Article: Effects of a Shuangling Fuzheng anticancer preparation on the proliferation of SGC-7901 cells and immune function in a cyclophosphamide-treated murine model.
World journal of gastroenterology
2007 Volume 13, Issue 48, Page(s) 6575–6580
Abstract: Aim: To study the inhibitory effects of a Shuangling Fuzheng anticancer preparation (SFAP ...
Abstract | Aim: To study the inhibitory effects of a Shuangling Fuzheng anticancer preparation (SFAP) on the human gastric cancer cell line SGC-7901 in vitro as well as its immune-modulated effects in a cyclophosphamide-treated murine model. Methods: MTT experiments and immunocytochemistry ABC experiments were performed for detecting the proliferation of SGC-7901 cells in vitro and protein expression of c-myc. The staphylococcal protein A (SPA) rosette test was utilized for measuring the ratio of T-lymphocyte subsets from peripheral blood in a cyclophosphamide-treated murine model. Enzyme-linked immunosorbant assay (ELISA) was performed for measuring the levels of serum sIL-2R in treated mice, while immunoturbidimetry was used for measuring the levels of immunoglobulins (Ig). Results: SFAP (40-640 mg/L, 48 h) inhibited the proliferation of SGC-7901 cells, and a positive correlation was noted between inhibitory effects and dosage. At a dosage of 160-320 mg/L in cultured cells, the expression of c-myc was decreased. SFAP (50-200 mg/kg) increased the percentage of CD3+ and CD4+ T-lymphocytes, the ratio of CD4/CD8, and the contents of Ig such as IgM, IgG or IgA, but decreased the levels of serum sIL-2R in peripheral blood from cyclophosphamide-treated mice. Conclusion: SFAP can inhibit the proliferation of SGC-7901 cells via the c-myc gene. In addition, SFAP can modulat the cellular and humoral immunity in cyclophosphamide-induced immunosuppressed mice. |
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MeSH term(s) | Animals ; Antibody Formation/drug effects ; Antineoplastic Agents, Phytogenic/pharmacology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cells, Cultured ; Cyclophosphamide/pharmacology ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal/pharmacology ; Female ; Humans ; Immunity, Cellular/drug effects ; Immunoglobulins/blood ; Immunosuppression ; Immunosuppressive Agents/pharmacology ; Mice ; Mice, Inbred ICR ; Proto-Oncogene Proteins c-myc/metabolism ; Receptors, Interleukin-2/blood ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/pathology ; T-Lymphocyte Subsets/metabolism ; T-Lymphocyte Subsets/pathology |
Chemical Substances | Antineoplastic Agents, Phytogenic ; Drugs, Chinese Herbal ; Immunoglobulins ; Immunosuppressive Agents ; MYC protein, human ; Proto-Oncogene Proteins c-myc ; Receptors, Interleukin-2 ; Shuangling Fuzheng anticancer preparation ; Cyclophosphamide (8N3DW7272P) |
Language | English |
Publishing date | 2007-05-30 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2185929-2 |
ISSN | 2219-2840 ; 1007-9327 |
ISSN (online) | 2219-2840 |
ISSN | 1007-9327 |
DOI | 10.3748/wjg.v13.i48.6575 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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