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Article ; Online: Recombinant production and characterization of L-glutaminase (glsA) as a promiscuity therapeutic enzyme.

Simay, Shayan / Akbarzadeh-Khiavi, Mostafa / Pourseif, Mohammad M / Barar, Jaleh / Safary, Azam / Omidi, Yadollah

2022 Volume 106, Issue 17, Page(s) 5511–5524

Abstract: Because of the therapeutical impacts of hydrolytic enzymes in different diseases, in particular malignancies, we aimed to produce a recombinant putative L-glutaminase (GLS ... ...

Abstract	Because of the therapeutical impacts of hydrolytic enzymes in different diseases, in particular malignancies, we aimed to produce a recombinant putative L-glutaminase (GLS A
MeSH term(s)	Asparaginase ; Asparagine ; Bacillus ; Glutaminase ; Glutamine ; Humans ; Molecular Docking Simulation ; Neoplasms
Chemical Substances	Glutamine (0RH81L854J) ; Asparagine (7006-34-0) ; Asparaginase (EC 3.5.1.1) ; Glutaminase (EC 3.5.1.2)
Language	English
Publishing date	2022-07-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-022-12058-y
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Article: A multi-method and structure-based in silico vaccine designing against

Pourseif, Mohammad Mostafa / Yousefpour, Mitra / Aminianfar, Mohammad / Moghaddam, Gholamali / Nematollahi, Ahmad

BioImpacts : BI

2019 Volume 9, Issue 3, Page(s) 131–144

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2019-03-08
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2604624-6
ISSN	2228-5660 ; 2228-5652
ISSN (online)	2228-5660
ISSN	2228-5652
DOI	10.15171/bi.2019.18
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Article: A domain-based vaccine construct against SARS-CoV-2, the causative agent of COVID-19 pandemic: development of self-amplifying mRNA and peptide vaccines.

Pourseif, Mohammad Mostafa / Parvizpour, Sepideh / Jafari, Behzad / Dehghani, Jaber / Naghili, Behrouz / Omidi, Yadollah

BioImpacts : BI

2020 Volume 11, Issue 1, Page(s) 65–84

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2020-12-10
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2604624-6
ISSN	2228-5660 ; 2228-5652
ISSN (online)	2228-5660
ISSN	2228-5652
DOI	10.34172/bi.2021.11
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Article: Recombinant production and characterization of L-glutaminase (glsA) as a promiscuity therapeutic enzyme

Simay, Shayan / Akbarzadeh-Khiavi, Mostafa / Pourseif, Mohammad M. / Barar, Jaleh / Safary, Azam / Omidi, Yadollah

Applied microbiology and biotechnology. 2022 Sept., v. 106, no. 17

2022

Abstract: Because of the therapeutical impacts of hydrolytic enzymes in different diseases, in particular malignancies, we aimed to produce a recombinant putative L-glutaminase (GLS ASL₋₁) from a recently characterized halo-thermotolerant Bacillus sp. SL-1. For ... ...

Abstract	Because of the therapeutical impacts of hydrolytic enzymes in different diseases, in particular malignancies, we aimed to produce a recombinant putative L-glutaminase (GLS ASL₋₁) from a recently characterized halo-thermotolerant Bacillus sp. SL-1. For this purpose, the glsA gene was identified and efficiently overexpressed in the Origami™ B (DE3) strain. The yield of the purified GLS ASL₋₁ was ~ 20 mg/L, indicating a significant expression of recombinant enzyme in the Origami. The enzyme activity assay revealed a significant hydrolytic effect of the recombinant GLS ASL₋₁ on L-asparagine (Asn) (i.e., Kₘ 39.8 μM, kcₐₜ 19.9 S⁻¹) with a minimal affinity for L-glutamine (Gln). The GLS ASL₋₁ significantly suppressed the growth of leukemic Jurkat cells through apoptosis induction (47.5%) in the IC₅₀ dosage of the enzyme. The GLS ASL₋₁ could also change the Bax/Bcl2 expression ratio, indicating its apoptotic effect on cancer cells. The in silico analysis was conducted to predict structural features related to the histidine-tag exposure in the N- or C-terminal of the recombinant GLS ASL₋₁. In addition, molecular docking simulation for substrate specificity revealed a greater binding affinity of Asn to the enzyme binding-site residues than Gln, which was confirmed in experimental procedures as well. In conclusion, the current study introduced a recombinant GLS ASL₋₁ with unique functional and structural features, highlighting its potential pharmaceutical and medical importance. GLS ASL₋₁ represents the first annotated enzyme from Bacillus with prominent asparaginase activity, which can be considered for developing alternative enzymes in therapeutic applications. KEY POINTS: • Hydrolytic enzymes have critical applications in different types of human malignancies. • A recombinant L-glutaminase (GLS ASL₋₁) was produced from halo-thermotolerant Bacillus sp. SL-1. • GLS ASL₋₁ displayed a marked hydrolytic activity on L-asparagine compared to the L-glutamine. • GLS ASL₋₁ with significant substrate promiscuity may be an alternative for developing novel pharmaceuticals.
Keywords	Bacillus (bacteria) ; apoptosis ; asparaginase ; asparagine ; biotechnology ; computer simulation ; drugs ; enzyme activity ; genes ; glutaminase ; glutamine ; humans ; microbiology ; substrate specificity ; therapeutics
Language	English
Dates of publication	2022-09
Size	p. 5511-5524.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-022-12058-y
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Designing a Polytopic Protective Antigen Against Echinococcus granulosus for the Both of Definitive and Intermediate Hosts

• Gholamali Moghaddam, Mohammad Mostafa Pourseif, Hossein Daghighkia, Ahmad Nematollahi

پژوهش های علوم دامی, Vol 31, Iss 2, Pp 15-

2021 Volume 27

Abstract: ... Pourseif et al. 2017a). Altogether, ... ...

Abstract	Introduction: The hydatid disease is one of the most important zoonotic parasitic infections all-round the world. The main part of Echinococcus granulosus life cycle depends upon the growth and differentiation of protoscoleces (PSCs) within the intestine of definitive hosts (Carmena and Cardona 2014). The survival of organism is mainly dependent on its indirect transmission cycle from the definitive hosts (particularly dogs) to the intermediate hosts, including sheep and human. According to the latest updated report of the World Health Organization (WHO) in March 2017, there may be over one million infected cases with echinococcosis at any time (WHO, 2017). A hydatid cyst (HC) encompasses numerous PSCs and cyst fluid, and is formed in visceral organs (liver and lung) of the infected intermediate hosts (Bingham et al. 2014). Morphogenesis of PSCs from the cystic viscera into worm’s head occurs on the surface of the small intestine of the definitive host, and then the head-like structure attaches to the gut epithelial lining and develops into an adult worm within ~50 days (Moro and Schantz 2009). The currently used treatment modalities against echinococcosis, as multi-stage parasitic infection, are based on the anthelmintic drugs such as praziquantel. In this case there is an emergency to improve preventive interventions such as vaccination in addition to hygiene practices. Several constraining factors may influence the vaccine development against such multi-stage pathogens, including economic, socio-cultural issues (Bethony et al. 2011). Thus, it is necessary to implement a rationalized approach towards construction of multipotent vaccines. In silico modeling of vaccines provides a cost- and time-effective approach that can improve such developing effective vaccines (Gori et al. 2013). Ideally, a vaccine construct, to be highly effective, should encompass several parts, including epitopes of one/more VCAs, B-cells epitope and T-cells epitopes (BEs and TEs, respectively) (Pourseif et al. 2017a). Altogether, ...
Keywords	cellular immunity ; echinococcus granulosus ; epitope ; humoral immunity ; Animal biochemistry ; QP501-801 ; Dairy processing. Dairy products ; SF250.5-275 ; Veterinary medicine ; SF600-1100 ; Dairying ; SF221-250
Subject code	630
Language	English
Publishing date	2021-09-01T00:00:00Z
Publisher	University of tabriz
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A multi-method and structure-based in silico vaccine designing against Echinococcus granulosus through investigating enolase protein

Mohammad Mostafa Pourseif / Mitra Yousefpour / Mohammad Aminianfar / Gholamali Moghaddam / Ahmad Nematollahi

BioImpacts, Vol 9, Iss 3, Pp 131-

2019 Volume 144

Abstract: Introduction: Hydatid disease is a ubiquitous parasitic zoonotic disease, which causes different medical, economic and serious public health problems in some parts of the world. The causal organism is a multi-stage parasite named Echinococcus granulosus ... ...

Abstract	Introduction: Hydatid disease is a ubiquitous parasitic zoonotic disease, which causes different medical, economic and serious public health problems in some parts of the world. The causal organism is a multi-stage parasite named Echinococcus granulosus whose life cycle is dependent on two types of mammalian hosts viz definitive and intermediate hosts. Methods: In this study, enolase, as a key functional enzyme in the metabolism of E. granulosus (EgEnolase), was targeted through a comprehensive in silico modeling analysis and designing a host-specific multi-epitope vaccine. Three-dimensional (3D) structure of enolase was modeled using MODELLER v9.18 software. The B-cell epitopes (BEs) were predicted based on the multi-method approach and via some authentic online predictors. ClusPro v2.0 server was used for docking-based T-helper epitope prediction. The 3D structure of the vaccine was modeled using the RaptorX server. The designed vaccine was evaluated for its immunogenicity, physicochemical properties, and allergenicity. The codon optimization of the vaccine sequence was performed based on the codon usage table of E. coli K12. Finally, the energy minimization and molecular docking were implemented for simulating the vaccine binding affinity to the TLR-2 and TLR-4 and the complex stability. Results: The designed multi-epitope vaccine was found to induce anti-EgEnolase immunity which may have the potential to prevent the survival and proliferation of E. granulosus into the definitive host. Conclusion: Based on the results, this step-by-step immunoinformatics approach could be considered as a rational platform for designing vaccines against such multi-stage parasites. Furthermore, it is proposed that this multi-epitope vaccine is served as a promising preventive anti-echinococcosis agent.
Keywords	echinococcus granulosus ; enolase ; in silico vaccinology ; molecular docking ; epitope ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2019-04-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Current status and future prospective of vaccine development against Echinococcus granulosus

Pourseif, Mohammad Mostafa / Gholamali Moghaddam / Nazli Saeedi / Abolfazl Barzegari / Jaber Dehghani / Yadollah Omidi

Biologicals. 2017,

2017

Abstract: Cystic echinococcosis (CE) is one of the most important zoonotic parasite diseases in human, livestock and wildlife worldwide. Development of effective vaccines against CE appears to be the most promising strategy to control this infectious disease. Use ... ...

Abstract	Cystic echinococcosis (CE) is one of the most important zoonotic parasite diseases in human, livestock and wildlife worldwide. Development of effective vaccines against CE appears to be the most promising strategy to control this infectious disease. Use of potential livestock and canine vaccines against larval and adult stage of E. granulosus life cycle may be the key for the production of powerful vaccines. Some progress has been accomplished on development of vaccines against hydatidosis using empirical approaches, while such immunotherapies often fail to induce adequate immune responses. Therefore, it is of great interest to identify antigens (Ags) with high immunogenicity and develop effective vaccines and adjuvant constructs against CE. To this end, various tools can be applied, including immune-based genomics and proteomics, immunoinformatics, systems vaccinology and mathematical/computational modeling. In this review, we aimed to provide comprehensive insights upon current status of vaccination trials against E. granulosus, and also articulate some perspectives on production of novel anti-CE vaccines. Use of novel prospective technologies is also discussed to highlight the importance of development and advancement of the next generation vaccines against E. granulosus.
Keywords	Echinococcus granulosus ; adjuvants ; adults ; antigens ; dogs ; echinococcosis ; genomics ; humans ; immune response ; immunoinformatics ; infectious diseases ; larvae ; livestock ; models ; parasites ; proteomics ; vaccination ; vaccine development ; vaccines ; wildlife
Language	English
Size	p. .
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	1017370-5
ISSN	1095-8320 ; 1045-1056
ISSN (online)	1095-8320
ISSN	1045-1056
DOI	10.1016/j.biologicals.2017.10.003
Database	NAL-Catalogue (AGRICOLA)

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Article: Modulatory Role of Vaginal-Isolated Lactococcus lactis on the Expression of miR-21, miR-200b, and TLR-4 in CAOV-4 Cells and In Silico Revalidation

Rahbar Saadat, Yalda / Pourseif, Mohammad Mostafa / Zununi Vahed, Sepideh / Barzegari, Abolfazl / Omidi, Yadollah / Barar, Jaleh

Probiotics and antimicrobial proteins. 2020 Sept., v. 12, no. 3

2020

Abstract: Ovarian cancer (OC) is a leading cause of death among women worldwide. Various evidences suggest that oncomiRs and Toll-like receptor 4 (TLR-4) signaling pathways appear to be key players in the initiation and progression of OC. It seems there exists a ... ...

Abstract	Ovarian cancer (OC) is a leading cause of death among women worldwide. Various evidences suggest that oncomiRs and Toll-like receptor 4 (TLR-4) signaling pathways appear to be key players in the initiation and progression of OC. It seems there exists a continuous intercommunication between cancer cells and normal microbiota of the vagina. The biological impacts of vaginal isolated lactococcus lactis on CAOV-4 cells were investigated using several molecular biology experiments, including flow cytometry, DAPI staining, DNA ladder, and scratch assay. The expression of microRNAs (miRNAs/miRs) 21, 200b, and TLR-4 in the CAOV-4 cells was also evaluated by the real-time RT-PCR assay. Furthermore, an integrative in silico analysis was conducted using normalized web-available microarray data (GSE14407) to revalidate the experimental findings and identify potential biomarkers in ovarian cancer. Protein-protein interactions (PPIs) network was studied by means of the STRING database using Cytoscape v3.6.1. The miRNA target genes were identified using the dbDEMC v2.0, miRTarBase, and miRDB databases. Our data demonstrated that L. lactis probiotic candidate downregulates TLR-4, miR-21, and miR-200b expression levels, which correlates with induction of apoptosis as confirmed by DAPI staining, DNA ladder assay, annexin V/PI staining, and inhibition of migration validated by scratch assay. By in silico analysis, several targets (miR-17-5p-BCL2, miR-21-5p-MKNK2, miR-129-5p-CDK6) were identified, while BCL2, CCNB1, and VEGFA were found as the hub proteins in the miRNA-target and PPI networks. Further, downregulation of the TLR-4, miR-21, and miR-200b was partially validated by the in silico analysis. Based on our findings, the vaginal isolated probiotic strain presents great potential to control the ovarian cancer which may provide beneficial impact on the clinical management of ovarian cancer.
Keywords	4',6-diamidino-2-phenylindole ; DNA ; Lactococcus lactis ; Toll-like receptor 4 ; animal ovaries ; apoptosis ; biomarkers ; computer simulation ; databases ; flow cytometry ; gene expression regulation ; genes ; microRNA ; microarray technology ; molecular biology ; neoplasm cells ; ovarian neoplasms ; probiotics ; protein-protein interactions ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; signal transduction ; staining ; vagina ; women
Language	English
Dates of publication	2020-09
Size	p. 1083-1096.
Publishing place	Springer US
Document type	Article
ZDB-ID	2487792-X
ISSN	1867-1314 ; 1867-1306
ISSN (online)	1867-1314
ISSN	1867-1306
DOI	10.1007/s12602-019-09596-9
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Modulatory Role of Vaginal-Isolated Lactococcus lactis on the Expression of miR-21, miR-200b, and TLR-4 in CAOV-4 Cells and In Silico Revalidation.

Rahbar Saadat, Yalda / Pourseif, Mohammad Mostafa / Zununi Vahed, Sepideh / Barzegari, Abolfazl / Omidi, Yadollah / Barar, Jaleh

Probiotics and antimicrobial proteins

2019 Volume 12, Issue 3, Page(s) 1083–1096

Abstract	Ovarian cancer (OC) is a leading cause of death among women worldwide. Various evidences suggest that oncomiRs and Toll-like receptor 4 (TLR-4) signaling pathways appear to be key players in the initiation and progression of OC. It seems there exists a continuous intercommunication between cancer cells and normal microbiota of the vagina. The biological impacts of vaginal isolated lactococcus lactis on CAOV-4 cells were investigated using several molecular biology experiments, including flow cytometry, DAPI staining, DNA ladder, and scratch assay. The expression of microRNAs (miRNAs/miRs) 21, 200b, and TLR-4 in the CAOV-4 cells was also evaluated by the real-time RT-PCR assay. Furthermore, an integrative in silico analysis was conducted using normalized web-available microarray data (GSE14407) to revalidate the experimental findings and identify potential biomarkers in ovarian cancer. Protein-protein interactions (PPIs) network was studied by means of the STRING database using Cytoscape v3.6.1. The miRNA target genes were identified using the dbDEMC v2.0, miRTarBase, and miRDB databases. Our data demonstrated that L. lactis probiotic candidate downregulates TLR-4, miR-21, and miR-200b expression levels, which correlates with induction of apoptosis as confirmed by DAPI staining, DNA ladder assay, annexin V/PI staining, and inhibition of migration validated by scratch assay. By in silico analysis, several targets (miR-17-5p-BCL2, miR-21-5p-MKNK2, miR-129-5p-CDK6) were identified, while BCL2, CCNB1, and VEGFA were found as the hub proteins in the miRNA-target and PPI networks. Further, downregulation of the TLR-4, miR-21, and miR-200b was partially validated by the in silico analysis. Based on our findings, the vaginal isolated probiotic strain presents great potential to control the ovarian cancer which may provide beneficial impact on the clinical management of ovarian cancer.
MeSH term(s)	Apoptosis ; Cell Line, Tumor ; Female ; Gene Expression ; Humans ; Lactococcus lactis ; MicroRNAs/metabolism ; Probiotics ; Toll-Like Receptor 4/metabolism ; Vagina/metabolism ; Vagina/microbiology
Chemical Substances	MIRN200 microRNA, human ; MIRN21 microRNA, human ; MicroRNAs ; TLR4 protein, human ; Toll-Like Receptor 4
Language	English
Publishing date	2019-12-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2487792-X
ISSN	1867-1314 ; 1867-1306
ISSN (online)	1867-1314
ISSN	1867-1306
DOI	10.1007/s12602-019-09596-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Current status and future prospective of vaccine development against Echinococcus granulosus.

Pourseif, Mohammad Mostafa / Moghaddam, Gholamali / Saeedi, Nazli / Barzegari, Abolfazl / Dehghani, Jaber / Omidi, Yadollah

Biologicals : journal of the International Association of Biological Standardization

2017 Volume 51, Page(s) 1–11

Abstract: Cystic echinococcosis (CE) is one of the most important zoonotic parasite diseases in human, livestock, and wildlife worldwide. Development of effective vaccines against CE appears to be the most promising strategy to control this infectious disease. Use ...

Abstract	Cystic echinococcosis (CE) is one of the most important zoonotic parasite diseases in human, livestock, and wildlife worldwide. Development of effective vaccines against CE appears to be the most promising strategy to control this infectious disease. Use of potential livestock and canine vaccines against the larval and adult stage of E. granulosus life cycle may be the key to the production of powerful vaccines. Some progress has been accomplished in the development of vaccines against hydatidosis using empirical approaches, while such immunotherapies often fail to induce adequate immune responses. Therefore, it is of great interest to identify antigens (Ags) with high immunogenicity and develop effective vaccines and adjuvant constructs against CE. To this end, various tools can be applied, including immune-based genomics and proteomics, immunoinformatics, systems vaccinology and mathematical/computational modeling. In this review, we aimed to provide comprehensive insights upon the current status of vaccination trials against E. granulosus, and also articulate some perspectives on the production of novel anti-CE vaccines. Use of novel prospective technologies is also discussed to highlight the importance of development and advancement of the next generation vaccines against E. granulosus.
MeSH term(s)	Animals ; Echinococcosis/immunology ; Echinococcosis/parasitology ; Echinococcosis/prevention & control ; Echinococcus granulosus/immunology ; Host-Parasite Interactions/immunology ; Humans ; Prospective Studies ; Vaccines/administration & dosage ; Vaccines/immunology ; Zoonoses/immunology ; Zoonoses/parasitology
Chemical Substances	Vaccines
Language	English
Publishing date	2017-11-03
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1017370-5
ISSN	1095-8320 ; 1045-1056
ISSN (online)	1095-8320
ISSN	1045-1056
DOI	10.1016/j.biologicals.2017.10.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

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ab Jg. 2022: Lesesaal (EG)

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