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Article: Serum Metabolomic Profiling to Reveal Potential Biomarkers for the Diagnosis of Fatty Liver Hemorrhagic Syndrome in Laying Hens.

Guo, Lianying / Kuang, Jun / Zhuang, Yu / Jiang, Jialin / Shi, Yan / Huang, Cheng / Zhou, Changming / Xu, Puzhi / Liu, Ping / Wu, Cong / Hu, Guoliang / Guo, Xiaoquan

Frontiers in physiology

2021 Volume 12, Page(s) 590638

Abstract: Fatty liver hemorrhage syndrome (FLHS), a nutritional and metabolic disease that frequently occurs in laying hens, causes serious losses to the poultry industry. Nowadays, the traditional clinical diagnosis of FLHS still has its limitations. Therefore, ... ...

Abstract	Fatty liver hemorrhage syndrome (FLHS), a nutritional and metabolic disease that frequently occurs in laying hens, causes serious losses to the poultry industry. Nowadays, the traditional clinical diagnosis of FLHS still has its limitations. Therefore, searching for some metabolic biomarkers and elucidating the metabolic pathway
Language	English
Publishing date	2021-02-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2021.590638
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Effects of subchronic exposure of mercuric chloride on intestinal histology and microbiota in the cecum of chicken.

Zhou, Changming / Xu, Puzhi / Huang, Cheng / Liu, Guohui / Chen, Shupeng / Hu, Guoliang / Li, Guyue / Liu, Ping / Guo, Xiaoquan

Ecotoxicology and environmental safety

2019 Volume 188, Page(s) 109920

Abstract: This study aimed to investigate the influences of mercuric chloride ( ... ...

Abstract	This study aimed to investigate the influences of mercuric chloride (HgCl
MeSH term(s)	Animals ; Bacteroidetes/isolation & purification ; Cecum/drug effects ; Cecum/microbiology ; Cecum/pathology ; Chickens/microbiology ; Dose-Response Relationship, Drug ; Environmental Pollutants/toxicity ; Gastrointestinal Microbiome/drug effects ; Gastrointestinal Microbiome/genetics ; Male ; Mercuric Chloride/toxicity ; Microbiota/genetics ; Proteobacteria/isolation & purification ; RNA, Ribosomal, 16S/genetics
Chemical Substances	Environmental Pollutants ; RNA, Ribosomal, 16S ; Mercuric Chloride (53GH7MZT1R)
Language	English
Publishing date	2019-11-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	436536-7
ISSN	1090-2414 ; 0147-6513
ISSN (online)	1090-2414
ISSN	0147-6513
DOI	10.1016/j.ecoenv.2019.109920
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Article: Corrigendum: Alterations of the Gut Microbiota in Multiple System Atrophy Patients.

Wan, Linlin / Zhou, Xin / Wang, Chunrong / Chen, Zhao / Peng, Huirong / Hou, Xuan / Peng, Yun / Wang, Puzhi / Li, Tianjiao / Yuan, Hongyu / Shi, Yuting / Hou, Xiaocan / Xu, Keqin / Xie, Yue / He, Lang / Xia, Kun / Tang, Beisha / Jiang, Hong

Frontiers in neuroscience

2020 Volume 14, Page(s) 19

Abstract: This corrects the article DOI: 10.3389/fnins.2019.01102.]. ...

Abstract	[This corrects the article DOI: 10.3389/fnins.2019.01102.].
Language	English
Publishing date	2020-01-31
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2411902-7
ISSN	1662-453X ; 1662-4548
ISSN (online)	1662-453X
ISSN	1662-4548
DOI	10.3389/fnins.2020.00019
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Article ; Online: Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken

Jun Kuang / Puzhi Xu / Yan Shi / Yitian Yang / Ping Liu / Shupeng Chen / Changming Zhou / Guyue Li / Yu Zhuang / Ruiming Hu / Guoliang Hu / Xiaoquan Guo

Frontiers in Veterinary Science, Vol

2021 Volume 7

Abstract: Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus ( ... ...

Abstract	Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus (NIBV) strains have increasingly emerged in recent years; hence, evaluating their infection-influenced immune function changes and the alteration of metabolite profiling is important. Initially, chickens were randomly distributed into two groups: the control group (Con) and the disease group (Dis). Here, the partial cytokines were examined, and the metabolome alterations of the bursa of Fabricius (BF) in NIBV infections in chickens were profiled by gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS). The results revealed that the NIBV infection promotes the mRNA expression of inflammatory cytokines. Metabolic profile analysis indicated that clustering differed between the two groups and there were 75 significantly different metabolites detected between the two groups, suggesting that the host metabolism was significantly changed by NIBV infection. Notably, the following 12 metabolites were identified as the potential biomarkers: 3-phenyllactic acid, 2-deoxytetronic acid, aminomalonic acid, malonamide 5, uric acid, arachidonic acid, 2-methylglutaric acid, linoleic acid, ethanolamine, stearic acid, N-alpha-acetyl-l-ornithine, and O-acetylserine. Furthermore, the results of the correlation analysis showed that a strong correlation existed between metabolic biomarkers and inflammatory cytokines. Our results describe an immune and metabolic profile for the BF of chickens when infected with NIBV and provide new biomarkers of NIBV infection as potential targets and indicators of indicating therapeutic efficacy.
Keywords	metabolome ; inflammatory cytokines ; bursa of fabricius ; nephropathogenic infectious bronchitis virus ; correlation analysis ; Veterinary medicine ; SF600-1100
Subject code	570
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken.

Kuang, Jun / Xu, Puzhi / Shi, Yan / Yang, Yitian / Liu, Ping / Chen, Shupeng / Zhou, Changming / Li, Guyue / Zhuang, Yu / Hu, Ruiming / Hu, Guoliang / Guo, Xiaoquan

Frontiers in veterinary science

2021 Volume 7, Page(s) 628270

Abstract	Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus (NIBV) strains have increasingly emerged in recent years; hence, evaluating their infection-influenced immune function changes and the alteration of metabolite profiling is important. Initially, chickens were randomly distributed into two groups: the control group (Con) and the disease group (Dis). Here, the partial cytokines were examined, and the metabolome alterations of the bursa of Fabricius (BF) in NIBV infections in chickens were profiled by gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS). The results revealed that the NIBV infection promotes the mRNA expression of inflammatory cytokines. Metabolic profile analysis indicated that clustering differed between the two groups and there were 75 significantly different metabolites detected between the two groups, suggesting that the host metabolism was significantly changed by NIBV infection. Notably, the following 12 metabolites were identified as the potential biomarkers: 3-phenyllactic acid, 2-deoxytetronic acid, aminomalonic acid, malonamide 5, uric acid, arachidonic acid, 2-methylglutaric acid, linoleic acid, ethanolamine, stearic acid, N-alpha-acetyl-l-ornithine, and O-acetylserine. Furthermore, the results of the correlation analysis showed that a strong correlation existed between metabolic biomarkers and inflammatory cytokines. Our results describe an immune and metabolic profile for the BF of chickens when infected with NIBV and provide new biomarkers of NIBV infection as potential targets and indicators of indicating therapeutic efficacy.
Language	English
Publishing date	2021-01-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2834243-4
ISSN	2297-1769
ISSN	2297-1769
DOI	10.3389/fvets.2020.628270
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Article: A Multi-Omics Study of Chicken Infected by Nephropathogenic Infectious Bronchitis Virus

Xu, Puzhi / Liu, Ping / Zhou, Changming / Shi, Yan / Wu, Qingpeng / Yang, Yitian / Li, Guyue / Hu, Guoliang / Guo, Xiaoquan

Viruses. 2019 Nov. 16, v. 11, no. 11

2019

Abstract: Chicken gout resulting from nephropathogenic infectious bronchitis virus (NIBV) has become a serious kidney disease problem in chicken worldwide with alterations of the metabolic phenotypes in multiple metabolic pathways. To investigate the mechanisms in ...

Abstract	Chicken gout resulting from nephropathogenic infectious bronchitis virus (NIBV) has become a serious kidney disease problem in chicken worldwide with alterations of the metabolic phenotypes in multiple metabolic pathways. To investigate the mechanisms in chicken responding to NIBV infection, we examined the global transcriptomic and metabolomic profiles of the chicken’s kidney using RNA-seq and GC–TOF/MS, respectively. Furthermore, we analyzed the alterations in cecal microorganism composition in chickens using 16S rRNA-seq. Integrated analysis of these three phenotypic datasets further managed to create correlations between the altered kidney transcriptomes and metabolome, and between kidney metabolome and gut microbiome. We found that 2868 genes and 160 metabolites were deferentially expressed or accumulated in the kidney during NIBV infection processes. These genes and metabolites were linked to NIBV-infection related processes, including immune response, signal transduction, peroxisome, purine, and amino acid metabolism. In addition, the comprehensive correlations between the kidney metabolome and cecal microbial community showed contributions of gut microbiota in the progression of NIBV-infection. Taken together, our research comprehensively describes the host responses during NIBV infection and provides new clues for further dissection of specific gene functions, metabolite affections, and the role of gut microbiota during chicken gout.
Keywords	Infectious bronchitis virus ; amino acid metabolism ; biochemical pathways ; chickens ; data collection ; genes ; gout ; immune response ; intestinal microorganisms ; kidney diseases ; kidneys ; metabolites ; metabolome ; metabolomics ; microbial communities ; multiomics ; phenotype ; sequence analysis ; signal transduction ; transcriptome ; transcriptomics ; covid19
Language	English
Dates of publication	2019-1116
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v11111070
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A Multi-Omics Study of Chicken Infected by Nephropathogenic Infectious Bronchitis Virus.

Xu, Puzhi / Liu, Ping / Zhou, Changming / Shi, Yan / Wu, Qingpeng / Yang, Yitian / Li, Guyue / Hu, Guoliang / Guo, Xiaoquan

Viruses

2019 Volume 11, Issue 11

Abstract	Chicken gout resulting from nephropathogenic infectious bronchitis virus (NIBV) has become a serious kidney disease problem in chicken worldwide with alterations of the metabolic phenotypes in multiple metabolic pathways. To investigate the mechanisms in chicken responding to NIBV infection, we examined the global transcriptomic and metabolomic profiles of the chicken's kidney using RNA-seq and GC-TOF/MS, respectively. Furthermore, we analyzed the alterations in cecal microorganism composition in chickens using 16S rRNA-seq. Integrated analysis of these three phenotypic datasets further managed to create correlations between the altered kidney transcriptomes and metabolome, and between kidney metabolome and gut microbiome. We found that 2868 genes and 160 metabolites were deferentially expressed or accumulated in the kidney during NIBV infection processes. These genes and metabolites were linked to NIBV-infection related processes, including immune response, signal transduction, peroxisome, purine, and amino acid metabolism. In addition, the comprehensive correlations between the kidney metabolome and cecal microbial community showed contributions of gut microbiota in the progression of NIBV-infection. Taken together, our research comprehensively describes the host responses during NIBV infection and provides new clues for further dissection of specific gene functions, metabolite affections, and the role of gut microbiota during chicken gout.
MeSH term(s)	Animals ; Biopsy ; Chickens/virology ; Computational Biology/methods ; Coronavirus Infections/veterinary ; Dysbiosis ; Gas Chromatography-Mass Spectrometry ; Gastrointestinal Microbiome ; Gene Expression Profiling ; Genomics/methods ; Host-Pathogen Interactions ; Immunohistochemistry ; Infectious bronchitis virus/physiology ; Metabolomics/methods ; Organ Specificity ; Poultry Diseases/diagnosis ; Poultry Diseases/genetics ; Poultry Diseases/metabolism ; Poultry Diseases/virology ; Symptom Assessment ; Transcriptome
Keywords	covid19
Language	English
Publishing date	2019-11-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v11111070
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: 16S rRNA gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus

Puzhi Xu / Yan Shi / Ping Liu / Yitian Yang / Changming Zhou / Guyue Li / Junrong Luo / Caiying Zhang / Huabin Cao / Guoliang Hu / Xiaoquan Guo

Scientific Reports, Vol 10, Iss 1, Pp 1-

2020 Volume 12

Abstract: Abstract Infectious bronchitis virus (IBV), a member of the Coronaviridae family, causes serious losses to the poultry industry. Intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading ...

Abstract	Abstract Infectious bronchitis virus (IBV), a member of the Coronaviridae family, causes serious losses to the poultry industry. Intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading pathogens. The aim of this study was to investigate the link between the intestinal microbiome and nephropathogenic IBV (NIBV) infection. Initially, chickens were randomly distributed into 2 groups: the normal group (INC) and the infected group (IIBV). The ilea were collected for morphological assessment, and the ileal contents were collected for 16S rRNA gene sequencing analysis. The results of the IIBV group analyses showed a significant decrease in the ratio of villus height to crypt depth (P < 0.05), while the goblet cells increased compared to those in the INC group. Furthermore, the microbial diversity in the ilea decreased and overrepresentation of Enterobacteriaceae and underrepresentation of Chloroplast and Clostridia was found in the NIBV-infected chickens. In conclusion, these results showed that the significant separation of the two groups and the characterization of the gut microbiome profiles of the chickens with NIBV infection may provide valuable information and promising biomarkers for the diagnosis of this disease.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2020-02-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: 16S rRNA gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus.

Xu, Puzhi / Shi, Yan / Liu, Ping / Yang, Yitian / Zhou, Changming / Li, Guyue / Luo, Junrong / Zhang, Caiying / Cao, Huabin / Hu, Guoliang / Guo, Xiaoquan

Scientific reports

2020 Volume 10, Issue 1, Page(s) 3556

Abstract: Infectious bronchitis virus (IBV), a member of the Coronaviridae family, causes serious losses to the poultry industry. Intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading ... ...

Abstract	Infectious bronchitis virus (IBV), a member of the Coronaviridae family, causes serious losses to the poultry industry. Intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading pathogens. The aim of this study was to investigate the link between the intestinal microbiome and nephropathogenic IBV (NIBV) infection. Initially, chickens were randomly distributed into 2 groups: the normal group (INC) and the infected group (IIBV). The ilea were collected for morphological assessment, and the ileal contents were collected for 16S rRNA gene sequencing analysis. The results of the IIBV group analyses showed a significant decrease in the ratio of villus height to crypt depth (P < 0.05), while the goblet cells increased compared to those in the INC group. Furthermore, the microbial diversity in the ilea decreased and overrepresentation of Enterobacteriaceae and underrepresentation of Chloroplast and Clostridia was found in the NIBV-infected chickens. In conclusion, these results showed that the significant separation of the two groups and the characterization of the gut microbiome profiles of the chickens with NIBV infection may provide valuable information and promising biomarkers for the diagnosis of this disease.
MeSH term(s)	Animals ; Biodiversity ; Chickens ; Computational Biology/methods ; Coronavirus Infections/veterinary ; Gastrointestinal Microbiome ; High-Throughput Nucleotide Sequencing ; Infectious bronchitis virus/physiology ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/microbiology ; Intestinal Mucosa/pathology ; Metagenome ; Metagenomics/methods ; Poultry Diseases/etiology ; RNA, Ribosomal, 16S ; Viral Load
Chemical Substances	RNA, Ribosomal, 16S
Keywords	covid19
Language	English
Publishing date	2020-02-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-60564-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Cloning and prokaryotic expression of the chicken liver kinase B1 (LKB1) and its localization in liver, heart and hypothalamus.

Zhang, Kun / Shi, Yan / Xu, Puzhi / Huang, Cheng / Zhou, Changming / Liu, Ping / Hu, Ruiming / Zhuang, Yu / Li, Guyue / Hu, Guoliang / Guo, Xiaoquan

International journal of biological macromolecules

2020 Volume 169, Page(s) 513–520

Abstract: Liver kinase B1 (LKB1) is a member of the serine/threonine kinase family, which plays an indispensable role in the organism of animals. In the current study, the chicken LKB1 protein gene was amplified by PCR based on the primers and cDNA templates. Then, ...

Abstract	Liver kinase B1 (LKB1) is a member of the serine/threonine kinase family, which plays an indispensable role in the organism of animals. In the current study, the chicken LKB1 protein gene was amplified by PCR based on the primers and cDNA templates. Then, the cloning vector was constructed and the target gene was cloned. After that, the target gene was inserted into the expression vector to construct the recombinant plasmid. The recombinant plasmid was transformed into BL21 (DE3) host cells and the LKB1 recombinant proteins were successfully expressed by using Isopropyl-β-D-thiogalactopyranoside (IPTG). Finally, purified LKB1 proteins were used as antigen and the rabbit-derived antiserums were collected. The antiserum titer determined by ELISA was not less than 1:128000. The results of Western blot suggested that the polyclonal antibody is highly specific to chicken LKB1 protein. Immunofluorescence indicated that the LKB1 protein is mainly expressed in the cytoplasm of liver, heart and hypothalamus cells of chicken. Our study showed that the LKB1 polyclonal antibodies produced by this method are effective and can be used to further study the role of LKB1 in the pathogenesis of chicken disease.
MeSH term(s)	Animals ; Antibodies/immunology ; Antibody Specificity/immunology ; Chickens/genetics ; Chickens/metabolism ; Cloning, Molecular/methods ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli/genetics ; Gene Expression/genetics ; Genetic Vectors/genetics ; Hypothalamus/metabolism ; Immune Sera/immunology ; Liver/metabolism ; Myocardium/metabolism ; Polymerase Chain Reaction ; Protein-Serine-Threonine Kinases/genetics ; Protein-Serine-Threonine Kinases/metabolism ; Recombinant Proteins/genetics
Chemical Substances	Antibodies ; Immune Sera ; Recombinant Proteins ; Protein-Serine-Threonine Kinases (EC 2.7.11.1)
Language	English
Publishing date	2020-12-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2020.12.195
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