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Article ; Online: A response to the commentary by Jianquan Yang, Wen Guo, Rong Huang, Chunyang Zhou, and Man Lu.

Zheng, Zhen-Yuan / Xu, Li-Yan

2022 Volume 533, Page(s) 215596

Language	English
Publishing date	2022-02-15
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	195674-7
ISSN	1872-7980 ; 0304-3835
ISSN (online)	1872-7980
ISSN	0304-3835
DOI	10.1016/j.canlet.2022.215596
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Article ; Online: Herba Cistanche (Rou Cong Rong): A Review of Its Phytochemistry and Pharmacology.

Lei, Huibo / Wang, Xinyu / Zhang, Yuhao / Cheng, Taofang / Mi, Rui / Xu, Xike / Zu, Xianpeng / Zhang, Weidong

Chemical & pharmaceutical bulletin

2020 Volume 68, Issue 8, Page(s) 694–712

Abstract: Herba Cistanche, known as Rou Cong Rong in Chinese, is a very valuable Chinese herbal medicine ... that has been recorded in the Chinese Pharmacopoeia. Rou Cong Rong has been extensively used in clinical ... Rong, the most important of which are phenylethanoid glycosides. This article summarizes the up-to-date ...

Abstract	Herba Cistanche, known as Rou Cong Rong in Chinese, is a very valuable Chinese herbal medicine that has been recorded in the Chinese Pharmacopoeia. Rou Cong Rong has been extensively used in clinical practice in traditional herbal formulations and has also been widely used as a health food supplement for a long time in Asian countries such as China and Japan. There are many bioactive compounds in Rou Cong Rong, the most important of which are phenylethanoid glycosides. This article summarizes the up-to-date information regarding the phytochemistry, pharmacology, processing, toxicity and safety of Rou Cong Rong to reveal its pharmacodynamic basis and potential therapeutic effects, which could be of great value for its use in future research.
MeSH term(s)	Animals ; Cistanche/chemistry ; Cistanche/metabolism ; Drugs, Chinese Herbal ; Gastrointestinal Microbiome/drug effects ; Glycosides/chemistry ; Glycosides/isolation & purification ; Glycosides/pharmacology ; Glycosides/therapeutic use ; Monoterpenes/chemistry ; Monoterpenes/isolation & purification ; Monoterpenes/pharmacology ; Monoterpenes/therapeutic use ; Oxidative Phosphorylation/drug effects ; Parkinson Disease/drug therapy ; Parkinson Disease/veterinary ; Phenylethyl Alcohol/chemistry ; Phytochemicals/chemistry ; Phytochemicals/pharmacology ; Phytochemicals/therapeutic use
Chemical Substances	Drugs, Chinese Herbal ; Glycosides ; Monoterpenes ; Phytochemicals ; Phenylethyl Alcohol (ML9LGA7468)
Language	English
Publishing date	2020-07-07
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	213307-6
ISSN	1347-5223 ; 0009-2363
ISSN (online)	1347-5223
ISSN	0009-2363
DOI	10.1248/cpb.c20-00057
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Zs.A 770: Show issues

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Article: Mechanisms of Cong Rong Shu Jing Compound Effects on Endoplasmic Reticulum Stress in a Rat Model of Parkinson's Disease.

Lin, Yao / Tang, Lanfang / Huang, Peizhen / Liu, Ting / Zhong, Jianan / Xu, Qian / Li, Xiyu / Lin, Youning / Xiao, Shaojian / Cai, Jing

Evidence-based complementary and alternative medicine : eCAM

2020 Volume 2020, Page(s) 1818307

Abstract: This study investigated the effects of the Cong Rong Shu Jing (CRSJ) compound ...

Abstract	This study investigated the effects of the Cong Rong Shu Jing (CRSJ) compound on endoplasmic reticulum stress in a rat model of Parkinson's disease (PD). A total of 40 rats were subcutaneously injected with rotenone-sunflower oil emulsion into the back of the neck to establish a rat model of PD. These PD rats were randomly divided into low-, medium-, and high-dose groups (intragastric administration of 0.5, 1, and 2 g/kg CRSJ, respectively) and a model group (intragastric administration of the solvent; 10 rats per group). Furthermore, 10 rats each were attributed to the control and vehicle groups (both received intragastric administration of the CRSJ solvent, and the vehicle group were injected additionally with sunflower oil alone). A traction test was conducted two times, after the PD model establishment and after 14 days of CRSJ gavage. The numbers of tyrosine hydroxylase- (TH-) positive cells and the dopamine levels in the substantia nigra were assessed using immunohistochemistry and high-performance liquid chromatography, respectively. Western blotting detected the expression levels of
Language	English
Publishing date	2020-05-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2020/1818307
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Zs.A 5995: Show issues

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Article ; Online: Detection of Cistanches Herba (Rou Cong Rong) Medicinal Products Using Species-Specific Nucleotide Signatures

Xiao-yue Wang / Rong Xu / Jun Chen / Jing-yuan Song / Steven-G Newmaster / Jian-ping Han / Zheng Zhang / Shi-lin Chen

Frontiers in Plant Science, Vol

2018 Volume 9

Abstract: Cistanches Herba is a medicinal plant that has tonification properties and is commonly used in Asia. Owing to the imbalance between supply and demand, adulterants are frequently added for profit. However, there is no regulatory oversight because quality ... ...

Abstract	Cistanches Herba is a medicinal plant that has tonification properties and is commonly used in Asia. Owing to the imbalance between supply and demand, adulterants are frequently added for profit. However, there is no regulatory oversight because quality control tools are not sufficient for identifying heavily processed products. Thus, a novel molecular tool based on nucleotide signatures and species-specific primers was developed. The ITS2 regions from 251 Cistanches Herba and adulterant samples were sequenced. On the basis of SNP sites, four nucleotide signatures within 30~37 bp and six species-specific primers were developed, and they were validated by artificial experimental mixtures consisting of six different species and different ratios. This method was also applied to detect 66 Cistanches Herba products on the market, including extracts and Chinese patent medicines. The results demonstrated the utility of nucleotide signatures in identifying adulterants in mixtures. The market study revealed 36.4% adulteration: 19.7% involved adulteration with Cynomorium songaricum or Cistanche sinensis, and 16.7% involved substitution with Cy. songaricum, Ci. sinensis, or Boschniakia rossica. The results also revealed that Cy. songaricum was the most common adulterant in the market. Thus, we recommend the use of species-specific nucleotide signatures for regulating adulteration and verifying the quality assurance of medicinal product supply chains, especially for processed products whose DNA is degraded.
Keywords	Cistanches Herba ; Chinese patent medicine ; nucleotide signature ; degraded DNA ; medicine quality control ; Plant culture ; SB1-1110
Subject code	590
Language	English
Publishing date	2018-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Mechanisms of Cong Rong Shu Jing Compound Effects on Endoplasmic Reticulum Stress in a Rat Model of Parkinson’s Disease

Yao Lin / Lanfang Tang / Peizhen Huang / Ting Liu / Jianan Zhong / Qian Xu / Xiyu Li / Youning Lin / Shaojian Xiao / Jing Cai

Evidence-Based Complementary and Alternative Medicine, Vol

2020 Volume 2020

Abstract: This study investigated the effects of the Cong Rong Shu Jing (CRSJ) compound ...

Abstract	This study investigated the effects of the Cong Rong Shu Jing (CRSJ) compound on endoplasmic reticulum stress in a rat model of Parkinson's disease (PD). A total of 40 rats were subcutaneously injected with rotenone-sunflower oil emulsion into the back of the neck to establish a rat model of PD. These PD rats were randomly divided into low-, medium-, and high-dose groups (intragastric administration of 0.5, 1, and 2 g/kg CRSJ, respectively) and a model group (intragastric administration of the solvent; 10 rats per group). Furthermore, 10 rats each were attributed to the control and vehicle groups (both received intragastric administration of the CRSJ solvent, and the vehicle group were injected additionally with sunflower oil alone). A traction test was conducted two times, after the PD model establishment and after 14 days of CRSJ gavage. The numbers of tyrosine hydroxylase- (TH-) positive cells and the dopamine levels in the substantia nigra were assessed using immunohistochemistry and high-performance liquid chromatography, respectively. Western blotting detected the expression levels of α-synuclein, endoplasmic reticulum stress pathways-related proteins, cerebral dopamine neurotrophic factor (CDNF), mesencephalic astrocyte-derived neurotrophic factor (MANF), and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway-related proteins. Compared with the model group, the number of TH-positive cells in the substantia nigra was increased in the CRSJ groups. The expression levels of α-synuclein and the endoplasmic reticulum stress pathways-associated proteins glucose regulatory protein 78, inositol-requiring enzyme 1, apoptosis signal-regulating kinase 1, phosphorylated c-Jun N-terminal kinase, and caspase-12 were reduced. However, CRSJ administration elevated the expression levels of the neurotrophic factors CDNF and MANF, as well as those of p-PI3K and p-AKT. The CRSJ compound can relieve endoplasmic reticulum stress in PD rats and exerts protective effects in this animal model. These effects may be ...
Keywords	Other systems of medicine ; RZ201-999
Subject code	630
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Unsuccessful attempt at gene-editing by homologous recombination in the zebrafish germ line using the approach of "Rong and Golic".

Brookfield, Rosalind / Dafhnis-Calas, Felix / Xu, Zhengyao / Brown, William

Transgenic research

2012 Volume 21, Issue 5, Page(s) 1125–1136

Abstract: ... by homologous recombination in zebrafish analogous to that developed by Rong and Golic (Rong and Golic in Genetics 157:1307 ...

Abstract	We have investigated the practicality of implementing a strategy for site-specific editing by homologous recombination in zebrafish analogous to that developed by Rong and Golic (Rong and Golic in Genetics 157:1307-1312, 2001) in Drosophila melanogaster. We analysed approximately 7,300 offspring from 22 crosses and demonstrated successful excision of the gene editing construct but failed to detect either gene editing or the random integration of the intact editing construct subsequent to excision. The clustering of events in our data set demonstrates that the excision events are not occurring independently and emphasise that a promoter driving high level, tissue-specific transcription in meiotic cells is likely to be necessary if this general approach to site-specific editing by homologous recombination is to fulfil its potential.
MeSH term(s)	Animals ; Animals, Genetically Modified/genetics ; Animals, Genetically Modified/metabolism ; Crosses, Genetic ; DNA Breaks, Double-Stranded ; Deoxyribonucleases/metabolism ; Embryo, Nonmammalian/cytology ; Embryo, Nonmammalian/metabolism ; Gene Targeting/methods ; Gene Transfer Techniques ; Genetic Loci ; Germ Cells/cytology ; Germ Cells/metabolism ; Green Fluorescent Proteins/metabolism ; Homologous Recombination ; Meiosis ; Plasmids/genetics ; Plasmids/metabolism ; Promoter Regions, Genetic ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Transcription, Genetic ; Transgenes ; Zebrafish/genetics ; Zebrafish/metabolism
Chemical Substances	Recombinant Fusion Proteins ; Green Fluorescent Proteins (147336-22-9) ; Deoxyribonucleases (EC 3.1.-)
Language	English
Publishing date	2012-03-21
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	31620-9
ISSN	1573-9368 ; 0962-8819
ISSN (online)	1573-9368
ISSN	0962-8819
DOI	10.1007/s11248-012-9607-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Unsuccessful attempt at gene-editing by homologous recombination in the zebrafish germ line using the approach of “Rong and Golic”

Brookfield, Rosalind / Dafhnis-Calas, Felix / Xu, Zhengyao / Brown, William

Transgenic research. 2012 Oct., v. 21, no. 5

2012

Abstract: ... by homologous recombination in zebrafish analogous to that developed by Rong and Golic (Rong and Golic in Genetics 157:1307 ...

Abstract	We have investigated the practicality of implementing a strategy for site-specific editing by homologous recombination in zebrafish analogous to that developed by Rong and Golic (Rong and Golic in Genetics 157:1307–1312, 2001) in Drosophila melanogaster. We analysed approximately 7,300 offspring from 22 crosses and demonstrated successful excision of the gene editing construct but failed to detect either gene editing or the random integration of the intact editing construct subsequent to excision. The clustering of events in our data set demonstrates that the excision events are not occurring independently and emphasise that a promoter driving high level, tissue-specific transcription in meiotic cells is likely to be necessary if this general approach to site-specific editing by homologous recombination is to fulfil its potential.
Keywords	Danio rerio ; crossing ; data collection ; genes ; germ cells ; homologous recombination ; progeny
Language	English
Dates of publication	2012-10
Size	p. 1125-1136.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	31620-9
ISSN	1573-9368 ; 0962-8819
ISSN (online)	1573-9368
ISSN	0962-8819
DOI	10.1007/s11248-012-9607-1
Database	NAL-Catalogue (AGRICOLA)

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Book ; Online: Mechanical Properties of Advanced Multifunctional Coatings

Xu, Rong-Guang / Chen, Zhitong / Chen, Peijian / Peng, Guangjian

2023

Keywords	Technology: general issues ; History of engineering & technology ; Materials science ; ceramic matrix composite ; high temperature strength ; SiC fiber ; fiber-matrix interface coatings ; residual stress ; diamond-like carbon (DLC) ; plasma emission intensity ; magnetron sputtering ; tribological performance ; hot casting metals ; explosive compression bonding ; dovetail groove ; metallographic analysis ; mechanics analysis ; complex network theory ; sandstone ; network topology ; seepage ; distribution of degree ; structure of engineering coatings ; pore network model ; geometric distribution ; micropitting ; gear surface fatigue ; dedendum ; addendum ; SiO2 thin film ; nanoindentation ; surface morphology ; load-penetration curve ; hardness ; lubrication ; nanotribology ; phase transition ; adsorption ; Monte Carlo simulation ; molecular dynamics ; thermal barrier coatings ; thermal oxidation stress ; Cr3+ photoluminescence piezospectroscopy ; data processing method ; fitting function ; Lorentzian functions ; spectromechanics ; anti-reflection ; optical ; interference ; scratch ; damage ; CFD simulation ; ultraviolet nanoimprint lithography ; filling behavior ; moth-eye nanostructure ; thermal barrier coating ; terahertz time-domain spectroscopy (THz-TDS) ; stress optic coefficient ; barycenter method ; nanolaminates ; atomic layer deposition ; nanoengineering ; n/a
Language	English
Size	1 electronic resource (196 pages)
Publisher	MDPI - Multidisciplinary Digital Publishing Institute
Publishing place	Basel
Document type	Book ; Online
Note	English
HBZ-ID	HT030378395
ISBN	9783036566719 ; 3036566716
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Construction of Spiro[benzo[

Xu, Linlin / Rong, Yuchen / Liao, Hailin / Pan, Mei / Qian, Yuliang / Rong, Liangce / Zhang, Jinpeng

The Journal of organic chemistry

2023 Volume 88, Issue 11, Page(s) 6995–7005

Abstract: An efficient three-component reaction to access spiro[benzo[ ...

Abstract	An efficient three-component reaction to access spiro[benzo[
Language	English
Publishing date	2023-05-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	123490-0
ISSN	1520-6904 ; 0022-3263
ISSN (online)	1520-6904
ISSN	0022-3263
DOI	10.1021/acs.joc.3c00304
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Article ; Online: Biodegradation of low-density polyethylene film by two bacteria isolated from plastic debris in coastal beach.

Rong, Zhen / Xu, Xue-Wei / Wu, Yue-Hong

Ecotoxicology and environmental safety

2024 Volume 278, Page(s) 116445

Abstract: Low-density polyethylene (LDPE) conduces massive environmental accumulation due to its high production and recalcitrance to environment. In this study, We successfully enriched and isolated two strains, Nitratireductor sp. Z-1 and Gordonia sp. Z-2, from ... ...

Abstract	Low-density polyethylene (LDPE) conduces massive environmental accumulation due to its high production and recalcitrance to environment. In this study, We successfully enriched and isolated two strains, Nitratireductor sp. Z-1 and Gordonia sp. Z-2, from coastal plastic debris capable of degrading LDPE film. After a 30-day incubation at 30 ℃, strains Z-1 and Z-2 decreased the weight of branched-LDPE (BLDPE) film by 2.59 % and 10.27 % respectively. Furthermore, high temperature gel permeation chromatography (HT-GPC) analysis revealed molecular weight reductions of 7.69 % (Z-1) and 23.22 % (Z-2) in the BLDPE film. Scanning electron microscope (SEM) image showed the presence of microbial colonization and perforations on the film's surface. Fourier transform infrared spectroscopy (FTIR) analysis indicated novel functional groups, such as carbonyl and carbon-carbon double bonds in LDPE films. During LDPE degradation, both strains produced extracellular reactive oxygen species (ROS). GC-MS analysis revealed the degradation products included short-chain alkanes, alkanols, fatty acids, and esters. Genomic analysis identified numerous extracellular enzymes potentially involved in LDPE chain scission. A model was proposed suggesting a coordinated role between ROS and extracellular enzymes in the biodegradation of LDPE. This indicates strains Z-1 and Z-2 can degrade LDPE, providing a basis for deeper exploration of biodegradation mechanisms.
Language	English
Publishing date	2024-05-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	436536-7
ISSN	1090-2414 ; 0147-6513
ISSN (online)	1090-2414
ISSN	0147-6513
DOI	10.1016/j.ecoenv.2024.116445
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