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  1. Article: Enzymatic properties of CARF-domain proteins in

    Ding, Jin / Schuergers, Nils / Baehre, Heike / Wilde, Annegret

    Frontiers in microbiology

    2022  Volume 13, Page(s) 1046388

    Abstract: Prokaryotic CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated genes) systems provide immunity against invading genetic elements such as bacteriophages and plasmids. In type III CRISPR systems, the recognition of ... ...

    Abstract Prokaryotic CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated genes) systems provide immunity against invading genetic elements such as bacteriophages and plasmids. In type III CRISPR systems, the recognition of target RNA leads to the synthesis of cyclic oligoadenylate (cOA) second messengers that activate ancillary effector proteins
    Language English
    Publishing date 2022-11-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.1046388
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: PATAN-domain regulators interact with the Type IV pilus motor to control phototactic orientation in the cyanobacterium Synechocystis.

    Han, Yu / Jakob, Annik / Engel, Sophia / Wilde, Annegret / Schuergers, Nils

    Molecular microbiology

    2022  Volume 117, Issue 4, Page(s) 790–801

    Abstract: ... where they interact with the N-terminal cytoplasmic domain of PilC and the pilus assembly ATPase PilB1. By separately ... expressing the subdomains of the response regulator PixE, we confirm that only the N-terminal PATAN domain ...

    Abstract Many prokaryotes show complex behaviors that require the intricate spatial and temporal organization of cellular protein machineries, leading to asymmetrical protein distribution and cell polarity. One such behavior is cyanobacterial phototaxis which relies on the dynamic localization of the Type IV pilus motor proteins in response to light. In the cyanobacterium Synechocystis, various signaling systems encompassing chemotaxis-related CheY- and PatA-like response regulators are critical players in switching between positive and negative phototaxis depending on the light intensity and wavelength. In this study, we show that PatA-type regulators evolved from chemosensory systems. Using fluorescence microscopy and yeast two-hybrid analysis, we demonstrate that they localize to the inner membrane, where they interact with the N-terminal cytoplasmic domain of PilC and the pilus assembly ATPase PilB1. By separately expressing the subdomains of the response regulator PixE, we confirm that only the N-terminal PATAN domain interacts with PilB1, localizes to the membrane, and is sufficient to reverse phototactic orientation. These experiments established that the PATAN domain is the principal output domain of PatA-type regulators which we presume to modulate pilus extension by binding to the pilus motor components.
    MeSH term(s) Adenosine Triphosphatases/metabolism ; Bacterial Proteins/metabolism ; Fimbriae, Bacterial/metabolism ; Phototaxis ; Synechocystis/metabolism
    Chemical Substances Bacterial Proteins ; Adenosine Triphosphatases (EC 3.6.1.-)
    Language English
    Publishing date 2022-01-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/mmi.14872
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online ; Thesis: Funktion und Lokalisation von Hfq in Synechocystis sp. PCC6803

    Schürgers, Nils

    2014  

    Title variant Function and localization of Hfq in Synechocystis sp. PCC6803
    Author's details Nils Schürgers
    Language German
    Size Online-Ressource
    Publisher Universitätsbibliothek
    Publishing place Gießen
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Gießen, Justus-Liebig-Universität, Diss., 2014
    Database Former special subject collection: coastal and deep sea fishing

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  4. Article: Appendages of the cyanobacterial cell.

    Schuergers, Nils / Wilde, Annegret

    Life (Basel, Switzerland)

    2015  Volume 5, Issue 1, Page(s) 700–715

    Abstract: Extracellular non-flagellar appendages, called pili or fimbriae, are widespread in gram-negative bacteria. They are involved in many different functions, including motility, adhesion, biofilm formation, and uptake of DNA. Sequencing data for a large ... ...

    Abstract Extracellular non-flagellar appendages, called pili or fimbriae, are widespread in gram-negative bacteria. They are involved in many different functions, including motility, adhesion, biofilm formation, and uptake of DNA. Sequencing data for a large number of cyanobacterial genomes revealed that most of them contain genes for pili synthesis. However, only for a very few cyanobacteria structure and function of these appendages have been analyzed. Here, we review the structure and function of type IV pili in Synechocystis sp. PCC 6803 and analyze the distribution of type IV pili associated genes in other cyanobacteria. Further, we discuss the role of the RNA-chaperone Hfq in pilus function and the presence of genes for the chaperone-usher pathway of pilus assembly in cyanobacteria.
    Language English
    Publishing date 2015-03-04
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life5010700
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  5. Article ; Online: Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring.

    Zubkovs, Vitalijs / Wang, Hanxuan / Schuergers, Nils / Weninger, Astrid / Glieder, Anton / Cattaneo, Stefano / Boghossian, Ardemis A

    Nanoscale advances

    2022  Volume 4, Issue 11, Page(s) 2420–2427

    Abstract: Single-walled carbon nanotubes (SWCNTs) emit photostable near-infrared (NIR) fluorescence that is conducive for optical glucose monitoring. Such SWCNT-based optical sensors often require the immobilization of proteins that can confer glucose selectivity ... ...

    Abstract Single-walled carbon nanotubes (SWCNTs) emit photostable near-infrared (NIR) fluorescence that is conducive for optical glucose monitoring. Such SWCNT-based optical sensors often require the immobilization of proteins that can confer glucose selectivity and reactivity. In this work, we immobilize a glucose-reactive enzyme, glucose oxidase (GOx), onto SWCNTs using a
    Language English
    Publishing date 2022-05-04
    Publishing country England
    Document type Journal Article
    ISSN 2516-0230
    ISSN (online) 2516-0230
    DOI 10.1039/d2na00092j
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Phototaxis Assays of

    Jakob, Annik / Schuergers, Nils / Wilde, Annegret

    Bio-protocol

    2017  Volume 7, Issue 11, Page(s) e2328

    Abstract: Phototaxis is a mechanism that allows cyanobacteria to respond to fluctuations in the quality and quantity of illumination by moving either towards or away from a light source. Phototactic movement on low concentration agar or agarose plates can be ... ...

    Abstract Phototaxis is a mechanism that allows cyanobacteria to respond to fluctuations in the quality and quantity of illumination by moving either towards or away from a light source. Phototactic movement on low concentration agar or agarose plates can be analyzed at macroscopic and microscopic scales representing group behavior and single cell motility, respectively. Here, we describe a detailed procedure for phototaxis assays on both scales using the unicellular cyanobacterium
    Language English
    Publishing date 2017-06-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.2328
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Cyanobacteria in motion.

    Schuergers, Nils / Mullineaux, Conrad W / Wilde, Annegret

    Current opinion in plant biology

    2017  Volume 37, Page(s) 109–115

    Abstract: Cyanobacteria are able to move directly towards or away from a light source, a process called phototaxis. Recent studies have revealed that the spherical unicellular cyanobacterium Synechocystis sp. PCC 6803 exhibits a cell polarity in response to ... ...

    Abstract Cyanobacteria are able to move directly towards or away from a light source, a process called phototaxis. Recent studies have revealed that the spherical unicellular cyanobacterium Synechocystis sp. PCC 6803 exhibits a cell polarity in response to unidirectional illumination and that micro-optic properties of cyanobacterial cells are the basis of their directional light sensing. Further functional and physiological studies highlight a very complex control of cyanobacterial phototaxis by sensory proteins, histidine kinases and response regulators. Notably, PATAN domain response regulators appear to participate in directional control of phototaxis in the cyanobacterium Synechocystis sp. PCC 6803. In this review we explain the problem of directional light sensing at the small scale of bacteria and discuss our current understanding of signal transduction in cyanobacterial phototaxis.
    Language English
    Publishing date 2017-06
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1418472-2
    ISSN 1879-0356 ; 1369-5266
    ISSN (online) 1879-0356
    ISSN 1369-5266
    DOI 10.1016/j.pbi.2017.03.018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Cyanobacteria in motion

    Schuergers, Nils / Mullineaux, Conrad W / Wilde, Annegret

    Current opinion in plant biology. 2017 June, v. 37

    2017  

    Abstract: Cyanobacteria are able to move directly towards or away from a light source, a process called phototaxis. Recent studies have revealed that the spherical unicellular cyanobacterium Synechocystis sp. PCC 6803 exhibits a cell polarity in response to ... ...

    Abstract Cyanobacteria are able to move directly towards or away from a light source, a process called phototaxis. Recent studies have revealed that the spherical unicellular cyanobacterium Synechocystis sp. PCC 6803 exhibits a cell polarity in response to unidirectional illumination and that micro-optic properties of cyanobacterial cells are the basis of their directional light sensing. Further functional and physiological studies highlight a very complex control of cyanobacterial phototaxis by sensory proteins, histidine kinases and response regulators. Notably, PATAN domain response regulators appear to participate in directional control of phototaxis in the cyanobacterium Synechocystis sp. PCC 6803. In this review we explain the problem of directional light sensing at the small scale of bacteria and discuss our current understanding of signal transduction in cyanobacterial phototaxis.
    Keywords bacteria ; cell polarity ; histidine kinase ; lighting ; phototaxis ; proteins ; signal transduction ; Synechocystis sp. PCC 6714 ; Synechocystis sp. PCC 6803
    Language English
    Dates of publication 2017-06
    Size p. 109-115.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1418472-2
    ISSN 1879-0356 ; 1369-5266
    ISSN (online) 1879-0356
    ISSN 1369-5266
    DOI 10.1016/j.pbi.2017.03.018
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: A Synthetic Biology Approach to Engineering Living Photovoltaics.

    Schuergers, N / Werlang, C / Ajo-Franklin, C M / Boghossian, A A

    Energy & environmental science

    2017  Volume 10, Issue 5, Page(s) 1102–1115

    Abstract: The ability to electronically interface living cells with electron accepting scaffolds is crucial for the development of next-generation biophotovoltaic technologies. Although recent studies have focused on engineering synthetic interfaces that can ... ...

    Abstract The ability to electronically interface living cells with electron accepting scaffolds is crucial for the development of next-generation biophotovoltaic technologies. Although recent studies have focused on engineering synthetic interfaces that can maximize electronic communication between the cell and scaffold, the efficiency of such devices is limited by the low conductivity of the cell membrane. This review provides a materials science perspective on applying a complementary, synthetic biology approach to engineering membrane-electrode interfaces. It focuses on the technical challenges behind the introduction of foreign extracellular electron transfer pathways in bacterial host cells and the past and future efforts to engineer photosynthetic organisms with artificial electron-export capabilities for biophotovoltaic applications. The article highlights advances in engineering protein-based, electron-exporting conduits in a model host organism, E. coli, before reviewing state-of-the-art biophotovoltaic technologies that use both unmodified and bioengineered photosynthetic bacteria with improved electron transport capabilities. A thermodynamic analysis is used to propose an energetically feasible pathway for extracellular electron transport in engineered cyanobacteria and identify metabolic bottlenecks amenable to protein engineering techniques. Based on this analysis, an engineered photosynthetic organism expressing a foreign, protein-based electron conduit yields a maximum theoretical solar conversion efficiency of 6-10% without accounting for additional bioengineering optimizations for light-harvesting.
    Language English
    Publishing date 2017-04-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2439879-2
    ISSN 1754-5706 ; 1754-5692
    ISSN (online) 1754-5706
    ISSN 1754-5692
    DOI 10.1039/C7EE00282C
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Development of a Highly Sensitive Luciferase-Based Reporter System To Study Two-Step Protein Secretion in Cyanobacteria.

    Russo, David A / Zedler, Julie A Z / Conradi, Fabian D / Schuergers, Nils / Jensen, Poul Erik / Mullineaux, Conrad W / Wilde, Annegret / Pohnert, Georg

    Journal of bacteriology

    2021  Volume 204, Issue 2, Page(s) e0050421

    Abstract: Cyanobacteria, ubiquitous oxygenic photosynthetic bacteria, interact with the environment and their surrounding microbiome through the secretion of a variety of small molecules and proteins. The release of these compounds is mediated by sophisticated ... ...

    Abstract Cyanobacteria, ubiquitous oxygenic photosynthetic bacteria, interact with the environment and their surrounding microbiome through the secretion of a variety of small molecules and proteins. The release of these compounds is mediated by sophisticated multiprotein complexes, also known as secretion systems. Genomic analyses indicate that protein and metabolite secretion systems are widely found in cyanobacteria; however, little is known regarding their function, regulation, and secreted effectors. One such system, the type IVa pilus system (T4aPS), is responsible for the assembly of dynamic cell surface appendages, type IVa pili (T4aP), that mediate ecologically relevant processes such as phototactic motility, natural competence, and adhesion. Several studies have suggested that the T4aPS can also act as a two-step protein secretion system in cyanobacteria akin to the homologous type II secretion system in heterotrophic bacteria. To determine whether the T4aP are involved in two-step secretion of nonpilin proteins, we developed a NanoLuc (NLuc)-based quantitative secretion reporter for the model cyanobacterium
    MeSH term(s) Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biological Assay/methods ; Fimbriae, Bacterial ; Luciferases/metabolism ; Protein Translocation Systems/genetics ; Protein Translocation Systems/metabolism ; Protein Transport ; Synechocystis/genetics ; Synechocystis/metabolism
    Chemical Substances Bacterial Proteins ; Protein Translocation Systems ; Luciferases (EC 1.13.12.-) ; nanoluc (EC 1.13.12.-)
    Language English
    Publishing date 2021-12-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/JB.00504-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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