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Article ; Online: Penser l’école par le vécu des acteurs en contextes arabes et musulmans

Chloé Pellegrini

Revue des Mondes Musulmans et de la Méditerranée, Vol 154, Pp 9-

2023 Volume 16

Keywords	History of Africa ; DT1-3415 ; Social sciences (General) ; H1-99
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Université de Provence
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Drought stress modulates secondary metabolites in Brassica oleracea L. convar. acephala (DC) Alef, var. sabellica L.

Podda, Alessandra / Pollastri, Susanna / Bartolini, Paola / Pisuttu, Claudia / Pellegrini, Elisa / Nali, Cristina / Cencetti, Gabriele / Michelozzi, Marco / Frassinetti, Stefania / Giorgetti, Lucia / Fineschi, Silvia / Del Carratore, Renata / Maserti, Biancaelena

Journal of the science of food and agriculture

2019 Volume 99, Issue 12, Page(s) 5533–5540

Abstract: ... kale Brassica oleracea L. convar. Acephala (DC) var. sabellica to drought stress and the impact ...

Abstract	Background: Consumer preference today is for the consumption of functional food and the reduction of chemical preservatives. Moreover, the antimicrobial properties and health-promoting qualities of plant secondary metabolites are well known. Due to forecasted climate changes and increasing human population, agricultural practices for saving water have become a concern. In the present study, the physiological responses of curly kale Brassica oleracea L. convar. Acephala (DC) var. sabellica to drought stress and the impact of water limitation on the concentration of selected secondary metabolites were investigated under laboratory-controlled conditions. Results: Results indicated that drought stress increased the content of trans-2-hexenal, phytol and δ-tocopherol, and decreased chlorophyll content. Moreover, drought stress increased antioxidant capacity and the expression of AOP2, a gene associated with the biosynthesis of aliphatic alkenyl glucosinolates, and of three genes - TGG1, TGGE and PEN2 - encoding for myrosinases, the enzymes involved in glucosinolate breakdown. Conclusion: The present study shows that water limitation during the growing phase might be exploited as a sustainable practice for producing curly kale with a high concentration of nutritionally important health-promoting bioactive metabolites. © 2019 Society of Chemical Industry.
MeSH term(s)	Antioxidants/analysis ; Antioxidants/metabolism ; Brassica/chemistry ; Brassica/metabolism ; Droughts ; Glucosinolates/analysis ; Glucosinolates/metabolism ; Secondary Metabolism
Chemical Substances	Antioxidants ; Glucosinolates
Language	English
Publishing date	2019-07-02
Publishing country	England
Document type	Journal Article
ZDB-ID	184116-6
ISSN	1097-0010 ; 0022-5142
ISSN (online)	1097-0010
ISSN	0022-5142
DOI	10.1002/jsfa.9816
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Article ; Online: Turmeric (Curcuma longa L.) food supplements and hepatotoxicity: an integrated evaluation approach.

Menniti-Ippolito, Francesca / Ippoliti, Ilaria / Pastorelli, Augusto Alberto / Altieri, Ilaria / Scalise, Francesco / De Santis, Barbara / Debegnach, Francesca / Brera, Carlo / Pacifici, Roberta / Pichini, Simona / Pellegrini, Manuela / Rotolo, Maria Concetta / Graziano, Silvia / Palazzino, Giovanna / Multari, Giuseppina / Gallo, Francesca Romana / Neri, Bruno / Giannetti, Luigi / Russo, Katia /

Fedrizzi, Giorgio / Bonan, Stefania / Mazzanti, Gabriela / Moro, Paola Angela / Salvi, Emanuela / Firenzuoli, Fabio / Valeri, Andrea / Moretti, Ugo / Traversa, Giuseppe / Silano, Marco / Stacchini, Paolo / Boniglia, Concetta

Annali dell'Istituto superiore di sanita

2020 Volume 56, Issue 4, Page(s) 462–469

Abstract: Introduction: Turmeric is the common name for the rhizome of Curcuma longa L. In the recent years ...

Abstract	Introduction: Turmeric is the common name for the rhizome of Curcuma longa L. In the recent years, food supplements containing turmeric have been marketed and widely used by an increasing number of consumers. Spontaneous reports of suspected adverse reactions to food supplements are collected within the Phytovigilance system. Methods: An ad hoc multidisciplinary group investigated the suspected cases of hepatotoxicity reported to the Italian Phytovigilance system associated with the assumption of turmeric food supplements with the methodology specific to pharmacovigilance as well as for the evaluation of the quality and safety of food supplements. Results: A cluster of 28 spontaneous reports of acute hepatitis, mostly with cholestasis, associated with turmeric products were sent to the Italian Phytovigilance system in the first six months of 2019. In all cases, except one, the causality assessment was at least possible. The suspected products were collected and analysed for the presence of drugs, heavy metals, aflatoxins, pesticides, synthetic dyes and pyrrolizidine alkaloids. Conclusion: On the basis of the results of all the activities performed by multidisciplinary group, regulatory intervention was taken. This study highlights the importance of developing an integrated evaluation approach for the evaluation of the adverse effects associated with the use of food supplements.
MeSH term(s)	Adult ; Aged ; Chemical and Drug Induced Liver Injury/etiology ; Curcuma/adverse effects ; Dietary Supplements/adverse effects ; Female ; Humans ; Italy ; Male ; Middle Aged ; Plant Extracts/adverse effects
Chemical Substances	Plant Extracts ; turmeric extract (856YO1Z64F)
Language	English
Publishing date	2020-12-21
Publishing country	Italy
Document type	Journal Article
ZDB-ID	950344-4
ISSN	2384-8553 ; 0021-2571
ISSN (online)	2384-8553
ISSN	0021-2571
DOI	10.4415/ANN_20_04_08
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Article ; Online: Biocontrol of Soil-Borne Pathogens of Solanum lycopersicum L. and Daucus carota L. by Plant Growth-Promoting Actinomycetes

Rihab Djebaili / Marika Pellegrini / Claudia Ercole / Beatrice Farda / Mahmoud Kitouni / Maddalena Del Gallo

Pathogens, Vol 10, Iss 1305, p

In Vitro and In Planta Antagonistic Activity

2021 Volume 1305

Abstract: Biotic stress caused by pathogenic microorganisms leads to damage in crops. Tomato and carrot are among the most important vegetables cultivated worldwide. These plants are attacked by several pathogens, affecting their growth and productivity. Fourteen ... ...

Abstract	Biotic stress caused by pathogenic microorganisms leads to damage in crops. Tomato and carrot are among the most important vegetables cultivated worldwide. These plants are attacked by several pathogens, affecting their growth and productivity. Fourteen plant growth-promoting actinomycetes (PGPA) were screened for their in vitro biocontrol activity against Solanum lycopersicum and Daucus carota microbial phytopathogens. Their antifungal activity was evaluated against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and Rhizoctonia solani (RHS). Antibacterial activity was evaluated against Pseudomonas syringae , Pseudomonas corrugata , Pseudomonas syringae pv. actinidiae , and Pectobacterium carotovorum subsp. carotovorum . Strains that showed good in vitro results were further investigated in vitro (cell-free supernatants activity, scanning electron microscope observations of fungal inhibition). The consortium of the most active PGPA was then utilized as biocontrol agents in planta experiments on S. lycopersicum and D. carota . The Streptomyces albidoflavus H12 and Nocardiopsis aegyptica H14 strains showed the best in vitro biocontrol activities. The diffusible and volatile compounds and cell-free supernatants of these strains showed both antifungal (in vitro inhibition up to 85%, hyphal desegregation and fungicidal properties) and antibacterial activity (in vitro inhibition >25 mm and bactericidal properties). Their consortium was also able to counteract the infection symptoms of microbial phytopathogens during in planta experiments, improving plant status. The results obtained highlight the efficacy of the selected actinomycetes strains as biocontrol agents of S. lycopersicum and D. carota .
Keywords	PGPB ; actinomycetes ; antifungal activity ; antibacterial activity ; cell culture filtrate ; SEM ; Medicine ; R
Subject code	571
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Antibacterial and Biofilm Production Inhibition Activity of Thymus vulgaris L. Essential Oil against Salmonella spp. Isolates from Reptiles

Michela Galgano / Daniela Mrenoshki / Francesco Pellegrini / Loredana Capozzi / Marco Cordisco / Laura Del Sambro / Adriana Trotta / Michele Camero / Maria Tempesta / Domenico Buonavoglia / Piero Laricchiuta / Cristiana Catella / Annamaria Pratelli / Alessio Buonavoglia / Marialaura Corrente

Pathogens, Vol 12, Iss 804, p

2023 Volume 804

Abstract: ... the efficacy of Thymus vulgaris L. essential oil (TEO) in inhibiting bacterial growth and biofilm production ...

Abstract	Salmonellosis is an infectious disease affecting both animals and humans. Antimicrobial resistant (AMR) and biofilm-producing Salmonella spp., frequently detected in reptiles (who can then act as asymptomatic carriers for warm-blooded animals), have developed resistance to biocides; this represents a warning for the emergence of biocide/antimicrobial cross-resistance. The aim of this study was to evaluate the efficacy of Thymus vulgaris L. essential oil (TEO) in inhibiting bacterial growth and biofilm production of Salmonella spp., which had been isolated from wild reptiles housed in a Zoo in Italy. The resistance profile against different classes of antibiotics showed that all the isolates were susceptible to the tested antibiotics, despite the presence of several AMR genes. All the isolates were also tested with aqueous solutions of TEO at different dilutions (5% to 0.039%). Interestingly, TEO proved effective both in inhibiting bacterial growth at low dilutions, with MIC and MBC values ranging between 0.078% and 0.312%, and in inhibiting biofilm production, with values ranging from 0.039% to 0.156%. TEO demonstrated effective bioactivity against the biofilm producer Salmonella spp., proving to be a valid disinfectant for the prevention of salmonellosis from reptiles, a possible source of infection for humans exposed to the reptiles’ environment.
Keywords	Salmonella ; reptiles ; Thymus vulgaris ; antibiotic resistance ; Medicine ; R
Subject code	630 ; 616
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria

Marika Pellegrini / Daniela M. Spera / Claudia Ercole / Maddalena Del Gallo

Microorganisms, Vol 9, Iss 639, p

Effects on Plants, Soil, and the Autochthonous Microbial Community

2021 Volume 639

Abstract: ... on Allium cepa L. and on soil health. The bacterial consortium was inoculated on seeds of two different onion ...

Abstract	The present work was aimed at investigating the effects of a four bacterial strain consortium— Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae , and Burkholderia ambifaria —on Allium cepa L. and on soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated and Control seeds (treated with autoclaved inoculum) were sown in an open-field and followed until harvest. Plant growth development parameters, as well as soil physico–chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulb dry matter (+3%) with respect to the Control. The differences between Control and treatments were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase in total organic carbon, organic matter, and available phosphorus, as well as higher concentrations of nutrients than the Control. The ecological indexes calculated from the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work showed the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes whilst safeguarding soil biodiversity.
Keywords	biostimulants ; PGPB ; seed inoculation ; sustainable agriculture ; illumina sequencing ; Biology (General) ; QH301-705.5
Subject code	630 ; 580
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria

Marika Pellegrini / Daniela M. Spera / Claudia Ercole / Maddalena del Gallo

Proceedings, Vol 66, Iss 1, p

Effects on Plant Growth and Development and Soil Fertility Status and Microbial Community

2021 Volume 20

Abstract: ... on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two ...

Abstract	The present work was aimed at investigating the effects of a four strains consortium— Azospirillum brasilense , Gluconacetobacter diazotrophicus , Herbaspirillum seropedicae , and Burkholderia ambifaria —on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated seeds and control ones (treated with autoclaved inoculum) were sown in open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulbs dry matter (+3%) than the control. The differences between control and treated experimental conditions were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase of total organic carbon, organic matter, and available P and higher concentrations of nutrients than control. The ecological indexes calculated on the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work allowed to remark the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes, whilst safeguarding soil biodiversity.
Keywords	biostimulants ; PGPB ; seed inoculation ; sustainable agriculture ; Illumina sequencing ; General Works ; A
Subject code	630 ; 580
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: CCL2/MCP-1 and CXCL12/SDF-1 blockade by L-aptamers improve pancreatic islet engraftment and survival in mouse.

Citro, Antonio / Pellegrini, Silvia / Dugnani, Erica / Eulberg, Dirk / Klussmann, Sven / Piemonti, Lorenzo

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

2019 Volume 19, Issue 11, Page(s) 3131–3138

Abstract: ... after islet transplantation. L-aptamers are chemically synthesized, nonimmunogenic bio-stable ...

Abstract	The blockade of pro-inflammatory mediators is a successful approach to improve the engraftment after islet transplantation. L-aptamers are chemically synthesized, nonimmunogenic bio-stable oligonucleotides that bind and inhibit target molecules conceptually similar to antibodies. We aimed to evaluate if blockade-aptamer-based inhibitors of C-C Motif Chemokine Ligand 2/monocyte chemoattractant protein-1 (CCL2/MCP-1) and C-X-C Motif Chemokine Ligand 12/stromal cell-derived factor-1 (CXCL12/SDF-1) are able to favor islet survival in mouse models for islet transplantation and for type 1 diabetes. We evaluated the efficacy of the CCL2-specific mNOX-E36 and the CXCL12-specific NOX-A12 on islet survival in a syngeneic mouse model of intraportal islet transplantation and in a multiple low doses of streptozotocin (MLD-STZ) diabetes induction model. Moreover, we characterized intrahepatic infiltrated leukocytes by flow cytometry before and 3 days after islet infusion in presence or absence of these inhibitors. The administration for 14 days of mNOX-E36 and NOX-A12 significantly improved islet engraftment, either compound alone or in combination. Intrahepatic islet transplantation recruited CD45
MeSH term(s)	Animals ; Aptamers, Nucleotide/administration & dosage ; Aptamers, Nucleotide/genetics ; Chemokine CCL2/antagonists & inhibitors ; Chemokine CCL2/genetics ; Chemokine CXCL12/antagonists & inhibitors ; Chemokine CXCL12/genetics ; Combined Modality Therapy ; Diabetes Mellitus, Experimental/pathology ; Diabetes Mellitus, Experimental/therapy ; Diabetes Mellitus, Type 1/pathology ; Diabetes Mellitus, Type 1/therapy ; Graft Survival ; Islets of Langerhans/cytology ; Islets of Langerhans Transplantation/methods ; Male ; Mice ; Mice, Inbred C57BL
Chemical Substances	Aptamers, Nucleotide ; Ccl2 protein, mouse ; Chemokine CCL2 ; Chemokine CXCL12 ; Cxcl12 protein, mouse ; NOX-A12
Language	English
Publishing date	2019-07-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2060594-8
ISSN	1600-6143 ; 1600-6135
ISSN (online)	1600-6143
ISSN	1600-6135
DOI	10.1111/ajt.15518
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Article ; Online: In vitro studies of Annona muricata L. extract-loaded electrospun scaffolds for localized treatment of breast cancer.

Akpan, Udom M / Pellegrini, Michael / Salifu, Ali A / Obayemi, John D / Ezenwafor, Theresa / Browe, Daniel / Ani, Chukwuemeka J / Danyuo, Yiporo / Dozie-Nwachukwu, Stella / Odusanya, Olushola S / Freeman, Joseph / Soboyejo, Winston O

Journal of biomedical materials research. Part B, Applied biomaterials

2021 Volume 109, Issue 12, Page(s) 2041–2056

Abstract: This paper presents in vitro studies of the sustained release of Annona muricata leaf extracts (AME) from hybrid electrospun fibers for breast cancer treatment. Electrospun hybrid scaffolds were fabricated from crude AME extracts, poly(lactic-co-glycolic ...

Abstract	This paper presents in vitro studies of the sustained release of Annona muricata leaf extracts (AME) from hybrid electrospun fibers for breast cancer treatment. Electrospun hybrid scaffolds were fabricated from crude AME extracts, poly(lactic-co-glycolic acid)/gelatin (PLGA/Ge) and pluronic F127. The physicochemical properties of the AME extract and scaffolds were studied. The antiproliferative effects of the scaffolds were also assessed on breast cancer (MCF-7 and MDA-MB-231) and non-tumorigenic breast (MCF10A) cell lines. Scanning electron microscope micrographs revealed a random network of micro- and submicron fibers. In vitro drug release profiles, governed by quasi-Fickian diffusion at pH 7.4 and non-Fickian super case II at pH 6.7, showed initial burst AME release from the PLGA/Ge-AME and PLGA/Ge-F127/AME fibers at pH 7.4, and burst release from PLGA/Ge-F127/AME (not observed from PLGA/Ge-AME) at pH 6.7. Then, a slower, sustained release of the remaining AME from the fibers, attributed to the onset of degradation of the PLGA/Ge backbone, was observed for the next 72 hr. The cumulative release of AME was 89.33 ± 0.73% (PLGA/Ge-AME) and 51.17 ± 7.96% (PLGA/Ge-F127/AME) at pH 7.4, and 9.27 ± 2.3% and 73.5 ± 4.5%, respectively, at pH 6.7. Pluronic F127 addition increased the drug loading capacity and prolonged the sustained AME release from the fibers. The released AME significantly inhibited the in vitro growth of the breast cancer cells more than the non-tumorigenic cells, due to the induction of apoptosis, providing evidence for using pluronic F127-containing electrospun fibers for sustained and localized AME delivery to breast cancer cells.
MeSH term(s)	Annona ; Breast Neoplasms/drug therapy ; Drug Liberation ; Female ; Humans ; Poloxamer/chemistry ; Poloxamer/pharmacology ; Polylactic Acid-Polyglycolic Acid Copolymer/chemistry
Chemical Substances	Poloxamer (106392-12-5) ; Polylactic Acid-Polyglycolic Acid Copolymer (1SIA8062RS)
Language	English
Publishing date	2021-05-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2099992-6
ISSN	1552-4981 ; 1552-4973 ; 0021-9304
ISSN (online)	1552-4981
ISSN	1552-4973 ; 0021-9304
DOI	10.1002/jbm.b.34852
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Article: Biocontrol of Soil-Borne Pathogens of Solanum lycopersicum L. and Daucus carota L. by Plant Growth-Promoting Actinomycetes: In Vitro and In Planta Antagonistic Activity

Djebaili, Rihab / Pellegrini, Marika / Ercole, Claudia / Farda, Beatrice / Kitouni, Mahmoud / Del Gallo, Maddalena

Pathogens. 2021 Oct. 12, v. 10, no. 10

2021

Abstract	Biotic stress caused by pathogenic microorganisms leads to damage in crops. Tomato and carrot are among the most important vegetables cultivated worldwide. These plants are attacked by several pathogens, affecting their growth and productivity. Fourteen plant growth-promoting actinomycetes (PGPA) were screened for their in vitro biocontrol activity against Solanum lycopersicum and Daucus carota microbial phytopathogens. Their antifungal activity was evaluated against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and Rhizoctonia solani (RHS). Antibacterial activity was evaluated against Pseudomonas syringae, Pseudomonas corrugata, Pseudomonas syringae pv. actinidiae, and Pectobacterium carotovorum subsp. carotovorum. Strains that showed good in vitro results were further investigated in vitro (cell-free supernatants activity, scanning electron microscope observations of fungal inhibition). The consortium of the most active PGPA was then utilized as biocontrol agents in planta experiments on S. lycopersicum and D. carota. The Streptomyces albidoflavus H12 and Nocardiopsis aegyptica H14 strains showed the best in vitro biocontrol activities. The diffusible and volatile compounds and cell-free supernatants of these strains showed both antifungal (in vitro inhibition up to 85%, hyphal desegregation and fungicidal properties) and antibacterial activity (in vitro inhibition >25 mm and bactericidal properties). Their consortium was also able to counteract the infection symptoms of microbial phytopathogens during in planta experiments, improving plant status. The results obtained highlight the efficacy of the selected actinomycetes strains as biocontrol agents of S. lycopersicum and D. carota.
Keywords	Daucus carota ; Fusarium oxysporum f. sp. radicis-lycopersici ; Nocardiopsis ; Pseudomonas mediterranea ; Pseudomonas syringae pv. actinidiae ; Solanum lycopersicum ; Streptomyces albidoflavus ; Thanatephorus cucumeris ; antibacterial properties ; antifungal properties ; biological control ; biotic stress ; carrots ; fungi ; hyphae ; plant pathogens ; tomatoes
Language	English
Dates of publication	2021-1012
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens10101305
Database	NAL-Catalogue (AGRICOLA)

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