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Article ; Online: Massarilactones D and H, phytotoxins produced by

Cimmino, Alessio / Bahmani, Zeinab / Masi, Marco / Di Lecce, Roberta / Amini, Jahanshir / Abdollahzadeh, Jafar / Tuzi, Angela / Evidente, Antonio

Natural product research

2020 Volume 35, Issue 23, Page(s) 5192–5198

Abstract: ... A strain ... ...

Abstract	A strain of
MeSH term(s)	Ascomycota ; Iran ; Plant Diseases ; Vitis
Language	English
Publishing date	2020-08-03
Publishing country	England
Document type	Journal Article
ZDB-ID	2185747-7
ISSN	1478-6427 ; 1478-6419
ISSN (online)	1478-6427
ISSN	1478-6419
DOI	10.1080/14786419.2020.1791116
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Characteristics of 26 kDa antigen of H. Pylori by Monoclonal Antibody.

Ghahremani, Hossein / Farshad, Shohreh / Amini Najafabadi, Hossein / Kashanian, Susan / Momeni Moghaddam, Mohammad Amin / Moradi, Nariman / Paknejad, Maliheh

Iranian journal of allergy, asthma, and immunology

2015 Volume 14, Issue 1, Page(s) 113–119

Abstract: ... in Helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect H. pylori ... against 26 kDa antigen of H. pylori were cultivated and MAbs were purified by affinity chromatography ... sandwich ELISA. Our findings showed that the MAbs against 26 kDa antigen of H. pylori could recognize three ...

Abstract	Alkylhydroperoxide reductase (AhpC, the 26 kDa antigen) is one of the abundant antioxidant enzymes in Helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect H. pylori infection in clinical specimens. This study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. Five established hybridoma cell lines secreting monoclonal antibodies (MAbs) against 26 kDa antigen of H. pylori were cultivated and MAbs were purified by affinity chromatography. Subsequently, MAbs were conjugated with biotin, and different combinations of capture and tracer antibodies used in sandwich ELISA. Immunoblotting of bacterial extracts were performed to estimate aggregation status of the antigen. Release of antigen from the cultivated bacteria on solid media was examined by sandwich ELISA, and also, existence of interference in fecal extract was investigated by immunoblotting and sandwich ELISA. Our findings showed that the MAbs against 26 kDa antigen of H. pylori could recognize three bands of nearly 25 kDa, 50 kDa, and 75 kDa in immunoblotting. This study also indicated presence of more antigens in the culture medium around the bacteria than the bacterial extract itself. The results of sandwich ELISA and immunoblotting on fecal extracts suggest the presence of interfering agents that prevent detection of antigen by antibody in ELISA but not in immunoblotting. In this study the oligomerization of the 26 kDa antigen, presence of interfering agents in stool matrix, and release of antigen to outside of bacteria, were demonstrated.
MeSH term(s)	Antibodies, Monoclonal ; Bacterial Proteins/isolation & purification ; Enzyme-Linked Immunosorbent Assay ; Feces/microbiology ; Helicobacter Infections/diagnosis ; Humans ; Peroxidases/isolation & purification
Chemical Substances	Antibodies, Monoclonal ; Bacterial Proteins ; Peroxidases (EC 1.11.1.-) ; ahpC protein, Helicobacter pylori (EC 1.11.1.15)
Language	English
Publishing date	2015-02
Publishing country	Iran
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2488724-9
ISSN	1735-1502
ISSN	1735-1502
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Article: Elucidating Drought-Tolerance Mechanisms in Plant Roots through ¹H NMR Metabolomics in Parallel with MALDI-MS, and NanoSIMS Imaging Techniques

Environmental science & technology. 2022 Jan. 20, v. 56, no. 3

2022

Abstract: ... enhanced root structural defense via recalcitrant compounds including lignin, while H. rosa sinensis ...

Abstract	As direct mediators between plants and soil, roots play an important role in metabolic responses to environmental stresses such as drought, yet these responses are vastly uncharacterized on a plant-specific level, especially for co-occurring species. Here, we aim to examine the effects of drought on root metabolic profiles and carbon allocation pathways of three tropical rainforest species by combining cutting-edge metabolomic and imaging technologies in an in situ position-specific ¹³C-pyruvate root-labeling experiment. Further, washed (rhizosphere-depleted) and unwashed roots were examined to test the impact of microbial presence on root metabolic pathways. Drought had a species-specific impact on the metabolic profiles and spatial distribution in Piper sp. and Hibiscus rosa sinensis roots, signifying different defense mechanisms; Piper sp. enhanced root structural defense via recalcitrant compounds including lignin, while H. rosa sinensis enhanced biochemical defense via secretion of antioxidants and fatty acids. In contrast, Clitoria fairchildiana, a legume tree, was not influenced as much by drought but rather by rhizosphere presence where carbohydrate storage was enhanced, indicating a close association with symbiotic microbes. This study demonstrates how multiple techniques can be combined to identify how plants cope with drought through different drought-tolerance strategies and the consequences of such changes on below-ground organic matter composition.
Keywords	Clitoria fairchildiana ; Hibiscus rosa-sinensis ; Piper ; carbohydrates ; carbon ; drought ; drought tolerance ; environmental science ; legumes ; lignin ; metabolomics ; organic matter ; rhizosphere ; secretion ; soil ; trees ; tropical rain forests
Language	English
Dates of publication	2022-0120
Size	p. 2021-2032.
Publishing place	American Chemical Society
Document type	Article
ISSN	1520-5851
DOI	10.1021/acs.est.1c06772
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Massarilactones D and H, phytotoxins produced by Kalmusia variispora, associated with grapevine trunk diseases (GTDs) in Iran

Cimmino, Alessio / Bahmani, Zeinab / Masi, Marco / Di Lecce, Roberta / Amini, Jahanshir / Abdollahzadeh, Jafar / Tuzi, Angela / Evidente, Antonio

Natural Product Research. 2021 Dec. 2, v. 35, no. 23 p.5192-5198

2021

Abstract: ... and ¹³C NMR and HR ESIMS) methods as the fungal polyketides massarilactones D and H (1 and 2 ... analysis. Massarilactones D and H showed phytotoxic activity on Vitis vinifera L. at two concentrations ... filtrates. They were identified by physic (specific optical rotation), and spectroscopic (essentially 1D ¹H ...

Abstract	A strain of Kalmusia variispora associated with grapevine trunk diseases (GTDs) was identified in Iran and induced disease symptoms on the host in greenhouse conditions. The grapevine pathogens are able to produce a plethora of toxic metabolites belonging to different classes of naturally occurring compounds. Two homogeneous compounds were isolated from the organic extract of K. variispora culture filtrates. They were identified by physic (specific optical rotation), and spectroscopic (essentially 1D ¹H and ¹³C NMR and HR ESIMS) methods as the fungal polyketides massarilactones D and H (1 and 2). The unassigned absolute configuration of massarilactone D was unambiguously determined by X-ray diffractometric analysis. Massarilactones D and H showed phytotoxic activity on Vitis vinifera L. at two concentrations used and depending from the days of inoculation. Phytotoxicity is also increased when the 3,4,7-O,O',O"-triacetyl derivative of massarilactone D (3) was assayed on the host plant. This is the first report on the investigation of phytotoxic metabolites produced by K. variispora isolated from infected grapevine in Iran and they seem to be involved in the development of disease symptoms.
Keywords	Vitis vinifera ; X-ray diffraction ; fungi ; greenhouses ; host plants ; metabolites ; phytotoxicity ; phytotoxins ; polyketides ; research ; spectroscopy ; Iran ; Grapevine trunk diseases (GTDs) ; Kalmusia variispora ; massarilactones D and H ; absolute configuration of massarilactone D
Language	English
Dates of publication	2021-1202
Size	p. 5192-5198.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	2185747-7
ISSN	1478-6427 ; 1478-6419
ISSN (online)	1478-6427
ISSN	1478-6419
DOI	10.1080/14786419.2020.1791116
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effect of the Ratio of Magnetite Particle Size to Microwave Penetration Depth on Reduction Reaction Behaviour by H

Amini, Ahmadreza / Ohno, Ko-Ichiro / Maeda, Takayuki / Kunitomo, Kazuya

Scientific reports

2018 Volume 8, Issue 1, Page(s) 15023

Abstract: In this study, we investigated reduction of magnetite by H ...

Abstract	In this study, we investigated reduction of magnetite by H
Language	English
Publishing date	2018-10-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-018-33460-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The role of the VZn-NO-H complex in the p-type conductivity in ZnO.

Amini, M N / Saniz, R / Lamoen, D / Partoens, B

Physical chemistry chemical physics : PCCP

2015 Volume 17, Issue 7, Page(s) 5485–5489

Abstract: ... The p-type conductivity was attributed to a VZn-NO-H shallow acceptor complex, formed by a Zn vacancy ... VZn), N substituting O (NO), and H interstitial (Hi). We present here a first-principles hybrid ... functional study of this complex compared to the one without hydrogen. Our results confirm that the VZn-NO-H ...

Abstract	Past research efforts aiming at obtaining stable p-type ZnO have been based on complexes involving nitrogen doping. A recent experiment by (J. G. Reynolds et al., Appl. Phys. Lett., 2013, 102, 152114) demonstrated a significant (∼10(18) cm(-3)) p-type behavior in N-doped ZnO films after appropriate annealing. The p-type conductivity was attributed to a VZn-NO-H shallow acceptor complex, formed by a Zn vacancy (VZn), N substituting O (NO), and H interstitial (Hi). We present here a first-principles hybrid functional study of this complex compared to the one without hydrogen. Our results confirm that the VZn-NO-H complex acts as an acceptor in ZnO. We find that H plays an important role, because it lowers the formation energy of the complex with respect to VZn-NO, a complex known to exhibit (unstable) p-type behavior. However, this additional H atom also occupies the hole level at the origin of the shallow behavior of VZn-NO, leaving only two states empty higher in the band gap and making the VZn-NO-H complex a deep acceptor. Therefore, we conclude that the cause of the observed p-type conductivity in experiment is not the presence of the VZn-NO-H complex, but probably the formation of the VZn-NO complex during the annealing process.
Language	English
Publishing date	2015-02-21
Publishing country	England
Document type	Journal Article
ZDB-ID	1476244-4
ISSN	1463-9084 ; 1463-9076
ISSN (online)	1463-9084
ISSN	1463-9076
DOI	10.1039/c4cp05894a
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Article ; Online: The Potential of Gas Switching Partial Oxidation Using Advanced Oxygen Carriers for Efficient H 2 Production with Inherent CO 2 Capture

Carlos Arnaiz del Pozo / Schalk Cloete / Ángel Jiménez Álvaro / Felix Donat / Shahriar Amini

Applied Sciences, Vol 11, Iss 4713, p

2021 Volume 4713

Abstract: ... with the upper bound only achievable by large power and H 2 co-production plants employing a highly efficient ...

Abstract	The hydrogen economy has received resurging interest in recent years, as more countries commit to net-zero CO 2 emissions around the mid-century. “Blue” hydrogen from natural gas with CO 2 capture and storage (CCS) is one promising sustainable hydrogen supply option. Although conventional CO 2 capture imposes a large energy penalty, advanced process concepts using the chemical looping principle can produce blue hydrogen at efficiencies even exceeding the conventional steam methane reforming (SMR) process without CCS. One such configuration is gas switching reforming (GSR), which uses a Ni-based oxygen carrier material to catalyze the SMR reaction and efficiently supply the required process heat by combusting an off-gas fuel with integrated CO 2 capture. The present study investigates the potential of advanced La-Fe-based oxygen carrier materials to further increase this advantage using a gas switching partial oxidation (GSPOX) process. These materials can overcome the equilibrium limitations facing conventional catalytic SMR and achieve direct hydrogen production using a water-splitting reaction. Results showed that the GSPOX process can achieve mild efficiency improvements relative to GSR in the range of 0.6–4.1%-points, with the upper bound only achievable by large power and H 2 co-production plants employing a highly efficient power cycle. These performance gains and the avoidance of toxicity challenges posed by Ni-based oxygen carriers create a solid case for the further development of these advanced materials. If successful, results from this work indicate that GSPOX blue hydrogen plants can outperform an SMR benchmark with conventional CO 2 capture by more than 10%-points, both in terms of efficiency and CO 2 avoidance.
Keywords	H 2 -power cogeneration ; gas switching partial oxidation ; gas switching reforming ; CO 2 capture and storage ; steam methane reforming ; blue hydrogen ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Characteristics of 26 kDa antigen of H. Pylori by Monoclonal Antibody.

Hossein Ghahremani / Shohreh Farshad / Hossein Amini Najafabadi / Susan Kashanian / Mohammad Amin Momeni Moghaddam / Nariman Moradi / Maliheh Paknejad

Iranian Journal Of Allergy, Asthma and Immunology, Vol 14, Iss 1, Pp 113-

2015 Volume 9

Abstract	Alkylhydroperoxide reductase (AhpC, the 26 kDa antigen) is one of the abundant antioxidant enzymes in Helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect H. pylori infection in clinical specimens. This study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. Five established hybridoma cell lines secreting monoclonal antibodies (MAbs) against 26 kDa antigen of H. pylori were cultivated and MAbs were purified by affinity chromatography. Subsequently, MAbs were conjugated with biotin, and different combinations of capture and tracer antibodies used in sandwich ELISA. Immunoblotting of bacterial extracts were performed to estimate aggregation status of the antigen. Release of antigen from the cultivated bacteria on solid media was examined by sandwich ELISA, and also, existence of interference in fecal extract was investigated by immunoblotting and sandwich ELISA. Our findings showed that the MAbs against 26 kDa antigen of H. pylori could recognize three bands of nearly 25 kDa, 50 kDa, and 75 kDa in immunoblotting. This study also indicated presence of more antigens in the culture medium around the bacteria than the bacterial extract itself. The results of sandwich ELISA and immunoblotting on fecal extracts suggest the presence of interfering agents that prevent detection of antigen by antibody in ELISA but not in immunoblotting. In this study the oligomerization of the 26 kDa antigen, presence of interfering agents in stool matrix, and release of antigen to outside of bacteria, were demonstrated.
Keywords	Immunologic diseases. Allergy ; RC581-607 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245 ; Medicine ; R
Subject code	630
Language	English
Publishing date	2015-02-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Characteristics of 26 kDa antigen of H. Pylori by Monoclonal Antibody.

Hossein Ghahremani / Shohreh Farshad / Hossein Amini Najafabadi / Susan Kashanian / Mohammad Amin Momeni Moghaddam / Nariman Moradi / Maliheh Paknejad

Iranian Journal of Allergy, Asthma and Immunology, Vol 14, Iss 1, Pp 113-

2015 Volume 9

Abstract	Alkylhydroperoxide reductase (AhpC, the 26 kDa antigen) is one of the abundant antioxidant enzymes in Helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect H. pylori infection in clinical specimens. This study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. Five established hybridoma cell lines secreting monoclonal antibodies (MAbs) against 26 kDa antigen of H. pylori were cultivated and MAbs were purified by affinity chromatography. Subsequently, MAbs were conjugated with biotin, and different combinations of capture and tracer antibodies used in sandwich ELISA. Immunoblotting of bacterial extracts were performed to estimate aggregation status of the antigen. Release of antigen from the cultivated bacteria on solid media was examined by sandwich ELISA, and also, existence of interference in fecal extract was investigated by immunoblotting and sandwich ELISA. Our findings showed that the MAbs against 26 kDa antigen of H. pylori could recognize three bands of nearly 25 kDa, 50 kDa, and 75 kDa in immunoblotting. This study also indicated presence of more antigens in the culture medium around the bacteria than the bacterial extract itself. The results of sandwich ELISA and immunoblotting on fecal extracts suggest the presence of interfering agents that prevent detection of antigen by antibody in ELISA but not in immunoblotting. In this study the oligomerization of the 26 kDa antigen, presence of interfering agents in stool matrix, and release of antigen to outside of bacteria, were demonstrated.
Keywords	Medicine ; R
Subject code	630
Language	English
Publishing date	2015-02-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Why 24-h Urine Albumin Excretion Rate Method Still is Used for Screening of Diabetic Nephropathy in Isfahan Laboratories?

Teimoury, Azam / Iraj, Bijan / Heidari-Beni, Motahar / Amini, Massoud / Hosseiny, Seyed-Mohsen

International journal of preventive medicine

2014 Volume 5, Issue 3, Page(s) 341–347

Abstract: ... in Iranian diabetic patients.: Methods: A total of 200 diabetic patients participated to our study. 24 h ... timed urine specimens followed by RUS were collected. 24-h urine albumin excretion (24-h urinary albumin ... correlation of coefficient for 24-h UAE versus UACR was 0.64. The area under ROC curve for UACR was 0.83 ...

Abstract	Background: The first step in diagnosis of diabetic nephropathy is measurement of albumin in a spot urine sample. The aim of this study was assessment of the accuracy of urinary albumin to creatinine ratio (UACR) in random urine specimens (RUS) for microalbuminuria and macroalbuminuria screening in Iranian diabetic patients. Methods: A total of 200 diabetic patients participated to our study. 24 h timed urine specimens followed by RUS were collected. 24-h urine albumin excretion (24-h urinary albumin excretion (UAE)) and UACR in RUS were measured. Data were analyzed by Pearson's correlation, receiver operating characteristic (ROC) curve and McNemar test. Results: A total of 165 patients finalized the study. Pearson's correlation of coefficient for 24-h UAE versus UACR was 0.64. The area under ROC curve for UACR was 0.83 in microalbuminuria and 0.91 in macroalbuminuria. The cutoff point of 30 mg/g in UACR method had 86% sensitivity and 60% specificity for microalbuminuria screening and cut-off point of 300 mg/g had 75% sensitivity and 99% specificity for macroalbuminuria screening respectively. Conclusions: UACR in RUS showed acceptable performance as a screening test for diagnosis of both micro and macroalbuminuria in Iranian diabetic patients.
Language	English
Publishing date	2014-04-16
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2574680-7
ISSN	2008-8213 ; 2008-7802
ISSN (online)	2008-8213
ISSN	2008-7802
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