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Article: A Wash of Ethyl Acetoacetate Reduces Externally Added

Horne, Shelley M / Prüß, Birgit M

2022 Volume 11, Issue 8

Abstract: The continuously high numbers of food-borne disease outbreaks document that current intervention techniques are not yet satisfactory. This study describes a novel wash for tomatoes that can be used as part of the food processing chain and is designed to ... ...

Abstract	The continuously high numbers of food-borne disease outbreaks document that current intervention techniques are not yet satisfactory. This study describes a novel wash for tomatoes that can be used as part of the food processing chain and is designed to prevent contamination with serovars of
Language	English
Publishing date	2022-08-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2681345-2
ISSN	2079-6382
ISSN	2079-6382
DOI	10.3390/antibiotics11081134
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Article ; Online: Involvement of Two-Component Signaling on Bacterial Motility and Biofilm Development.

Prüß, Birgit M

Journal of bacteriology

2017 Volume 199, Issue 18

Abstract: Two-component signaling is a specialized mechanism that bacteria use to respond to changes in their environment. Nonpathogenic strains ... ...

Abstract	Two-component signaling is a specialized mechanism that bacteria use to respond to changes in their environment. Nonpathogenic strains of
MeSH term(s)	Biofilms/growth & development ; Escherichia coli K12/physiology ; Gene Expression Regulation, Bacterial ; Locomotion ; Signal Transduction
Language	English
Publishing date	2017-09-15
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2968-3
ISSN	1098-5530 ; 0021-9193
ISSN (online)	1098-5530
ISSN	0021-9193
DOI	10.1128/JB.00259-17
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Article ; Online: Plant seedlings of peas, tomatoes, and cucumbers exude compounds that are needed for growth and chemoattraction of

Nisha, Fatema A / Tagoe, Janice N A / Pease, Amanda B / Horne, Shelley M / Ugrinov, Angel / Geddes, Barney A / Prüß, Birgit M

Canadian journal of microbiology

2024 Volume 70, Issue 5, Page(s) 150–162

Abstract: This study characterizes seedling exudates of peas, tomatoes, and cucumbers at the level of chemical composition and functionality. A plant experiment confirmed ... ...

Abstract	This study characterizes seedling exudates of peas, tomatoes, and cucumbers at the level of chemical composition and functionality. A plant experiment confirmed that
MeSH term(s)	Solanum lycopersicum/microbiology ; Solanum lycopersicum/growth & development ; Cucumis sativus/microbiology ; Cucumis sativus/growth & development ; Seedlings/growth & development ; Seedlings/microbiology ; Rhizobium leguminosarum/growth & development ; Rhizobium leguminosarum/metabolism ; Azospirillum brasilense/growth & development ; Azospirillum brasilense/metabolism ; Pisum sativum/microbiology ; Pisum sativum/growth & development ; Plant Roots/microbiology ; Plant Roots/growth & development ; Chemotaxis ; Plant Exudates/chemistry ; Plant Exudates/metabolism
Chemical Substances	Plant Exudates
Language	English
Publishing date	2024-03-01
Publishing country	Canada
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	280534-0
ISSN	1480-3275 ; 0008-4166
ISSN (online)	1480-3275
ISSN	0008-4166
DOI	10.1139/cjm-2023-0217
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Article ; Online: Migration Rates on Swim Plates Vary between Escherichia coli Soil Isolates: Differences Are Associated with Variants in Metabolic Genes.

Prüß, Birgit M / Horne, Shelley M / Bauer, Erika Shay / Pirner, Collin / Schwartz, Madelyn / Petersen, Morgan L / Bergholz, Peter W

Applied and environmental microbiology

2023 Volume 89, Issue 2, Page(s) e0172722

Abstract: This study investigates migration phenotypes of 265 Escherichia coli soil isolates from the Buffalo River basin in Minnesota, USA. Migration rates on semisolid tryptone swim plates ranged from nonmotile to 190% of the migration rate of a highly motile E. ...

Abstract	This study investigates migration phenotypes of 265 Escherichia coli soil isolates from the Buffalo River basin in Minnesota, USA. Migration rates on semisolid tryptone swim plates ranged from nonmotile to 190% of the migration rate of a highly motile E. coli K-12 strain. The nonmotile isolate, LGE0550, had mutations in flagellar and chemotaxis genes, including two IS
MeSH term(s)	Animals ; Humans ; Escherichia coli ; Soil ; Base Composition ; Genome-Wide Association Study ; Sequence Analysis, DNA ; Phylogeny ; RNA, Ribosomal, 16S ; Escherichia coli Proteins/genetics
Chemical Substances	Soil ; RNA, Ribosomal, 16S ; Escherichia coli Proteins
Language	English
Publishing date	2023-01-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/aem.01727-22
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Article ; Online: Efficacy of β-phenylethylamine as a novel anti-microbial and application as a liquid catheter flush.

Schroeder, Meredith / Horne, Shelley M / Prüß, Birgit M

Journal of medical microbiology

2018 Volume 67, Issue 12, Page(s) 1778–1788

Abstract: With this study, we introduce a liquid flush for catheters and other tubing-based applications that consists of a solution of β-phenylethylamine (PEA) in tryptic soy broth. The initial experiments in multiwell polystyrene plates were conducted with ... ...

Abstract	With this study, we introduce a liquid flush for catheters and other tubing-based applications that consists of a solution of β-phenylethylamine (PEA) in tryptic soy broth. The initial experiments in multiwell polystyrene plates were conducted with Escherichia coli K-12 to assess the effectiveness of PEA at reducing planktonic growth, as well as the biomass and adenosine triphosphate (ATP) content of biofilm; PEA reduced these growth parameters as a function of increasing concentration. This effect was also seen in mutants of PEA catabolism, which leads us to believe that the PEA effect is due to PEA itself and not one of its degradation products. Since PEA reduced planktonic growth and biofilm when added at the time of inoculation, as well as at later time points, we propose PEA as a novel compound for the prevention and treatment of biofilm. PEA reduced planktonic growth and the ATP content of the biofilm for five bacterial pathogens, including an enterohemorrhagic E. coli, two uropathogenic E. coli, Pseudomonas aeruginosa and Staphylococcus aureus. A major finding of this study is the reduction of the ATP content of biofilm that formed in silicone tubing by periodic flushes of PEA. This experiment was performed to model antibiotic-lock treatment of an intravenous catheter. It was found that 10 mg ml
MeSH term(s)	Adenosine Triphosphate/metabolism ; Bacteria/drug effects ; Biofilms/drug effects ; Catheter-Related Infections/microbiology ; Catheter-Related Infections/prevention & control ; Catheters, Indwelling/microbiology ; Escherichia coli K12/drug effects ; Mutation ; Phenethylamines/pharmacology ; Polystyrenes ; Silicones ; Surface Properties
Chemical Substances	Phenethylamines ; Polystyrenes ; Silicones ; phenethylamine (327C7L2BXQ) ; Adenosine Triphosphate (8L70Q75FXE)
Language	English
Publishing date	2018-10-16
Publishing country	England
Document type	Journal Article
ZDB-ID	218356-0
ISSN	1473-5644 ; 0022-2615
ISSN (online)	1473-5644
ISSN	0022-2615
DOI	10.1099/jmm.0.000840
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Zs.A 634: Show issues

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Article: β-phenylethylamine, a small molecule with a large impact.

Irsfeld, Meredith / Spadafore, Matthew / Prüß, Birgit M

WebmedCentral

2014 Volume 4, Issue 9

Abstract: During a screen of bacterial nutrients as inhibitors ... ...

Abstract	During a screen of bacterial nutrients as inhibitors of
Language	English
Publishing date	2014-01-15
Publishing country	England
Document type	Journal Article
ISSN	2046-1690
ISSN	2046-1690
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Article ; Online: Screening the mechanical stability of Escherichia coli biofilms through exposure to external, hydrodynamic shear forces.

Irsfeld, Meredith / Prüß, Birgit M / Stafslien, Shane J

Journal of basic microbiology

2014 Volume 54, Issue 12, Page(s) 1403–1409

Abstract: The desire to attain a deeper understanding of the fundamental aspects governing the mechanical properties of biofilms has become more prominent in recent years. This has largely been due to the realization that these sessile microbial communities often ... ...

Abstract	The desire to attain a deeper understanding of the fundamental aspects governing the mechanical properties of biofilms has become more prominent in recent years. This has largely been due to the realization that these sessile microbial communities often withstand environments where hydrodynamic turbulence and shearing forces are considerable. In the present study, Escherichia coli K-12 was used as a model system to develop a laboratory technique that can be used to quickly screen the mechanical integrity or stability of laboratory cultivated bacterial biofilms when exposed to such external, hydrodynamic shear forces. The screening method utilizes a custom-built, automated water jetting apparatus to generate and precisely apply a pressurized stream of water directly to biofilms cultured in multi-well plates. An optimized set of water jetting parameters was determined to resolve subtle to moderate differences in the mechanical stability of isogenic strains of E. coli K-12 as a function of percent biofilm removal. Mutations in both flagella biosynthesis (fliA) and acetate metabolism (ackA and ackA pta) were shown to impair the mechanical integrity of 24-h biofilms, while a "housekeeping" strain deficient in arginine metabolism (argD) exhibited a mechanical stability profile comparable to the parent strain.
MeSH term(s)	Biofilms ; Escherichia coli K12/physiology ; Fimbriae, Bacterial/metabolism ; Flagella/metabolism ; Hydrodynamics ; Mutation ; Stress, Mechanical
Language	English
Publishing date	2014-12
Publishing country	Germany
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	632513-0
ISSN	1521-4028 ; 0233-111X
ISSN (online)	1521-4028
ISSN	0233-111X
DOI	10.1002/jobm.201400054
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Zs.A 824: Show issues

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Article ; Online: Acetate metabolism and Escherichia coli biofilm: new approaches to an old problem.

Lynnes, Ty / Prüss, Birgit M / Samanta, Priyankar

FEMS microbiology letters

2013 Volume 344, Issue 2, Page(s) 95–103

Abstract: Current antibiotics continue to lose effectiveness for infectious diseases, especially in cases where the bacteria from a biofilm. This review article summarizes control mechanisms for bacterial biofilm, with an emphasis on the modification of signal ... ...

Abstract	Current antibiotics continue to lose effectiveness for infectious diseases, especially in cases where the bacteria from a biofilm. This review article summarizes control mechanisms for bacterial biofilm, with an emphasis on the modification of signal transduction pathways, such as quorum sensing and two-component signaling, by externally added metabolic intermediates. As a link between central metabolism and signal transduction, we discuss the activation of two-component response regulators by activated acetate intermediates in response to signals from the environment. These signals constitute 'nutrients' for the bacteria in most cases. Depending on the identity of the nutrient, biofilm amounts may be reduced. The nutrient may then be used for the development of both novel prevention and treatment options for biofilm-associated infectious diseases.
MeSH term(s)	Acetates/metabolism ; Animals ; Anti-Bacterial Agents/pharmacology ; Biofilms ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli/physiology ; Escherichia coli Infections/drug therapy ; Escherichia coli Infections/microbiology ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Humans ; Signal Transduction/drug effects
Chemical Substances	Acetates ; Anti-Bacterial Agents ; Escherichia coli Proteins
Language	English
Publishing date	2013-07
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	752343-9
ISSN	1574-6968 ; 0378-1097
ISSN (online)	1574-6968
ISSN	0378-1097
DOI	10.1111/1574-6968.12174
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Article: Fighting Post-COVID and ME/CFS - development of curative therapies.

Scheibenbogen, Carmen / Bellmann-Strobl, Judith Theresia / Heindrich, Cornelia / Wittke, Kirsten / Stein, Elisa / Franke, Christiana / Prüss, Harald / Preßler, Hannah / Machule, Marie-Luise / Audebert, Heinrich / Finke, Carsten / Zimmermann, Hanna Gwendolyn / Sawitzki, Birgit / Meisel, Christian / Toelle, Markus / Krueger, Anne / Aschenbrenner, Anna C / Schultze, Joachim L / Beyer, Marc D /

Ralser, Markus / Mülleder, Michael / Sander, Leif Erik / Konietschke, Frank / Paul, Friedemann / Stojanov, Silvia / Bruckert, Lisa / Hedderich, Dennis M / Knolle, Franziska / Riemekasten, Gabriela / Vehreschild, Maria J G T / Cornely, Oliver A / Behrends, Uta / Burock, Susen

Frontiers in medicine

2023 Volume 10, Page(s) 1194754

Abstract: The sequela of COVID-19 include a broad spectrum of symptoms that fall under the umbrella term post-COVID-19 condition or syndrome (PCS). Immune dysregulation, autoimmunity, endothelial dysfunction, viral persistence, and viral reactivation have been ... ...

Abstract	The sequela of COVID-19 include a broad spectrum of symptoms that fall under the umbrella term post-COVID-19 condition or syndrome (PCS). Immune dysregulation, autoimmunity, endothelial dysfunction, viral persistence, and viral reactivation have been identified as potential mechanisms. However, there is heterogeneity in expression of biomarkers, and it is unknown yet whether these distinguish different clinical subgroups of PCS. There is an overlap of symptoms and pathomechanisms of PCS with postinfectious myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). No curative therapies are available for ME/CFS or PCS. The mechanisms identified so far provide targets for therapeutic interventions. To accelerate the development of therapies, we propose evaluating drugs targeting different mechanisms in clinical trial networks using harmonized diagnostic and outcome criteria and subgrouping patients based on a thorough clinical profiling including a comprehensive diagnostic and biomarker phenotyping.
Language	English
Publishing date	2023-06-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2023.1194754
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Article ; Online: Spontaneous mutations in the flhD operon generate motility heterogeneity in Escherichia coli biofilm.

Horne, Shelley M / Sayler, Joseph / Scarberry, Nicholas / Schroeder, Meredith / Lynnes, Ty / Prüß, Birgit M

BMC microbiology

2016 Volume 16, Issue 1, Page(s) 262

Abstract: Background: Heterogeneity and niche adaptation in bacterial biofilm involve changes to the genetic makeup of the bacteria and gene expression control. We hypothesized that i) spontaneous mutations in the flhD operon can either increase or decrease ... ...

Abstract	Background: Heterogeneity and niche adaptation in bacterial biofilm involve changes to the genetic makeup of the bacteria and gene expression control. We hypothesized that i) spontaneous mutations in the flhD operon can either increase or decrease motility and that ii) the resulting motility heterogeneity in the biofilm might lead to a long-term increase in biofilm biomass. Results: We allowed the highly motile E. coli K-12 strain MC1000 to form seven- and fourteen-day old biofilm, from which we recovered reduced motility isolates at a substantially greater frequency (5.4 %) than from a similar experiment with planktonic bacteria (0.1 %). Biofilms formed exclusively by MC1000 degraded after 2 weeks. In contrast, biofilms initiated with a 1:1 ratio of MC1000 and its isogenic flhD::kn mutant remained intact at 4 weeks and the two strains remained in equilibrium for at least two weeks. These data imply that an 'optimal' biofilm may contain a mixture of motile and non-motile bacteria. Twenty-eight of the non-motile MC1000 isolates contained an IS1 element in proximity to the translational start of FlhD or within the open reading frames for FlhD or FlhC. Two isolates had an IS2 and one isolate had an IS5 in the open reading frame for FlhD. An additional three isolates contained deletions that included the RNA polymerase binding site, five isolates contained point mutations and small deletions in the open reading frame for FlhC. The locations of all these mutations are consistent with the lack of motility and further downstream within the flhD operon than previously published IS elements that increased motility. We believe that the location of the mutation within the flhD operon determines whether the effect on motility is positive or negative. To test the second part of our hypothesis where motility heterogeneity in a biofilm may lead to a long-term increase in biofilm biomass, we quantified biofilm biomass by MC1000, MC1000 flhD::kn, and mixtures of the two strains at ratios of 1:1, 10:1, and 1:10. After 3 weeks, biofilm of the mixed cultures contained up to five times more biomass than biofilm of each of the individual strains. Conclusion: Mutations in the flhD operon can exert positive or negative effects on motility, depending on the site of the mutation. We believe that this is a mechanism to generate motility heterogeneity within E. coli biofilm, which may help to maintain biofilm biomass over extended periods of time.
MeSH term(s)	Biofilms ; Escherichia coli/genetics ; Escherichia coli/physiology ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Gene Expression Regulation, Bacterial ; Mutation ; Operon ; Trans-Activators/genetics ; Trans-Activators/metabolism
Chemical Substances	Escherichia coli Proteins ; Trans-Activators ; flhD protein, E coli
Language	English
Publishing date	2016-11-08
Publishing country	England
Document type	Journal Article
ISSN	1471-2180
ISSN (online)	1471-2180
DOI	10.1186/s12866-016-0878-1
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