Article: Improvement of putrescine production through the arginine decarboxylase pathway in Escherichia coli K-12.
2021 Volume 11, Issue 1, Page(s) 168
Abstract: In the bio-based polymer industry, putrescine is in the spotlight for use as a material. We constructed strains of Escherichia coli to assess its putrescine production capabilities through the arginine decarboxylase pathway in batch fermentation. N- ... ...
Abstract | In the bio-based polymer industry, putrescine is in the spotlight for use as a material. We constructed strains of Escherichia coli to assess its putrescine production capabilities through the arginine decarboxylase pathway in batch fermentation. N-Acetylglutamate (ArgA) synthase is subjected to feedback inhibition by arginine. Therefore, the 19th amino acid residue, Tyr, of argA was substituted with Cys to desensitize the feedback inhibition of arginine, resulting in improved putrescine production. The inefficient initiation codon GTG of argA was substituted with the effective ATG codon, but its replacement did not affect putrescine production. The essential genes for the putrescine production pathway, speA and speB, were cloned into the same plasmid with argA |
---|---|
Language | English |
Publishing date | 2021-12-15 |
Publishing country | Germany |
Document type | Journal Article |
ZDB-ID | 2621432-5 |
ISSN | 2191-0855 |
ISSN | 2191-0855 |
DOI | 10.1186/s13568-021-01330-5 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.