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  1. Article: Recent progress in experimental studies on the catalytic mechanism of cytochrome

    Shimada, Atsuhiro / Tsukihara, Tomitake / Yoshikawa, Shinya

    Frontiers in chemistry

    2023  Volume 11, Page(s) 1108190

    Abstract: ... ...

    Abstract Cytochrome
    Language English
    Publishing date 2023-05-04
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2711776-5
    ISSN 2296-2646
    ISSN 2296-2646
    DOI 10.3389/fchem.2023.1108190
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  2. Article ; Online: Mechanical thrombectomy in acute in-stent thromboembolism.

    Dai, Monami / Imaoka, Yukihiro / Yoshikawa, Shinichiro / Kohyama, Shinya

    BMJ case reports

    2024  Volume 17, Issue 1

    Abstract: Following the implantation of a carotid artery stent (CAS), the aetiology of in-stent occlusion typically shifts from embolic events in the acute phase to intimal hyperplasia in the chronic phase. A man in his 70s with a history of CAS implantation ( ... ...

    Abstract Following the implantation of a carotid artery stent (CAS), the aetiology of in-stent occlusion typically shifts from embolic events in the acute phase to intimal hyperplasia in the chronic phase. A man in his 70s with a history of CAS implantation (performed 5 years ago) for left internal carotid artery (ICA) stenosis was admitted to our hospital with a chief complaint of left-sided transient scotoma. MRI revealed an acute occlusion of the left ICA, and an urgent digital subtraction angiography (DSA) was performed. The DSA results suggested the presence of an in-stent thrombus instead of in-stent stenosis. An urgent endovascular thrombectomy was performed, and the thrombus was successfully retrieved from the carotid stent. Full reperfusion was achieved, and no damage occurred to the carotid stent. This case highlights the importance of thorough examination of MRI and DSA findings for accurate differentiation between the causes of vessel occlusion.
    MeSH term(s) Male ; Animals ; Humans ; Constriction, Pathologic ; Thromboembolism ; Carotid Stenosis ; Coleoptera ; Stents ; Thrombectomy ; Thrombosis
    Language English
    Publishing date 2024-01-23
    Publishing country England
    Document type Case Reports ; Journal Article
    ISSN 1757-790X
    ISSN (online) 1757-790X
    DOI 10.1136/bcr-2023-257860
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  3. Article ; Online: Modularly Built Synthetic Membraneless Organelles Enabling Targeted Protein Sequestration and Release.

    Yoshikawa, Masaru / Tsukiji, Shinya

    Biochemistry

    2021  Volume 60, Issue 44, Page(s) 3273–3276

    MeSH term(s) Animals ; Artificial Cells/chemistry ; Artificial Cells/metabolism ; Biomolecular Condensates/chemistry ; Biomolecular Condensates/metabolism ; Humans ; Protein Engineering/methods ; Synthetic Biology/methods
    Language English
    Publishing date 2021-10-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1108-3
    ISSN 1520-4995 ; 0006-2960
    ISSN (online) 1520-4995
    ISSN 0006-2960
    DOI 10.1021/acs.biochem.1c00675
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 217: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article ; Online: Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri.

    Takeuchi, Yu / Sato, Shinya / Nagasato, Chikako / Motomura, Taizo / Okuda, Shujiro / Kasahara, Masahiro / Takahashi, Fumio / Yoshikawa, Shinya

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 3387

    Abstract: Spermatogenesis is one of the most dramatic changes in cell differentiation. Remarkable chromatin condensation of the nucleus is observed in animal, plant, and algal sperm. Sperm nuclear basic proteins (SNBPs), such as protamine and sperm-specific ... ...

    Abstract Spermatogenesis is one of the most dramatic changes in cell differentiation. Remarkable chromatin condensation of the nucleus is observed in animal, plant, and algal sperm. Sperm nuclear basic proteins (SNBPs), such as protamine and sperm-specific histone, are involved in chromatin condensation of the sperm nucleus. Among brown algae, sperm of the oogamous Fucales algae have a condensed nucleus. However, the existence of sperm-specific SNBPs in Fucales algae was unclear. Here, we identified linker histone (histone H1) proteins in the sperm and analyzed changes in their gene expression pattern during spermatogenesis in Sargassum horneri. A search of transcriptomic data for histone H1 genes in showed six histone H1 genes, which we named ShH1.1a, ShH1b, ShH1.2, ShH1.3, ShH1.4, and ShH1.5. Analysis of SNBPs using SDS-PAGE and LC-MS/MS showed that sperm nuclei contain histone ShH1.2, ShH1.3, and ShH1.4 in addition to core histones. Both ShH1.2 and ShH1.3 genes were expressed in the vegetative thallus and the male and female receptacles (the organs producing antheridium or oogonium). Meanwhile, the ShH1.4 gene was expressed in the male receptacle but not in the vegetative thallus and female receptacles. From these results, ShH1.4 may be a sperm-specific histone H1 of S. horneri.
    MeSH term(s) Animals ; Male ; Histones/genetics ; Histones/metabolism ; Sargassum/metabolism ; Chromatography, Liquid ; Semen/metabolism ; Tandem Mass Spectrometry ; Cell Nucleus/metabolism ; Chromatin/metabolism ; Spermatozoa/metabolism
    Chemical Substances Histones ; Chromatin
    Language English
    Publishing date 2024-02-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-53729-2
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  5. Article: Reversed-Image Mechanical Thrombectomy for Acute Ischemic Stroke in a Situs Inversus Totalis Patient.

    Tamada, Keita / Sato, Hiroki / Iihoshi, Satoshi / Imaoka, Yukihiro / Yoshikawa, Shinichiro / Kurita, Hiroki / Kohyama, Shinya

    Cureus

    2024  Volume 16, Issue 3, Page(s) e55629

    Abstract: An 85-year-old female with situs inversus totalis presented with right hemiplegia, right facial nerve palsy, eye deviation to the left, and aphasia. Magnetic resonance imaging revealed acute ischemic lesions in the left insular cortex and the frontal ... ...

    Abstract An 85-year-old female with situs inversus totalis presented with right hemiplegia, right facial nerve palsy, eye deviation to the left, and aphasia. Magnetic resonance imaging revealed acute ischemic lesions in the left insular cortex and the frontal lobe. Magnetic resonance angiography revealed an occlusion of the left internal carotid artery. Reversed-image mechanical thrombectomy achieved complete reperfusion in three passes within 54 minutes. Six months post-intervention, the patient could walk indoors independently. Our technique, which replicates the normal arterial anatomy by inversion and angulation, was adapted to situs inversus totalis.
    Language English
    Publishing date 2024-03-06
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.55629
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  6. Article ; Online: The cAMP signaling module regulates sperm motility in the liverwort

    Yamamoto, Chiaki / Takahashi, Fumio / Suetsugu, Noriyuki / Yamada, Kazumasa / Yoshikawa, Shinya / Kohchi, Takayuki / Kasahara, Masahiro

    Proceedings of the National Academy of Sciences of the United States of America

    2024  Volume 121, Issue 16, Page(s) e2322211121

    Abstract: Adenosine 3',5'-cyclic monophosphate (cAMP) is a universal signaling molecule that acts as a second messenger in various organisms. It is well established that cAMP plays essential roles across the tree of life, although the function of cAMP in land ... ...

    Abstract Adenosine 3',5'-cyclic monophosphate (cAMP) is a universal signaling molecule that acts as a second messenger in various organisms. It is well established that cAMP plays essential roles across the tree of life, although the function of cAMP in land plants has long been debated. We previously identified the enzyme with both adenylyl cyclase (AC) and cAMP phosphodiesterase (PDE) activity as the cAMP-synthesis/hydrolysis enzyme COMBINED AC with PDE (CAPE) in the liverwort
    MeSH term(s) Male ; Animals ; Marchantia/genetics ; Cyclic AMP/metabolism ; Sperm Motility/genetics ; Seeds/metabolism ; Adenylyl Cyclases/metabolism ; Spermatozoa/metabolism
    Chemical Substances Cyclic AMP (E0399OZS9N) ; Adenylyl Cyclases (EC 4.6.1.1)
    Language English
    Publishing date 2024-04-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2322211121
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  7. Article ; Online: A Peptide Nanocage Constructed by Self-Assembly of Oligoproline Conjugates.

    Matsubara, Shogo / Okamoto, Yui / Yoshikawa, Masaru / Tsukiji, Shinya / Higuchi, Masahiro

    Bioconjugate chemistry

    2022  Volume 33, Issue 10, Page(s) 1785–1788

    Abstract: Cage-like supramolecular assemblies called molecular cages, which possess attractive functions, have been prepared. Although biomolecule-based nanocages are required for biological/medical applications such as drug delivery systems, the majority of ... ...

    Abstract Cage-like supramolecular assemblies called molecular cages, which possess attractive functions, have been prepared. Although biomolecule-based nanocages are required for biological/medical applications such as drug delivery systems, the majority of nanocages are constructed using aromatic compounds with lower biocompatibility and biodegradability. In this study, the construction of a peptide nanocage consisting of an oligoproline conjugate is demonstrated. The conjugate was easy to prepare and had high biocompatibility. The oligoproline moiety of the conjugate had a rigid, rod-like structure suitable for the backbone of the supramolecular nanocage. The conjugates self-assembled to form peptide nanocages with a huge inner cavity.
    MeSH term(s) Peptides/chemistry ; Drug Delivery Systems
    Chemical Substances Peptides
    Language English
    Publishing date 2022-07-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1024041-x
    ISSN 1520-4812 ; 1043-1802
    ISSN (online) 1520-4812
    ISSN 1043-1802
    DOI 10.1021/acs.bioconjchem.2c00290
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3400: Show issues Location:
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  8. Article ; Online: Searching for new molecules involved in Anopheles mosquitoes' response to Plasmodium infection.

    Haraguchi, Asako / Takano, Makoto / Fujiwara, Kanta / Hakozaki, Jun / Nakayama, Kazuhiko / Nakamura, Sakure / Yoshikawa, Yasunaga / Fukumoto, Shinya / Kusakisako, Kodai / Ikadai, Hiromi

    The Journal of veterinary medical science

    2024  Volume 86, Issue 5, Page(s) 485–492

    Abstract: Plasmodium parasites within mosquitoes are exposed to various physiological processes, such as blood meal digestion activity, the gonotrophic cycle, and host responses preventing the entry of parasites into the midgut wall. However, when in vitro- ... ...

    Abstract Plasmodium parasites within mosquitoes are exposed to various physiological processes, such as blood meal digestion activity, the gonotrophic cycle, and host responses preventing the entry of parasites into the midgut wall. However, when in vitro-cultured ookinetes are injected into the hemocoel of mosquitoes, Plasmodium parasites are not affected by the vertebrate host's blood contents and do not pass through the midgut epithelial cells. This infection method might aid in identifying mosquito-derived factors affecting Plasmodium development within mosquitoes. This study investigated novel mosquito-derived molecules related to parasite development in Anopheles mosquitoes. We injected in vitro-cultured Plasmodium berghei (ANKA strain) ookinetes into female and male Anopheles stephensi (STE2 strain) mosquitoes and found that the oocyst number was significantly higher in males than in females, suggesting that male mosquitoes better support the development of parasites. Next, RNA-seq analysis was performed on the injected female and male mosquitoes to identify genes exhibiting changes in expression. Five genes with different expression patterns between sexes and greatest expression changes were identified as being potentially associated with Plasmodium infection. Two of the five genes also showed expression changes with infection by blood-feeding, indicating that these genes could affect the development of Plasmodium parasites in mosquitoes.
    MeSH term(s) Animals ; Anopheles/parasitology ; Female ; Male ; Plasmodium berghei/physiology ; Malaria/parasitology ; Mosquito Vectors/parasitology ; Mice ; Host-Parasite Interactions
    Language English
    Publishing date 2024-04-03
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1071753-5
    ISSN 1347-7439 ; 0916-7250
    ISSN (online) 1347-7439
    ISSN 0916-7250
    DOI 10.1292/jvms.24-0008
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 579: Show issues Location:
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  9. Article ; Online: Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells.

    Yoshikawa, Masaru / Yoshii, Tatsuyuki / Ikuta, Masahiro / Tsukiji, Shinya

    Journal of the American Chemical Society

    2021  Volume 143, Issue 17, Page(s) 6434–6446

    Abstract: Compartmentation of proteins into biomolecular condensates or membraneless organelles formed by phase separation is an emerging principle for the regulation of cellular processes. Creating synthetic condensates that accommodate specific intracellular ... ...

    Abstract Compartmentation of proteins into biomolecular condensates or membraneless organelles formed by phase separation is an emerging principle for the regulation of cellular processes. Creating synthetic condensates that accommodate specific intracellular proteins on demand would have various applications in chemical biology, cell engineering, and synthetic biology. Here, we report the construction of synthetic protein condensates capable of recruiting and/or releasing proteins of interest in living mammalian cells in response to a small molecule or light. By a modular combination of a tandem fusion of two oligomeric proteins, which forms phase-separated synthetic protein condensates in cells, with a chemically induced dimerization tool, we first created a chemogenetic protein condensate system that can rapidly recruit target proteins from the cytoplasm to the condensates by addition of a small-molecule dimerizer. We next coupled the protein-recruiting condensate system with an engineered proximity-dependent protease, which gave a second protein condensate system wherein target proteins previously expressed inside the condensates are released into the cytoplasm by small-molecule-triggered protease recruitment. Furthermore, an optogenetic condensate system that allows reversible release and sequestration of protein activity in a repeatable manner using light was constructed successfully. These condensate systems were applicable to control protein activity and cellular processes such as membrane ruffling and ERK signaling in a time scale of minutes. This proof-of-principle work provides a new platform for chemogenetic and optogenetic control of protein activity in mammalian cells and represents a step toward tailor-made engineering of synthetic protein condensate-based soft materials with various functionalities for biological and biomedical applications.
    MeSH term(s) Animals ; Artificial Cells/chemistry ; Artificial Cells/metabolism ; COS Cells ; Cell Engineering ; Chlorocebus aethiops ; HeLa Cells ; Humans ; MAP Kinase Signaling System ; Mice ; NIH 3T3 Cells ; Proteins/chemistry ; Proteins/genetics ; Proteins/metabolism ; Subcellular Fractions/metabolism
    Chemical Substances Proteins
    Language English
    Publishing date 2021-04-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3155-0
    ISSN 1520-5126 ; 0002-7863
    ISSN (online) 1520-5126
    ISSN 0002-7863
    DOI 10.1021/jacs.0c12375
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    In stock of ZB MED Bonn / Germany

    Z 4' 55/32: Show issues
    Z 7.3: Show issues

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  10. Article ; Online: Rapidly expanding cutaneous Mycobacterium chelonae infection in eosinophilic granulomatosis with polyangiitis.

    Matsushita, Shinya / Uehara, Masaaki / Adachi, Eisuke / Yoshikawa, Noritada / Yamamoto, Motohisa

    Rheumatology (Oxford, England)

    2021  Volume 61, Issue 6, Page(s) e160

    MeSH term(s) Churg-Strauss Syndrome/complications ; Churg-Strauss Syndrome/diagnosis ; Eosinophils ; Granulomatosis with Polyangiitis ; Humans ; Mycobacterium chelonae ; Skin Diseases, Bacterial/diagnosis ; Skin Diseases, Bacterial/drug therapy
    Language English
    Publishing date 2021-07-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 1464822-2
    ISSN 1462-0332 ; 1462-0324
    ISSN (online) 1462-0332
    ISSN 1462-0324
    DOI 10.1093/rheumatology/keab576
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 240: Show issues Location:
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    Zs.MO 497: Show issues

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