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  1. Article: Diversity of dendritic cells generated from umbilical cord or adult peripheral blood precursors.

    Szaryńska, Magdalena / Preis, Krzysztof / Zabul, Piotr / Kmieć, Zbigniew

    Central-European journal of immunology

    2018  Volume 43, Issue 3, Page(s) 306–313

    Abstract: Following the discovery of methods to generate large numbers of specific dendritic cells (DCs) ex vivo, the possibility of exploiting these cells in immunotherapeutic strategies will become a reality. It seems to be rationally to analyse the influence of ...

    Abstract Following the discovery of methods to generate large numbers of specific dendritic cells (DCs) ex vivo, the possibility of exploiting these cells in immunotherapeutic strategies will become a reality. It seems to be rationally to analyse the influence of the precursor source for further features and applications. For the needs of the given project DCs were derived from precursors derived from adult peripheral blood (APB) and umbilical cord blood (UCB). During some expansions of UCB CD34
    Language English
    Publishing date 2018-10-30
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 1336421-2
    ISSN 1644-4124 ; 1426-3912
    ISSN (online) 1644-4124
    ISSN 1426-3912
    DOI 10.5114/ceji.2018.80050
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Diversity of dendritic cells generated from umbilical cord or adult peripheral blood precursors

    Magdalena Szaryńska / Krzysztof Preis / Piotr Zabul / Zbigniew Kmieć

    Central European Journal of Immunology, Vol 43, Iss 3, Pp 306-

    2018  Volume 313

    Abstract: Following the discovery of methods to generate large numbers of specific dendritic cells (DCs) ex vivo, the possibility of exploiting these cells in immunotherapeutic strategies will become a reality. It seems to be rationally to analyse the influence of ...

    Abstract Following the discovery of methods to generate large numbers of specific dendritic cells (DCs) ex vivo, the possibility of exploiting these cells in immunotherapeutic strategies will become a reality. It seems to be rationally to analyse the influence of the precursor source for further features and applications. For the needs of the given project DCs were derived from precursors derived from adult peripheral blood (APB) and umbilical cord blood (UCB). During some expansions of UCB CD34+ cells were separated giving non-adherent DCs (NA-DCs) or adherent DCs (A-DCs), whereas DCs derived from UCB precursors without separation gave rise to All-DCs. DC subpopulations were stimulated by lipopolysaccharides (LPS) or interferon- (IFN-), and afterwards the morphology, phenotype, and stimulatory properties were analysed. Our findings demonstrated that DCs generated from APB and UCB precursors were not equivalent and exhibited opposite features when expanded in comparable conditions. Additionally, all three subpopulations of UCB-derived DCs presented functional dissimilarities. Based on our results we concluded that the precursor source and the composition of media must be considered as crucial to the success of potential therapeutic application.
    Keywords dendritic cells ; haematopoietic stem cells ; monocytes ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2018-10-01T00:00:00Z
    Publisher Termedia Publishing House
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Cytokine profiles during delivery affect cord blood hematopoietic stem and progenitors cells.

    Szaryńska, Magdalena / Myśliwski, Andrzej / Myśliwska, Jolanta / Kmieć, Zbigniew / Preis, Krzysztof / Zabul, Piotr

    Cellular immunology

    2015  Volume 293, Issue 2, Page(s) 137–141

    Abstract: The study was aimed to determine the correlations between serum levels of cytokines (GM-CSF, IL-4, IL-10 and TNF) in maternal (MB) and cord blood (CB) and some features of cord blood hematopoietic stem and progenitor cells (CB HSPCs). Study material was ... ...

    Abstract The study was aimed to determine the correlations between serum levels of cytokines (GM-CSF, IL-4, IL-10 and TNF) in maternal (MB) and cord blood (CB) and some features of cord blood hematopoietic stem and progenitor cells (CB HSPCs). Study material was MB and concomitant CB samples collected from 98 volunteers at the moment of delivery. The IL-4, IL-10, TNF and GM-CSF concentrations in serum and in supernatants from PMA-stimulated mononuclear cells isolated from both blood types were measured using BD Cytometric Bead Array Flex Set System. CB HSPCs (CD34(+)CD45(low)) proportion was also estimated by flow cytometry. The most relevant results concerned the tendency to down regulation of CB HSPCs number with an increase of IL-4, IL-10 and GM-CSF levels, only the TNF concentration seems to have no influence on HSPCs pole size. The strongest positive correlations were found between CD34(+)CD45(low) HSPCs number and IL-10 and GM-CSF in MB serum and GM-CSF and TNF from CB supernatants. The strongest negative correlations were found between CD34(+)CD45(low) HSPCs number and IL-4 and GM-CSF in CB serum and IL-10 in MB supernatants. Interestingly, we observed 'opposite correlation' between serum and supernatant from CB and MB. We concluded that elevated serum levels of IL-4, IL-10 and GM-CSF in CB are indicative of enhanced differentiation of HSPCs and characterize a normal perinatal development. Elevated levels of cytokines seem to stimulate differentiation of HSPCs what is advantageous for neonates during perinatal period.
    MeSH term(s) Adolescent ; Adult ; Female ; Fetal Blood/cytology ; Fetal Blood/immunology ; Flow Cytometry ; Granulocyte-Macrophage Colony-Stimulating Factor/blood ; Hematopoietic Stem Cells/immunology ; Humans ; Infant, Newborn ; Interleukin-10/blood ; Interleukin-4/blood ; Stem Cells/immunology ; Tumor Necrosis Factor-alpha/blood
    Chemical Substances IL10 protein, human ; IL4 protein, human ; Tumor Necrosis Factor-alpha ; Interleukin-10 (130068-27-8) ; Interleukin-4 (207137-56-2) ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1)
    Language English
    Publishing date 2015-02
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80094-6
    ISSN 1090-2163 ; 0008-8749
    ISSN (online) 1090-2163
    ISSN 0008-8749
    DOI 10.1016/j.cellimm.2015.01.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 417: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: A Proposed Molecular Mechanism of High-Dose Vitamin D3 Supplementation in Prevention and Treatment of Preeclampsia.

    Zabul, Piotr / Wozniak, Michal / Slominski, Andrzej T / Preis, Krzysztof / Gorska, Magdalena / Korozan, Marek / Wieruszewski, Jan / Zmijewski, Michal A / Zabul, Ewa / Tuckey, Robert / Kuban-Jankowska, Alicja / Mickiewicz, Wieslawa / Knap, Narcyz

    International journal of molecular sciences

    2015  Volume 16, Issue 6, Page(s) 13043–13064

    Abstract: A randomized prospective clinical study performed on a group of 74 pregnant women (43 presenting with severe preeclampsia) proved that urinary levels of 15-F(2t)-isoprostane were significantly higher in preeclamptic patients relative to the control (3.05 ...

    Abstract A randomized prospective clinical study performed on a group of 74 pregnant women (43 presenting with severe preeclampsia) proved that urinary levels of 15-F(2t)-isoprostane were significantly higher in preeclamptic patients relative to the control (3.05 vs. 2.00 ng/mg creatinine). Surprisingly enough, plasma levels of 25-hydroxyvitamin D3 in both study groups were below the clinical reference range with no significant difference between the groups. In vitro study performed on isolated placental mitochondria and placental cell line showed that suicidal self-oxidation of cytochrome P450scc may lead to structural disintegration of heme, potentially contributing to enhancement of oxidative stress phenomena in the course of preeclampsia. As placental cytochrome P450scc pleiotropic activity is implicated in the metabolism of free radical mediated arachidonic acid derivatives as well as multiple Vitamin D3 hydroxylations and progesterone synthesis, we propose that Vitamin D3 might act as a competitive inhibitor of placental cytochrome P450scc preventing the production of lipid peroxides or excess progesterone synthesis, both of which may contribute to the etiopathogenesis of preeclampsia. The proposed molecular mechanism is in accord with the preliminary clinical observations on the surprisingly high efficacy of high-dose Vitamin D3 supplementation in prevention and treatment of preeclampsia.
    MeSH term(s) Adult ; Arachidonic Acid/metabolism ; Calcifediol/pharmacology ; Calcifediol/therapeutic use ; Cholesterol Side-Chain Cleavage Enzyme/metabolism ; Female ; Humans ; Lipid Peroxidation/drug effects ; Placenta/metabolism ; Pre-Eclampsia/drug therapy ; Pre-Eclampsia/prevention & control ; Pregnancy ; Vitamins/pharmacology ; Vitamins/therapeutic use
    Chemical Substances Vitamins ; Arachidonic Acid (27YG812J1I) ; Cholesterol Side-Chain Cleavage Enzyme (EC 1.14.15.6) ; Calcifediol (P6YZ13C99Q)
    Language English
    Publishing date 2015-06-09
    Publishing country Switzerland
    Document type Controlled Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms160613043
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A Proposed Molecular Mechanism of High-Dose Vitamin D3 Supplementation in Prevention and Treatment of Preeclampsia

    Piotr Zabul / Michal Wozniak / Andrzej T. Slominski / Krzysztof Preis / Magdalena Gorska / Marek Korozan / Jan Wieruszewski / Michal A. Zmijewski / Ewa Zabul / Robert Tuckey / Alicja Kuban-Jankowska / Wieslawa Mickiewicz / Narcyz Knap

    International Journal of Molecular Sciences, Vol 16, Iss 6, Pp 13043-

    2015  Volume 13064

    Abstract: A randomized prospective clinical study performed on a group of 74 pregnant women (43 presenting with severe preeclampsia) proved that urinary levels of 15-F2t-isoprostane were significantly higher in preeclamptic patients relative to the control (3.05 ... ...

    Abstract A randomized prospective clinical study performed on a group of 74 pregnant women (43 presenting with severe preeclampsia) proved that urinary levels of 15-F2t-isoprostane were significantly higher in preeclamptic patients relative to the control (3.05 vs. 2.00 ng/mg creatinine). Surprisingly enough, plasma levels of 25-hydroxyvitamin D3 in both study groups were below the clinical reference range with no significant difference between the groups. In vitro study performed on isolated placental mitochondria and placental cell line showed that suicidal self-oxidation of cytochrome P450scc may lead to structural disintegration of heme, potentially contributing to enhancement of oxidative stress phenomena in the course of preeclampsia. As placental cytochrome P450scc pleiotropic activity is implicated in the metabolism of free radical mediated arachidonic acid derivatives as well as multiple Vitamin D3 hydroxylations and progesterone synthesis, we propose that Vitamin D3 might act as a competitive inhibitor of placental cytochrome P450scc preventing the production of lipid peroxides or excess progesterone synthesis, both of which may contribute to the etiopathogenesis of preeclampsia. The proposed molecular mechanism is in accord with the preliminary clinical observations on the surprisingly high efficacy of high-dose Vitamin D3 supplementation in prevention and treatment of preeclampsia.
    Keywords preeclampsia ; isoprostanes ; arachidonic acid hydroperoxide ; vitamin D3 ; placenta ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 610
    Language English
    Publishing date 2015-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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