Result 1 - 10 of total 669
Epirrhosis : Festgabe für Carl Schmitt Vol. 1 , p. 333-343
1968 Volume 1, Page(s) 333–343
| Author's details | Arnold Schmitz |
|---|---|
| Publisher | Duncker & Humblot |
| Publishing place | Berlin |
| Document type | Article |
| Database | Former special subject collection: coastal and deep sea fishing |
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Untersuchungen zur Sulfatierung, Struktur und Synthese eines Membranproteins von Thyreocyten
1992
| Author's details | vorgelegt von Anton Schmitz |
|---|---|
| Language | German |
| Size | 89 S. : Ill., graph. Darst. |
| Document type | Book ; Thesis |
| Thesis / German Habilitation thesis | München, Univ., Diss., 1992 |
| HBZ-ID | HT006353936 |
| Database | Catalogue ZB MED Medicine, Health |
| Shelf mark | Loan status | Location |
|---|---|---|
| 94 D 2681 | order for loan | Magazin |
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2023 Volume 13, Issue 3
Abstract: Human papillomavirus (HPV) infection is the leading cause of cervical cancer, and vaccination with HPV L1 capsid proteins has been successful in controlling it. However, vaccination coverage is not universal, particularly in developing countries, where ... ...
| Abstract | Human papillomavirus (HPV) infection is the leading cause of cervical cancer, and vaccination with HPV L1 capsid proteins has been successful in controlling it. However, vaccination coverage is not universal, particularly in developing countries, where 80% of all cervical cancer cases occur. Cost-effective vaccination could be achieved by expressing the L1 protein in plants. Various efforts have been made to produce the L1 protein in plants, including attempts to express it in chloroplasts for high-yield performance. However, manipulating chloroplast gene expression requires complex and difficult-to-control expression elements. In recent years, a family of nuclear-encoded, chloroplast-targeted RNA-binding proteins, the pentatricopeptide repeat (PPR) proteins, were described as key regulators of chloroplast gene expression. For example, PPR proteins are used by plants to stabilize and translate chloroplast mRNAs. The objective is to demonstrate that a PPR target site can be used to drive HPV L1 expression in chloroplasts. To test our hypothesis, we used biolistic chloroplast transformation to establish tobacco lines that express two variants of the HPV L1 protein under the control of the target site of the PPR protein CHLORORESPIRATORY REDUCTION2 (CRR2). The transgenes were inserted into a dicistronic operon driven by the plastid rRNA promoter. To determine the effectiveness of the PPR target site for the expression of the HPV L1 protein in the chloroplasts, we analyzed the accumulation of the transgenic mRNA and its processing, as well as the accumulation of the L1 protein in the transgenic lines. We established homoplastomic lines carrying either the HPV18 L1 protein or an HPV16B Enterotoxin::L1 fusion protein. The latter line showed severe growth retardation and pigment loss, suggesting that the fusion protein is toxic to the chloroplasts. Despite the presence of dicistronic mRNAs, we observed very little accumulation of monocistronic transgenic mRNA and no significant increase in CRR2-associated small RNAs. Although both lines expressed the L1 protein, quantification using an external standard suggested that the amounts were low. Our results suggest that PPR binding sites can be used to drive vaccine expression in plant chloroplasts; however, the factors that modulate the effectiveness of target gene expression remain unclear. The identification of dozens of PPR binding sites through small RNA sequencing expands the set of expression elements available for high-value protein production in chloroplasts. |
|---|---|
| Language | English |
| Publishing date | 2023-02-21 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2662251-8 |
| ISSN | 2218-1989 |
| ISSN | 2218-1989 |
| DOI | 10.3390/metabo13030315 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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German medical science : GMS e-journal
2023 Volume 21, Page(s) Doc11
| Title translation | Specialist clinics - definition: proposal for the discussion from the ad hoc committee on care structures by the Association of the Scientific Medical Societies in Germany (AWMF) for the reform of hospital care. |
|---|---|
| MeSH term(s) | Humans ; Germany ; Hospitals |
| Language | German |
| Publishing date | 2023-07-05 |
| Publishing country | Germany |
| Document type | Journal Article ; Review |
| ZDB-ID | 2113606-3 |
| ISSN | 1612-3174 ; 1612-3174 |
| ISSN (online) | 1612-3174 |
| ISSN | 1612-3174 |
| DOI | 10.3205/000325 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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2023 Volume 111, Issue 19, Page(s) 3041–3052.e7
Abstract: The persistence of play after decortication points to a subcortical mechanism of play control. We found that global blockade of the rat periaqueductal gray with either muscimol or lidocaine interfered with ticklishness and play. We recorded vocalizations ...
| Abstract | The persistence of play after decortication points to a subcortical mechanism of play control. We found that global blockade of the rat periaqueductal gray with either muscimol or lidocaine interfered with ticklishness and play. We recorded vocalizations and neural activity from the periaqueductal gray of young, playful rats during interspecific touch, play, and tickling. Rats vocalized weakly to touch and more strongly to play and tickling. Periaqueductal gray units showed diverse but strong modulation to tickling and play. Hierarchical clustering based on neuronal responses to play and tickling revealed functional clusters mapping to different periaqueductal gray columns. Specifically, we observed play-neutral/tickling-inhibited and tickling/play-neutral units in dorsolateral and dorsomedial periaqueductal gray columns. In contrast, strongly play/tickling-excited units mapped to the lateral columns and were suppressed by anxiogenic conditions. Optogenetic inactivation of lateral periaqueductal columns disrupted ticklishness and play. We conclude that the lateral periaqueductal gray columns are decisive for play and laughter. |
|---|---|
| MeSH term(s) | Rats ; Animals ; Periaqueductal Gray/physiology ; Touch/physiology ; Neurons/physiology ; Touch Perception |
| Language | English |
| Publishing date | 2023-07-28 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 808167-0 |
| ISSN | 1097-4199 ; 0896-6273 |
| ISSN (online) | 1097-4199 |
| ISSN | 0896-6273 |
| DOI | 10.1016/j.neuron.2023.06.018 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 2496: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
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| Zs.MG 92: Show issues |
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2024 Volume 14, Page(s) 1240104
Abstract: Context: The condition when a person's gender identity does not match the sex assigned at birth is called gender incongruence (GI). Numbers of GI people seeking medical care increased tremendously over the last decade. Diabetes mellitus is a severe and ... ...
| Abstract | Context: The condition when a person's gender identity does not match the sex assigned at birth is called gender incongruence (GI). Numbers of GI people seeking medical care increased tremendously over the last decade. Diabetes mellitus is a severe and lifelong disease. GI combined with diabetes may potentiate into a burdensome package for affected people. Objective: The study aimed to characterize people with GI and diabetes from an extensive standardized registry, the Prospective Diabetes Follow-up Registry (DPV), and to identify potential metabolic and psychological burdens. Methods: We compared demographic and clinical registry data of persons with type 1 or type 2 diabetes and GI to those without GI and used propensity score matching (1:4) with age, diabetes duration and treatment year as covariates. Results: 75 persons with GI, 49 with type 1 and 26 with type 2 diabetes were identified. HbA1c values were similar in matched persons with type 1 or 2 diabetes and GI compared to those without GI. Lipid profiles showed no difference, neither in type 1 nor in type 2 diabetes. Diastolic blood pressure was higher in the type 1 and GI group than in those without, whereas systolic blood pressure showed comparable results in all groups. Depression and anxiety were significantly higher in GI people (type 1 and 2). Non-suicidal self-injurious behaviour was more common in type 1 and GI, as was suicidality in type 2 with GI. Conclusion: Mental health issues are frequent in people with diabetes and GI and need to be specially addressed in this population. |
|---|---|
| MeSH term(s) | Infant, Newborn ; Humans ; Male ; Female ; Diabetes Mellitus, Type 2/epidemiology ; Mental Health ; Prospective Studies ; Gender Identity ; Registries |
| Language | English |
| Publishing date | 2024-01-22 |
| Publishing country | Switzerland |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2592084-4 |
| ISSN | 1664-2392 |
| ISSN | 1664-2392 |
| DOI | 10.3389/fendo.2023.1240104 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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medRxiv : the preprint server for health sciences
2024
Abstract: Objective: To evaluate whether there is an enrichment of rare variants in familial hemophagocytic lymphohistiocytosis (HLH) genes and systemic juvenile idiopathic arthritis (sJIA) with or without macrophage activation syndrome (MAS).: Methods: ... ...
| Abstract | Objective: To evaluate whether there is an enrichment of rare variants in familial hemophagocytic lymphohistiocytosis (HLH) genes and systemic juvenile idiopathic arthritis (sJIA) with or without macrophage activation syndrome (MAS). Methods: Targeted sequencing of HLH genes ( Results: Sequencing data from 524 sJIA cases were jointly called and harmonized with exome-derived target data from 3000 controls. Quality control operations produced a set of 481 cases and 2924 ancestrally-matched control subjects. RVT of sJIA cases and controls revealed a significant association with rare protein-altering variants (minor allele frequency [MAF]<0.01) of Conclusion: We identified an enrichment of rare HLH variants in sJIA patients compared with healthy controls, driven by |
|---|---|
| Language | English |
| Publishing date | 2024-03-15 |
| Publishing country | United States |
| Document type | Preprint |
| DOI | 10.1101/2024.03.13.24304215 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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Angewandte Chemie (Weinheim an der Bergstrasse, Germany)
2021 Volume 133, Issue 18, Page(s) 10367–10373
Abstract: The receptor binding domain (RBD) of the spike glycoprotein of the coronavirus SARS-CoV-2 (CoV2-S) binds to the human angiotensin-converting enzyme 2 (ACE2) representing the initial contact point for leveraging the infection cascade. We used an automated ...
| Abstract | The receptor binding domain (RBD) of the spike glycoprotein of the coronavirus SARS-CoV-2 (CoV2-S) binds to the human angiotensin-converting enzyme 2 (ACE2) representing the initial contact point for leveraging the infection cascade. We used an automated selection process and identified an aptamer that specifically interacts with CoV2-S. The aptamer does not bind to the RBD of CoV2-S and does not block the interaction of CoV2-S with ACE2. Nevertheless, infection studies revealed potent and specific inhibition of pseudoviral infection by the aptamer. The present study opens up new vistas in developing SARS-CoV2 infection inhibitors, independent of blocking the ACE2 interaction of the virus, and harnesses aptamers as potential drug candidates and tools to disentangle hitherto inaccessible infection modalities, which is of particular interest in light of the increasing number of escape mutants that are currently being reported. |
|---|---|
| Language | English |
| Publishing date | 2021-03-25 |
| Publishing country | Germany |
| Document type | Journal Article |
| ZDB-ID | 506609-8 |
| ISSN | 1521-3757 ; 0044-8249 ; 0932-2140 |
| ISSN (online) | 1521-3757 |
| ISSN | 0044-8249 ; 0932-2140 |
| DOI | 10.1002/ange.202100316 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Ui VI 2770: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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Angewandte Chemie (International ed. in English)
2021 Volume 60, Issue 18, Page(s) 10279–10285
Abstract: The receptor binding domain (RBD) of the spike glycoprotein of the coronavirus SARS-CoV-2 (CoV2-S) binds to the human angiotensin-converting enzyme 2 (ACE2) representing the initial contact point for leveraging the infection cascade. We used an automated ...
| Abstract | The receptor binding domain (RBD) of the spike glycoprotein of the coronavirus SARS-CoV-2 (CoV2-S) binds to the human angiotensin-converting enzyme 2 (ACE2) representing the initial contact point for leveraging the infection cascade. We used an automated selection process and identified an aptamer that specifically interacts with CoV2-S. The aptamer does not bind to the RBD of CoV2-S and does not block the interaction of CoV2-S with ACE2. Nevertheless, infection studies revealed potent and specific inhibition of pseudoviral infection by the aptamer. The present study opens up new vistas in developing SARS-CoV2 infection inhibitors, independent of blocking the ACE2 interaction of the virus, and harnesses aptamers as potential drug candidates and tools to disentangle hitherto inaccessible infection modalities, which is of particular interest in light of the increasing number of escape mutants that are currently being reported. |
|---|---|
| MeSH term(s) | Angiotensin-Converting Enzyme 2/metabolism ; Antiviral Agents/chemistry ; Antiviral Agents/pharmacology ; Aptamers, Nucleotide/chemistry ; Aptamers, Nucleotide/pharmacology ; Binding Sites/drug effects ; COVID-19/metabolism ; Drug Discovery ; HEK293 Cells ; Humans ; Protein Binding/drug effects ; Protein Interaction Domains and Motifs/drug effects ; SARS-CoV-2/chemistry ; SARS-CoV-2/drug effects ; SARS-CoV-2/physiology ; SELEX Aptamer Technique ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/metabolism ; COVID-19 Drug Treatment |
| Chemical Substances | Antiviral Agents ; Aptamers, Nucleotide ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; ACE2 protein, human (EC 3.4.17.23) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) |
| Language | English |
| Publishing date | 2021-03-23 |
| Publishing country | Germany |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2011836-3 |
| ISSN | 1521-3773 ; 1433-7851 |
| ISSN (online) | 1521-3773 |
| ISSN | 1433-7851 |
| DOI | 10.1002/anie.202100316 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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1992
| Author's details | Karl Schmitz-Moormann |
|---|---|
| Keywords | Alcoholism / in old age ; Alter ; Alkoholkonsum |
| Subject | Alkoholgenuss ; Alkoholverbrauch ; Alkohol ; Alter Mensch ; Betagter ; Senioren ; Senior |
| Language | German |
| Size | 62 S. : graph. Darst. |
| Publisher | Neuland |
| Publishing place | Geesthacht |
| Document type | Book |
| HBZ-ID | HT004276992 |
| ISBN | 3-87581-099-6 ; 978-3-87581-099-8 |
| Database | Catalogue ZB MED Medicine, Health |
| Shelf mark | Loan status | Location |
|---|---|---|
| 1997 A 9094 | order for loan | Magazin |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.