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  1. Article ; Online: Expectations for employing

    Hu, Miaomiao / Zhang, Tao / Miao, Ming / Li, Kewen / Luan, Qingmin / Sun, Guilian

    Critical reviews in food science and nutrition

    2024  , Page(s) 1–9

    Abstract: As a promising probiotic strain, ...

    Abstract As a promising probiotic strain,
    Language English
    Publishing date 2024-01-08
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1037504-1
    ISSN 1549-7852 ; 1040-8398
    ISSN (online) 1549-7852
    ISSN 1040-8398
    DOI 10.1080/10408398.2023.2301416
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1459: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Transcriptome Analysis Reveals Candidate Genes Involved in Gibberellin-Induced Fruit Development in

    Huang, Xiaolong / Wu, Xiaoai / Sun, Guilian / Jiang, Yu / Yan, Huiqing

    Plants (Basel, Switzerland)

    2023  Volume 12, Issue 19

    Abstract: Gibberellins (GAs) play indispensable roles in the fruit development of horticultural plants. Unfortunately, the molecular basis behind GAs regulating fruit development ... ...

    Abstract Gibberellins (GAs) play indispensable roles in the fruit development of horticultural plants. Unfortunately, the molecular basis behind GAs regulating fruit development in
    Language English
    Publishing date 2023-09-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants12193425
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  3. Article ; Online: Transcriptome Analysis Reveals Regulatory Networks and Hub Genes in the Flavonoid Metabolism of Rosa roxburghii

    Huang, Xiaolong / Sun, Guilian / Li, Qiaohong / Yan, Huiqing

    Horticulturae. 2023 Feb. 09, v. 9, no. 2

    2023  

    Abstract: Rosa roxburghii Tratt, the most popular fruit that blooms in the southwest of China, has high antioxidant properties and is rich in different flavonoids. However, the regulatory network and critical genes that regulate the flavonoid biosynthesis of R. ... ...

    Abstract Rosa roxburghii Tratt, the most popular fruit that blooms in the southwest of China, has high antioxidant properties and is rich in different flavonoids. However, the regulatory network and critical genes that regulate the flavonoid biosynthesis of R. roxburghii are still unknown. In this study, HPLC analysis revealed that total flavonoids, anthocyanins, and catechin were enriched in mature fruits, flowers, and leaves, respectively. Differentially expressed genes (DEGs) between five organs of R. roxburghii involved in flavonoid metabolism were obtained by transcriptome sequencing. A total of 1130 DEGs were identified, including 166 flavonoid pathway biosynthesis genes, 622 transcription factors (TFs), 301 transporters, and 221 cytochrome P450 proteins. A weighted gene co-expression network analysis (WGCNA) of the DEGs was conducted to construct co-expression networks. Regarding enzymes in the biosynthesis of flavonoids, cytochrome P450 CYP749A22 and CYP72A219 were highlighted in the regulation of total flavonoids of mature fruits. Anthocyanin 3-O-glucosyltransferase and F3′H were the top two critical enzymes for anthocyanin accumulation in flowers. By contrast, caffeic acid 3-O-methyltransferase, 4-coumarate-CoA ligase, and shikimate O-hydroxycinnamoyltransferase were essential for catechin accumulation in leaves. Additionally, we analyzed the eigengene network of the “black” module, which had high correlations with total flavonoids (r = 0.9, p = 5 × 10⁻⁶). There were 26 eigengenes in the “black” module, consisting of 6 flavonoid biosynthesis, 14 TFs, and 6 transporters. Among them, the transcription factors RrWRKY45 (DN142829_c1_g5), RrTCP20 (DN146443_c1_g1), and RrERF118 (DN141507_c3_g2) were screened as the hub genes, which significantly correlated with total flavonoids in R. roxburghii. The present biochemical and transcriptomic data provide insights into functional genomics for breeding R. roxburghii with flavonoid accumulation.
    Keywords Rosa roxburghii ; anthocyanins ; antioxidants ; biosynthesis ; caffeate O-methyltransferase ; catechin ; coumarate-CoA ligase ; cytochrome P-450 ; fruits ; gene expression regulation ; genes ; genomics ; hydroxycinnamoyltransferase ; transcriptome ; transcriptomics ; China
    Language English
    Dates of publication 2023-0209
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2813983-5
    ISSN 2311-7524
    ISSN 2311-7524
    DOI 10.3390/horticulturae9020233
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Transcriptomic Profiling of Cold Stress-Induced Differentially Expressed Genes in Seedling Stage of

    Yan, Tao / Sun, Meng / Su, Rui / Wang, Xiaozhong / Lu, Xuedan / Xiao, Yunhua / Deng, Huabing / Liu, Xiong / Tang, Wenbang / Zhang, Guilian

    Plants (Basel, Switzerland)

    2023  Volume 12, Issue 14

    Abstract: Cold stress significantly constrains the growth, development, productivity, and distribution of rice, particularly ... ...

    Abstract Cold stress significantly constrains the growth, development, productivity, and distribution of rice, particularly the
    Language English
    Publishing date 2023-07-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants12142675
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  5. Article: Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry.

    Yan, Huiqing / Ma, Dandan / Yi, Peipei / Sun, Guilian / Chen, Xingyan / Yi, Yin / Huang, Xiaolong

    Plant methods

    2023  Volume 19, Issue 1, Page(s) 99

    Abstract: Background: The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family. Agrobacterium rhizogenes-mediated hairy root ... ...

    Abstract Background: The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family. Agrobacterium rhizogenes-mediated hairy root transformation is an alternative for exploring gene functions, especially the genes specifically expressed in roots. However, the hairy root transformation has not been established in strawberry.
    Results: Here, we described an efficient and rapid hairy root transgenic system for strawberry using A. rhizogenes. Strain of A. rhizogenes MSU440 or C58C1 was the most suitable for hairy root transformation. The transformation efficiency was highest when tissues contained hypocotyls as explants. The optimal procedure involves A. rhizogenes at an optical density (OD
    Conclusion: We established a rapid and efficient hairy root transformation in strawberry by optimizing parameters, which was adequate for promoter analysis and functional characterization of candidate genes in strawberry and other rosaceous plants.
    Language English
    Publishing date 2023-09-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2203723-8
    ISSN 1746-4811
    ISSN 1746-4811
    DOI 10.1186/s13007-023-01078-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Human milk oligosaccharide lacto-N-tetraose: Physiological functions and synthesis methods.

    Hu, Miaomiao / Miao, Ming / Li, Kewen / Luan, Qingmin / Sun, Guilian / Zhang, Tao

    Carbohydrate polymers

    2023  Volume 316, Page(s) 121067

    Abstract: Human milk oligosaccharides (HMOs) have attracted considerable attention due to their unique role in boosting infant health. Among the HMOs, lacto-N-tetraose (LNT) is a significant constituent associated with various health benefits, such as prebiotic ... ...

    Abstract Human milk oligosaccharides (HMOs) have attracted considerable attention due to their unique role in boosting infant health. Among the HMOs, lacto-N-tetraose (LNT) is a significant constituent associated with various health benefits, such as prebiotic effects, antiadhesive antimicrobials, antiviral protection, and immune modulators. LNT has received a "Generally Recognized as Safe" status by the American Food and Drug Administration and was approved as a food ingredient for infant formula. However, the limited availability of LNT poses a major challenge for its application in food and medicine. In this review, we first explored the physiological functions of LNT. Next, we describe several synthesis methods for production of LNT, including chemical, enzymatic, and cell factory approaches, and summarize the pivotal research results. Finally, challenges and opportunities for the large-scale synthesis of LNT were discussed.
    MeSH term(s) Infant ; Humans ; Milk, Human ; Oligosaccharides ; Infant Formula
    Chemical Substances lacto-N-neotetraose (BY63N40B1L) ; Oligosaccharides
    Language English
    Publishing date 2023-05-25
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2023.121067
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Integrated design and optimization of reactor-distillation sequence-recycle-heat exchanger network

    Yin, Changfang / Sun, Huifeng / Lv, Donghui / Liu, Guilian

    Energy. 2022 Jan. 01, v. 238

    2022  

    Abstract: In the chemical process, reactor and distillation sequence are interconnected by the reactor effluent and recycle streams, and affect the heat exchanger network (HEN). A systematic method is proposed for synthesizing and optimizing the Reactor- ... ...

    Abstract In the chemical process, reactor and distillation sequence are interconnected by the reactor effluent and recycle streams, and affect the heat exchanger network (HEN). A systematic method is proposed for synthesizing and optimizing the Reactor-Distillation sequence-Recycle-HEN (RDRH) system. In this method, an improved partition method is used to identify all feasible distillation sequences; each distillation sequence is integrated with the reactor, the recycle and HEN. This method can identify the RDRH system with the minimum energy consumption and the corresponding key parameters efficiently. A styrene unit is studied by the proposed method, the results are in good agreement with those identified based on the rigorous simulation of Aspen Plus, and the relative errors are less than 5.5%. The proposed method can be applied in the preliminary design for targeting the optimal flowsheet and parameters, comparing catalysts, and can be extended to optimize the flowsheet in the whole life cycle.
    Keywords distillation ; energy ; heat exchangers ; styrene
    Language English
    Dates of publication 2022-0101
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2019804-8
    ISSN 0360-5442 ; 0360-5442
    ISSN (online) 0360-5442
    ISSN 0360-5442
    DOI 10.1016/j.energy.2021.121796
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger.

    Wan, Xiufen / Wang, Lu / Chang, Jingjing / Zhang, Jing / Zhang, Zhiyun / Li, Kewen / Sun, Guilian / Liu, Caixia / Zhong, Yaohua

    Microbial cell factories

    2024  Volume 23, Issue 1, Page(s) 76

    Abstract: Background: Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS ... ...

    Abstract Background: Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS accompanied by the by-product (glucose) generation. This study aims to consume glucose to enhance the content of FOS by heterologously expressing glucose oxidase and peroxidase in engineered A. niger.
    Results: Glucose oxidase was successfully expressed and co-localized with β-fructofuranosidase in mycelia. These mycelia were applied to synthesis of FOS, which possessed an increased purity of 60.63% from 52.07%. Furthermore, peroxidase was expressed in A. niger and reached 7.70 U/g, which could remove the potential inhibitor of glucose oxidase to facilitate the FOS synthesis. Finally, the glucose oxidase-expressing strain and the peroxidase-expressing strain were jointly used to synthesize FOS, which content achieved 71.00%.
    Conclusions: This strategy allows for obtaining high-content FOS by the multiple enzymes expressed in the industrial fungus, avoiding additional purification processes used in the production of oligosaccharides. This study not only facilitated the high-purity FOS synthesis, but also demonstrated the potential of A. niger ATCC 20611 as an enzyme-producing cell factory.
    MeSH term(s) Aspergillus niger/genetics ; beta-Fructofuranosidase ; Glucose Oxidase/genetics ; Oligosaccharides ; Peroxidases ; Glucose ; Aspergillus
    Chemical Substances fructooligosaccharide ; beta-Fructofuranosidase (EC 3.2.1.26) ; Glucose Oxidase (EC 1.1.3.4) ; Oligosaccharides ; Peroxidases (EC 1.11.1.-) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2024-03-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-024-02353-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

    Huiqing Yan / Dandan Ma / Peipei Yi / Guilian Sun / Xingyan Chen / Yin Yi / Xiaolong Huang

    Plant Methods, Vol 19, Iss 1, Pp 1-

    2023  Volume 12

    Abstract: Abstract Background The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family. Agrobacterium rhizogenes-mediated hairy root ... ...

    Abstract Abstract Background The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family. Agrobacterium rhizogenes-mediated hairy root transformation is an alternative for exploring gene functions, especially the genes specifically expressed in roots. However, the hairy root transformation has not been established in strawberry. Results Here, we described an efficient and rapid hairy root transgenic system for strawberry using A. rhizogenes. Strain of A. rhizogenes MSU440 or C58C1 was the most suitable for hairy root transformation. The transformation efficiency was highest when tissues contained hypocotyls as explants. The optimal procedure involves A. rhizogenes at an optical density (OD600) of 0.7 for 10 min and co-cultivation duration for four days, achieving a transgenic efficiency of up to 71.43%. An auxin responsive promoter DR5 ver2 carrying an enhanced green fluorescent protein (eGFP) marker was transformed by A. rhizogenes MSU440, thereby generating transgenic hairy roots capable of high eGFP expression in root tip and meristem of strawberry where auxin accumulated. Finally, this system was applied for functional analysis using jGCaMP7c, which could sense calcium signals. A significant upsurge in eGFP expression in the transgenic hairy roots was displayed after adding calcium chloride. The results suggested that this approach was feasible for studying specific promoters and could be a tool to analyze gene functions in the roots of strawberries. Conclusion We established a rapid and efficient hairy root transformation in strawberry by optimizing parameters, which was adequate for promoter analysis and functional characterization of candidate genes in strawberry and other rosaceous plants.
    Keywords Agrobacterium rhizogenes ; Woodland strawberry ; Hairy root ; Rosaceae ; Plant culture ; SB1-1110 ; Biology (General) ; QH301-705.5
    Subject code 580
    Language English
    Publishing date 2023-09-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Human milk oligosaccharide lacto-N-tetraose: Physiological functions and synthesis methods

    Hu, Miaomiao / Miao, Ming / Li, Kewen / Luan, Qingmin / Sun, Guilian / Zhang, Tao

    Carbohydrate Polymers. 2023 May 25, p.121067-

    2023  , Page(s) 121067–

    Abstract: Human milk oligosaccharides (HMOs) have attracted considerable attention due to their unique role in boosting infant health. Among the HMOs, lacto-N-tetraose (LNT) is a significant constituent associated with various health benefits, such as prebiotic ... ...

    Abstract Human milk oligosaccharides (HMOs) have attracted considerable attention due to their unique role in boosting infant health. Among the HMOs, lacto-N-tetraose (LNT) is a significant constituent associated with various health benefits, such as prebiotic effects, antiadhesive antimicrobials, antiviral protection, and immune modulators. LNT has received a “Generally Recognized as Safe” status by the American Food and Drug Administration and was approved as a food ingredient for infant formula. However, the limited availability of LNT poses a major challenge for its application in food and medicine. In this review, we first explored the physiological functions of LNT. Next, we describe several synthesis methods for production of LNT, including chemical, enzymatic, and cell factory approaches, and summarize the pivotal research results. Finally, challenges and opportunities for the large-scale synthesis of LNT were discussed.
    Keywords Food and Drug Administration ; anti-infective agents ; breast milk ; food composition ; infant formulas ; medicine ; oligosaccharides ; prebiotics ; Human milk oligosaccharides ; Lacto-N-tetraose ; Physiological functions ; Biosynthesis
    Language English
    Dates of publication 2023-0525
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2023.121067
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