SGC-7901 cells via the c-myc gene. In addition, SFAP can modulat the cellular and humoral immunity in cyclophosphamide-induced immunosuppressed mice.
    MeSH term(s) Animals ; Antibody Formation/drug effects ; Antineoplastic Agents, Phytogenic/pharmacology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cells, Cultured ; Cyclophosphamide/pharmacology ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal/pharmacology ; Female ; Humans ; Immunity, Cellular/drug effects ; Immunoglobulins/blood ; Immunosuppression ; Immunosuppressive Agents/pharmacology ; Mice ; Mice, Inbred ICR ; Proto-Oncogene Proteins c-myc/metabolism ; Receptors, Interleukin-2/blood ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/pathology ; T-Lymphocyte Subsets/metabolism ; T-Lymphocyte Subsets/pathology
    Chemical Substances Antineoplastic Agents, Phytogenic ; Drugs, Chinese Herbal ; Immunoglobulins ; Immunosuppressive Agents ; MYC protein, human ; Proto-Oncogene Proteins c-myc ; Receptors, Interleukin-2 ; Shuangling Fuzheng anticancer preparation ; Cyclophosphamide (8N3DW7272P)
    Language English
    Publishing date 2007-05-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2185929-2
    ISSN 2219-2840 ; 1007-9327
    ISSN (online) 2219-2840
    ISSN 1007-9327
    DOI 10.3748/wjg.v13.i48.6575
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Study of Shuangling Fuzheng anti-tumor preparation on proliferation and c-myc gene expression of SGC-7901 cells].

    Chen, Hua-Sheng / Xu, Ai-Hua / Jia, Lin-Chang / Ren, Li

    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

    2006  Volume 31, Issue 21, Page(s) 1822–1824

    Abstract: Objective: To study the effect of Shuangling Fuzheng anti-tumor preparation (SLAP) five groups ...

    Abstract Objective: To study the effect of Shuangling Fuzheng anti-tumor preparation (SLAP) five groups on proliferation and c-myc gene expression of SGC-7901 cells in vitro.
    Method: The inhibitory effect of single SLAP (40 -640 microg x mL(-1)) and combined therapy with adriamycin (0.4, 4.0 microg x mL(-1) or cisplatin (0.1,1.0 microg x mL(-1) on human gastric carcinoma SGC-7901 cells proliferation were observed by MTT colorimetric analysis method. Technique of flow cytometry in vitro was used to measure the rate of positive sign of SLAP (80 - 320 microg x mL(-1)) on c-myc gene protein of SGC-7901 cells.
    Result: SGC-7901 cells proliferation were inhibited by single SLAP in dose of 40 - 640 microg x mL(-1) 24 h. Its inhibitory rate was increased with increase of dose. The inhibitory rate on SGC-7901 cells could be increased by SLAP in dose of 40 - 640 microg x mL(-1) plus adriamycin in dose of 0.4 and 4.0 microg x mL(-1) or plus cisplatin in dose of 0.1 and 1.0 microg x mL(-1). At the same time, SLAP (80 - 320 microg x mL(-1)) also could inhibite the expression of c-myc gene of SGC -7901 cells.
    Conclusion: Single SLAP had inhibiting effect on human gastric carcinoma cells proliferation with a dose-effect relationship and synergic effect while combined with adriamycin or cisplatin. To inhibit the expression of c-myc gene of human gastric carcinoma cells might be one of action mechanisms of SLAP, which inhibited tumor cells proliferation.
    MeSH term(s) Antibiotics, Antineoplastic/pharmacology ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents, Phytogenic/administration & dosage ; Antineoplastic Agents, Phytogenic/isolation & purification ; Antineoplastic Agents, Phytogenic/pharmacology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cisplatin/pharmacology ; Dose-Response Relationship, Drug ; Doxorubicin/pharmacology ; Drug Combinations ; Drug Synergism ; Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/isolation & purification ; Drugs, Chinese Herbal/pharmacology ; Gene Expression Regulation, Neoplastic/drug effects ; Genes, myc ; Humans ; Plants, Medicinal/chemistry ; Proto-Oncogene Proteins c-myc/metabolism ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/pathology
    Chemical Substances Antibiotics, Antineoplastic ; Antineoplastic Agents ; Antineoplastic Agents, Phytogenic ; Drug Combinations ; Drugs, Chinese Herbal ; Proto-Oncogene Proteins c-myc ; Doxorubicin (80168379AG) ; Cisplatin (Q20Q21Q62J)
    Language Chinese
    Publishing date 2006-11
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1004649-5
    ISSN 1001-5302 ; 0254-0029
    ISSN 1001-5302 ; 0254-0029
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Remote Manipulation of TRPV1 Signaling by Near-Infrared Light-Triggered Nitric Oxide Nanogenerators for Specific Cancer Therapy.

    Wang, Shuangling / Wang, Yalin / Lv, Jie / Xu, Chunzhe / Wei, Yuxin / Wang, Guiying / Li, Meng

    Advanced healthcare materials

    2024  Volume 13, Issue 10, Page(s) e2303579

    Abstract: Specific activation of transient receptor potential vanilloid member 1 (TRPV1) channels provides a new avenue for cancer treatment by inducing excessive ... ...

    Abstract Specific activation of transient receptor potential vanilloid member 1 (TRPV1) channels provides a new avenue for cancer treatment by inducing excessive Ca
    MeSH term(s) Antineoplastic Agents/pharmacology ; Infrared Rays ; Nanoparticles ; Neoplasms/drug therapy ; Nitric Oxide/metabolism ; Phototherapy ; Humans
    Chemical Substances Antineoplastic Agents ; Nitric Oxide (31C4KY9ESH)
    Language English
    Publishing date 2024-01-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2649576-4
    ISSN 2192-2659 ; 2192-2640
    ISSN (online) 2192-2659
    ISSN 2192-2640
    DOI 10.1002/adhm.202303579
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: VEGF mimetic peptide-conjugated nanoparticles for magnetic resonance imaging and therapy of myocardial infarction.

    Li, Bing / Li, Yingxu / Chen, Shuangling / Wang, Yuji / Zheng, Yuanyuan

    Journal of controlled release : official journal of the Controlled Release Society

    2023  Volume 360, Page(s) 44–56

    Abstract: To reduce the mortality of myocardial infarction (MI), accurate detection of the infarct and appropriate prevention against ischemia/reperfusion (I/R) induced cardiac dysfunction are highly desired. Considering that vascular endothelial growth factor ( ... ...

    Abstract To reduce the mortality of myocardial infarction (MI), accurate detection of the infarct and appropriate prevention against ischemia/reperfusion (I/R) induced cardiac dysfunction are highly desired. Considering that vascular endothelial growth factor (VEGF) receptors are overexpressed in the infarcted heart and VEGF mimetic peptide QK binds specifically to VEGF receptors and activates vascularization, the PEG-QK-modified, gadolinium-doped carbon dots (GCD-PEG-QK) were formulated. This research aims to investigate the magnetic resonance imaging (MRI) capability of GCD-PEG-QK on myocardial infarct and their therapeutic effect on I/R-induced myocardial injury. These multifunctional nanoparticles exhibited good colloidal stability, excellent fluorescent and magnetic property, and satisfactory biocompatibility. Intravenous injection of GCD-PEG-QK nanoparticles post myocardial I/R displayed accurate MRI of the infarct, enhanced efficacy of QK peptide on pro-angiogenesis, and amelioration of cardiac fibrosis, remodeling and dysfunction, probably via the improvement on QK's in vivo stability and MI-targeting. Collectively, the data suggested that this theranostic nanomedicine can realize precise MRI and effective therapy for acute MI in a non-invasive manner.
    MeSH term(s) Humans ; Vascular Endothelial Growth Factor A/pharmacology ; Peptides/therapeutic use ; Peptides/chemistry ; Myocardial Infarction/diagnostic imaging ; Myocardial Infarction/drug therapy ; Magnetic Resonance Imaging ; Nanoparticles
    Chemical Substances Vascular Endothelial Growth Factor A ; Peptides
    Language English
    Publishing date 2023-06-20
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2023.06.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Detection of

    Xie, Min / Shao, Jin / Wan, Zhe / Yan, Ting / Zhu, Sainan / Li, Shuangling / Yu, Jin

    Frontiers in microbiology

    2023  Volume 13, Page(s) 1070688

    Abstract: Background: Intra-abdominal candidiasis (IAC) is the predominant type of invasive candidiasis with high mortality in critically ill patients. This study aimed to investigate whether the polymerase chain reaction (PCR) assay for detecting : Methods: A ...

    Abstract Background: Intra-abdominal candidiasis (IAC) is the predominant type of invasive candidiasis with high mortality in critically ill patients. This study aimed to investigate whether the polymerase chain reaction (PCR) assay for detecting
    Methods: A prospective single-center cohort study of surgical patients at high risk for IAC was conducted in the ICU. PF was collected from the abdominal drainage tubes (within 24 h) or by percutaneous puncture. Direct PF smear microscopy, PF culture, blood culture, and serum (1-3)-β-D-glucan were performed in all patients. For
    Results: Among 83 patients at high risk for IAC, the IAC criteria were present in 17 (20.5%). The sensitivity and specificity of the
    Conclusion: Detection of
    Language English
    Publishing date 2023-01-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.1070688
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: [Related factors of viral nucleic acid change in critically ill patients with SARS-CoV-2 infection after treatment with Nirmatrelvir/Ritonavir: a single center retrospective cohort study].

    Yan, Miao / Xie, Min / Zhu, Sainan / Li, Haixia / Li, Shuangling

    Zhonghua wei zhong bing ji jiu yi xue

    2023  Volume 35, Issue 11, Page(s) 1150–1156

    Abstract: Objective: To describe negative conversion and rebound of patients with severe and critical acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection after treatment with Nirmatrelvir/Ritonavir, and to analyze related factors associating with ... ...

    Abstract Objective: To describe negative conversion and rebound of patients with severe and critical acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection after treatment with Nirmatrelvir/Ritonavir, and to analyze related factors associating with failure of SARS-CoV-2 negative conversion and relapse and prognosis.
    Methods: A single center retrospective cohort study was conducted. Patients aged ≥ 16 years old who were diagnosed with severe or critical SARS-CoV-2 infection and took Nirmatrelvir/Ritonavir for 5 days in Peking University First Hospital from December 7, 2022 to January 27, 2023, were included. General characteristics and clinical data were collected from electronic medical record system. The Kaplan-Meier curve of SARS-CoV-2 negative conversion was drawn. Factors with P < 0.10 were incorporated into multivariate Logistic regression model to analyze the relationship between the factors and persistent nucleic acid positive and rebound.
    Results: A total of 31 severe and 37 critical SARS-CoV-2 infection patients were included. The median duration from initiation of Nirmatrelvir/Ritonavir to negative conversion of SARS-CoV-2 for both was 6.0 days, and the negative conversion rate on day 15 was 93.5% and 86.5%, respectively. SARS-CoV-2 rebound was observed in 7 patients (11.3%), among whom were 1 severe patient and 6 critical patients. The above 7 patients with SARS-CoV-2 rebound and 6 patients with failure of SARS-CoV-2 negative conversion were compared with 55 patients with persistent negative conversion. Factors with P < 0.10, including the lowest lymphocyte count (LYM), the highest D-dimer, the highest procalcitonin (PCT), the lowest Ct value, cardiovascular diseases other than hypertension and coronary heart disease, were incorporated into multivariate Logistic regression analysis. The decreased LYM [odds ratio (OR) = 0.146, 95% confidence interval (95%CI) was 0.031-0.689, P = 0.015] and the increased PCT (OR = 2.008, 95%CI was 1.042-3.868, P = 0.037) were revealed to be independent risk factors of the failure of SARS-CoV-2 negative conversion or rebound. The proportion of mechanical ventilation and invasive ventilation were significantly higher in patients with persistent SARS-CoV-2 infection or rebound than those in patients with SARS-CoV-2 negative conversion (84.6% vs. 38.2%, 69.2% vs. 25.5%, both P < 0.01), but no significant difference in mechanical ventilation and invasive ventilation duration was observed. Compared with the patients with SARS-CoV-2 negative conversion, more patients with persistent SARS-CoV-2 infection or rebound were admitted to intensive care unit (ICU, 76.9% vs. 50.9%), and length of ICU stay in patients with persistent SARS-CoV-2 infection or rebound tended to be longer [days: 13.0 (10.3, 24.3) vs. 11.0 (5.3, 23.0), P > 0.05].
    Conclusions: The decreased LYM and increased PCT are independent risk factors for the failure of SARS-CoV-2 negative conversion or rebound in patients with severe and critical SARS-CoV-2 infection. Attention should be paid to these patients for their poor prognosis.
    MeSH term(s) Humans ; Adolescent ; COVID-19 ; SARS-CoV-2 ; Retrospective Studies ; Ritonavir/therapeutic use ; Critical Illness ; COVID-19 Drug Treatment
    Chemical Substances nirmatrelvir (7R9A5P7H32) ; Ritonavir (O3J8G9O825)
    Language Chinese
    Publishing date 2023-11-20
    Publishing country China
    Document type English Abstract ; Journal Article
    ISSN 2095-4352
    ISSN 2095-4352
    DOI 10.3760/cma.j.cn121430-20230718-00523
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Tumor microenvironment-responsive artesunate loaded Z-scheme heterostructures for synergistic photo-chemodynamic therapy of hypoxic tumor.

    Lv, Jie / Wang, Xiaoyu / Zhang, Xue / Xu, Runpei / Hu, Shuyang / Wang, Shuangling / Li, Meng

    Asian journal of pharmaceutical sciences

    2023  Volume 18, Issue 3, Page(s) 100798

    Abstract: Tumor microenvironment (TME) with the particular features of severe hypoxia, insufficient endogenous ... ...

    Abstract Tumor microenvironment (TME) with the particular features of severe hypoxia, insufficient endogenous H
    Language English
    Publishing date 2023-03-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2650931-3
    ISSN 2221-285X ; 1818-0876 ; 2221-285X
    ISSN (online) 2221-285X
    ISSN 1818-0876 ; 2221-285X
    DOI 10.1016/j.ajps.2023.100798
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  8. Article ; Online: The complete chloroplast genome of

    Shi, Shuo / Li, Shuangling / Zhang, Shijia / Shen, Fengjiao / Niu, Jingyuan / Li, Lin / Zhao, Jiancheng

    Mitochondrial DNA. Part B, Resources

    2021  Volume 6, Issue 3, Page(s) 837–839

    Abstract: ... ...

    Abstract Mnium
    Language English
    Publishing date 2021-03-11
    Publishing country England
    Document type Journal Article
    ISSN 2380-2359
    ISSN (online) 2380-2359
    DOI 10.1080/23802359.2021.1884025
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  9. Article ; Online: The complete chloroplast genome of

    Li, Shuangling / Shen, Fengjiao / Zhang, Shijia / Niu, Jingyuan / Niu, Yulu / Li, Lin / Zhao, Jiancheng

    Mitochondrial DNA. Part B, Resources

    2021  Volume 6, Issue 9, Page(s) 2632–2634

    Abstract: Rhodobryum ... ...

    Abstract Rhodobryum laxelimbatum
    Language English
    Publishing date 2021-08-13
    Publishing country England
    Document type Journal Article
    ISSN 2380-2359
    ISSN (online) 2380-2359
    DOI 10.1080/23802359.2021.1962759
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Effects of heated-treating temperature on the stability and electrochemical performance of alginate-based multi-crosslinked biomembranes.

    Wang, Bin / Fang, Han / Han, Xing / Li, Xiaojun / Sheng, Jinyue / Wang, Minghui / Cui, Wei / Zhong, Shuangling / Zhang, Zhidan / Cui, Xuejun

    International journal of biological macromolecules

    2024  Volume 263, Issue Pt 2, Page(s) 130350

    Abstract: In this study, the organosilane nanoparticles as additive and crosslinker were prepared and incorporated into sodium alginate to fabricate a series of alginate-based multi-crosslinked biomembranes at different thermal treatment temperature without the ... ...

    Abstract In this study, the organosilane nanoparticles as additive and crosslinker were prepared and incorporated into sodium alginate to fabricate a series of alginate-based multi-crosslinked biomembranes at different thermal treatment temperature without the usage of another crosslinking agent. The effects of treatment temperature on the stability of biomembranes including dimensional, oxidative, hydrolytic and mechanical stability were investigated in detail. As a whole, the stability of biomembranes exhibited increasing tendency with the increment of treatment temperature due to the formation of more compact internal network structure. The electrochemical performance of biomembranes in respect to their potential as proton exchange membranes for direct methanol fuel cell application were also investigated based on the treatment temperature. The results revealed that the biomembranes possessed excellent methanol resistance and the methanol diffusion coefficient decreased with the increment of treatment temperature. The biomembrane with 120 °C heat-treatment showed the optimal selectivity (14.30 × 10
    MeSH term(s) Hot Temperature ; Temperature ; Methanol ; Alginates ; Fluorocarbon Polymers
    Chemical Substances perfluorosulfonic acid (39464-59-0) ; Methanol (Y4S76JWI15) ; Alginates ; Fluorocarbon Polymers
    Language English
    Publishing date 2024-02-24
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.130350
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