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  1. Article: Comparison of low-cost phantoms for ultrasound-guided fine-needle aspiration biopsy training.

    Zhao, Xiaofeng / Ersoy, Esma / Ng, Dianna L

    Journal of the American Society of Cytopathology

    2023  Volume 12, Issue 4, Page(s) 275–283

    Abstract: Introduction: Phantoms and simulators are widely accepted methods to gain valuable experience and confidence for inexperienced trainees prior to seeing their patient and for refining their skills. A phantom model that is durable, simple, and inexpensive ...

    Abstract Introduction: Phantoms and simulators are widely accepted methods to gain valuable experience and confidence for inexperienced trainees prior to seeing their patient and for refining their skills. A phantom model that is durable, simple, and inexpensive to produce and use would be ideal to train practitioners in ultrasound-guided fine-needle aspiration biopsy (USFNA) technique.
    Materials and methods: In this study, we systematically compared several low-cost phantom models including gelatin, extra firm tofu, canned cooked pork, ballistics gel, and chicken breast for their haptic properties, echogenicity, teaching utility, and overall performance based on a Likert scale (1-5; 5 = best). Nine cytopathologists and cytopathology fellows who perform FNA regularly evaluated these models and completed the survey.
    Results: The gelatin phantom, with a gelatin to water ratio of 1:8 by weight, was found to be the best for USFNA practice and overall performance, followed by the 1:10 gelatin phantom. Tofu and chicken breast phantoms were also good low-cost alternatives that needed only a few minutes of total preparation time.
    Conclusions: Low-cost, homemade phantoms can serve as excellent alternatives to commercial phantoms for practicing and teaching USFNA.
    MeSH term(s) Humans ; Biopsy, Fine-Needle/methods ; Gelatin ; Ultrasonography ; Image-Guided Biopsy ; Ultrasonography, Interventional
    Chemical Substances Gelatin (9000-70-8)
    Language English
    Publishing date 2023-03-24
    Publishing country United States
    Document type Journal Article
    ISSN 2213-2945
    ISSN 2213-2945
    DOI 10.1016/j.jasc.2023.03.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Neurotrophic receptor tyrosine kinase (NTRK) fusions and their role in cancer.

    Ruiz-Cordero, Roberto / Ng, Dianna L

    Cancer cytopathology

    2020  Volume 128, Issue 11, Page(s) 775–779

    MeSH term(s) Adult ; Child ; Cytodiagnosis/methods ; Female ; Humans ; Male ; Molecular Diagnostic Techniques/methods ; Neoplasms/genetics ; Neoplasms/metabolism ; Neoplasms/pathology ; Oncogene Proteins, Fusion ; Pathology, Molecular/standards ; Receptor Protein-Tyrosine Kinases/genetics
    Chemical Substances Oncogene Proteins, Fusion ; Receptor Protein-Tyrosine Kinases (EC 2.7.10.1)
    Language English
    Publishing date 2020-10-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2594979-2
    ISSN 1934-6638 ; 1934-662X
    ISSN (online) 1934-6638
    ISSN 1934-662X
    DOI 10.1002/cncy.22350
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Granulomatous inflammation diagnosed by fine-needle aspiration biopsy.

    Ng, Dianna L / Balassanian, Ronald

    Journal of the American Society of Cytopathology

    2019  Volume 8, Issue 6, Page(s) 317–323

    Abstract: Introduction: Fine-needle aspiration biopsy (FNAB) is a minimally invasive biopsy technique and an important tool for diagnosing infectious diseases. Rapid onsite evaluation allows for triage for ancillary testing, including microbiologic cultures. We ... ...

    Abstract Introduction: Fine-needle aspiration biopsy (FNAB) is a minimally invasive biopsy technique and an important tool for diagnosing infectious diseases. Rapid onsite evaluation allows for triage for ancillary testing, including microbiologic cultures. We aimed to determine the etiology of granulomatous inflammation diagnosed by FNAB by correlating with culture results and clinical history.
    Materials and methods: A 16-year retrospective review of cases diagnosed as "granulomatous inflammation" or "granuloma" was performed at the Departments of Pathology at the Zuckerberg San Francisco General Hospital and Trauma Center and University of California, San Francisco.
    Results: A total of 339 FNABs diagnosed as granulomatous inflammation were identified. Necrotizing granulomatous inflammation was present in 117 of 339 cases (34.5%) and non-necrotizing granulomatous inflammation was present in 222 of 339 cases (65.5%). A pathogen was detected in 100 of 339 (29.5%) FNABs by either cytomorphology, special stains, or culture, or a combination of more than one test. Of the 100 pathogen-positive cases, necrotizing granulomatous inflammation was seen in 50 of 100 (50%) and non-necrotizing granulomatous inflammation was identified in 50 of 100 (50%) cases. Culture results were available in 239 cases and positive in 70 (29%). Positive culture results included 40 of 239 (17%) cases with Mycobacterium tuberculosis complex, 15 of 239 (6.3%) with atypical mycobacterial species, 6 of 239 (3%) with Coccidioides immitis, 2 of 239 (<1%) with Histoplasma capsulatum, and 2 of 239 with Talaromyces marneffei (<1%).
    Conclusions: Granulomatous inflammation is a nonspecific finding and suggests a broad range of disease processes, ranging from infection to malignancy. FNAB is an excellent minimally invasive technique that allows for ancillary testing critical for definitive diagnosis.
    MeSH term(s) Adenocarcinoma/pathology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biopsy, Fine-Needle ; Child ; Child, Preschool ; Female ; Granuloma/microbiology ; Granuloma/pathology ; Humans ; Infant ; Inflammation/microbiology ; Inflammation/pathology ; Male ; Middle Aged ; Young Adult
    Language English
    Publishing date 2019-08-14
    Publishing country United States
    Document type Journal Article
    ISSN 2213-2945
    ISSN 2213-2945
    DOI 10.1016/j.jasc.2019.07.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Stop using expired plasma for cell blocks.

    Balassanian, Ronald / Ng, Dianna L / van Zante, Annemieke

    Cancer cytopathology

    2019  Volume 127, Issue 12, Page(s) 737–738

    MeSH term(s) DNA Contamination ; Humans ; Thrombin
    Chemical Substances Thrombin (EC 3.4.21.5)
    Language English
    Publishing date 2019-11-22
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 2594979-2
    ISSN 1934-6638 ; 1934-662X
    ISSN (online) 1934-6638
    ISSN 1934-662X
    DOI 10.1002/cncy.22204
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  5. Article ; Online: Evaluation of Women With a Positive Urine Cytology and no Demonstrable Disease in the Urinary Tract.

    Donat, Sherri M / Sonoda, Yukio / Al-Ahmadie, Hikmat / Murali, Rajmohan / Ng, Dianna L / Funt, Samuel A / Park, Kay J

    Urology

    2023  Volume 173, Page(s) 10–16

    Abstract: Urinary cytology is indispensable both for the evaluation of gross hematuria and surveillance of patients with urothelial neoplasms. A positive urine cytology usually indicates the presence of urothelial carcinoma somewhere in the urinary tract. However, ...

    Abstract Urinary cytology is indispensable both for the evaluation of gross hematuria and surveillance of patients with urothelial neoplasms. A positive urine cytology usually indicates the presence of urothelial carcinoma somewhere in the urinary tract. However, in women, it may also signal urothelial carcinoma involvement of the lower gynecologic tract or be the presenting sign for a primary cancer of the lower gynecologic tract or rectum. Guidelines for the evaluation of women with a positive cytology and normal urinary tract are lacking. We present a review of the current literature with case scenarios to bring clinicians attention to this diagnostic dilemma.
    MeSH term(s) Humans ; Female ; Carcinoma, Transitional Cell/diagnosis ; Carcinoma, Transitional Cell/pathology ; Urinary Bladder Neoplasms/pathology ; Cytology ; Urinary Tract/pathology ; Urologic Neoplasms/diagnosis ; Urine
    Language English
    Publishing date 2023-01-06
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 192062-5
    ISSN 1527-9995 ; 0090-4295
    ISSN (online) 1527-9995
    ISSN 0090-4295
    DOI 10.1016/j.urology.2022.12.032
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Infectious disease diagnosed by fine needle aspiration biopsy.

    Eslami, Arash / Can, Nhu Thuy / Ng, Dianna L

    Journal of the American Society of Cytopathology

    2020  Volume 9, Issue 3, Page(s) 152–158

    Abstract: Introduction: Fine needle aspiration biopsies (FNABs) have become increasingly important in the assessment of infectious diseases. We assess the ability of cytopathology to predict the presence of a pathogen and review how often neoplasia occurs ... ...

    Abstract Introduction: Fine needle aspiration biopsies (FNABs) have become increasingly important in the assessment of infectious diseases. We assess the ability of cytopathology to predict the presence of a pathogen and review how often neoplasia occurs concurrently with infection.
    Materials and methods: A 3-year retrospective review of FNABs with concurrent culture results was performed at the Zuckerberg San Francisco General Hospital and Trauma Center. Rapid onsite evaluation was performed for all cases by a pathologist. The results of the special and immunohistochemical stains and polymerase chain reaction testing were correlated, when available.
    Results: A total of 231 samples from 11 different tissue sites were submitted for microbial culture, of which 49 (21%) were positive for pathogenic organisms. Only 2 false-negative cases by cytology were found in immunosuppressed patients. A total of 38 patients had a diagnosis of neoplasia, with 2 (5%) having concurrent infection. Overall, the sensitivity and specificity of cytology in predicting the presence of infection was 96% (95% confidence interval, 86%-100%) and 42% (95% confidence interval, 34%-50%), respectively. Molecular testing was performed in 11 cases, 2 of which were positive for Mycobacterium tuberculosis complex and had cytologic findings of necrosis. Polymerase chain reaction and other ancillary tests were unable to further characterize 2 cases with acid-fast bacilli.
    Conclusions: Our study has shown that FNABs have high sensitivity in detecting infection and that negative cytology findings will correlate with a negative infectious workup. Although infection in the setting of neoplasia is uncommon, it should be considered if clinical data are available to suggest infection.
    MeSH term(s) Bacteria/isolation & purification ; Biopsy, Fine-Needle ; Communicable Diseases/complications ; Cytodiagnosis ; Humans ; Mycobacterium tuberculosis/isolation & purification ; Neoplasms/etiology ; Polymerase Chain Reaction ; Retrospective Studies ; Sensitivity and Specificity
    Language English
    Publishing date 2020-02-08
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2213-2945
    ISSN 2213-2945
    DOI 10.1016/j.jasc.2020.01.001
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  7. Article ; Online: Rapid Onsite Evaluation for Specimen Adequacy and Triage of Breast Masses in a Low-Resource Setting.

    Kimambo, Asteria H / Vuhahula, Edda A / Philipo, Godfrey S / Mushi, Beatrice P / Mmbaga, Elia J / Van Loon, Katherine / Ng, Dianna L

    Archives of pathology & laboratory medicine

    2023  Volume 148, Issue 1, Page(s) e9–e17

    Abstract: Context.—: Rapid onsite evaluation (ROSE) is critical in determining sample adequacy and triaging cytology samples. Although fine-needle aspiration biopsy (FNAB) is the primary method of initial tissue sampling in Tanzania, ROSE is not practiced.: ... ...

    Abstract Context.—: Rapid onsite evaluation (ROSE) is critical in determining sample adequacy and triaging cytology samples. Although fine-needle aspiration biopsy (FNAB) is the primary method of initial tissue sampling in Tanzania, ROSE is not practiced.
    Objective.—: To investigate the performance of ROSE in determining cellular adequacy and providing preliminary diagnoses in breast FNAB in a low-resource setting.
    Design.—: Patients with breast masses were recruited prospectively from the FNAB clinic at Muhimbili National Hospital. Each FNAB was evaluated by ROSE for overall specimen adequacy, cellularity, and preliminary diagnosis. The preliminary interpretation was compared to the final cytologic diagnosis and histologic diagnosis, when available.
    Results.—: Fifty FNAB cases were evaluated, and all were adequate for diagnosis on ROSE and final interpretation. Overall percentage of agreement (OPA) between preliminary and final cytologic diagnosis was 84%, positive percentage of agreement (PPA) was 33%, and negative percentage of agreement (NPA) was 100% (κ = 0.4, P < .001). Twenty-one cases had correlating surgical resections. OPA between preliminary cytologic and histologic diagnoses was 67%, PPA was 22%, and NPA was 100% (κ = 0.2, P = .09). OPA between final cytologic and histologic diagnoses was 95%, PPA was 89%, and NPA was 100% (κ = 0.9, P = <.001).
    Conclusions.—: False-positive rates of ROSE diagnoses for breast FNAB are low. While preliminary cytologic diagnoses had a high false-negative rate, final cytologic diagnoses had overall high concordance with histologic diagnoses. Therefore, the role of ROSE for preliminary diagnosis should be considered carefully in low-resource settings, and it may need to be paired with additional interventions to improve pathologic diagnosis.
    MeSH term(s) Humans ; Triage ; Breast ; Biopsy, Fine-Needle/methods ; Tanzania
    Language English
    Publishing date 2023-06-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 194119-7
    ISSN 1543-2165 ; 0363-0153 ; 0096-8528 ; 0003-9985
    ISSN (online) 1543-2165
    ISSN 0363-0153 ; 0096-8528 ; 0003-9985
    DOI 10.5858/arpa.2022-0398-OA
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  8. Article ; Online: A Large Thyroid Fine Needle Aspiration Biopsy Cohort with Long-Term Population-Based Follow-Up.

    Ng, Dianna L / van Zante, Annemieke / Griffin, Ann / Hills, Nancy K / Ljung, Britt-Marie

    Thyroid : official journal of the American Thyroid Association

    2021  Volume 31, Issue 7, Page(s) 1086–1095

    Abstract: Background: ...

    Abstract Background:
    MeSH term(s) Adenocarcinoma, Follicular/epidemiology ; Adenocarcinoma, Follicular/pathology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biopsy, Fine-Needle ; Child ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Retrospective Studies ; Thyroid Cancer, Papillary/epidemiology ; Thyroid Cancer, Papillary/pathology ; Thyroid Gland/pathology ; Thyroid Neoplasms/epidemiology ; Thyroid Neoplasms/pathology ; Thyroid Nodule/epidemiology ; Thyroid Nodule/pathology ; Young Adult
    Language English
    Publishing date 2021-01-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1086044-7
    ISSN 1557-9077 ; 1050-7256
    ISSN (online) 1557-9077
    ISSN 1050-7256
    DOI 10.1089/thy.2020.0689
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  9. Article ; Online: A roadmap to establishing global oncology as a priority initiative within a National Cancer Institute-designated cancer center.

    Van Loon, Katherine / Breithaupt, Lindsay / Ng, Dianna / DeBoer, Rebecca J / Buckle, Geoffrey C / Bialous, Stella / Hiatt, Robert A / Volberding, Paul / Hermiston, Michelle L / Ashworth, Alan

    Journal of the National Cancer Institute

    2023  Volume 116, Issue 3, Page(s) 345–351

    Abstract: As the burden of cancers impacting low- and middle-income countries is projected to increase, formation of strategic partnerships between institutions in high-income countries and low- and middle-income country institutions may serve to accelerate cancer ...

    Abstract As the burden of cancers impacting low- and middle-income countries is projected to increase, formation of strategic partnerships between institutions in high-income countries and low- and middle-income country institutions may serve to accelerate cancer research, clinical care, and training. As the US National Cancer Institute and its Center for Global Health continue to encourage cancer centers to join its global mission, academic cancer centers in the United States have increased their global activities. In 2015, the Helen Diller Family Comprehensive Cancer Center at the University of California, San Francisco, responded to the call for international partnership in addressing the global cancer burden through the establishment of the Global Cancer Program as a priority initiative. In developing the Global Cancer Program, we galvanized institutional support to foster sustained, bidirectional, equitable, international partnerships in global cancer control. Our focus and intent in disseminating this commentary is to share experiences and lessons learned from the perspective of a US-based, National Cancer Institute-designated cancer center and to provide a roadmap for other high-income institutions seeking to strategically broaden their missions and address the complex challenges of global cancer control. Herein, we review the formative evaluation, governance, strategic planning, investments in career development, funding sources, program evaluation, and lessons learned. Reflecting on the evolution of our program during the first 5 years, we observed in our partners a powerful shift toward a locally driven priority setting, reduced dependency, and an increased commitment to research as a path to improve cancer outcomes in resource-constrained settings.
    MeSH term(s) Humans ; United States/epidemiology ; Biomedical Research ; National Cancer Institute (U.S.) ; Medical Oncology ; Neoplasms/epidemiology ; Neoplasms/therapy ; Program Evaluation ; Global Health
    Language English
    Publishing date 2023-12-07
    Publishing country United States
    Document type Review ; Journal Article
    ZDB-ID 2992-0
    ISSN 1460-2105 ; 0027-8874 ; 0198-0157
    ISSN (online) 1460-2105
    ISSN 0027-8874 ; 0198-0157
    DOI 10.1093/jnci/djad255
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  10. Article ; Online: Feasibility of Fine-Needle Aspiration Biopsy and Rapid On-Site Evaluation for Immediate Triage in Breast Cancer Screening in Tanzania.

    Philipo, Godfrey S / Vuhahula, Edda / Kimambo, Asteria / Mmbaga, Elia J / Van Loon, Katherine / Ng, Dianna L

    JCO global oncology

    2021  Volume 7, Page(s) 146–152

    Abstract: Purpose: Clinical breast examination (CBE) is one of the most common methods used for early detection of breast cancer in low- and middle-income countries. CBE alone is limited by lack of specificity and may result in unnecessary diagnostic procedures. ... ...

    Abstract Purpose: Clinical breast examination (CBE) is one of the most common methods used for early detection of breast cancer in low- and middle-income countries. CBE alone is limited by lack of specificity and may result in unnecessary diagnostic procedures. We evaluated the feasibility of integrating CBE, fine-needle aspiration biopsy (FNAB), and rapid on-site evaluation (ROSE) in triaging palpable breast masses for specialized cancer care.
    Materials and methods: An intensive breast cancer screening event was conducted at a national trade fair by a multidisciplinary team of care providers targeting a healthy population in Dar es Salaam, Tanzania. All adults age ≥ 18 years were invited to participate. CBE was performed by oncologists and/or pathologists. FNAB was performed by a pathologist on palpable masses that were then categorized as benign, indeterminate, or suspicious for malignancy or definitively malignant based on ROSE.
    Results: A total of 208 individuals (207 females, one male; median age, 36 years; range, 18-68 years) were screened. Most (90.8%, 189 of 208) had normal findings, whereas 7.2% (15 of 208), 1% (2 of 208), and 1% (2 of 208) had a palpable mass, breast pain, and nipple discharge, respectively. Two participants had lesions too small for palpation-guided biopsy and clinically consistent with fibroadenomas; the participants were counseled, and observation was recommended. FNAB was performed on 13 breast masses, with 9 of 13 (69%) categorized as benign and 4 of 13 (31%) suspicious for malignancy. Final cytopathologic review of referred patients confirmed one case to be breast adenocarcinoma, one was suggestive of fibroadenoma, and two showed inflammations.
    Conclusion: Integration of CBE with ROSE and FNAB was feasible in a breast cancer screening program in Tanzania. In settings with constrained resources for cancer care, this may be an effective method for triaging patients with breast masses.
    MeSH term(s) Adolescent ; Adult ; Aged ; Biopsy, Fine-Needle ; Breast Neoplasms/diagnosis ; Early Detection of Cancer ; Feasibility Studies ; Female ; Humans ; Male ; Middle Aged ; Tanzania ; Triage ; Young Adult
    Language English
    Publishing date 2021-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 2687-8941
    ISSN (online) 2687-8941
    DOI 10.1200/GO.20.00279
    Database MEDical Literature Analysis and Retrieval System OnLINE

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