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  1. Article ; Online: Antigenic imprinting in SARS-CoV-2.

    Reincke, S Momsen / Prüss, Harald / Wilson, Ian A / Kreye, Jakob

    Clinical and translational medicine

    2022  Volume 12, Issue 7, Page(s) e923

    MeSH term(s) Antibodies, Viral ; COVID-19 ; Humans ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus
    Chemical Substances Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-07-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2697013-2
    ISSN 2001-1326 ; 2001-1326
    ISSN (online) 2001-1326
    ISSN 2001-1326
    DOI 10.1002/ctm2.923
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Online ; Thesis: Repertoireanalyse monoklonaler Antikörper des Liquors von Patienten mit anti-NMDA-Rezeptor-Enzephalitis

    Kreye, Jakob [Verfasser]

    2017  

    Author's details Jakob Kreye
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Medizinische Fakultät Charité - Universitätsmedizin Berlin
    Publishing place Berlin
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  3. Article ; Online: Antigenic imprinting in SARS‐CoV‐2

    S. Momsen Reincke / Harald Prüss / Ian A. Wilson / Jakob Kreye

    Clinical and Translational Medicine, Vol 12, Iss 7, Pp n/a-n/a (2022)

    2022  

    Keywords antigenic imprinting ; SARS‐CoV‐2 ; vaccination strategies ; variants of concern ; Medicine (General) ; R5-920
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Structural mechanisms of GABA

    Noviello, Colleen M / Kreye, Jakob / Teng, Jinfeng / Prüss, Harald / Hibbs, Ryan E

    Cell

    2022  Volume 185, Issue 14, Page(s) 2469–2477.e13

    Abstract: Autoantibodies targeting neuronal membrane proteins can cause encephalitis, seizures, and severe behavioral abnormalities. While antibodies for several neuronal targets have been identified, structural details on how they regulate function are unknown. ... ...

    Abstract Autoantibodies targeting neuronal membrane proteins can cause encephalitis, seizures, and severe behavioral abnormalities. While antibodies for several neuronal targets have been identified, structural details on how they regulate function are unknown. Here we determined cryo-electron microscopy structures of antibodies derived from an encephalitis patient bound to the γ-aminobutyric acid type A (GABA
    MeSH term(s) Autoantibodies ; Cryoelectron Microscopy ; Encephalitis ; Hashimoto Disease ; Humans ; Receptors, GABA-A/metabolism ; gamma-Aminobutyric Acid
    Chemical Substances Autoantibodies ; Receptors, GABA-A ; gamma-Aminobutyric Acid (56-12-2)
    Language English
    Publishing date 2022-07-07
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2022.06.025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1167: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 58: Show issues

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  5. Article ; Online: Do cross-reactive antibodies cause neuropathology in COVID-19?

    Kreye, Jakob / Reincke, S Momsen / Prüss, Harald

    Nature reviews. Immunology

    2020  Volume 20, Issue 11, Page(s) 645–646

    MeSH term(s) Antibodies, Monoclonal/biosynthesis ; Antibodies, Neutralizing/biosynthesis ; Antibodies, Viral/biosynthesis ; Autoimmunity ; Betacoronavirus/immunology ; Betacoronavirus/pathogenicity ; Brain Diseases/complications ; Brain Diseases/diagnosis ; Brain Diseases/immunology ; COVID-19 ; Coronavirus Infections/complications ; Coronavirus Infections/diagnosis ; Coronavirus Infections/immunology ; Cross Reactions ; Headache/complications ; Headache/diagnosis ; Headache/immunology ; Humans ; Pandemics ; Parkinsonian Disorders/complications ; Parkinsonian Disorders/diagnosis ; Parkinsonian Disorders/immunology ; Pneumonia, Viral/complications ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/immunology ; SARS-CoV-2 ; Seizures/complications ; Seizures/diagnosis ; Seizures/immunology ; Tremor/complications ; Tremor/diagnosis ; Tremor/immunology
    Chemical Substances Antibodies, Monoclonal ; Antibodies, Neutralizing ; Antibodies, Viral
    Keywords covid19
    Language English
    Publishing date 2020-10-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 2062776-2
    ISSN 1474-1741 ; 1474-1733
    ISSN (online) 1474-1741
    ISSN 1474-1733
    DOI 10.1038/s41577-020-00458-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5565: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 34: Show issues

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  6. Article ; Online: Brain antibody sequence evaluation (BASE): an easy-to-use software for complete data analysis in single cell immunoglobulin cloning.

    Reincke, S Momsen / Prüss, Harald / Kreye, Jakob

    BMC bioinformatics

    2020  Volume 21, Issue 1, Page(s) 446

    Abstract: Background: Repertoire analysis of patient-derived recombinant monoclonal antibodies is an important tool to study the role of B cells in autoimmune diseases of the human brain and beyond. Current protocols for generation of patient-derived recombinant ... ...

    Abstract Background: Repertoire analysis of patient-derived recombinant monoclonal antibodies is an important tool to study the role of B cells in autoimmune diseases of the human brain and beyond. Current protocols for generation of patient-derived recombinant monoclonal antibody libraries are time-consuming and contain repetitive steps, some of which can be assisted with the help of software automation.
    Results: We developed BASE, an easy-to-use software for complete data analysis in single cell immunoglobulin cloning. BASE consists of two modules: aBASE for immunological annotations and cloning primer lookup, and cBASE for plasmid sequence identity confirmation before expression. Comparing automated BASE analysis with manual analysis we confirmed the validity of BASE output: identity between manual and automated aBASE analysis was 100% for all outputs, except for immunoglobulin isotype determination. In this case, aBASE yielded correct results in 96% of cases, whereas 4% of cases required manual confirmation. cBASE automatically concluded expression recommendations in 89.8% of cases, 91.8% of which were identical to manually derived results and none of them were false-positive.
    Conclusions: BASE offers an easy-to-use software solution suitable for complete Ig sequence data analysis and tracking during recombinant mAb cloning from single cells. Plasmid sequence identity confirmation by cBASE offers functionality not provided by existing software solutions in the field and will help to reduce time-consuming steps of the monoclonal antibody generation workflow. BASE can be installed locally or accessed online at Code Ocean.
    MeSH term(s) Antibodies, Monoclonal/genetics ; Antibodies, Monoclonal/immunology ; Brain/immunology ; Cloning, Molecular ; Data Analysis ; Humans ; Plasmids/genetics ; Sequence Analysis/methods ; Software
    Chemical Substances Antibodies, Monoclonal
    Language English
    Publishing date 2020-10-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041484-5
    ISSN 1471-2105 ; 1471-2105
    ISSN (online) 1471-2105
    ISSN 1471-2105
    DOI 10.1186/s12859-020-03741-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Hodgkin Lymphoma Cell Lines and Tissues Express mGluR5: A Potential Link to Ophelia Syndrome and Paraneoplastic Neurological Disease.

    Schnell, Sofia / Knierim, Ellen / Bittigau, Petra / Kreye, Jakob / Hauptmann, Kathrin / Hundsdoerfer, Patrick / Morales-Gonzalez, Susanne / Schuelke, Markus / Nikolaus, Marc

    Cells

    2023  Volume 12, Issue 4

    Abstract: Ophelia syndrome is characterized by the coincidence of severe neuropsychiatric symptoms, classical Hodgkin lymphoma, and the presence of antibodies to the metabotropic glutamate 5 receptor (mGluR5). Little is known about the pathogenetic link between ... ...

    Abstract Ophelia syndrome is characterized by the coincidence of severe neuropsychiatric symptoms, classical Hodgkin lymphoma, and the presence of antibodies to the metabotropic glutamate 5 receptor (mGluR5). Little is known about the pathogenetic link between these symptoms and the role that anti-mGluR5-antibodies play. We investigated lymphoma tissue from patients with Ophelia syndrome and with isolated classical Hodgkin lymphoma by quantitative immunocytochemistry for mGluR5-expression. Further, we studied the L-1236, L-428, L-540, SUP-HD1, KM-H2, and HDLM-2 classical Hodgkin lymphoma cell lines by FACS and Western blot for mGluR5-expression, and by transcriptome analysis. mGluR5 surface expression differed significantly in terms of receptor density, distribution pattern, and percentage of positive cells. The highest expression levels were found in the L-1236 line. RNA-sequencing revealed more than 800 genes that were higher expressed in the L-1236 line in comparison to the other classical Hodgkin lymphoma cell lines. High mGluR5-expression was associated with upregulation of PI3K/AKT and MAPK pathways and of downstream targets (e.g.,
    MeSH term(s) Humans ; Receptor, Metabotropic Glutamate 5 ; Hodgkin Disease ; Phosphatidylinositol 3-Kinases ; Autoantibodies ; Nervous System Diseases ; Encephalitis ; Syndrome ; Cell Line
    Chemical Substances Receptor, Metabotropic Glutamate 5 ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Autoantibodies
    Language English
    Publishing date 2023-02-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells12040606
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  8. Article ; Online: Converging synaptic and network dysfunctions in distinct autoimmune encephalitis.

    Hunter, Daniel / Petit-Pedrol, Mar / Fernandes, Dominique / Bénac, Nathan / Rodrigues, Catarina / Kreye, Jakob / Ceanga, Mihai / Prüss, Harald / Geis, Christian / Groc, Laurent

    EMBO reports

    2024  Volume 25, Issue 3, Page(s) 1623–1649

    Abstract: Psychiatric and neurological symptoms, as well as cognitive deficits, represent a prominent phenotype associated with variable forms of autoimmune encephalitis, regardless of the neurotransmitter receptor targeted by autoantibodies. The mechanistic ... ...

    Abstract Psychiatric and neurological symptoms, as well as cognitive deficits, represent a prominent phenotype associated with variable forms of autoimmune encephalitis, regardless of the neurotransmitter receptor targeted by autoantibodies. The mechanistic underpinnings of these shared major neuropsychiatric symptoms remain however unclear. Here, we investigate the impacts of patient-derived monoclonal autoantibodies against the glutamatergic NMDAR (NMDAR mAb) and inhibitory GABAaR (GABAaR mAb) signalling in the hippocampal network. Unexpectedly, both excitatory and inhibitory synaptic receptor membrane dynamics, content and transmissions are altered by NMDAR or GABAaR mAb, irrespective of the affinity or antagonistic effect of the autoantibodies. The effect of NMDAR mAb on inhibitory synapses and GABAaR mAb on excitatory synapses requires neuronal activity and involves protein kinase signalling. At the cell level, both autoantibodies increase the excitation/inhibition balance of principal cell inputs. Furthermore, NMDAR or GABAaR mAb leads to hyperactivation of hippocampal networks through distinct alterations of principal cell and interneuron properties. Thus, autoantibodies targeting excitatory NMDAR or inhibitory GABAaR trigger convergent network dysfunctions through a combination of shared and distinct mechanisms.
    MeSH term(s) Humans ; Receptors, GABA-A/metabolism ; Encephalitis ; Autoantibodies/metabolism ; Receptors, N-Methyl-D-Aspartate/metabolism ; Autoimmune Diseases of the Nervous System ; Hashimoto Disease
    Chemical Substances Receptors, GABA-A ; Autoantibodies ; Receptors, N-Methyl-D-Aspartate
    Language English
    Publishing date 2024-01-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2020896-0
    ISSN 1469-3178 ; 1469-221X
    ISSN (online) 1469-3178
    ISSN 1469-221X
    DOI 10.1038/s44319-024-00056-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 5433: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 41: Show issues

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  9. Article ; Online: Brain antibody sequence evaluation (BASE)

    S. Momsen Reincke / Harald Prüss / Jakob Kreye

    BMC Bioinformatics, Vol 21, Iss 1, Pp 1-

    an easy-to-use software for complete data analysis in single cell immunoglobulin cloning

    2020  Volume 10

    Abstract: Abstract Background Repertoire analysis of patient-derived recombinant monoclonal antibodies is an important tool to study the role of B cells in autoimmune diseases of the human brain and beyond. Current protocols for generation of patient-derived ... ...

    Abstract Abstract Background Repertoire analysis of patient-derived recombinant monoclonal antibodies is an important tool to study the role of B cells in autoimmune diseases of the human brain and beyond. Current protocols for generation of patient-derived recombinant monoclonal antibody libraries are time-consuming and contain repetitive steps, some of which can be assisted with the help of software automation. Results We developed BASE, an easy-to-use software for complete data analysis in single cell immunoglobulin cloning. BASE consists of two modules: aBASE for immunological annotations and cloning primer lookup, and cBASE for plasmid sequence identity confirmation before expression. Comparing automated BASE analysis with manual analysis we confirmed the validity of BASE output: identity between manual and automated aBASE analysis was 100% for all outputs, except for immunoglobulin isotype determination. In this case, aBASE yielded correct results in 96% of cases, whereas 4% of cases required manual confirmation. cBASE automatically concluded expression recommendations in 89.8% of cases, 91.8% of which were identical to manually derived results and none of them were false-positive. Conclusions BASE offers an easy-to-use software solution suitable for complete Ig sequence data analysis and tracking during recombinant mAb cloning from single cells. Plasmid sequence identity confirmation by cBASE offers functionality not provided by existing software solutions in the field and will help to reduce time-consuming steps of the monoclonal antibody generation workflow. BASE can be installed locally or accessed online at Code Ocean.
    Keywords Single cell ; B cell ; Antibody ; Immunoglobulin ; Sequence analysis ; Computer applications to medicine. Medical informatics ; R858-859.7 ; Biology (General) ; QH301-705.5
    Subject code 005 ; 004
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Brain blood vessel autoantibodies in patients with NMDA and GABA

    Li, Lucie Y / Kreye, Jakob / Burek, Malgorzata / Cordero-Gomez, César / Barthel, Paula C / Sánchez-Sendín, Elisa / Kornau, Hans-Christian / Schmitz, Dietmar / Scharf, Madeleine / Meybohm, Patrick / Reincke, S Momsen / Prüss, Harald / Höltje, Markus

    Frontiers in cellular neuroscience

    2023  Volume 17, Page(s) 1077204

    Abstract: Introduction: ...

    Abstract Introduction:
    Language English
    Publishing date 2023-01-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2452963-1
    ISSN 1662-5102
    ISSN 1662-5102
    DOI 10.3389/fncel.2023.1077204
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