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Article ; Online: A rapid and sensitive CRISPR-Cas12a for the detection of

Qu, Hai / Zhang, Wenjing / Li, Jianghao / Fu, Qingshan / Li, Xiaoxia / Wang, Miaomiao / Fu, Guangyu / Cui, Jing

2024 Volume 12, Issue 2, Page(s) e0362923

Abstract: Fusobacterium ... ...

Abstract	Fusobacterium nucleatum
MeSH term(s)	Humans ; Fusobacterium nucleatum ; CRISPR-Cas Systems ; Gastrointestinal Tract ; Health Status ; Communicable Diseases ; Recombinases
Chemical Substances	Recombinases
Language	English
Publishing date	2024-01-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.03629-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Study on Corrosion Behavior of Porous Pure Copper Based on Electrochemistry and Scanning Kelvin Probe.

Chen, Xuedan / Liao, Qilong / Zuo, Hanyang / Fu, Qingshan

Materials (Basel, Switzerland)

2023 Volume 16, Issue 23

Abstract: Porous metals are widely used in filtration and separation, flame retardant explosion-proof, biomedical application, etc. Compared with its corresponding dense metal, the presence of porous structures also leads to different corrosive performances in ... ...

Abstract	Porous metals are widely used in filtration and separation, flame retardant explosion-proof, biomedical application, etc. Compared with its corresponding dense metal, the presence of porous structures also leads to different corrosive performances in porous metal. Some studies have utilized the weight loss method, electrochemical impedance to evaluate porous metal corrosion behavior; however, the influence of pore structure on metal corrosion is still ambiguous, and present methods used for analyses of porous metal corrosion are statistical averages of the corrosion behavior of the entire porous material, which cannot accurately reflect the corrosion behavior inside the pores. Herein, we prepare the porous copper samples with 0, 24, 72, and 96 pores using a mechanical process, and employ scanning Kelvin probe combined with electrochemical polarization and impedance spectroscopy to test the corrosion performance of the porous copper in static and dynamic NaCl solutions. The relevant results indicate that in the static solution, the corrosion resistance of the samples gradually increases with the rise in the number of pores. By contrast, in the dynamic solution, the 24-pore sample is more susceptible to corrosion than the sample without the pore.
Language	English
Publishing date	2023-11-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma16237370
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Article ; Online: Arthroscopic treatment of deep gluteal syndrome and the application value of high-frequency ultrasound.

Sun, Guanjun / Fu, Weili / Li, Qingshan / Yin, Yi

BMC musculoskeletal disorders

2023 Volume 24, Issue 1, Page(s) 742

Abstract: Purpose: This study aimed to evaluate the efficacy of arthroscopic sciatic neurolysis for treating deep gluteal syndrome (DGS) and to analyse the application value of high-frequency ultrasound during perioperative period.: Methods: Between June 2020 ... ...

Abstract	Purpose: This study aimed to evaluate the efficacy of arthroscopic sciatic neurolysis for treating deep gluteal syndrome (DGS) and to analyse the application value of high-frequency ultrasound during perioperative period. Methods: Between June 2020 and February 2022, 30 patients with DGS who underwent failed conservative treatment were retrospectively analysed. Lateral arthroscopic exploration of the deep gluteal space and sciatic neurolysis were performed. In addition to pelvic X-ray, lumbar disc and hip magnetic resonance imaging (MRI), ultrasonography of the sciatic nerve was also performed in all patients. The visual analogue scale pain score (VAS), modified Harris hip score (mHHS) and Benson symptom-rating scale were used to evaluate the clinical efficacy. The correlation between preoperative sciatic nerve ultrasound and arthroscopic findings was analysed. Results: The median follow-up for these patients was 13 months (range,12-21 months). Preoperative ultrasonography showed precise morphological changes in 26 sciatic nerves of patients. The VAS score decreased from 5.0 (4.0, 6.0) preoperatively to 0.5 (0, 1.0) postoperatively (p < 0.001), and the mHHS increased from 64.0 (57.0, 67.0) preoperatively to 95.0 (93.0, 97.0) postoperatively (p < 0.001). The Benson symptom score was excellent in 15 cases, good in 12 cases, fair in 2 cases, poor in 1 case; thus, the score was excellent or good in 90% of the cases. Preoperative ultrasound diagnosis and intra-operative findings matched up in all cases. There were four cases of transient numbness in the posterior thigh. Conclusions: Arthroscopic sciatic neurolysis is a safe and effective treatment option for DGS patients who fail conservative treatment. Ultrasound diagnosis matched the arthroscopic findings perfectly. Preoperative Doppler ultrasound can assist surgical decision-making, guide intraoperative release.
MeSH term(s)	Humans ; Retrospective Studies ; Conservative Treatment ; Ultrasonography ; Angiography
Language	English
Publishing date	2023-09-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2041355-5
ISSN	1471-2474 ; 1471-2474
ISSN (online)	1471-2474
ISSN	1471-2474
DOI	10.1186/s12891-023-06863-3
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Article: A trimeric hydrophobic zipper mediates the intramembrane assembly of SARS-CoV-2 spike.

Fu, Qingshan / Chou, James J

bioRxiv : the preprint server for biology

2021

Abstract: The S protein of the SARS-CoV-2 is a Type I membrane protein that mediates membrane fusion and viral entry. A vast amount of structural information is available for the ectodomain of S, a primary target by the host immune system, but much less is known ... ...

Abstract	The S protein of the SARS-CoV-2 is a Type I membrane protein that mediates membrane fusion and viral entry. A vast amount of structural information is available for the ectodomain of S, a primary target by the host immune system, but much less is known regarding its transmembrane domain (TMD) and its membrane-proximal regions. Here, we determined the nuclear magnetic resonance (NMR) structure of the S protein TMD in bicelles that closely mimic a lipid bilayer. The TMD structure is a transmembrane α-helix (TMH) trimer that assembles spontaneously in membrane. The trimer structure shows an extensive hydrophobic core along the 3-fold axis that resembles that of a trimeric leucine/isoleucine zipper, but with tetrad, not heptad, repeat. The trimeric core is strong in bicelles, resisting hydrogen-deuterium exchange for weeks. Although highly stable, structural guided mutagenesis identified single mutations that can completely dissociate the TMD trimer. Multiple studies have shown that the membrane anchor of viral fusion protein can form highly specific oligomers, but the exact function of these oligomers remain unclear. Our findings should guide future experiments to address the above question for SARS coronaviruses.
Language	English
Publishing date	2021-04-10
Publishing country	United States
Document type	Preprint
DOI	10.1101/2021.04.09.439203
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Article ; Online: A Trimeric Hydrophobic Zipper Mediates the Intramembrane Assembly of SARS-CoV-2 Spike.

Fu, Qingshan / Chou, James J

Journal of the American Chemical Society

2021 Volume 143, Issue 23, Page(s) 8543–8546

Abstract: The S protein of SARS-CoV-2 is a type I membrane protein that mediates membrane fusion and viral entry. A vast amount of structural information is available for the ectodomain of S, a primary target by the host immune system, but much less is known ... ...

Abstract	The S protein of SARS-CoV-2 is a type I membrane protein that mediates membrane fusion and viral entry. A vast amount of structural information is available for the ectodomain of S, a primary target by the host immune system, but much less is known regarding its transmembrane domain (TMD) and its membrane-proximal regions. Here, we determined the NMR structure of the S protein TMD in bicelles that closely mimic a lipid bilayer. The TMD structure is a transmembrane α-helix (TMH) trimer that assembles spontaneously in a membrane. The trimer structure shows an extensive hydrophobic core along the 3-fold axis that resembles that of a trimeric leucine/isoleucine zipper, but with tetrad, not heptad, repeats. The trimeric core is strong in bicelles, resisting hydrogen-deuterium exchange for weeks. Although highly stable, structural guided mutagenesis identified single mutations that can completely dissociate the TMD trimer. Multiple studies have shown that the membrane anchors of viral fusion proteins can form highly specific oligomers, but the exact function of these oligomers remains unclear. Our findings should guide future experiments to address the above question for SARS coronaviruses.
MeSH term(s)	Cell Membrane/metabolism ; Hydrophobic and Hydrophilic Interactions ; Models, Molecular ; Protein Multimerization ; Protein Structure, Quaternary ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/metabolism
Chemical Substances	Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2021-06-04
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.1c02394
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Article: A Trimeric Hydrophobic Zipper Mediates the Intramembrane Assembly of SARS-CoV-2 Spike

Fu, Qingshan / Chou, James J.

Journal of the American Chemical Society. 2021 June 04, v. 143, no. 23

2021

Abstract	The S protein of SARS-CoV-2 is a type I membrane protein that mediates membrane fusion and viral entry. A vast amount of structural information is available for the ectodomain of S, a primary target by the host immune system, but much less is known regarding its transmembrane domain (TMD) and its membrane-proximal regions. Here, we determined the NMR structure of the S protein TMD in bicelles that closely mimic a lipid bilayer. The TMD structure is a transmembrane α-helix (TMH) trimer that assembles spontaneously in a membrane. The trimer structure shows an extensive hydrophobic core along the 3-fold axis that resembles that of a trimeric leucine/isoleucine zipper, but with tetrad, not heptad, repeats. The trimeric core is strong in bicelles, resisting hydrogen–deuterium exchange for weeks. Although highly stable, structural guided mutagenesis identified single mutations that can completely dissociate the TMD trimer. Multiple studies have shown that the membrane anchors of viral fusion proteins can form highly specific oligomers, but the exact function of these oligomers remains unclear. Our findings should guide future experiments to address the above question for SARS coronaviruses.
Keywords	Severe acute respiratory syndrome coronavirus 2 ; Severe acute respiratory syndrome-related coronavirus ; hydrophobicity ; immune system ; isoleucine ; leucine ; lipid bilayers ; membrane fusion ; membrane proteins ; mutagenesis
Language	English
Dates of publication	2021-0604
Size	p. 8543-8546.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.1c02394
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Insights into the Overcoming EGFR

Zhang, Xuan / He, Jie / Xu, Shidi / Fu, Li / Zheng, Pengwu / Xu, Shan / Pan, Qingshan / Zhu, Wufu

ChemMedChem

2024 , Page(s) e202300634

Abstract: The epithelial growth factor receptor (EGFR) signaling pathway has been proposed to benefit non-small cell lung cancer (NSCLC) treatment. In this manuscript, we investigated the modification of 2-aryl-4-aminoquinazoline, the classical backbone of the ... ...

Abstract	The epithelial growth factor receptor (EGFR) signaling pathway has been proposed to benefit non-small cell lung cancer (NSCLC) treatment. In this manuscript, we investigated the modification of 2-aryl-4-aminoquinazoline, the classical backbone of the fourth-generation EGFR inhibitors, in addition to obtaining a series of novel 2-aryl-4-aminothienopyrimidine derivatives (A1~A45), we also gained further understanding of the modification of this framework. Derivatives were tested for cytotoxicity against cancer cell lines (cervical cancer cell line Hela, lung cancer cell lines A549, H1975, and PC-9, Ba/F3-EGFR
Language	English
Publishing date	2024-02-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2218496-X
ISSN	1860-7187 ; 1860-7179
ISSN (online)	1860-7187
ISSN	1860-7179
DOI	10.1002/cmdc.202300634
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Article ; Online: Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition.

Chen, Jiali / Wang, Yifan / Lin, Shuru / Yu, Quanxiang / Qi, Zhengfei / Jiang, Wenqi / Zhao, Qiang / Fu, Qingshan Bill

Biochemical and biophysical research communications

2023 Volume 694, Page(s) 149395

Abstract: SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM ...

Abstract	SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM and its mutant SpoVM
MeSH term(s)	Green Fluorescent Proteins/metabolism ; Adenosine Triphosphate/metabolism ; Bacterial Proteins/metabolism ; Bacillus subtilis/metabolism ; Spores, Bacterial/metabolism
Chemical Substances	Green Fluorescent Proteins (147336-22-9) ; Adenosine Triphosphate (8L70Q75FXE) ; Bacterial Proteins
Language	English
Publishing date	2023-12-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2023.149395
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Article ; Online: Fabrication of versatile polyvinyl alcohol and carboxymethyl cellulose-based hydrogels for information hiding and flexible sensors: Heat-induced adjustable stiffness and transparency.

Sun, Yuanna / Shi, Fenling / Tian, Ruobing / Zhao, Xiaoliang / Li, Qingshan / Song, Chen / Du, Ying / He, Xinhai / Fu, Jun

International journal of biological macromolecules

2023 Volume 253, Issue Pt 3, Page(s) 126950

Abstract: With the growing demand for wearable electronics, designing biocompatible hydrogels that combine self-repairability, wide operating temperature and precise sensing ability offers a promising scheme. Herein, by interpenetrating naturally derived ... ...

Abstract	With the growing demand for wearable electronics, designing biocompatible hydrogels that combine self-repairability, wide operating temperature and precise sensing ability offers a promising scheme. Herein, by interpenetrating naturally derived carboxymethyl cellulose (CMC) into a polyvinyl alcohol (PVA) gel matrix, a novel hydrogel is successfully developed via simple coordination with calcium chloride (CaCl
MeSH term(s)	Humans ; Hot Temperature ; Carboxymethylcellulose Sodium ; Polyvinyl Alcohol ; Temperature ; Electric Conductivity ; Hydrogels
Chemical Substances	Carboxymethylcellulose Sodium (K679OBS311) ; Polyvinyl Alcohol (9002-89-5) ; Hydrogels
Language	English
Publishing date	2023-09-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.126950
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Article ; Online: A trimeric hydrophobic zipper mediates the intramembrane assembly of SARS-CoV-2 spike

Fu, Qingshan / Chou, James J

bioRxiv

Abstract	The S protein of the SARS-CoV-2 is a Type I membrane protein that mediates membrane fusion and viral entry. A vast amount of structural information is available for the ectodomain of S, a primary target by the host immune system, but much less is known regarding its transmembrane domain (TMD) and its membrane-proximal regions. Here, we determined the nuclear magnetic resonance (NMR) structure of the S protein TMD in bicelles that closely mimic a lipid bilayer. The TMD structure is a transmembrane α-helix (TMH) trimer that assembles spontaneously in membrane. The trimer structure shows an extensive hydrophobic core along the 3-fold axis that resembles that of a trimeric leucine/isoleucine zipper, but with tetrad, not heptad, repeat. The trimeric core is strong in bicelles, resisting hydrogen-deuterium exchange for weeks. Although highly stable, structural guided mutagenesis identified single mutations that can completely dissociate the TMD trimer. Multiple studies have shown that the membrane anchor of viral fusion protein can form highly specific oligomers, but the exact function of these oligomers remain unclear. Our findings should guide future experiments to address the above question for SARS coronaviruses.
Keywords	covid19
Language	English
Publishing date	2021-04-10
Publisher	Cold Spring Harbor Laboratory
Document type	Article ; Online
DOI	10.1101/2021.04.09.439203
Database	COVID19

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