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  1. Article ; Online: Unfolding of protein using MoS

    Yuan, Runyi / Zhang, Zhen / Wu, Gensheng / Zhang, Yin / Sha, Jingjie / Chen, Yunfei / Si, Wei

    Nanotechnology

    2024  Volume 35, Issue 13

    Abstract: Protein sequencing is crucial for understanding the complex mechanisms driving biological functions. However, proteins are usually folded in their native state and the mechanism of fast protein conformation transitions still remains unclear, which make ... ...

    Abstract Protein sequencing is crucial for understanding the complex mechanisms driving biological functions. However, proteins are usually folded in their native state and the mechanism of fast protein conformation transitions still remains unclear, which make protein sequencing challenging. Molecular dynamics simulations with accurate force field are now able to observe the entire folding/unfolding process, providing valuable insights into protein folding mechanisms. Given that proteins can be unfolded, nanopore technology shows great potential for protein sequencing. In this study, we proposed to use MoS
    MeSH term(s) Molybdenum ; Nanopores ; Protein Folding ; Protein Unfolding ; Proteins/chemistry
    Chemical Substances Molybdenum (81AH48963U) ; Proteins ; tin sulfide (12738-87-3)
    Language English
    Publishing date 2024-01-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 1362365-5
    ISSN 1361-6528 ; 0957-4484
    ISSN (online) 1361-6528
    ISSN 0957-4484
    DOI 10.1088/1361-6528/ad177f
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Direct Evidence Demonstrates that

    Du, Zhimin / Peng, Yuelin / Zhang, Gensheng / Chen, Li / Jiang, Shuchang / Kang, Zhensheng / Zhao, Jie

    Plant disease

    2023  Volume 107, Issue 3, Page(s) 771–783

    Abstract: Wheat stripe rust is an airborne and destructive disease caused by a heteroecious rust ... ...

    Abstract Wheat stripe rust is an airborne and destructive disease caused by a heteroecious rust fungus
    MeSH term(s) Berberis/microbiology ; Seasons ; Genotype ; Genetic Linkage ; Basidiomycota
    Language English
    Publishing date 2023-03-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-08-22-1750-RE
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  3. Article: Effect of

    Zhang, Peng / Meng, Siyu / Bao, Gensheng / Li, Yuan / Feng, Xiaoyun / Lu, Hainian / Ma, Jingjuan / Wei, Xiaoxing / Liu, Wenhui

    Microorganisms

    2023  Volume 11, Issue 11

    Abstract: ... ...

    Abstract Epichloë
    Language English
    Publishing date 2023-11-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11112761
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  4. Article ; Online: Selective Capture and Manipulation of DNA through Double Charged Nanopores.

    Lin, Xiaojing / Chen, Haonan / Wu, Gensheng / Zhao, Jiajia / Zhang, Yin / Sha, Jingjie / Si, Wei

    The journal of physical chemistry letters

    2024  Volume 15, Issue 19, Page(s) 5120–5129

    Abstract: In the past few decades, nanometer-scale pores have been employed as powerful tools for sensing biological molecules. Owing to its unique structure and properties, solid-state nanopores provide interesting opportunities for the development of DNA ... ...

    Abstract In the past few decades, nanometer-scale pores have been employed as powerful tools for sensing biological molecules. Owing to its unique structure and properties, solid-state nanopores provide interesting opportunities for the development of DNA sequencing technology. Controlling DNA translocation in nanopores is an important means of improving the accuracy of sequencing. Here we present a proof of principle study of accelerating DNA captured across targeted graphene nanopores using surface charge density and find the intrinsic mechanism of the combination of electroosmotic flow induced by charges of nanopore and electrostatic attraction/repulsion between the nanopore and ssDNA. The theoretical study performed here provides a new means for controlling DNA transport dynamics and makes better and cheaper application of graphene in molecular sequencing.
    MeSH term(s) Nanopores ; Graphite/chemistry ; DNA/chemistry ; Static Electricity ; DNA, Single-Stranded/chemistry ; Electroosmosis ; Sequence Analysis, DNA/methods
    Language English
    Publishing date 2024-05-06
    Publishing country United States
    Document type Journal Article
    ISSN 1948-7185
    ISSN (online) 1948-7185
    DOI 10.1021/acs.jpclett.4c00672
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: An updated meta-analysis of modified early warning scores in patients with sepsis outside intensive care unit.

    Zhang, Kai / Zhang, Gensheng

    The Journal of infection

    2020  Volume 81, Issue 1, Page(s) e100–e101

    MeSH term(s) Early Warning Score ; Humans ; Intensive Care Units ; Sepsis/diagnosis
    Language English
    Publishing date 2020-04-22
    Publishing country England
    Document type Letter ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2020.04.015
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1501: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 48: Show issues

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  6. Article ; Online: Protein Deceleration and Sequencing Using Si

    Si, Wei / Zhang, Zhen / Chen, Jiayi / Wu, Gensheng / Zhang, Yin / Sha, Jingjie

    Chemphyschem : a European journal of chemical physics and physical chemistry

    2024  Volume 25, Issue 7, Page(s) e202300866

    Abstract: Protein sequencing is crucial for understanding the complex mechanisms driving biological functions and is of utmost importance in molecular diagnostics and medication development. Nanopores have become an effective tool for single molecule sensing, ... ...

    Abstract Protein sequencing is crucial for understanding the complex mechanisms driving biological functions and is of utmost importance in molecular diagnostics and medication development. Nanopores have become an effective tool for single molecule sensing, however, the weak charge and non-uniform charge distribution of protein make capturing and sensing very challenging, which poses a significant obstacle to the development of nanopore-based protein sequencing. In this study, to facilitate capturing of the unfolded protein, highly charged peptide was employed in our simulations, we found that the velocity of unfolded peptide translocating through a hybrid nanopore composed of silicon nitride membrane and carbon nanotube is much slower compared to bare silicon nitride nanopore, it is due to the significant interaction between amino acids and the surface of carbon nanotube. Moreover, by introducing variations in the charge states at the boundaries of carbon nanotube nanopores, the competition and combination of the electrophoretic and electroosmotic flows through the nanopores could be controlled, we then successfully regulated the translocation velocity of unfolded proteins through the hybrid nanopores. The proposed hybrid nanopore effectively retards the translocation velocity of protein through it, facilitates the acquisition of ample information for accurate amino acid identification.
    MeSH term(s) Nanopores ; Deceleration ; Nanotubes, Carbon ; Proteins ; Amino Acids ; Peptides ; Silicon Compounds
    Chemical Substances silicon nitride (QHB8T06IDK) ; Nanotubes, Carbon ; Proteins ; Amino Acids ; Peptides ; Silicon Compounds
    Language English
    Publishing date 2024-02-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2025223-7
    ISSN 1439-7641 ; 1439-4235
    ISSN (online) 1439-7641
    ISSN 1439-4235
    DOI 10.1002/cphc.202300866
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  7. Article ; Online: Probiotic

    Zhao, Zejing / Xu, Shumin / Zhang, Wangyang / Wu, Danjun / Yang, Gensheng

    Food & function

    2022  Volume 13, Issue 11, Page(s) 5914–5924

    Abstract: Escherichia ... ...

    Abstract Escherichia coli
    MeSH term(s) Colitis, Ulcerative/microbiology ; Diarrhea ; Escherichia coli ; Escherichia coli Infections ; Humans ; Probiotics/therapeutic use
    Language English
    Publishing date 2022-06-06
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2612033-1
    ISSN 2042-650X ; 2042-6496
    ISSN (online) 2042-650X
    ISSN 2042-6496
    DOI 10.1039/d2fo00226d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 7872: Show issues

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  8. Article ; Online: Electroosmotic Sensing of Uncharged Peptides and Differentiating Their Phosphorylated States Using Nanopores.

    Si, Wei / Chen, Jiayi / Zhang, Zhen / Wu, Gensheng / Zhao, Jiajia / Sha, Jingjie

    Chemphyschem : a European journal of chemical physics and physical chemistry

    2024  , Page(s) e202400281

    Abstract: The correct characterization and identification of different kinds of proteins is crucial for the survival and development of living organisms, and proteomics research promotes the analysis and understanding of future genome functions. Nanopore technique ...

    Abstract The correct characterization and identification of different kinds of proteins is crucial for the survival and development of living organisms, and proteomics research promotes the analysis and understanding of future genome functions. Nanopore technique has been proved to accurately identify individual nucleotides. However, accurate and rapid protein sequencing is difficult due to the variability of protein structures that contains more than 20 amino acids, and it remains very challenging especially for uncharged peptides as they can not be electrophoretically driven through the nanopore. Graphene nanopores have the advantages of high accuracy, sensitivity and low cost in identifying protein phosphorylation modifications. Here, by using all-atom molecular dynamics simulations, charged graphene nanopores are employed to electroosmotically capture and sense uncharged peptides. By further mimicking AFM manipulation of single molecules, it is also found that the uncharged peptides and their phosphorylated states could also be differentiated by both the ionic current and pulling force signals during their pulling processes through the nanopore with a slow and constant velocity. The results shows ability of using nanopores to detect and discriminate single amino acid and its phosphorylation, which is essential for the future low-cost and high-throughput sequencing of protein residues and their post-translational modifications.
    Language English
    Publishing date 2024-04-30
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2025223-7
    ISSN 1439-7641 ; 1439-4235
    ISSN (online) 1439-7641
    ISSN 1439-4235
    DOI 10.1002/cphc.202400281
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  9. Article ; Online: Intraventricular or intrathecal polymyxin B for treatment of post-neurosurgical intracranial infection caused by carbapenem-resistant gram-negative bacteria: a 8-year retrospective study.

    Hu, Yangmin / Li, Danyang / Zhang, Gensheng / Dai, Yunjian / Chen, Meng / Jiang, Huifang / Cui, Wei

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

    2024  

    Abstract: Purpose: Post-neurosurgical intracranial infection caused by carbapenem-resistant gram-negative bacteria (CRGNB) is a life-threatening complication. This study aimed to assess the current practices and clinical outcomes of intravenous (IV) combined with ...

    Abstract Purpose: Post-neurosurgical intracranial infection caused by carbapenem-resistant gram-negative bacteria (CRGNB) is a life-threatening complication. This study aimed to assess the current practices and clinical outcomes of intravenous (IV) combined with intraventricular (IVT)/intrathecal (ITH) polymyxin B in treating CRGNB intracranial infection.
    Methods: A retrospective study was conducted on patients with post-neurosurgical intracranial infection due to CRGNB from January 2013 to December 2020. Clinical characteristics and treatment outcomes were collected and described. Kaplan-Meier survival and multivariate logistic regression analyses were performed.
    Results: The study included 114 patients, of which 72 received systemic antimicrobial therapy combined with IVT/ITH polymyxin B, and 42 received IV administration alone. Most infections were caused by carbapenem-resistant Acinetobacter baumannii (CRAB, 63.2%), followed by carbapenem-resistant Klebsiella pneumoniae (CRKP, 31.6%). Compared with the IV group, the IVT/ITH group had a higher cerebrospinal fluid (CSF) sterilization rate in 7 days (p < 0.001) and lower 30-day mortality (p = 0.032). In the IVT/ITH group, patients with CRKP infection had a higher initial fever (p = 0.014), higher incidence of bloodstream infection (p = 0.040), lower CSF sterilization in 7 days (p < 0.001), and higher 30-day mortality (p = 0.005) than those with CRAB infection. Multivariate logistic regression analysis revealed that the duration of IVT/ITH polymyxin B (p = 0.021) was independently associated with 30-day mortality.
    Conclusions: Intravenous combined with IVT/ITH polymyxin B increased CSF microbiological eradication and improved clinical outcomes. CRKP intracranial infections may lead to more difficult treatment and thus warrant attention and further optimized treatment.
    Language English
    Publishing date 2024-03-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 603155-9
    ISSN 1435-4373 ; 0934-9723 ; 0722-2211
    ISSN (online) 1435-4373
    ISSN 0934-9723 ; 0722-2211
    DOI 10.1007/s10096-024-04794-y
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1732: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article: Genetic Characteristics and Linkage of Virulence Genes of the

    Zhang, Gensheng / Liu, Wei / Wang, Lin / Ju, Meng / Tian, Xiaxia / Du, Zhimin / Kang, Zhensheng / Zhao, Jie

    Plant disease

    2023  Volume 107, Issue 3, Page(s) 688–700

    Abstract: To understand the inheritance of the TSA- ... ...

    Abstract To understand the inheritance of the TSA-6
    MeSH term(s) Virulence/genetics ; Plant Breeding ; Genotype ; Phenotype ; Basidiomycota/genetics
    Language English
    Publishing date 2023-03-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-07-22-1637-RE
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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