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  1. Article: Eschar in a Belgian traveler returning from Central Europe.

    Costescu Strachinaru, Diana Isabela / Henckaerts, Liesbet / Depypere, Melissa / Vanbrabant, Peter

    Oxford medical case reports

    2024  Volume 2024, Issue 4, Page(s) omae032

    Abstract: Background: Tularemia is a zoonotic infection caused by : Patients and methods: A Belgian traveler returning from an adventurous vacation in Central Europe presents fever, flu-like symptoms, a skin ulcer with a necrotic center resembling an eschar on ...

    Abstract Background: Tularemia is a zoonotic infection caused by
    Patients and methods: A Belgian traveler returning from an adventurous vacation in Central Europe presents fever, flu-like symptoms, a skin ulcer with a necrotic center resembling an eschar on the left thigh and painful left inguinal lymphadenopathy. An enzyme linked immunosorbent assay developed by the National Reference Laboratory for Tularemia, Sciensano, Belgium, detected elevated Ig G antibodies against
    Results: A highly likely case of ulceroglandular tularemia is described and the differential diagnosis is discussed.
    Conclusion: The incidence of tularemia has been increasing throughout Europe in recent years. Physicians should be aware of this disease, its diversity of reservoirs, transmission routes and clinical presentations.
    Language English
    Publishing date 2024-04-25
    Publishing country England
    Document type Case Reports
    ZDB-ID 2766251-2
    ISSN 2053-8855
    ISSN 2053-8855
    DOI 10.1093/omcr/omae032
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  2. Article: The role of sonication in the diagnosis of periprosthetic joint infection in total shoulder arthroplasty.

    Mannaerts, Julie / Wijnen, Verena / Depypere, Melissa / Verhaegen, Filip / Debeer, Philippe

    Acta orthopaedica Belgica

    2023  Volume 89, Issue 2, Page(s) 201–206

    Abstract: An increased sensitivity of sonication compared to periprosthetic tissue cultures in the diagnosis of periprosthetic joint infection (PJI) of hip and knee arthroplasty has been reported. The goal of this study was to determine if there is also an added ... ...

    Abstract An increased sensitivity of sonication compared to periprosthetic tissue cultures in the diagnosis of periprosthetic joint infection (PJI) of hip and knee arthroplasty has been reported. The goal of this study was to determine if there is also an added value of implant sonication in the diagnosis of PJI in total shoulder arthroplasty (TSA). A retrospective analysis of patients who underwent removal of their TSA combined with sonication of the implant for suspicion of PJI between April 2009 and August 2017 was performed. The diagnosis of PJI was based on the major criteria described by Parvizi. We calculated sensitivity, specificity, predictive values, likelihood ratios and diagnostic accuracy for sonication cultures in comparison with periprosthetic tissue cultures. Data from 41 patients were analysed. Standard synovial fluid cultures combined with intraoperative periprosthetic tissue cultures had a sensitivity of 95%, specificity of 95% and total accuracy of 95%. Sonication cultures had a sensitivity of 91%, specificity of 68% and total accuracy of 80%. Six patients had negative standard cultures but positive sonication cultures. In patients with only one positive standard culture, the pathogen of the sonication culture corresponded to the pathogen of the positive soft tissue culture. We found a possible added value of sonication of TSA in the diagnosis of PJI in conjunction with standard intraoperative cultures. In some patients with suspicion of low-grade TSA infection, sonication could identify a possible causal microorganism despite negative standard cultures.
    MeSH term(s) Humans ; Arthroplasty, Replacement, Shoulder ; Sensitivity and Specificity ; Sonication ; Prosthesis-Related Infections/diagnosis ; Retrospective Studies ; Synovial Fluid ; Arthroplasty, Replacement, Hip
    Language English
    Publishing date 2023-11-04
    Publishing country Belgium
    Document type Journal Article
    ZDB-ID 210367-9
    ISSN 0001-6462 ; 1784-407X
    ISSN 0001-6462 ; 1784-407X
    DOI 10.52628/89.2.10875
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    Zs.A 37: Show issues Location:
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  3. Article ; Online: Laboratory workflow for optimizing leukocyte count and differential in synovial fluids on Sysmex XN-1000.

    Vanrenterghem, Marthe / Dom, Julie / Boeckx, Nancy / Depypere, Melissa / Frans, Glynis / Vles, Georges / Van Laer, Christine

    International journal of laboratory hematology

    2024  

    Abstract: Introduction: Falsely elevated synovial white blood cell (WBC) counts using automated hematology analyzers have been reported particularly in the setting of joint arthroplasty. We evaluated the implementation of a laboratory workflow based on Sysmex XN- ... ...

    Abstract Introduction: Falsely elevated synovial white blood cell (WBC) counts using automated hematology analyzers have been reported particularly in the setting of joint arthroplasty. We evaluated the implementation of a laboratory workflow based on Sysmex XN-1000-automated cell counting and scattergram interpretation.
    Methods: WBC and differential were measured for 76 synovial fluid samples (29 native joints and 47 with joint arthroplasties) with Sysmex XN-1000 and manual methods. All scattergrams were evaluated for possible incorrect WBC and/or differential according to our implemented workflow. A specific finding was the "banana-shape" scattergram, which indicates possible interferences. The European Bone & Joint Infection Society (EBJIS) criteria were applied to identify possible prosthetic joint infections (PJIs) in patients with joint arthroplasties.
    Results: Correlation between automated and manual WBC counts, calculated for samples with WBC count <50 000/μL, was higher for native joints (r = 0.938) compared with patients known with arthroplasty (r = 0.906). Scattergrams classified as OK showed overall a higher correlation compared with scattergrams, which were interpreted as NOT OK. "Banana-shape" scattergrams (n = 19) showed falsely elevated automated WBC count, and the patterns were mainly seen in prosthesis patients (17/19 [89%]). Six of 47 (13%) patients with joint arthroplasties were reclassified from "confirmed" to "unlikely" PJI according to the EBJIS criteria.
    Conclusion: Our workflow based on scattergram interpretation resulted in accurate WBC counts in synovial fluid using automated/and or manual methods. It is important to identify the presence of "banana-shape" scattergrams to avoid overestimated automated WBC counts. Overall, automated synovial WBC count can be used, even for patients with arthroplasty, but after visual inspection of the scattergram to exclude possible interferences.
    Language English
    Publishing date 2024-03-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2268590-X
    ISSN 1751-553X ; 1751-5521 ; 0141-9854
    ISSN (online) 1751-553X
    ISSN 1751-5521 ; 0141-9854
    DOI 10.1111/ijlh.14274
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    Zs.A 1499: Show issues Location:
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  4. Article ; Online: Comparison of the Euroimmun Borrelia 'antibody index' with Virotech immunoblot-based detection of intrathecal Borrelia antibody production for the diagnosis of Lyme neuroborreliosis.

    De Bont, Erika / Lagrou, Katrien / Depypere, Melissa

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

    2021  Volume 41, Issue 1, Page(s) 155–161

    Abstract: For diagnosis of neuroborreliosis, calculation of the antibody index, based on Euroimmun Anti-Borrelia plus VlsE ELISA was compared to Virotech Borrelia Europe plus TpN17 immunoblot-based detection of Borrelia-specific intrathecal antibody production. ... ...

    Abstract For diagnosis of neuroborreliosis, calculation of the antibody index, based on Euroimmun Anti-Borrelia plus VlsE ELISA was compared to Virotech Borrelia Europe plus TpN17 immunoblot-based detection of Borrelia-specific intrathecal antibody production. CXCL13 results in cerebrospinal fluid were used to evaluate discordant results. A total of 64 serum/CSF pairs were analysed. Patients were classified according to European Federation of Neurological Societies criteria incorporating Virotech results. For the Euroimmun assay, a sensitivity of 100% and specificity of 94% was found. Agreement between the both tests was almost perfect (κ 0.81). Both methods are appropriate for the detection of Borrelia-specific intrathecal antibody production.
    MeSH term(s) Adolescent ; Adult ; Aged ; Antibodies, Bacterial/analysis ; Antibodies, Bacterial/blood ; Borrelia/immunology ; Borrelia/isolation & purification ; Chemokine CXCL13/analysis ; Chemokine CXCL13/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Female ; Humans ; Immunoblotting/methods ; Lyme Neuroborreliosis/blood ; Lyme Neuroborreliosis/cerebrospinal fluid ; Lyme Neuroborreliosis/diagnosis ; Lyme Neuroborreliosis/microbiology ; Male ; Middle Aged ; Young Adult
    Chemical Substances Antibodies, Bacterial ; Chemokine CXCL13
    Language English
    Publishing date 2021-09-13
    Publishing country Germany
    Document type Comparative Study ; Journal Article
    ZDB-ID 603155-9
    ISSN 1435-4373 ; 0934-9723 ; 0722-2211
    ISSN (online) 1435-4373
    ISSN 0934-9723 ; 0722-2211
    DOI 10.1007/s10096-021-04343-x
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    Zs.A 1732: Show issues Location:
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  5. Article ; Online: Post-operative Outcomes for Vascular Rings: A Systematic Review and Meta-analysis.

    Rato, Joao / Zidere, Vita / François, Katrien / Boon, Mieke / Depypere, Anouk / Simpson, John M / Speggiorin, Simone / Vigneswaran, Trisha V

    Journal of pediatric surgery

    2023  Volume 58, Issue 9, Page(s) 1744–1753

    Abstract: Objectives: Vascular rings may cause tracheal and/or oesophageal compression. For many patients, symptoms/signs have been present for a long period before diagnosis. However, in the era of prenatal diagnosis, some units advocate universal early surgery. ...

    Abstract Objectives: Vascular rings may cause tracheal and/or oesophageal compression. For many patients, symptoms/signs have been present for a long period before diagnosis. However, in the era of prenatal diagnosis, some units advocate universal early surgery. The risks and efficacy of surgery must be known to adequately counsel for the operation. This meta-analysis sought to define the morbidity and mortality associated with surgical correction, and persistent post-operative symptoms.
    Methods: PubMed, Cochrane Library and CINAHL databases were searched for studies that described the outcome of patients undergoing surgery for a double or right aortic arch (DAA or RAA). Non-comparative and random effects model-based meta-analyses were conducted to calculate the pooled rates of mortality, surgical complications, reintervention, and persistent follow-up symptoms.
    Results: Nineteen eligible studies were included comprising 18 studies describing outcomes for DAA surgery and 15 for RAA surgery. For DAA surgery, overall mortality rate was 0% [95% confidence interval (CI) 0.0-1.0], post-surgical complication rate 18% [95% CI: 12.0-23.0], prevalence of reintervention 3% [95% CI: 1.0-5.0] and prevalence of symptoms at last follow-up was 33% [95% CI: 17.0-52.0]. For RAA surgery, overall pooled mortality was 0% [95% CI: 0.0-0.0], prevalence of post-surgical complications was 15% [95% CI: 8.0-23.0], reintervention rate was 2% [95% CI: 0.0-4.0], prevalence of symptoms at last follow-up was 40% [95% CI: 26.0-55.0].
    Conclusions: While surgery to correct a vascular ring is safe, the rate of persistent symptoms is high and further strategies must be sought to reduce this burden.
    MeSH term(s) Pregnancy ; Female ; Humans ; Vascular Ring ; Aorta, Thoracic/surgery ; Prenatal Diagnosis ; Trachea ; Subclavian Artery/surgery
    Language English
    Publishing date 2023-02-28
    Publishing country United States
    Document type Meta-Analysis ; Systematic Review ; Journal Article
    ZDB-ID 80165-3
    ISSN 1531-5037 ; 0022-3468
    ISSN (online) 1531-5037
    ISSN 0022-3468
    DOI 10.1016/j.jpedsurg.2023.02.058
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    Zs.A 607: Show issues Location:
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  6. Article ; Online: Screening for Congenital Cytomegalovirus Is Recommended in Every Newborn.

    Depypere, Melissa / Piérard, Denis

    Fetal diagnosis and therapy

    2017  Volume 44, Issue 1, Page(s) 79

    MeSH term(s) Cytomegalovirus ; Cytomegalovirus Infections ; Humans ; Infant, Newborn
    Language English
    Publishing date 2017-11-17
    Publishing country Switzerland
    Document type Journal Article ; Comment
    ZDB-ID 1066460-9
    ISSN 1421-9964 ; 1015-3837
    ISSN (online) 1421-9964
    ISSN 1015-3837
    DOI 10.1159/000481942
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  7. Article: Should treatment decisions in septic arthritis of the native hip joint be based on the route of infection?

    Ruythooren, Fred / Ghijselings, Stijn / Cools, Jordi / Depypere, Melissa / De Munter, Paul / Metsemakers, Willem-Jan / Vles, George

    Journal of bone and joint infection

    2023  Volume 8, Issue 5, Page(s) 209–218

    Abstract: ... ...

    Abstract Background
    Language English
    Publishing date 2023-10-12
    Publishing country Germany
    Document type Journal Article
    ISSN 2206-3552
    ISSN 2206-3552
    DOI 10.5194/jbji-8-209-2023
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  8. Article ; Online: The Microbiological Etiology of Fracture-Related Infection.

    Depypere, Melissa / Sliepen, Jonathan / Onsea, Jolien / Debaveye, Yves / Govaert, Geertje A M / IJpma, Frank F A / Zimmerli, Werner / Metsemakers, Willem-Jan

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 934485

    Abstract: Purpose: Fracture-related infection (FRI) is an important complication related to orthopaedic trauma. Although the scientific interest with respect to the diagnosis and treatment of FRI is increasing, data on the microbiological epidemiology remains ... ...

    Abstract Purpose: Fracture-related infection (FRI) is an important complication related to orthopaedic trauma. Although the scientific interest with respect to the diagnosis and treatment of FRI is increasing, data on the microbiological epidemiology remains limited. Therefore, the primary aim of this study was to evaluate the microbiological epidemiology related to FRI, including the association with clinical symptoms and antimicrobial susceptibility data. The secondary aim was to analyze whether there was a relationship between the time to onset of infection and the microbiological etiology of FRI.
    Methods: FRI patients treated at the University Hospitals of Leuven, Belgium, between January 1st 2015 and November 24th 2019 were evaluated retrospectively. The microbiological etiology and antimicrobial susceptibility data were analyzed. Patients were classified as having an early (<2 weeks after implantation), delayed (2-10 weeks) or late-onset (> 10 weeks) FRI.
    Results: One hundred ninety-one patients with 194 FRIs, most frequently involving the tibia (23.7%) and femur (18.6%), were included.
    Conclusion: This study revealed that in early FRIs, polymicrobial infections and infections including Enterobacterales and enterococcal species were more frequent. A time-based FRI classification is not meaningful to estimate the microbiological epidemiology and cannot be used to guide empiric antibiotic therapy. Large multicenter prospective studies are necessary to gain more insight into the added value of (broad) empirical antibiotic therapy.
    MeSH term(s) Anti-Bacterial Agents/therapeutic use ; Coinfection/drug therapy ; Gram-Negative Bacteria ; Humans ; Microbial Sensitivity Tests ; Prospective Studies ; Retrospective Studies ; Staphylococcus epidermidis
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2022-07-07
    Publishing country Switzerland
    Document type Journal Article ; Multicenter Study
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.934485
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  9. Article ; Online: Corrigendum to: Validation of the diagnostic criteria of the consensus definition of fracture-related infectionInjury (2022);53, pages 1867-1879.

    Onsea, Jolien / Van Lieshout, Esther M M / Zalavras, Charalampos / Sliepen, Jonathan / Depypere, Melissa / Noppe, Nathalie / Ferguson, Jamie / Verhofstad, Michael H J / Govaert, Geertje A M / IJpma, Frank F A / McNally, Martin A / Metsemakers, Willem-Jan

    Injury

    2023  

    Language English
    Publishing date 2023-03-21
    Publishing country Netherlands
    Document type Published Erratum
    ZDB-ID 218778-4
    ISSN 1879-0267 ; 0020-1383
    ISSN (online) 1879-0267
    ISSN 0020-1383
    DOI 10.1016/j.injury.2023.03.021
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    Zs.A 778: Show issues Location:
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  10. Article ; Online: No in vitro activity of cefiderocol against OXA-427-producing Enterobacterales.

    Jacob, Ann-Sophie / Chong, Ga-Lai / Lagrou, Katrien / Depypere, Melissa / Desmet, Stefanie

    The Journal of antimicrobial chemotherapy

    2021  Volume 76, Issue 12, Page(s) 3317–3318

    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Cephalosporins ; Microbial Sensitivity Tests ; beta-Lactamases/genetics ; Cefiderocol
    Chemical Substances Anti-Bacterial Agents ; Cephalosporins ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2021-08-24
    Publishing country England
    Document type Letter
    ZDB-ID 191709-2
    ISSN 1460-2091 ; 0305-7453
    ISSN (online) 1460-2091
    ISSN 0305-7453
    DOI 10.1093/jac/dkab304
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