Article ; Online: Refining internal bilayer structure of bicelles resolved by extended-q small angle X-ray scattering.
Chemistry and physics of lipids
2020 Volume 231, Page(s) 104945
Abstract: ... layer-core-shell discoidal model can be employed to best fit the extended-q small angle X-ray scattering ...
| Abstract | The internal profile across the bilayer reveals important structural information regarding the crystallinity of acyl chains or the positions of encapsulated species. Here, we demonstrate that a simple five-layer-core-shell discoidal model can be employed to best fit the extended-q small angle X-ray scattering (SAXS) data and resolve the bilayer internal structure (with sub-nanometer resolution) of a nanoscale discoidal system comprised of a mixture of long- and short- chain lipids (known as "bicelles"). In contrast to the traditional core-shell discoidal model, the detailed structure in the hydrophobic core such as the methylene and methyl groups can be distinguished via this model. The refined model is validated by the SAXS data of bicelles whose electron scattering length density of the hydrophobic core is adjusted by the addition of a long-chain lipid with a fluorine-end group. The higher resolution of the bilayer internal structure can be employed to advance our understanding of the interaction and conformation of the membrane and associated molecules, such as membrane-associated proteins and locations of entrapped species in the lipid nanoparticles. |
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| MeSH term(s) | Lipid Bilayers/chemistry ; Lipids/chemistry ; Models, Molecular ; Molecular Structure ; Scattering, Small Angle ; X-Ray Diffraction |
| Chemical Substances | Lipid Bilayers ; Lipids |
| Language | English |
| Publishing date | 2020-07-01 |
| Publishing country | Ireland |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. |
| ZDB-ID | 213869-4 |
| ISSN | 1873-2941 ; 0009-3084 |
| ISSN (online) | 1873-2941 |
| ISSN | 0009-3084 |
| DOI | 10.1016/j.chemphyslip.2020.104945 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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