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Article ; Online: Measurement of enzyme activity of insoluble substrates based on ordered porous layer interferometry and the application in evaluation of thrombolytic drugs.

Su, Qianqian / Sun, Yu / Tang, Yanhua / Ni, Na / Ding, Nian

2024 Volume 149, Issue 5, Page(s) 1537–1547

Abstract: The development of innovative methods for real-time surveillance of enzymatic activity determination processes is essential, particularly for insoluble substrate enzymatic assessments. In this work, a novel method for enzymatic activity determination was ...

Abstract	The development of innovative methods for real-time surveillance of enzymatic activity determination processes is essential, particularly for insoluble substrate enzymatic assessments. In this work, a novel method for enzymatic activity determination was devised by assembling a 190 nm silica colloidal crystal (SCC) film onto a glass slide, coupled with Ordered Porous Layer Interferometry (OPLI) technology. By fixing the substrate of the enzyme on the surface of the silica sphere, a solid-liquid interface can be formed for monitoring enzymatic activity. The enzymatic activity is gauged by the change in the SCC film's thickness caused by the digestion of the loaded substrate. The procedure of chymotrypsin-mediated casein digestion was documented in real time, facilitating the examination of chymotrypsin's activity and kinetics. The newly-developed enzymatic activity determination method demonstrated exceptional sensitivity towards chymotrypsin activity, with a linear range spanning 0.0505-2.02 units per mg. Additionally, the method was extended to the assessment of fibrinolysis enzyme activity and kinetic analysis, yielding promising results. Therefore, this technique can serve as a real-time, user-friendly, cost-effective novel approach for enzymatic activity determination, providing fresh perspectives for enzymatic activity determination studies.
MeSH term(s)	Fibrinolytic Agents/pharmacology ; Chymotrypsin ; Kinetics ; Porosity ; Interferometry ; Silicon Dioxide/chemistry
Chemical Substances	Fibrinolytic Agents ; Chymotrypsin (EC 3.4.21.1) ; Silicon Dioxide (7631-86-9)
Language	English
Publishing date	2024-02-26
Publishing country	England
Document type	Journal Article
ZDB-ID	210747-8
ISSN	1364-5528 ; 0003-2654
ISSN (online)	1364-5528
ISSN	0003-2654
DOI	10.1039/d3an02054a
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Article ; Online: Materials engineering strategies for cancer vaccine adjuvant development.

Zhang, Xuanbo / Yang, Bowei / Ni, Qianqian / Chen, Xiaoyuan

Chemical Society reviews

2023 Volume 52, Issue 9, Page(s) 2886–2910

Abstract: Cancer vaccines have emerged as a powerful new tool for cancer immunotherapy. Adjuvants are vaccine ingredients that enhance the strength, velocity, and duration of the immune response. The success of adjuvants in achieving stable, safe, and immunogenic ... ...

Abstract	Cancer vaccines have emerged as a powerful new tool for cancer immunotherapy. Adjuvants are vaccine ingredients that enhance the strength, velocity, and duration of the immune response. The success of adjuvants in achieving stable, safe, and immunogenic cancer vaccines has generated enthusiasm for adjuvant development. Specifically, advances in materials science are providing insights into the rational design of vaccine adjuvants for topical cancer immunotherapy. Here, we outline the current state of materials engineering strategies, including those based on molecular adjuvants, polymers/lipids, inorganic nanoparticles, and bio-derived materials, for adjuvant development. We also elaborate on how these engineering strategies and the physicochemical features of the materials involved influence the effects of adjuvants.
MeSH term(s)	Humans ; Cancer Vaccines/therapeutic use ; Adjuvants, Vaccine ; Adjuvants, Immunologic ; Neoplasms/therapy ; Nanoparticles
Chemical Substances	Cancer Vaccines ; Adjuvants, Vaccine ; Adjuvants, Immunologic
Language	English
Publishing date	2023-05-09
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1472875-8
ISSN	1460-4744 ; 0306-0012
ISSN (online)	1460-4744
ISSN	0306-0012
DOI	10.1039/d2cs00647b
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Article ; Online: Effects of Ultrasonic Power on the Structure and Rheological Properties of Skin Collagen from Albacore (

Pan, Hao / Zhang, Xuehua / Ni, Jianbo / Liang, Qianqian / Jiang, Xin / Zhou, Zihui / Shi, Wenzheng

Marine drugs

2024 Volume 22, Issue 2

Abstract: The effects of ultrasonic power (0, 150, 300, 450, and 600 W) on the extraction yield and the structure and rheological properties of pepsin-soluble collagen (PSC) from albacore skin were investigated. Compared with the conventional pepsin extraction ... ...

Abstract	The effects of ultrasonic power (0, 150, 300, 450, and 600 W) on the extraction yield and the structure and rheological properties of pepsin-soluble collagen (PSC) from albacore skin were investigated. Compared with the conventional pepsin extraction method, ultrasonic treatment (UPSC) significantly increased the extraction yield of collagen from albacore skin, with a maximum increase of 8.56%. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that peptides of low molecular weight were produced when the ultrasonic power exceeded 300 W. Meanwhile, secondary structure, tertiary structure, and X-ray diffraction analyses showed that the original triple helix structure of collagen was intact after the ultrasonic treatment. The collagen solutions extracted under different ultrasonic powers had significant effects on the dynamic frequency sweep, but a steady shear test suggested that the collagen extracted at 150 W had the best viscosity. These results indicate that an ultrasonic power between 150 and 300 W can improve not only the extraction yield of natural collagen, but also the rheological properties of the collagen solution without compromising the triple helix structure.
MeSH term(s)	Animals ; Ultrasonics ; Pepsin A/chemistry ; Fish Proteins/chemistry ; Collagen/chemistry ; Perciformes ; Skin
Chemical Substances	Pepsin A (EC 3.4.23.1) ; Fish Proteins ; Collagen (9007-34-5)
Language	English
Publishing date	2024-02-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2175190-0
ISSN	1660-3397 ; 1660-3397
ISSN (online)	1660-3397
ISSN	1660-3397
DOI	10.3390/md22020084
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Article ; Online: Circ-EIF3I Promotes Hepatocellular Carcinoma Progression Through Modulating miR-361-3p/DUSP2 Axis.

Ni, Lingna / Gao, Qianqian / Zhao, Qiu / Dai, Kejun / Jin, Mingming / Fu, Cong / Xiao, Min / Zhu, Wenyu / Bi, Yanzhi

DNA and cell biology

2024

Abstract: Hepatocellular carcinoma (HCC) is one of the most common malignant cancers globally. Circular RNAs (circRNAs) have been implicated in the development of HCC. Previous studies have confirmed that circ-EIF3I plays an important role in the progress of lung ... ...

Abstract	Hepatocellular carcinoma (HCC) is one of the most common malignant cancers globally. Circular RNAs (circRNAs) have been implicated in the development of HCC. Previous studies have confirmed that circ-EIF3I plays an important role in the progress of lung cancer. Nevertheless, the biological functions of circ-EIF3I and the underlying mechanisms by which they regulate HCC progression remain unclear. In this study, the regulatory mechanism and targets were studied with bioinformatics analysis, luciferase reporting analysis, transwell migration, Cell Counting Kit-8, and 5-Ethynyl-2'-deoxyuridine analysis. In addition,
Language	English
Publishing date	2024-03-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	1024454-2
ISSN	1557-7430 ; 0198-0238 ; 1044-5498
ISSN (online)	1557-7430
ISSN	0198-0238 ; 1044-5498
DOI	10.1089/dna.2023.0400
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Article ; Online: Activatable NIR-II Photothermal Lipid Nanoparticles for Improved Messenger RNA Delivery.

Li, Benhao / Zhao, Mengyao / Lai, Weiping / Zhang, Xuanbo / Yang, Bowei / Chen, Xiaoyuan / Ni, Qianqian

Angewandte Chemie (International ed. in English)

2023 Volume 62, Issue 25, Page(s) e202302676

Abstract: Endosomal escape remains a central issue limiting the high protein expression of mRNA therapeutics. Here, we present second near-infrared (NIR-II) lipid nanoparticles (LNPs) containing pH activatable NIR-II dye conjugated lipid (Cy-lipid) for ... ...

Abstract	Endosomal escape remains a central issue limiting the high protein expression of mRNA therapeutics. Here, we present second near-infrared (NIR-II) lipid nanoparticles (LNPs) containing pH activatable NIR-II dye conjugated lipid (Cy-lipid) for potentiating mRNA delivery efficiency via a stimulus-responsive photothermal-promoted endosomal escape delivery (SPEED) strategy. In acidic endosomal microenvironment, Cy-lipid is protonated and turns on NIR-II absorption for light-to-heat transduction mediated by 1064 nm laser irradiation. Then, the heat-promoted LNPs morphology change triggers rapid escape of NIR-II LNPs from the endosome, allowing about 3-fold enhancement of enhanced green fluorescent protein (eGFP) encoding mRNA translation capacity compared to the NIR-II light free group. In addition, the bioluminescence intensity induced by delivered luciferase encoding mRNA in the mouse liver region shows positive correlation with incremental radiation dose, indicating the validity of the SPEED strategy.
MeSH term(s)	Mice ; Animals ; RNA, Messenger ; Liposomes ; Nanoparticles ; Lipids ; Phototherapy
Chemical Substances	Lipid Nanoparticles ; RNA, Messenger ; Liposomes ; Lipids
Language	English
Publishing date	2023-05-09
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.202302676
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Article ; Online: Similar incidence of graft glomerulonephritis in recipients with definitively diagnosed glomerulonephritis and those with unknown etiology: a retrospective observational study.

Wu, Qianqian / Ni, Xuefeng / Chen, Jingsong / Cheng, Dongrui / Zhang, Mingchao / Xie, Kenan / Li, Xue / Wen, Jiqiu

Renal failure

2024 Volume 46, Issue 1, Page(s) 2325644

Abstract: Objective: In China, most of the patients who underwent kidney transplants have unknown causes of end-stage renal disease (uESRD). However, little is known regarding the incidence of graft glomerulonephritis (GN) and graft survival in kidney transplant ... ...

Abstract	Objective: In China, most of the patients who underwent kidney transplants have unknown causes of end-stage renal disease (uESRD). However, little is known regarding the incidence of graft glomerulonephritis (GN) and graft survival in kidney transplant recipients (KTRs) with uESRD. Methods: In this retrospective cohort study, 473 of the 565 KTRs who underwent kidney transplantation (KTx) from 2015 to 2020 were included. We mainly observed the occurrence of graft GN between uESRD group and definitively diagnosed GN group, and repeatedly compared after propensity score matching (PSM). Results: The median follow-up was 50 months in 473 KTRs, and about 75% of KTRs of native kidney disease of unknown etiology. The total cumulative incidence of graft GN was 17%, and no difference was observed between the definitively diagnosed GN group and the uESRD group ( Conclusions: The incidence of graft GN was similar between uESRD and definitively diagnosed GN group. The allograft survival was also similar between two groups.
MeSH term(s)	Male ; Humans ; Incidence ; Retrospective Studies ; Glomerulonephritis/complications ; Glomerulonephritis/epidemiology ; Kidney Failure, Chronic/epidemiology ; Kidney Failure, Chronic/surgery ; China/epidemiology
Language	English
Publishing date	2024-03-06
Publishing country	England
Document type	Observational Study ; Journal Article
ZDB-ID	632949-4
ISSN	1525-6049 ; 0886-022X
ISSN (online)	1525-6049
ISSN	0886-022X
DOI	10.1080/0886022X.2024.2325644
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Zs.A 1331: Show issues

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Article ; Online: Right femoral vein and right dorsal artery thrombosis in childhood acute myeloid leukemia: A case report.

Ni, Jiaqi / Chen, Min / Su, Yali / Gao, Qianqian / Liu, Lingjun / Lu, Xiaoxi

Medicine

2023 Volume 102, Issue 41, Page(s) e35121

Abstract: Background: It is rare for newly diagnosed (de novo) or newly treated acute myeloid leukemia (AML) complicated with thrombotic complications, especially combined arterial and venous thrombosis.: Methods: We reported a 13-year-old boy diagnosed with ... ...

Abstract	Background: It is rare for newly diagnosed (de novo) or newly treated acute myeloid leukemia (AML) complicated with thrombotic complications, especially combined arterial and venous thrombosis. Methods: We reported a 13-year-old boy diagnosed with AML and leukocytosis, who developed right femoral vein and right dorsal artery thrombosis during chemotherapy. After treatment with low molecular weight heparin, diosmin, and alprostadil, symptoms were relieved. Unfortunately, the child suffered from coagulopathy afterward, which was unexpectedly caused by vitamin K deficiency. Results: After supplementation with vitamin K and prothrombin complex concentrate, coagulation function recovered. Conclusion: For childhood AML patients with high thrombotic risks, close monitoring during anticoagulant treatment was necessary. Concomitantly, we should be alert to past medication history and combined medication use, especially those that may lead to vitamin K deficiency, secondary bleeding, and coagulation disorders. Rational use of antibiotics, anticoagulants, and antitumor drugs must be guaranteed.
MeSH term(s)	Male ; Humans ; Child ; Adolescent ; Femoral Vein/pathology ; Anticoagulants ; Thrombosis/etiology ; Blood Coagulation Disorders/chemically induced ; Leukemia, Myeloid, Acute/complications ; Leukemia, Myeloid, Acute/drug therapy ; Leukemia, Myeloid, Acute/pathology ; Vitamin K Deficiency/complications ; Arteries
Chemical Substances	Anticoagulants
Language	English
Publishing date	2023-10-13
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000035121
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Article ; Online: Dynamic variations in and prediction of COVID-19 with omicron in the four first-tier cities of mainland China, Hong Kong, and Singapore.

Ni, Xiaohua / Sun, Bo / Hu, Zengyun / Cui, Qianqian / Zhang, Zhuo / Zhang, Hua

Frontiers in public health

2023 Volume 11, Page(s) 1228564

Abstract: Background: The COVID-19 pandemic, which began in late 2019, has resulted in the devastating collapse of the social economy and more than 10 million deaths worldwide. A recent study suggests that the pattern of COVID-19 cases will resemble a mini-wave ... ...

Abstract	Background: The COVID-19 pandemic, which began in late 2019, has resulted in the devastating collapse of the social economy and more than 10 million deaths worldwide. A recent study suggests that the pattern of COVID-19 cases will resemble a mini-wave rather than a seasonal surge. In general, COVID-19 has more severe impacts on cities than on rural areas, especially in cities with high population density. Methods: In this study, the background situation of COVID-19 transmission is discussed, including the population number and population density. Moreover, a widely used time series autoregressive integrated moving average (ARIMA) model is applied to simulate and forecast the COVID-19 variations in the six cities. We comprehensively analyze the dynamic variations in COVID-19 in the four first-tier cities of mainland China (BJ: Beijing, SH: Shanghai, GZ: Guangzhou and SZ: Shenzhen), Hong Kong (HK), China and Singapore (SG) from 2020 to 2022. Results: The major results show that the six cities have their own temporal characteristics, which are determined by the different control and prevention measures. The four first-tier cities of mainland China (i.e., BJ, SH, GZ, and SZ) have similar variations with one wave because of their identical "Dynamic COVID-19 Zero" strategy and strict Non-Pharmaceutical Interventions (NPIs). HK and SG have multiple waves primarily caused by the input cases. The ARIMA model has the ability to provide an accurate forecast of the COVID-19 pandemic trend for the six cities, which could provide a useful approach for predicting the short-term variations in infectious diseases.Accurate forecasting has significant value for implementing reasonable control and prevention measures. Conclusions: Our main conclusions show that control and prevention measures should be dynamically adjusted and organically integrated for the COVID-19 pandemic. Moreover, the mathematical models are proven again to provide an important scientific basis for disease control.
MeSH term(s)	Humans ; COVID-19/epidemiology ; China/epidemiology ; Hong Kong/epidemiology ; Cities/epidemiology ; Pandemics ; Singapore/epidemiology
Language	English
Publishing date	2023-10-10
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2711781-9
ISSN	2296-2565 ; 2296-2565
ISSN (online)	2296-2565
ISSN	2296-2565
DOI	10.3389/fpubh.2023.1228564
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Article ; Online: Association of maternal SARS-CoV-2 infection at the time of admission for delivery with labor process and outcomes of vaginal birth: A cohort study.

Chen, An / Acharya, Ganesh / Hu, Min / Gao, Xin / Cheng, Guizhi / Jiang, Lai / Ni, Qianqian

Acta obstetricia et gynecologica Scandinavica

2023 Volume 103, Issue 1, Page(s) 103–110

Abstract: Introduction: This study aimed to investigate the impact of maternal SARS-CoV-2 infection at the time of admission for delivery on labor process and outcomes of vaginal birth.: Material and methods: A cohort study was carried out at the Obstetrics ... ...

Abstract	Introduction: This study aimed to investigate the impact of maternal SARS-CoV-2 infection at the time of admission for delivery on labor process and outcomes of vaginal birth. Material and methods: A cohort study was carried out at the Obstetrics Department of Anhui Provincial Hospital, China, where universal reverse transcriptase polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 infection was introduced for all women admitted for labor and delivery from December 1-31, 2022. Women were divided into positive and negative groups based on the test result. All women having a singleton vaginal birth were included in final analysis. The effect of SARS-CoV-2 positivity on labor process and outcomes of vaginal birth was estimated by regression analyses. Results: Among a total of 360 women included, 87 had a positive SARS-CoV-2 test and 273 a negative test. Women in the positive group had an increased likelihood of having longer labor (median 9.3 vs 8.3 hours; sB [log-transformed] 0.19; 95% confidence interval [CI] 0.09-0.28), episiotomy (39.1% vs 23.8%; adjusted odds ratio [aOR] 2.31; 95% CI 1.27-4.21), grade III meconium-stained amniotic fluid (19.5% vs 7.0%; aOR 2.52; 95% CI 1.15-5.54) and postpartum hospital stay exceeding 37 hours (58.6% vs 46.5%; aOR 1.71; 95% CI 1.00-2.91). They had reduced rates exclusive breastfeeding (26.7% vs 39%; aOR 0.21; 95% CI 0.09-0.46) as well as mixed feeding (46.5% vs 52.2%; aOR 0.28; 95% CI 0.13-0.60) at 1 week postpartum. No significant differences were observed in other aspects of labor process and birth outcomes, including the uptake of labor analgesia, postpartum hemorrhage (>500 mL) or neonatal outcomes. Conclusions: A positive maternal SARS-CoV-2 test in labor among women having vaginal birth was associated with a slightly longer duration of labor, increased likelihood of episiotomy, increased incidence of grade III meconium-stained amniotic fluid, a longer postpartum hospital stay and a lower rate of breastfeeding 1 week postpartum. However, it did not have an adverse impact on other birth outcomes.
MeSH term(s)	Pregnancy ; Infant, Newborn ; Female ; Humans ; Cohort Studies ; COVID-19/diagnosis ; COVID-19/epidemiology ; SARS-CoV-2 ; Labor, Obstetric ; Hospitalization ; Pregnancy Complications/epidemiology
Language	English
Publishing date	2023-11-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	80019-3
ISSN	1600-0412 ; 0001-6349
ISSN (online)	1600-0412
ISSN	0001-6349
DOI	10.1111/aogs.14704
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Article ; Online: Dynamic variations in and prediction of COVID-19 with omicron in the four first-tier cities of mainland China, Hong Kong, and Singapore

Xiaohua Ni / Bo Sun / Zengyun Hu / Qianqian Cui / Zhuo Zhang / Hua Zhang

Frontiers in Public Health, Vol

2023 Volume 11

Abstract: BackgroundThe COVID-19 pandemic, which began in late 2019, has resulted in the devastating collapse of the social economy and more than 10 million deaths worldwide. A recent study suggests that the pattern of COVID-19 cases will resemble a mini-wave ... ...

Abstract	BackgroundThe COVID-19 pandemic, which began in late 2019, has resulted in the devastating collapse of the social economy and more than 10 million deaths worldwide. A recent study suggests that the pattern of COVID-19 cases will resemble a mini-wave rather than a seasonal surge. In general, COVID-19 has more severe impacts on cities than on rural areas, especially in cities with high population density.MethodsIn this study, the background situation of COVID-19 transmission is discussed, including the population number and population density. Moreover, a widely used time series autoregressive integrated moving average (ARIMA) model is applied to simulate and forecast the COVID-19 variations in the six cities. We comprehensively analyze the dynamic variations in COVID-19 in the four first-tier cities of mainland China (BJ: Beijing, SH: Shanghai, GZ: Guangzhou and SZ: Shenzhen), Hong Kong (HK), China and Singapore (SG) from 2020 to 2022.ResultsThe major results show that the six cities have their own temporal characteristics, which are determined by the different control and prevention measures. The four first-tier cities of mainland China (i.e., BJ, SH, GZ, and SZ) have similar variations with one wave because of their identical “Dynamic COVID-19 Zero” strategy and strict Non-Pharmaceutical Interventions (NPIs). HK and SG have multiple waves primarily caused by the input cases. The ARIMA model has the ability to provide an accurate forecast of the COVID-19 pandemic trend for the six cities, which could provide a useful approach for predicting the short-term variations in infectious diseases.Accurate forecasting has significant value for implementing reasonable control and prevention measures.ConclusionsOur main conclusions show that control and prevention measures should be dynamically adjusted and organically integrated for the COVID-19 pandemic. Moreover, the mathematical models are proven again to provide an important scientific basis for disease control.
Keywords	COVID-19 ; omicron ; dynamic variations ; ARIMA ; prediction ; Public aspects of medicine ; RA1-1270
Subject code	720
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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