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Article ; Online: Selection of reference genes for RT-qPCR analysis of rice with Rhizoctonia solani infection and biocontrol PGPR/KSi application.

Hashemipetroudi, Seyyed Hamidreza / Ghorbani, Hamidreza / Rostami, Mehdi / Rezaei, Ali / Goodwin, Paul H / Kuhlmann, Markus

Molecular biology reports

2023 Volume 50, Issue 5, Page(s) 4225–4237

Abstract: Background: Rhizoctonia solani (AG1 IA) is an important pathogen of rice (Oryza sativa L.) that causes rice sheath blight (RSB). Since control of RSB by breeding and fungicides have had limited success, novel strategies like biocontrol with plant growth- ...

Abstract	Background: Rhizoctonia solani (AG1 IA) is an important pathogen of rice (Oryza sativa L.) that causes rice sheath blight (RSB). Since control of RSB by breeding and fungicides have had limited success, novel strategies like biocontrol with plant growth-promoting rhizobacteria (PGPR) can be an effective alternative. Method and results: Seven commonly used reference genes (RGs), 18SrRNA, ACT1, GAPDH2, UBC5, RPS27, eIF4a and CYP28, were evaluated for their stability in rice-R. solani-PGPR interaction for real-time quantitative PCR (RT-qPCR) analysis. Different algorithms were examined, Delta Ct, geNorm, NormFinder, BestKeeper, and comprehensive ranking by RefFinder, to evaluate RT-qPCR of rice in tissues infected with R. solani and treated with the PGPR strains, Pseudomonas saponiphilia and Pseudomonas protegens, with potassium silicate (KSi) alone or in combination with each PGPR strain. RG stability was affected for each treatment and treatment-specific RG selection was suggested. Validation analysis was done for nonexpressor of PR-1(NPR1) for each treatment. Conclusion: Overall, ACT1 was the most stable RG with R. solani infection alone, GAPDH2 with R. solani infection plus KSi, UBC5 with R. solani infection plus P. saponiphilia, and eIF4a with R. solani infection plus P. protegens. Both ACT1 and RPS27 were the most stable with the combination of KSi and P. saponiphilia, while RPS27 was the most stable with the combination of KSi and P. protegens.
MeSH term(s)	Oryza/genetics ; Oryza/microbiology ; Plant Breeding ; Rhizoctonia/genetics ; Plant Development ; Plant Diseases/genetics ; Plant Diseases/microbiology
Language	English
Publishing date	2023-03-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-023-08361-0
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Article ; Online: Does Renal Mass with Venous Thrombosis always Indicate Renal Cell Carcinoma? A Case Series.

Ghorbani, Hamidreza / Farzadnia, Mehdi / Golshan, Alireza

Urology journal

2022 Volume 19, Issue 6, Page(s) 466–471

Abstract: Intravascular tumor extension in the major renal veins or their tributaries, as a rare but important clinical entity that can change the disease stage, prognosis, and approach to treatment. There is limited literature on the obstruction of renal vein and ...

Abstract	Intravascular tumor extension in the major renal veins or their tributaries, as a rare but important clinical entity that can change the disease stage, prognosis, and approach to treatment. There is limited literature on the obstruction of renal vein and IVC by tumor thrombus in other types of renal tumors that are not of RCC type. We presented four different renal tumor cases with the presence of gross renal vein or IVC thrombosis. Although the incidence of renal vein and IVC tumor thrombus might be suggestive of (often diagnosed as) RCC, the possibility of other non-RCC renal tumors should be included in the differential diagnosis.
MeSH term(s)	Humans ; Carcinoma, Renal Cell/complications ; Carcinoma, Renal Cell/diagnosis ; Kidney Neoplasms/complications ; Kidney Neoplasms/diagnosis ; Venous Thrombosis/diagnosis ; Venous Thrombosis/etiology
Language	English
Publishing date	2022-12-06
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2251940-3
ISSN	1735-546X ; 1735-1308
ISSN (online)	1735-546X
ISSN	1735-1308
DOI	10.22037/uj.v19i.7025
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Article: Renal Tumors in Pregnancy: A Case Report Focusing on the Timing of the Surgery and Patient Positioning.

Ghorbani, Hamidreza / Mottaghi, Mahdi / Soltani, Salman

Case reports in obstetrics and gynecology

2022 Volume 2022, Page(s) 1143478

Abstract: Management of renal mass during pregnancy is challenging. There is no consensus regarding the fundamental timing issues (which trimester) of the interventions and patient positioning during the procedures. We present three pregnant women with renal mass ... ...

Abstract	Management of renal mass during pregnancy is challenging. There is no consensus regarding the fundamental timing issues (which trimester) of the interventions and patient positioning during the procedures. We present three pregnant women with renal mass and their management, focusing on patient positioning and timing of surgical intervention. All patients were positioned supine with a 30-degree rotation to the left lateral without signs of compromising fetal circulation. This report's three major takeaway points are the following: (1) Renal mass biopsy might be more beneficial in pregnant women than the normal population (unless CT findings suggest vascular angiomyolipomas) to achieve a definite diagnosis and avoid unnecessary interventions during pregnancy. (2) Surgical interventions, if indicated, should be performed as soon as possible and are applicable in all trimesters of pregnancy. (3) A minimum of 15-degree left lateral tilt (for both right- and left-sided renal masses) can provide enough venous return during the nephrectomy.
Language	English
Publishing date	2022-02-26
Publishing country	United States
Document type	Case Reports
ZDB-ID	2627654-9
ISSN	2090-6692 ; 2090-6684
ISSN (online)	2090-6692
ISSN	2090-6684
DOI	10.1155/2022/1143478
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Article ; Online: 21-Fold Higher COVID-19 Mortality Rate in Patients with Severe Renal Dysfunction on Admission.

Ghorbani, Hamidreza / Golshan, Alireza / Aghaee, Atena / Mottaghi, Mahdi / Soltani, Salman

Urology journal

2021

Language	English
Publishing date	2021-08-03
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2251940-3
ISSN	1735-546X ; 1735-1308
ISSN (online)	1735-546X
ISSN	1735-1308
DOI	10.22037/uj.v18i.6891
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Article ; Online: Prostate-Specific Membrane Antigen Radioguided Surgery for Salvage Pelvic Lymph Node Dissection in a Man With Prostate Cancer.

Erfani, Soheila / Sadeghi, Ramin / Aghaee, Atena / Ghorbani, HamidReza / Roshanravan, Vahid

Clinical nuclear medicine

2023 Volume 47, Issue 2, Page(s) e174–e176

Abstract: Abstract: A 75-year-old man with evidence of recurrent prostate cancer on 68Ga-prostate-specific membrane antigen (PSMA) PET/CT after radical prostatectomy was referred to our department for 99mTc-PSMA radioguided surgery. Intraoperatively, lymph nodes ... ...

Abstract	Abstract: A 75-year-old man with evidence of recurrent prostate cancer on 68Ga-prostate-specific membrane antigen (PSMA) PET/CT after radical prostatectomy was referred to our department for 99mTc-PSMA radioguided surgery. Intraoperatively, lymph nodes with high count rates were localized using a handheld gamma probe. High-count specimens were resected from the exact locations as reported by 68Ga-PSMA PET/CT. The total prostate-specific antigen value decreased 6 weeks after surgery. Our case shows the feasibility of radioguided surgery with 99mTc-PSMA for salvage lymphadenectomy in prostate cancer.
MeSH term(s)	Aged ; Gallium Isotopes ; Gallium Radioisotopes ; Humans ; Lymph Node Excision ; Lymphatic Metastasis ; Male ; Neoplasm Recurrence, Local ; Positron Emission Tomography Computed Tomography ; Prostate ; Prostatectomy ; Prostatic Neoplasms/surgery ; Radiopharmaceuticals ; Surgery, Computer-Assisted
Chemical Substances	Gallium Isotopes ; Gallium Radioisotopes ; Radiopharmaceuticals ; gallium 68 PSMA-11
Language	English
Publishing date	2023-09-18
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	197628-x
ISSN	1536-0229 ; 0363-9762
ISSN (online)	1536-0229
ISSN	0363-9762
DOI	10.1097/RLU.0000000000003944
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Zs.A 1276: Show issues

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Article ; Online: A Non-Apoptotic Pattern of Caspase-9/Caspase-3 Activation During Differentiation of Human Embryonic Stem Cells into Cardiomyocytes.

Ghorbani, Negar / Shiri, Mahshad / Alian, Maedeh / Yaghubi, Roham / Shafaghi, Mojtaba / Hojjat, Hamidreza / Pahlavan, Sara / Davoodi, Jamshid

Advanced biology

2024 , Page(s) e2400026

Abstract: In vitro studies have demonstrated that the differentiation of embryonic stem cells (ESCs) into cardiomyocytes requires activation of caspases through the mitochondrial pathway. These studies have relied on synthetic substrates for activity measurements, ...

Abstract	In vitro studies have demonstrated that the differentiation of embryonic stem cells (ESCs) into cardiomyocytes requires activation of caspases through the mitochondrial pathway. These studies have relied on synthetic substrates for activity measurements, which can be misleading due to potential none-specific hydrolysis of these substrates by proteases other than caspases. Hence, caspase-9 and caspase-3 activation are investigated during the differentiation of human ESCs (hESCs) by directly assessing caspase-9 and -3 cleavage. Western blot reveals the presence of the cleaved caspase-9 prior to and during the differentiation of human ESCs (hESCs) into cardiomyocytes at early stages, which diminishes as the differentiation progresses, without cleavage and activation of endogenous procaspase-3. Activation of exogenous procaspase-3 by endogenous caspase-9 and subsequent cleavage of chromogenic caspase-3 substrate i.e. DEVD-pNA during the course of differentiation confirmes that endogenous caspase-9 has the potency to recognize and activate procaspase-3, but for reasons that are unknown to us fails to do so. These observations suggest the existence of distinct mechanisms of caspase regulation in differentiation as compared to apoptosis. Bioinformatics analysis suggests the presence of caspase-9 regulators, which may influence proteolytic function under specific conditions.
Language	English
Publishing date	2024-04-19
Publishing country	Germany
Document type	Journal Article
ISSN	2701-0198
ISSN (online)	2701-0198
DOI	10.1002/adbi.202400026
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Article: Adult Wilms with Biphasic Pattern; A Case Report.

Farzadnia, Mahdi / Karrabi, Mahboobe / Ghorbani, Hamidreza

Caspian journal of internal medicine

2021 Volume 12, Issue Suppl 2, Page(s) S421–S425

Abstract: Background: Wilms' tumor, nephroblastoma, is an extremely uncommon kidney tumor of adulthood. We reported a woman with a huge kidney mass diagnosed with nephroblastoma.: Case presentation: A 39-year-old female was assessed due to right flank pain. CT ...

Abstract	Background: Wilms' tumor, nephroblastoma, is an extremely uncommon kidney tumor of adulthood. We reported a woman with a huge kidney mass diagnosed with nephroblastoma. Case presentation: A 39-year-old female was assessed due to right flank pain. CT scan revealed a mass measuring 128×100 mms involving the upper portion of the right kidney. The patient underwent nephrectomy, and the diagnosis of adult Wilms' tumor was confirmed based on the morphological and immunohistochemical findings. Conclusion: In adult patients with flank pain and renal mass, the diagnosis of Wilms' tumor should be pronounced in the absence of histopathologic features of renal cell carcinoma.
Language	English
Publishing date	2021-10-21
Publishing country	Iran
Document type	Case Reports
ZDB-ID	2971933-1
ISSN	2008-6172 ; 2008-6164
ISSN (online)	2008-6172
ISSN	2008-6164
DOI	10.22088/cjim.12.0.421
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Article ; Online: SARS-CoV-2 nucleocapsid: Biological functions and implication for disease diagnosis and vaccine design.

Maghsood, Faezeh / Ghorbani, Ahmad / Yadegari, Hamidreza / Golsaz-Shirazi, Forough / Amiri, Mohammad Mehdi / Shokri, Fazel

Reviews in medical virology

2023 Volume 33, Issue 3, Page(s) e2431

Abstract: The coronavirus disease 2019 (COVID-19) pandemic is transmitted by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has affected millions of people all around the world, leading to more than 6.5 million deaths. The nucleocapsid (N) ... ...

Abstract	The coronavirus disease 2019 (COVID-19) pandemic is transmitted by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has affected millions of people all around the world, leading to more than 6.5 million deaths. The nucleocapsid (N) phosphoprotein plays important roles in modulating viral replication and transcription, virus-infected cell cycle progression, apoptosis, and regulation of host innate immunity. As an immunodominant protein, N protein induces strong humoral and cellular immune responses in COVID-19 patients, making it a key marker for studying N-specific B cell and T cell responses and the development of diagnostic serological assays and efficient vaccines. In this review, we focus on the structural and functional features and the kinetic and epitope mapping of B cell and T cell responses against SARS-CoV-2 N protein to extend our understanding on the development of sensitive and specific diagnostic immunological tests and effective vaccines.
MeSH term(s)	Humans ; SARS-CoV-2 ; COVID-19/diagnosis ; Nucleocapsid Proteins/chemistry ; Nucleocapsid Proteins/metabolism ; COVID-19 Vaccines ; Nucleocapsid/metabolism ; COVID-19 Testing
Chemical Substances	Nucleocapsid Proteins ; COVID-19 Vaccines
Language	English
Publishing date	2023-02-15
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	1086043-5
ISSN	1099-1654 ; 1052-9276
ISSN (online)	1099-1654
ISSN	1052-9276
DOI	10.1002/rmv.2431
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Article ; Online: Renal vein thrombosis due to metastatic germ cell tumor, report of a case with a very rare clinical scenario.

Ghorbani, Hamidreza / Emadi Torghabeh, Ali / Farzadnia, Mahdi / Golshan, Alireza / Rabiei, Parisa

Cancer reports (Hoboken, N.J.)

2023 Volume 6, Issue 12, Page(s) e1910

Abstract: Background: Renal metastasis is a rare manifestation of germ cell tumors. Extension of malignant lesions into the renal vein can complicate the scenario.: Case: This report presents a 35-year-old man with primary stage IS NSGCT. Fourteen months after ...

Abstract	Background: Renal metastasis is a rare manifestation of germ cell tumors. Extension of malignant lesions into the renal vein can complicate the scenario. Case: This report presents a 35-year-old man with primary stage IS NSGCT. Fourteen months after radical orchiectomy he presented with metastasis in the lung, kidney, and para-aortic lymph nodes. He received multiple lines of salvage treatments including chemotherapy and surgery. Intraoperative exploration during radical nephrectomy and retroperitoneal lymphadenectomy revealed intra-renal vein involvement with a prominent teratomatous component. Conclusion: Defining the exact extent of malignant lesions, especially endovascular lesions, is very important to clarify how advanced the malignant lesions are. The surgeons must be aware of the risk factors that predict vascular involvement, and therefore, providing intraoperative access to vascular surgery procedures when needed.
MeSH term(s)	Male ; Humans ; Adult ; Testicular Neoplasms/diagnosis ; Testicular Neoplasms/surgery ; Renal Veins/surgery ; Renal Veins/pathology ; Neoplasms, Germ Cell and Embryonal/diagnosis ; Neoplasms, Germ Cell and Embryonal/surgery ; Lymph Node Excision/methods ; Neoplasms, Second Primary ; Thrombosis/surgery
Language	English
Publishing date	2023-10-08
Publishing country	United States
Document type	Case Reports
ISSN	2573-8348
ISSN (online)	2573-8348
DOI	10.1002/cnr2.1910
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Article ; Online: Economical Evaluation of Prostate Cancer Treatment Using Intensity-Modulated Radiation Therapy, 3-Dimensional Conformal Radiation Therapy and Radical Prostatectomy: A Systematic Review.

Adel, Amin / Rezapour, Aziz / Aboutorabi, Ali / Taghizadeh Kermani, Ali / Ghorbani, Hamidreza

Value in health regional issues

2023 Volume 39, Page(s) 57–65

Abstract: Objectives: Prostate cancer is a common form of cancer among men worldwide. The objective of this study was to conduct a systematic review of the economic evaluations of prostate cancer treatment strategies.: Methods: This systematic review was ... ...

Abstract	Objectives: Prostate cancer is a common form of cancer among men worldwide. The objective of this study was to conduct a systematic review of the economic evaluations of prostate cancer treatment strategies. Methods: This systematic review was conducted using multiple electronic databases up to May 2021. English-language economic evaluation studies that compared intensity-modulated radiation therapy (IMRT), 3-dimensional conformal radiation therapy (3DCRT), and radical prostatectomy (RP) were included. The studies were evaluated using the Consolidated Health Economic Evaluation Reporting Standards checklist. The search yielded 1151 potentially relevant publications, which were screened based on the title and abstract. After the removal of duplicates, 55 studies remained, and 9 studies were screened in full text. Finally, textual data were analyzed manually using by-content analysis method. Results: All studies were cost-effective and evaluated quality-adjusted life year as the efficacy indicator. The studies were conducted from either payers' or health systems' perspectives, and the time horizon varied from 5 to 20 years. We included only full economic evaluation studies. The use of IMRT in comparison with 3DCRT was evaluated in 6 studies, based on which IMRT increased health and reduced side effects of treatment. According to incremental cost-effectiveness ratio (ICER) results, IMRT was more cost-effective than 3DCRT. Three studies evaluated the use of RP in comparison with radiotherapy. Based on these studies, radiotherapy was more effective than RP. Conclusion: IMRT was found to be more cost-effective than 3DCRT in all 6 studies compared with the threshold. Radiotherapy was found to be more effective than RP. However, long-term clinical trial studies are needed to confirm these findings and to provide more definitive conclusions.
MeSH term(s)	Male ; Humans ; Radiotherapy, Intensity-Modulated/adverse effects ; Radiotherapy, Intensity-Modulated/methods ; Radiotherapy, Conformal/adverse effects ; Radiotherapy, Conformal/methods ; Prostatic Neoplasms/radiotherapy ; Prostatic Neoplasms/surgery ; Prostatectomy ; Cost-Benefit Analysis
Language	English
Publishing date	2023-11-17
Publishing country	United States
Document type	Systematic Review ; Journal Article
ZDB-ID	2679127-4
ISSN	2212-1102 ; 2212-1099
ISSN (online)	2212-1102
ISSN	2212-1099
DOI	10.1016/j.vhri.2023.08.009
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