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Article ; Online: Protective effects of Re-yan-ning mixture on

Han, Lizhu / Kou, Jing / Hu, Kunxia / Wang, Yunlan / Tang, Zhishu / Wu, Zhisheng / Song, Xiao

2021 Volume 59, Issue 1, Page(s) 209–221

Abstract: Context: Re-yan-ning mixture (RYNM) is a new national drug approved by China's State ...

Abstract	Context: Re-yan-ning mixture (RYNM) is a new national drug approved by China's State Food and Drug Administration for the treatment of colds, simple pneumonia and acute bronchitis. Objective: To determine the mechanism of action of RYNM in the treatment of bacterial pneumonia. Materials and methods: Using the network pharmacology approach, the multiple components, component candidate targets and multiple therapeutic targets of RYNM were screened and functionally enriched. Also, we established a rat Results: The network pharmacology approach successfully identified 48 bioactive components in RYNM, and 65 potential targets and 138 signal pathways involved in the treatment of Discussion and conclusions: The present study demonstrated the protective effects of RYNM on
MeSH term(s)	Animals ; Disease Models, Animal ; Drugs, Chinese Herbal/pharmacology ; Inflammation/drug therapy ; Inflammation/microbiology ; Male ; NF-kappa B/metabolism ; Pneumonia, Pneumococcal/microbiology ; Pneumonia, Pneumococcal/prevention & control ; Rats ; Rats, Sprague-Dawley ; Signal Transduction/drug effects ; Streptococcus pneumoniae/drug effects ; Tumor Necrosis Factor-alpha/metabolism
Chemical Substances	Drugs, Chinese Herbal ; NF-kappa B ; Tumor Necrosis Factor-alpha
Language	English
Publishing date	2021-03-07
Publishing country	England
Document type	Journal Article
ZDB-ID	1440131-9
ISSN	1744-5116 ; 1388-0209
ISSN (online)	1744-5116
ISSN	1388-0209
DOI	10.1080/13880209.2021.1872653
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Article ; Online: Systematic review of randomized controlled trials on Gong Liu Ning as a complimentary therapy in the treatment of uterine fibroids

Lu Fan / Jiahui Hu / Mei Han / Guoyan Yang / Shibo Cong / Min Xiong / Ruiting Wang / Gansheng Zhong

Journal of Traditional Chinese Medical Sciences, Vol 10, Iss 4, Pp 415-

2023 Volume 426

Abstract: Objective: To systematically evaluate the efficacy and safety of Gong Liu Ning as a complimentary ... therapy in treatment for uterine fibroids. Methods: Randomized controlled trials on Gong Liu Ning ... with 1907 patients were analyzed. The meta-analysis results of 15 studies showed that Gong Liu Ning combined ...

Abstract	Objective: To systematically evaluate the efficacy and safety of Gong Liu Ning as a complimentary therapy in treatment for uterine fibroids. Methods: Randomized controlled trials on Gong Liu Ning for uterine fibroids from China National Knowledge Infrastructure, Wanfang, China Science and Technology Journal Database, PubMed, Cochrane Library, Embase, Web of Science, Chinese Clinical Trial Registry, and ClinicalTrials.gov were retrieved from inception to March 20, 2023. The risk of bias tool was used to evaluate the quality of the selected studies after they met inclusion and exclusion criteria. The GRADE tool was used to evaluate the quality of evidence, and the RevMan5.3 software was used for data analysis. Results: Twenty studies with 1907 patients were analyzed. The meta-analysis results of 15 studies showed that Gong Liu Ning combined with mifepristone was more efficacious than those observed in controls taking only Western medicine (risk ratio [RR] = 1.22, 95% confidence interval [CI] = [1.11, 1.33], P < .001). Owing to the large heterogeneity, a random-effects model was used to merge the included studies. Gong Liu Ning combined with mifepristone was better in reducing the volume of uterine fibroids (mean difference [MD] = −4.09 cm3, 95%CI [−5.16, −3.02], P < .001), uterine volume (MD = −23.22 cm3, 95%CI [−29.10, −17.33], P < .001), and the levels of female hormones, including progesterone, estrogen, follicle-stimulating hormone, and luteinizing hormone (all P < .001). Ten studies reported adverse events. The methodological quality of the included studies was not high, and a moderate risk of bias was observed. Conclusion: Compared with conventional Western medicine alone, the combined use of Gong Liu Ning has certain advantages in improving efficacy. It shows a certain advanced tendency to reduce the volume of uterine fibroids, uterine volume, and female hormones; however, more high-quality and rigorously designed studies are required for validation.
Keywords	Gong Liu Ning ; Uterine fibroids ; Chinese patent medicine ; Meta-analysis ; Systematic review ; Miscellaneous systems and treatments ; RZ409.7-999
Subject code	610
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Tang Luo Ning, a Traditional Chinese Compound Prescription, Ameliorates Schwannopathy of Diabetic Peripheral Neuropathy Rats by Regulating Mitochondrial Dynamics

Zhu, Jiayue / Yang, Xinwei / Li, Xiao / Han, Shuo / Zhu, Yanbo / Xu, Liping

Frontiers in pharmacology

2021 Volume 12, Page(s) 650448

Abstract: Tang Luo Ning (TLN), a traditional Chinese compound prescription, has been used clinically to treat ...

Abstract	Tang Luo Ning (TLN), a traditional Chinese compound prescription, has been used clinically to treat diabetic peripheral neuropathy (DPN) in China. However, the exact mechanisms remain unclear. The objective of this study is to unravel the effects of TLN on mitochondrial dynamics of DPN in streptozotocin-induced rat models and Schwann cells cultured in 150 mM glucose. Mitochondrial function was determined by Ca
Language	English
Publishing date	2021-05-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.650448
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Article ; Online: Re-Du-Ning injection ameliorates radiation-induced pneumonitis and fibrosis by inhibiting AIM2 inflammasome and epithelial-mesenchymal transition.

Yang, Chenxi / Song, Chenglin / Wang, Yi / Zhou, Wencheng / Zheng, Wei / Zhou, Han / Deng, Guoliang / Li, Haibo / Xiao, Wei / Yang, Zhongqi / Kong, Lingdong / Ge, Huiming / Song, Yaohong / Sun, Yang

Phytomedicine : international journal of phytotherapy and phytopharmacology

2022 Volume 102, Page(s) 154184

Abstract: ... patients, and there is no good medicine to treat it. Re-Du-Ning (RDN) injection is ...

Abstract	Background: Radiation-induced lung injury (RILI) is a common side effect in chest radiotherapy patients, and there is no good medicine to treat it. Re-Du-Ning (RDN) injection is a traditional Chinese medicine that is clinically used to treat upper respiratory tract infections and acute bronchitis. RDN has the advantage of high safety and mild side effects. The mechanism of most traditional Chinese medicine preparations is unknown. Purpose: To illustrate the mechanisms of RDN for the treatment of RILI. Methods: Female C57BL/6 mice were used to establish a RILI model via irradiation, and RDN injection was intraperitoneally administered at doses of 5, 10, and 20 ml/kg. The cytokines were measured by ELISA and qPCR. The data related to Absent in melanoma 2 (AIM2) inflammasome were analyzed via ELISA and a network pharmacological approach. In addition, the data related to epithelial-mesenchymal transition (EMT) were analyzed via immunofluorescence, Western blotting, and a network pharmacological approach. Results: RDN robustly alleviated RILI. Meanwhile, RDN downregulated inflammatory cells' infiltration and the expression of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α. Next, the potential molecular mechanisms of RDN were predicted through network pharmacology analysis. RDN may ameliorate radiation pneumonitis (RP) by inhibiting AIM2-mediated pyroptosis. Moreover, RDN treatment inhibited EMT and phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) pathway. The active compounds from Lonicera japonica Thunb. decreased the phosphorylation of Akt. Conclusion: These findings demonstrate that RDN, as a traditional Chinese medicine preparation, will be a candidate drug for treating RILI.
MeSH term(s)	Animals ; Cytokines ; DNA-Binding Proteins ; Epithelial-Mesenchymal Transition ; Female ; Fibrosis ; Humans ; Inflammasomes ; Lung Injury ; Melanoma ; Mice ; Mice, Inbred C57BL ; Pneumonia ; Proto-Oncogene Proteins c-akt/metabolism ; Radiation Injuries/drug therapy ; Radiation Pneumonitis/drug therapy
Chemical Substances	AIM2 protein, human ; Aim2 protein, mouse ; Cytokines ; DNA-Binding Proteins ; Inflammasomes ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
Language	English
Publishing date	2022-05-21
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2022.154184
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Article: Corrigendum: Tang Luo Ning, a Traditional Chinese Compound Prescription, Ameliorates Schwannopathy of Diabetic Peripheral Neuropathy Rats by Regulating Mitochondrial Dynamics In Vivo and In Vitro.

Zhu, Jiayue / Yang, Xinwei / Li, Xiao / Han, Shuo / Zhu, Yanbo / Xu, Liping

Frontiers in pharmacology

2021 Volume 12, Page(s) 718452

Abstract: This corrects the article DOI: 10.3389/fphar.2021.650448.]. ...

Abstract	[This corrects the article DOI: 10.3389/fphar.2021.650448.].
Language	English
Publishing date	2021-07-22
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.718452
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Article ; Online: Origin and evolution of emerging Liao ning Virus (genus Seadornavirus, family Reoviridae).

Zhang, Jun / Liu, Hong / Wang, Jiahui / Wang, Jiheng / Zhang, Jianming / Wang, Jiayue / Zhang, Xin / Ji, Hongfang / Ding, Zhongfeng / Xia, Han / Zhang, Chunyang / Zhao, Qian / Liang, Guodong

Virology journal

2020 Volume 17, Issue 1, Page(s) 105

Abstract: Background: Liao ning virus (LNV) is a member of the genus Seadornavirus, family Reoviridae and ...

Abstract	Background: Liao ning virus (LNV) is a member of the genus Seadornavirus, family Reoviridae and has been isolated from kinds of vectors in Asia and Australia. However, there are no systematic studies describe the molecular genetic evolution and migration of LNVs. With the development of bioinformatics, viral genetic data combining the information of virus isolation time and locations could be integrated to infer the virus evolution and spread in nature. Methods: Here, a phylogenetic and phylogeographic analysis using Bayesian Markov chain Monte Carlo simulations was conducted on the LNVs isolated from a variety of vectors during 1990-2014 to identify the evolution and migration patterns of LNVs. Results: The results demonstrated that the LNV could be divided into 3 genotypes, of which genotype 1 mainly composed of LNVs isolated from Australia during 1990 to 2014 and the original LNV strain (LNV-NE97-31) isolated from Liaoning province in northern China in 1997, genotype 2 comprised of the isolates all from Xinjiang province in western China and genotype 3 consisted the isolates from Qinghai and Shanxi province of central China. LNVs emerged about 272 years ago and gradually evolved into three lineages in the order genotype 1, genotype 2 and genotype 3. Following phylogeographic analysis, it shows genotype 1 LNVs transmitted from Australia (113°E-153°E,10°S-42°S) to Liaoning province (118°E-125°E,38°N-43°N) in Northeast Asian continent then further spread across the central part of China to western China (75°E-95°E,35°N-50°N). Conclusion: LNVs were initially isolated from Liaoning province of China in the Northeast Asia, however, the present study revealed that LNVs were first appeared in Australia in the South Pacific region and transmitted to mainland China then rapidly spread across China and evolved three different genotypes. The above results suggested that LNV had the characteristics of long-distance transmission and there were great genetic diversity existed in the LNV population. Notably, current information of 80 strains of LNVs are limited. It is of great importance to strengthen the surveillance of LNVs to explore its real origin in nature and monitoring of the LNVs' population variation and maintain vigilance to avoid LNV breaking through the species barrier and further clarify its relationship to human and animal infection.
MeSH term(s)	Animals ; Australia ; Bayes Theorem ; China ; Culicidae/classification ; Culicidae/virology ; Evolution, Molecular ; Genotype ; Phylogeny ; Phylogeography ; Reoviridae/classification ; Reoviridae/genetics ; Sequence Analysis, DNA
Keywords	covid19
Language	English
Publishing date	2020-07-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1743-422X
ISSN (online)	1743-422X
DOI	10.1186/s12985-020-01382-2
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Article ; Online: Protective effects of Re-yan-ning mixture on Streptococcus pneumonia in rats based on network pharmacology

Han, Lizhu / Kou, Jing / Hu, Kunxia / Wang, Yunlan / Tang, Zhishu / Wu, Zhisheng / Song, Xiao

Pharmaceutical Biology. 2021 Jan. 1, v. 59, no. 1 p.207-219

2021

Abstract: Re-yan-ning mixture (RYNM) is a new national drug approved by China's State ...

Abstract	Re-yan-ning mixture (RYNM) is a new national drug approved by China's State Food and Drug Administration for the treatment of colds, simple pneumonia and acute bronchitis. To determine the mechanism of action of RYNM in the treatment of bacterial pneumonia. Using the network pharmacology approach, the multiple components, component candidate targets and multiple therapeutic targets of RYNM were screened and functionally enriched. Also, we established a rat Streptococcus pneumonia model to verify the results of network pharmacology enrichment analysis. Forty male SPF Sprague Dawley rats were divided into four groups of 10 rats: control (normal saline), model (normal saline), levofloxacin-intervened and RYNM-intervened groups. IL-10, NOS2, COX-1, IL-6, TNF-α and NF-κB in serum and BALF were detected by ELISA. Western blot detected IL-17, IL-6, TNF-α, COX-2 and Bcl-2. The network pharmacology approach successfully identified 48 bioactive components in RYNM, and 65 potential targets and 138 signal pathways involved in the treatment of Streptococcus pneumonia with RYNM. The in vivo experiments indicated that model group has visible inflammation and lesions while RYNM and levofloxacin groups have not. The RYNM exhibited its therapeutic effects on Streptococcus pneumonia mainly via the regulation of cell proliferation and survival through the IL-6/IL-10/IL-17, Bax/Bcl-2, COX-1/COX-2, NF-κB and TNF-α signalling pathways. The present study demonstrated the protective effects of RYNM on Streptococcus pneumonia, providing a potential mechanism for the treatment of bacterial pneumonia with RYNM.
Keywords	Food and Drug Administration ; Streptococcus ; Western blotting ; bacterial pneumonia ; blood serum ; bronchitis ; cell proliferation ; inflammation ; interleukin-10 ; interleukin-17 ; interleukin-6 ; levofloxacin ; males ; mechanism of action ; models ; pharmacology ; rats ; therapeutics ; China ; Traditional Chinese medicine ; pneumonia ; signal pathway
Language	English
Dates of publication	2021-0101
Size	p. 207-219.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	1440131-9
ISSN	1744-5116 ; 1388-0209
ISSN (online)	1744-5116
ISSN	1388-0209
DOI	10.1080/13880209.2021.1872653
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Protective effects of Re-yan-ning mixture on Streptococcus pneumonia in rats based on network pharmacology

Lizhu Han / Jing Kou / Kunxia Hu / Yunlan Wang / Zhishu Tang / Zhisheng Wu / Xiao Song

Pharmaceutical Biology, Vol 59, Iss 1, Pp 209-

2021 Volume 221

Abstract: Context Re-yan-ning mixture (RYNM) is a new national drug approved by China's State ...

Abstract	Context Re-yan-ning mixture (RYNM) is a new national drug approved by China's State Food and Drug Administration for the treatment of colds, simple pneumonia and acute bronchitis. Objective To determine the mechanism of action of RYNM in the treatment of bacterial pneumonia. Materials and methods Using the network pharmacology approach, the multiple components, component candidate targets and multiple therapeutic targets of RYNM were screened and functionally enriched. Also, we established a rat Streptococcus pneumonia model to verify the results of network pharmacology enrichment analysis. Forty male SPF Sprague Dawley rats were divided into four groups of 10 rats: control (normal saline), model (normal saline), levofloxacin-intervened and RYNM-intervened groups. IL-10, NOS2, COX-1, IL-6, TNF-α and NF-κB in serum and BALF were detected by ELISA. Western blot detected IL-17, IL-6, TNF-α, COX-2 and Bcl-2. Results The network pharmacology approach successfully identified 48 bioactive components in RYNM, and 65 potential targets and 138 signal pathways involved in the treatment of Streptococcus pneumonia with RYNM. The in vivo experiments indicated that model group has visible inflammation and lesions while RYNM and levofloxacin groups have not. The RYNM exhibited its therapeutic effects on Streptococcus pneumonia mainly via the regulation of cell proliferation and survival through the IL-6/IL-10/IL-17, Bax/Bcl-2, COX-1/COX-2, NF-κB and TNF-α signalling pathways. Discussion and conclusions The present study demonstrated the protective effects of RYNM on Streptococcus pneumonia, providing a potential mechanism for the treatment of bacterial pneumonia with RYNM.
Keywords	traditional chinese medicine ; pneumonia ; signal pathway ; Therapeutics. Pharmacology ; RM1-950
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Taylor & Francis Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Re-Du-Ning injection ameliorates radiation-induced pneumonitis and fibrosis by inhibiting AIM2 inflammasome and epithelial-mesenchymal transition

Yang, Chenxi / Song, Chenglin / Wang, Yi / Zhou, Wencheng / Zheng, Wei / Zhou, Han / Deng, Guoliang / Li, Haibo / Xiao, Wei / Yang, Zhongqi / Kong, Lingdong / Ge, Huiming / Song, Yaohong / Sun, Yang

Phytomedicine. 2022 May 17,

2022

Abstract: ... there is no good medicine to treat it. Re-Du-Ning (RDN) injection is a traditional Chinese medicine ...

Abstract	Radiation-induced lung injury (RILI) is a common side effect in chest radiotherapy patients, and there is no good medicine to treat it. Re-Du-Ning (RDN) injection is a traditional Chinese medicine that is clinically used to treat upper respiratory tract infections and acute bronchitis. RDN has the advantage of high safety and mild side effects. The mechanism of most traditional Chinese medicine preparations is unknown. To illustrate the mechanisms of RDN for the treatment of RILI. Female C57BL/6 mice were used to establish a RILI model via irradiation, and RDN injection was intraperitoneally administered at doses of 5, 10, and 20 mL/kg. The cytokines were measured by ELISA and qPCR. The data related to Absent in melanoma 2 (AIM2) inflammasome were analyzed via ELISA and a network pharmacological approach. In addition, the data related to epithelial-mesenchymal transition (EMT) were analyzed via immunofluorescence, Western blotting, and a network pharmacological approach. RDN robustly alleviated RILI. Meanwhile, RDN downregulated inflammatory cells’ infiltration and the expression of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α. Next, the potential molecular mechanisms of RDN were predicted through network pharmacology analysis. RDN may ameliorate radiation pneumonitis by inhibiting AIM2-mediated pyroptosis. Moreover, RDN treatment inhibited EMT and phosphoinositide-3-kinase (PI3K)/protein kinase B (Akt) pathway. The active compounds from Lonicera japonica Thunb. decreased the phosphorylation of Akt. These findings demonstrate that RDN, as a traditional Chinese medicine preparation, will be a candidate drug for treating RILI.
Keywords	Lonicera japonica ; Oriental traditional medicine ; adverse effects ; bronchitis ; chest ; drugs ; females ; fibrosis ; fluorescent antibody technique ; inflammasomes ; interleukin-6 ; irradiation ; lungs ; melanoma ; models ; pharmacology ; phosphatidylinositol 3-kinase ; phosphorylation ; pneumonia ; pyroptosis ; radiotherapy
Language	English
Dates of publication	2022-0517
Publishing place	Elsevier GmbH
Document type	Article
Note	Pre-press version
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2022.154184
Database	NAL-Catalogue (AGRICOLA)

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Article: [Characterization of Dissolved Organic Matter Fractions in the Ning-Meng Section of the Yellow River and Relationship with Metal Ions].

Xi, Yue / Wang, Ting / Ni, Jin-Ren / Han, Peng / Yi, Ma-Lan / Zheng, Tong / Jiang, Yong / Ma, Ruo-Qi / Cui, Feng

Huan jing ke xue= Huanjing kexue

2018 Volume 39, Issue 9, Page(s) 4114–4121

Abstract: ... vary. In this study, seven natural waters, extending from Xiaheyan to Toudaoguai along the Ning-Meng ... analyzed. Results show that DOC gradually increased along an upstream to downstream continuum in the Ning ...

Abstract	Dissolved organic matter(DOM)is an important element of natural aquatic systems. Due to differences in their hydrophobic/hydrophilic properties and various functional groups, chemical appearances of DOM fractions also vary. In this study, seven natural waters, extending from Xiaheyan to Toudaoguai along the Ning-Meng section of the Yellow river, were sampled in April 2015. Four DOM fractions were obtained by pumping through XAD-4 and XAD-8 resins, i.e., hydrophobic acid (HOA), hydrophobic base (HOB), weak hydrophobic acid (WHOA), and hydrophilic matter (HYI). Based on detection by three-dimensional excitation-emission matrix fluorescence (EEM) and correlation analysis, relationships with five metal ions (Pb, Zn, Cu, Cr, As) were analyzed. Results show that DOC gradually increased along an upstream to downstream continuum in the Ning-Meng section. HYI (small molecular proteins) was the main DOM fraction present, followed by HOA, suggesting enhanced microbial-sourced impact from industrial sewage discharges. The significant peaks of humic-like (A, C) and protein-like compounds (T
Language	Chinese
Publishing date	2018-09-06
Publishing country	China
Document type	English Abstract ; Journal Article
ISSN	0250-3301
ISSN	0250-3301
DOI	10.13227/j.hjkx.201712249
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