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Article ; Online: A Novel Preanalytical Strategy Enabling Application of a Colorimetric Nanoaptasensor for On-Site Detection of AFB1 in Cattle Feed.

Contreras-Trigo, Braulio / Díaz-García, Víctor / Oyarzún, Patricio

2022 Volume 22, Issue 23

Abstract: Aflatoxin contamination of cattle feed is responsible for serious adverse effects on animal and human health. A number of approaches have been reported to determine aflatoxin B1 (AFB1) in a variety of feed samples using aptasensors. However, rapid ... ...

Abstract	Aflatoxin contamination of cattle feed is responsible for serious adverse effects on animal and human health. A number of approaches have been reported to determine aflatoxin B1 (AFB1) in a variety of feed samples using aptasensors. However, rapid analysis of AFB1 in these matrices remains to be addressed in light of the complexity of the preanalytical process. Herein we describe an optimization on the preanalytical stage to minimize the sample processing steps required to perform semi-quantitative colorimetric detection of AFB1 in cattle feed using a gold nanoparticle-based aptasensor (nano-aptasensor). The optical behavior of the nano-aptasensor was characterized in different organics solvents, with acetonitrile showing the least interference on the activity of the nan-aptasensor. This solvent was selected as the extractant agent for AFB1-containing feed, allowing for the first time, direct colorimetric detection from the crude extract (detection limit of 5 µg/kg). Overall, these results lend support to the application of this technology for the on-site detection of AFB1 in the dairy sector.
Language	English
Publishing date	2022-11-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s22239280
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Article ; Online: Nocturnal seston: A key to explain the cadmium transfer from seawater to mussels (Mytilus chilensis).

Blanc, J Max / Subiabre, Ricardo / Duemler, Jaclyn / Oyarzún, L Camilo / Díaz, Patricio A

Marine pollution bulletin

2023 Volume 195, Page(s) 115544

Abstract: The objective of this work was to discover a biochemical pathway to explain the transfer of cadmium, a toxic element, from seawater to cultured mussels. Understanding the intricacies of this transfer is crucial for global mussel crops, as it has the ... ...

Abstract	The objective of this work was to discover a biochemical pathway to explain the transfer of cadmium, a toxic element, from seawater to cultured mussels. Understanding the intricacies of this transfer is crucial for global mussel crops, as it has the potential to mitigate risks to human health and prevent economic losses in the industry. We focused our investigation on Yal Bay, a typical area with intense mussel aquaculture activity (16,000 t y
MeSH term(s)	Animals ; Humans ; Mytilus/metabolism ; Cadmium/metabolism ; Mytilus edulis ; Aquaculture ; Seawater
Chemical Substances	Cadmium (00BH33GNGH)
Language	English
Publishing date	2023-09-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2001296-2
ISSN	1879-3363 ; 0025-326X
ISSN (online)	1879-3363
ISSN	0025-326X
DOI	10.1016/j.marpolbul.2023.115544
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Article ; Online: A Simple Yet Effective Preanalytical Strategy Enabling the Application of Aptamer-Conjugated Gold Nanoparticles for the Colorimetric Detection of Antibiotic Residues in Raw Milk.

Díaz-García, Víctor / Contreras-Trigo, Braulio / Rodríguez, Camila / Coelho, Pablo / Oyarzún, Patricio

Sensors (Basel, Switzerland)

2022 Volume 22, Issue 3

Abstract: The misuse of antibiotics in the cattle sector can lead to milk contamination, with concomitant effects on the dairy industry and human health. Biosensors can be applied in this field; however, the influence of the milk matrix on their activity has been ... ...

Abstract	The misuse of antibiotics in the cattle sector can lead to milk contamination, with concomitant effects on the dairy industry and human health. Biosensors can be applied in this field; however, the influence of the milk matrix on their activity has been poorly studied in light of the preanalytical process. Herein, aptamer-conjugated gold nanoparticles (nanoaptasensors) were investigated for the colorimetric detection in raw milk of four antibiotics used in cattle. The effect of milk components on the colorimetric response of the nanoaptasensors was analyzed by following the selective aggregation of the nanoparticles, using the absorption ratio A
MeSH term(s)	Animals ; Anti-Bacterial Agents/analysis ; Aptamers, Nucleotide ; Cattle ; Colorimetry ; Gold ; Limit of Detection ; Metal Nanoparticles ; Milk/chemistry
Chemical Substances	Anti-Bacterial Agents ; Aptamers, Nucleotide ; Gold (7440-57-5)
Language	English
Publishing date	2022-02-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s22031281
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Article ; Online: Novel Microsynthesis of High-Yield Gold Nanoparticles to Accelerate Research in Biosensing and Other Bioapplications.

Díaz-García, Víctor / Haensgen, Astrid / Inostroza, Ligia / Contreras-Trigo, Braulio / Oyarzun, Patricio

Biosensors

2023 Volume 13, Issue 12

Abstract: Gold nanoparticles (AuNPs) exhibit unique properties that make them appealing for applications in biosensing and other emerging fields. Despite the availability of numerous synthesis methods, important questions remain to be addressed regarding the ... ...

Abstract	Gold nanoparticles (AuNPs) exhibit unique properties that make them appealing for applications in biosensing and other emerging fields. Despite the availability of numerous synthesis methods, important questions remain to be addressed regarding the volume effect on the synthesis yield and quality of AuNPs in the light of biosensing research. The present study addresses these issues by developing a novel microvolumetric citrate-reduction method to improve the synthesis of AuNPs, which were characterized by electronic microscopy, energy dispersive spectroscopy, zeta potential and colorimetric analysis. A comparison of the novel microsynthesis method with the standard Turkevich method demonstrated its superior performance in terms of yield, monodispersity, rapidity (in one step), reproducibility, and stability. The analytical behavior of AuNPs-based aptasensors prepared by microsynthesis was investigated using kanamycin detection and showed higher reproducibility and improved detection limits (3.4 times) compared to those of Turkevich AuNPs. Finally, the effect of pH was studied to demonstrate the suitability of the method for the screening of AuNP synthesis parameters that are of direct interest in biosensing research; the results showed an optimal pH range between 5.0 and 5.5. In summary, the approach described herein has the potential to improve research capabilities in biosensing, with the added benefits of lowering costs and minimizing waste generation in line with current trends in green nanotechnology.
MeSH term(s)	Gold/chemistry ; Metal Nanoparticles/chemistry ; Reproducibility of Results ; Citric Acid ; Biosensing Techniques/methods
Chemical Substances	Gold (7440-57-5) ; Citric Acid (2968PHW8QP)
Language	English
Publishing date	2023-11-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios13120992
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Article ; Online: An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.

Núñez, Dariela / Cáceres, Rodrigo / Ide, Walther / Varaprasad, Kokkarachedu / Oyarzún, Patricio

International journal of biological macromolecules

2020 Volume 165, Issue Pt B, Page(s) 2711–2720

Abstract: An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell ... ...

Abstract	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock. HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability. The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model. The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
MeSH term(s)	Adsorption/drug effects ; Cellulose/chemistry ; Durapatite/chemistry ; Lead/isolation & purification ; Lead/toxicity ; Nanocomposites/chemistry ; Spectroscopy, Fourier Transform Infrared ; Thermodynamics ; Water/chemistry ; Water Pollutants, Chemical/isolation & purification ; Water Pollutants, Chemical/toxicity ; Water Purification/methods
Chemical Substances	Water Pollutants, Chemical ; Water (059QF0KO0R) ; Lead (2P299V784P) ; Cellulose (9004-34-6) ; Durapatite (91D9GV0Z28)
Keywords	covid19
Language	English
Publishing date	2020-10-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2020.10.055
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Article ; Online: Traqueostomía en niños: Experiencia de 10 años en una Unidad de Cuidados Intensivos Pediátricos.

Oyarzún, Ignacio / Conejero, María José / Adasme, Rodrigo / Pérez, Carolina / Segall, Dafne / Vulletin, Fernando / Oyarzún, María Angélica / Valle, Patricio

Andes pediatrica : revista Chilena de pediatria

2021 Volume 92, Issue 4, Page(s) 511–518

Abstract: Introduction: Pediatric tracheostomy indications have changed over the last 30 years, from acute and transient pro cedures secondary to airway obstruction to programmed tracheostomies indicated due to the need for chronic use of mechanical ventilation ( ... ...

Title translation	Pediatric tracheostomy: Ten year experience in an Intensive Care Unit.
Abstract	Introduction: Pediatric tracheostomy indications have changed over the last 30 years, from acute and transient pro cedures secondary to airway obstruction to programmed tracheostomies indicated due to the need for chronic use of mechanical ventilation (MV). Objective: To describe indications and morbidity associated with pediatric tracheostomies during a ten-year period. Patients and methods: Descrip tive study. Clinical records review of discharged patients (< 15 years old) tracheostomized during their hospital stay between 2005 and 2015. Demographic and clinical variables were evaluated before and after tracheostomy, stay in intensive care unit, age at the time of the tracheostomy, indication of tracheostomy, early complications (< 7 days), late complications (> 7 days), and mortality. Results: 59 children with tracheostomy were analyzed, 36 (59%) tracheostomies were performed in children under 6 months, and 39 (60%) in males. 23 (39%) had a confirmed or under study genopathy and 25 (42%) had congenital heart disease. The main indications for tracheostomy were 58% secondary to airway disease and 42% due to chronic use of MV. Within the airway disease group, subglottic steno sis, vocal cord paralysis, and tracheobronchomalacia were the principal reasons for indication, and in the group of chronic use of MV, the main causes were bronchopulmonary dysplasia and chronic lung disease. We did not find tracheostomy-related mortality. 89% of the patients were discharged with tracheostomy and 59% with chronic use of MV. The probability of being discharged with a tracheos tomy was higher in younger patients while the chronic use of MV at discharge was higher in patients with a greater number of extubation failures before tracheostomy. Conclusion: Tracheostomy is a safe procedure in children, where the predominant causes of indication are airway disease and the need for chronic use of MV. Most children with tracheostomies are discharged with tracheostomy and chronic use of MV. Younger children, those with difficult weaning, confirmed or suspected ge nopathy, or special health needs are at greater risk of needing tracheostomy and chronic use of MV.
MeSH term(s)	Adolescent ; Child ; Female ; Humans ; Infant, Newborn ; Intensive Care Units, Pediatric ; Length of Stay ; Male ; Respiration, Artificial ; Retrospective Studies ; Tracheobronchomalacia/epidemiology ; Tracheostomy/adverse effects ; Vocal Cord Paralysis/epidemiology
Language	Spanish
Publishing date	2021-10-15
Publishing country	Chile
Document type	Journal Article
ISSN	2452-6053
ISSN (online)	2452-6053
DOI	10.32641/andespediatr.v92i4.2667
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Article ; Online: Recombinant and epitope-based vaccines on the road to the market and implications for vaccine design and production.

Oyarzún, Patricio / Kobe, Bostjan

Human vaccines & immunotherapeutics

2016 Volume 12, Issue 3, Page(s) 763–767

Abstract: Novel vaccination approaches based on rational design of B- and T-cell epitopes - epitope-based vaccines - are making progress in the clinical trial pipeline. The epitope-focused recombinant protein-based malaria vaccine (termed RTS,S) is a next- ... ...

Abstract	Novel vaccination approaches based on rational design of B- and T-cell epitopes - epitope-based vaccines - are making progress in the clinical trial pipeline. The epitope-focused recombinant protein-based malaria vaccine (termed RTS,S) is a next-generation approach that successfully reached phase-III trials, and will potentially become the first commercial vaccine against a human parasitic disease. Progress made on methods such as recombinant DNA technology, advanced cell-culture techniques, immunoinformatics and rational design of immunogens are driving the development of these novel concepts. Synthetic recombinant proteins comprising both B- and T-cell epitopes can be efficiently produced through modern biotechnology and bioprocessing methods, and can enable the induction of large repertoires of immune specificities. In particular, the inclusion of appropriate CD4+ T-cell epitopes is increasingly considered a key vaccine component to elicit robust immune responses, as suggested by results coming from HIV-1 clinical trials. In silico strategies for vaccine design are under active development to address genetic variation in pathogens and several broadly protective "universal" influenza and HIV-1 vaccines are currently at different stages of clinical trials. Other methods focus on improving population coverage in target populations by rationally considering specificity and prevalence of the HLA proteins, though a proof-of-concept in humans has not been demonstrated yet. Overall, we expect immunoinformatics and bioprocessing methods to become a central part of the next-generation epitope-based vaccine development and production process.
MeSH term(s)	Clinical Trials as Topic ; Drug Discovery/methods ; Drug Discovery/trends ; Epitopes/genetics ; Epitopes/immunology ; Humans ; Vaccines, Synthetic/genetics ; Vaccines, Synthetic/immunology
Chemical Substances	Epitopes ; Vaccines, Synthetic
Language	English
Publishing date	2016--03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2664176-8
ISSN	2164-554X ; 2164-5515
ISSN (online)	2164-554X
ISSN	2164-5515
DOI	10.1080/21645515.2015.1094595
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Article ; Online: Factors Associated With Accidental Decannulation in Tracheostomized Children.

Villarroel, Gregory S / Faúndez, Macarena / Jalil, Yorschua F / Oyarzún, Ignacio J / Fernandez, Tiziana R / Barañao, Patricio I / Mendez, Mireya P / Muñoz, Sergio R

Respiratory care

2022 Volume 68, Issue 2, Page(s) 173–179

Abstract: Background: Tracheostomy has many benefits for pediatric patients in the ICU, but it is also associated with complications. Accidental decannulation (AD) is a frequent complication and cause of mortality in this population. Our study aimed to determine ... ...

Abstract	Background: Tracheostomy has many benefits for pediatric patients in the ICU, but it is also associated with complications. Accidental decannulation (AD) is a frequent complication and cause of mortality in this population. Our study aimed to determine the factors associated with AD in tracheostomized pediatric subjects. Methods: This was a case-control study with 1:2 allocation ratio. Participants were tracheostomized children hospitalized in a prolonged mechanical ventilation hospital between 2013-2018. Each child who experienced decannulation during the study period was included as a case at the time of the event. Controls were obtained from the same population and were defined as subjects without an AD event during the same period. Results: One hundred forty patients were hospitalized at Josefina Martinez Hospital at the time, of whom 41 were selected as cases and 82 as controls. Median (interquartile range) age was 20 (12-36) months, being 60% male. The median time from tracheostomy placement to AD event was 364 (167-731) d. Eighty-four percent of subjects were mechanically ventilated. AD mainly occurred by self-decannulation (53.7%). The risk of AD was higher in children who reached the midline in a sitting position (odds ratio 9.5 [95% CI 1.59-53.90]), inner diameter (ID) tracheostomy tube size ≤ 4.0 mm (odds ratio 5.18 [95% CI 1.41-19.06]), and who had been hospitalized in hospital rooms with a low ratio of nursing staff for each subject (1 nurse to 4 subjects) (odds ratio 4.48 [95% CI 1.19-16.80]). Conclusions: Factors associated with a higher risk of AD in tracheostomized children included the ability to reach the midline in a sitting position, the use of a smaller tracheostomy tube (≤ 4.0 mm ID), and lower supervision from staff.
MeSH term(s)	Humans ; Child ; Male ; Infant ; Female ; Case-Control Studies ; Hospitals ; Nursing Staff ; Odds Ratio ; Respiration, Artificial
Language	English
Publishing date	2022-12-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	603252-7
ISSN	1943-3654 ; 0098-9142 ; 0020-1324
ISSN (online)	1943-3654
ISSN	0098-9142 ; 0020-1324
DOI	10.4187/respcare.09673
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Article ; Online: Nuclear accumulation of β-catenin is associated with endosomal sequestration of the destruction complex and increased activation of Rab5 in oral dysplasia.

Reyes, Montserrat / Peña-Oyarzún, Daniel / Silva, Patricio / Venegas, Sebastián / Criollo, Alfredo / Torres, Vicente A

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

2020 Volume 34, Issue 3, Page(s) 4009–4025

Abstract: Potentially malignant lesions, commonly referred to as dysplasia, are associated with malignant transformation by mechanisms that remain unclear. We recently reported that increased Wnt secretion promotes the nuclear accumulation of β-catenin and ... ...

Abstract	Potentially malignant lesions, commonly referred to as dysplasia, are associated with malignant transformation by mechanisms that remain unclear. We recently reported that increased Wnt secretion promotes the nuclear accumulation of β-catenin and expression of target genes in oral dysplasia. However, the mechanisms accounting for nuclear re-localization of β-catenin in oral dysplasia remain unclear. In this study, we show that endosomal sequestration of the β-catenin destruction complex allows nuclear accumulation of β-catenin in oral dysplasia, and that these events depended on the endocytic protein Rab5. Tissue immunofluorescence analysis showed aberrant accumulation of enlarged early endosomes in oral dysplasia biopsies, when compared with healthy oral mucosa. These observations were confirmed in cell culture models, by comparing dysplastic oral keratinocytes (DOK) and non-dysplastic oral keratinocytes (OKF6). Intriguingly, DOK depicted higher levels of active Rab5, a critical regulator of early endosomes, when compared with OKF6. Increased Rab5 activity in DOK was necessary for nuclear localization of β-catenin and Tcf/Lef-dependent transcription, as shown by expression of dominant negative and constitutively active mutants of Rab5, along with immunofluorescence, subcellular fractionation, transcription, and protease protection assays. Mechanistically, elevated Rab5 activity in DOK accounted for endosomal sequestration of components of the destruction complex, including GSK3β, Axin, and adenomatous polyposis coli (APC), as observed in Rab5 dominant negative experiments. In agreement with these in vitro observations, tissue immunofluorescence analysis showed increased co-localization of GSK3β, APC, and Axin, with early endosome antigen 1- and Rab5-positive early endosomes in clinical samples of oral dysplasia. Collectively, these data indicate that increased Rab5 activity and endosomal sequestration of the β-catenin destruction complex leads to stabilization and nuclear accumulation of β-catenin in oral dysplasia.
MeSH term(s)	Apraxias/metabolism ; Cell Line ; Cell Nucleus/metabolism ; Endosomes/metabolism ; Fluorescent Antibody Technique ; Humans ; beta Catenin/metabolism ; rab GTP-Binding Proteins/metabolism ; rab5 GTP-Binding Proteins/metabolism
Chemical Substances	beta Catenin ; rab7 protein (152989-05-4) ; rab11 protein (EC 3.6.1.-) ; rab GTP-Binding Proteins (EC 3.6.5.2) ; rab5 GTP-Binding Proteins (EC 3.6.5.2)
Language	English
Publishing date	2020-01-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639186-2
ISSN	1530-6860 ; 0892-6638
ISSN (online)	1530-6860
ISSN	0892-6638
DOI	10.1096/fj.201902345RR
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Zs.A 2266: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water

Núñez, Dariela / Cáceres, Rodrigo / Ide, Walther / Varaprasad, Kokkarachedu / Oyarzún, Patricio

Int. j. biol. macromol

Abstract	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock. HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability. The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model. The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192â¯mg/g) and packed-bed column systems (188â¯mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #33069824
Database	COVID19

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