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Article ; Online: ENA-78 Is a Novel Predictor of Wound Healing in Patients with Diabetic Foot Ulcers.

Li, Ju-Yi / Wang, Zhong-Jing / Deng, Ai-Ping / Li, Yu-Ming

2019 Volume 2019, Page(s) 2695436

Abstract: ... respectively. In an independent cohort, quantitative ELISA validation confirmed a decrease in MCP-2 and ENA-78 ... hyperlipidemia, and antibiotic therapy), only wound exudate level of ENA-78 remained having a significant ... Conclusion: Decreased wound exudate ENA-78 was independently associated with wound healing of patients ...

Abstract	Objectives: Chronic foot ulceration is a severe complication of diabetes, driving morbidity and mortality. The aim of our study was to identify novel biomarkers of impaired wound healing in diabetic foot ulcers. Methods: 109 patients with neuropathic diabetic foot ulcers and 30 burn victims otherwise healthy participated. Antibody-coated glass slide arrays were used to determine the levels of 80 human cytokines in pooled plasma or pooled wound exudate of diabetic foot ulcers with rapidly healing (RH, Results: Protein array profiling identified 27 proteins or 15 proteins significantly altered in protein profiling of pooled plasma or pooled wound exudate of 12 RH patients compared with 12 matched NH patients, respectively. In an independent cohort, quantitative ELISA validation confirmed a decrease in MCP-2 and ENA-78 levels in NH patients versus RH patients or burn victims. After adjusting for the traditional risk factors (sex, age, body mass index, fasting plasma glucose, ulcer area, HbA1C, diabetes duration, hyperlipidemia, and antibiotic therapy), only wound exudate level of ENA-78 remained having a significant association with an increased odds ratio (OR) for wound healing by binary logistic regression analysis ( Conclusion: Decreased wound exudate ENA-78 was independently associated with wound healing of patients with diabetic foot. Exudate ENA-78 level is implicated as a novel predictor of wound healing in patients with diabetic foot ulcers.
MeSH term(s)	Aged ; Biomarkers/metabolism ; Chemokine CXCL5/blood ; Chemokine CXCL5/metabolism ; Diabetic Foot/blood ; Diabetic Foot/metabolism ; Exudates and Transudates/metabolism ; Female ; Humans ; Male ; Middle Aged ; Wound Healing/physiology
Chemical Substances	Biomarkers ; Chemokine CXCL5
Language	English
Publishing date	2019-01-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2711897-6
ISSN	2314-6753 ; 2314-6753
ISSN (online)	2314-6753
ISSN	2314-6753
DOI	10.1155/2019/2695436
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Book ; Online: Impact of dry intrusion events on the composition and mixing state of particles during the winter Aerosol and Cloud Experiment in the Eastern North Atlantic (ACE-ENA)

Tomlin, Jay M. / Jankowski, Kevin A. / Veghte, Daniel P. / China, Swarup / Wang, Peiwen / Fraund, Matthew / Weis, Johannes / Zheng, Guangjie / Wang, Yang / Rivera-Adorno, Felipe / Raveh-Rubin, Shira / Knopf, Daniel A. / Wang, Jian / Gilles, Mary K. / Moffet, Ryan C. / Laskin, Alexander

eISSN: 1680-7324

2021

Abstract: ... Aerosol and Cloud Experiment in the Eastern North Atlantic (ACE-ENA) campaign. Particles were collected ...

Abstract	Long-range transport of continental emissions has a far-reaching influence over remote regions, resulting in substantial change in the size, morphology, and composition of the local aerosol population and cloud condensation nuclei (CCN) budget. Here, we investigate the physicochemical properties of atmospheric particles collected on board a research aircraft flown over the Azores during the winter 2018 Aerosol and Cloud Experiment in the Eastern North Atlantic (ACE-ENA) campaign. Particles were collected within the marine boundary layer (MBL) and free troposphere (FT) after long-range atmospheric transport episodes facilitated by dry intrusion (DI) events. Chemical and physical properties of individual particles were investigated using complementary capabilities of computer-controlled scanning electron microscopy and X-ray spectromicroscopy to probe particle external and internal mixing state characteristics. Furthermore, real-time measurements of aerosol size distribution, cloud condensation nuclei (CCN) concentration, and back-trajectory calculations were utilized to help bring into context the findings from offline spectromicroscopy analysis. While carbonaceous particles were found to be the dominant particle type in the region, changes in the percent contribution of organics across the particle population (i.e., external mixing) shifted from 68 % to 43 % in the MBL and from 92 % to 46 % in FT samples during DI events. This change in carbonaceous contribution is counterbalanced by the increase in inorganics from 32 % to 57 % in the MBL and 8 % to 55 % in FT. The quantification of the organic volume fraction (OVF) of individual particles derived from X-ray spectromicroscopy, which relates to the multi-component internal composition of individual particles, showed a factor of 2.06 ± 0.16 and 1.11 ± 0.04 increase in the MBL and FT, respectively, among DI samples. We show that supplying particle OVF into the κ -Köhler equation can be used as a good approximation of field-measured in situ CCN concentrations. We also report changes in the κ values in the MBL from κ MBL, non-DI =0.48 to κ MBL, DI =0.41 , while changes in the FT result in κ FT, non-DI =0.36 to κ FT, DI =0.33 , which is consistent with enhancements in OVF followed by the DI episodes. Our observations suggest that entrainment of particles from long-range continental sources alters the mixing state population and CCN properties of aerosol in the region. The work presented here provides field observation data that can inform atmospheric models that simulate sources and particle composition in the eastern North Atlantic.
Subject code	551
Language	English
Publishing date	2021-12-14
Publishing country	de
Document type	Book ; Online
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Article ; Online: Impact of dry intrusion events on the composition and mixing state of particles during the winter Aerosol and Cloud Experiment in the Eastern North Atlantic (ACE-ENA)

J. M. Tomlin / K. A. Jankowski / D. P. Veghte / S. China / P. Wang / M. Fraund / J. Weis / G. Zheng / Y. Wang / F. Rivera-Adorno / S. Raveh-Rubin / D. A. Knopf / J. Wang / M. K. Gilles / R. C. Moffet / A. Laskin

Atmospheric Chemistry and Physics, Vol 21, Pp 18123-

2021 Volume 18146

Abstract: ... Aerosol and Cloud Experiment in the Eastern North Atlantic (ACE-ENA) campaign. Particles were collected ...

Abstract	Long-range transport of continental emissions has a far-reaching influence over remote regions, resulting in substantial change in the size, morphology, and composition of the local aerosol population and cloud condensation nuclei (CCN) budget. Here, we investigate the physicochemical properties of atmospheric particles collected on board a research aircraft flown over the Azores during the winter 2018 Aerosol and Cloud Experiment in the Eastern North Atlantic (ACE-ENA) campaign. Particles were collected within the marine boundary layer (MBL) and free troposphere (FT) after long-range atmospheric transport episodes facilitated by dry intrusion (DI) events. Chemical and physical properties of individual particles were investigated using complementary capabilities of computer-controlled scanning electron microscopy and X-ray spectromicroscopy to probe particle external and internal mixing state characteristics. Furthermore, real-time measurements of aerosol size distribution, cloud condensation nuclei (CCN) concentration, and back-trajectory calculations were utilized to help bring into context the findings from offline spectromicroscopy analysis. While carbonaceous particles were found to be the dominant particle type in the region, changes in the percent contribution of organics across the particle population (i.e., external mixing) shifted from 68 % to 43 % in the MBL and from 92 % to 46 % in FT samples during DI events. This change in carbonaceous contribution is counterbalanced by the increase in inorganics from 32 % to 57 % in the MBL and 8 % to 55 % in FT. The quantification of the organic volume fraction (OVF) of individual particles derived from X-ray spectromicroscopy, which relates to the multi-component internal composition of individual particles, showed a factor of 2.06 ± 0.16 and 1.11 ± 0.04 increase in the MBL and FT, respectively, among DI samples. We show that supplying particle OVF into the κ -Köhler equation can be used as a good approximation of field-measured in situ CCN concentrations. We also report changes in the κ values in the MBL from κ MBL, non-DI =0.48 to κ MBL, DI =0.41 , while changes in the FT result in κ FT, non-DI =0.36 to κ FT, DI =0.33 , which is consistent with enhancements in OVF followed by the DI episodes. Our observations suggest that entrainment of particles from long-range continental sources alters the mixing state population and CCN properties of aerosol in the region. The work presented here provides field observation data that can inform atmospheric models that simulate sources and particle composition in the eastern North Atlantic.
Keywords	Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	551
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Copernicus Publications
Document type	Article ; Online
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Article ; Online: Lamellipodin promotes invasive 3D cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE.

Carmona, G / Perera, U / Gillett, C / Naba, A / Law, A-L / Sharma, V P / Wang, J / Wyckoff, J / Balsamo, M / Mosis, F / De Piano, M / Monypenny, J / Woodman, N / McConnell, R E / Mouneimne, G / Van Hemelrijck, M / Cao, Y / Condeelis, J / Hynes, R O /

Gertler, F B / Krause, M

Oncogene

2016 Volume 35, Issue 39, Page(s) 5155–5169

Abstract: ... Ena/VASP proteins and the Scar/WAVE complex, which stimulates actin branching. In contrast ... Lamellipodin interaction with Scar/WAVE but not with Ena/VASP is required for random 2D cell migration ... to Lamellipodin: Abl-mediated Lamellipodin phosphorylation promotes its association with both Scar/WAVE and Ena ...

Abstract	Cancer invasion is a hallmark of metastasis. The mesenchymal mode of cancer cell invasion is mediated by elongated membrane protrusions driven by the assembly of branched F-actin networks. How deregulation of actin regulators promotes cancer cell invasion is still enigmatic. We report that increased expression and membrane localization of the actin regulator Lamellipodin correlate with reduced metastasis-free survival and poor prognosis in breast cancer patients. In agreement, we find that Lamellipodin depletion reduced lung metastasis in an orthotopic mouse breast cancer model. Invasive 3D cancer cell migration as well as invadopodia formation and matrix degradation was impaired upon Lamellipodin depletion. Mechanistically, we show that Lamellipodin promotes invasive 3D cancer cell migration via both actin-elongating Ena/VASP proteins and the Scar/WAVE complex, which stimulates actin branching. In contrast, Lamellipodin interaction with Scar/WAVE but not with Ena/VASP is required for random 2D cell migration. We identified a phosphorylation-dependent mechanism that regulates selective recruitment of these effectors to Lamellipodin: Abl-mediated Lamellipodin phosphorylation promotes its association with both Scar/WAVE and Ena/VASP, whereas Src-dependent phosphorylation enhances binding to Scar/WAVE but not to Ena/VASP. Through these selective, regulated interactions Lamellipodin mediates directional sensing of epidermal growth factor (EGF) gradients and invasive 3D migration of breast cancer cells. Our findings imply that increased Lamellipodin levels enhance Ena/VASP and Scar/WAVE activities at the plasma membrane to promote 3D invasion and metastasis.
MeSH term(s)	Actin Cytoskeleton/genetics ; Animals ; Carrier Proteins/genetics ; Cell Adhesion Molecules/genetics ; Cell Movement/genetics ; DNA-Binding Proteins/genetics ; Epidermal Growth Factor/genetics ; Humans ; Mammary Neoplasms, Animal/genetics ; Mammary Neoplasms, Animal/pathology ; Membrane Proteins/genetics ; Mice ; Neoplasm Invasiveness/genetics ; Phosphorylation ; Protein Interaction Maps/genetics ; Wiskott-Aldrich Syndrome Protein Family/genetics
Chemical Substances	Carrier Proteins ; Cell Adhesion Molecules ; DNA-Binding Proteins ; ENA-VASP proteins ; Membrane Proteins ; RAPH1 protein, human ; Wasf1 protein, mouse ; Wiskott-Aldrich Syndrome Protein Family ; Epidermal Growth Factor (62229-50-9)
Language	English
Publishing date	2016-03-21
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	639046-8
ISSN	1476-5594 ; 0950-9232
ISSN (online)	1476-5594
ISSN	0950-9232
DOI	10.1038/onc.2016.47
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Article ; Online: Staphylococcus via an interaction with the ELR+ CXC chemokine ENA-78 is associated with BOS.

Gregson, A L / Wang, X / Injean, P / Weigt, S S / Shino, M / Sayah, D / DerHovanessian, A / Lynch, J P / Ross, D J / Saggar, R / Ardehali, A / Li, G / Elashoff, R / Belperio, J A

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

2015 Volume 15, Issue 3, Page(s) 792–799

Abstract: Staphylococcus aureus is the most commonly isolated gram-positive bacterium after lung transplantation (LT) and has been associated with poor posttransplant outcomes, but its effect on bronchiolitis obliterans syndrome (BOS) and death in the context of ... ...

Abstract	Staphylococcus aureus is the most commonly isolated gram-positive bacterium after lung transplantation (LT) and has been associated with poor posttransplant outcomes, but its effect on bronchiolitis obliterans syndrome (BOS) and death in the context of the allograft inflammatory environment has not been studied. A three-state Cox semi-Markovian model was used to determine the influence of allograft S. aureus and the ELR+ CXC chemokines on the survival rates and cause-specific hazards for movement from lung transplant (State 1) to BOS (State 2), from transplant (State 1) to death (State 3), and from BOS (State 2) to death (State 3). Acute rejection, pseudomonas pneumonia, bronchoalveolar lavage fluid (BALF) CXCL5 and its interaction with S. aureus all increased the likelihood of transition from transplant to BOS. Transition to death from transplant was facilitated by pseudomonas infection and single lung transplant. Movement from BOS to death was affected by the interaction between aspergillus, pseudomonas and CXCL5, but not S. aureus. S. aureus isolation had state specific effects after LT and only in concert with elevated BALF CXCL5 concentrations did it augment the risk of BOS. Pseudomonas and elevated BALF concentrations of CXCL5 continued as significant risk factors for BOS and death after BOS in lung transplantation.
MeSH term(s)	Bronchiolitis Obliterans/microbiology ; Bronchiolitis Obliterans/surgery ; Bronchoalveolar Lavage Fluid ; Chemokine CXCL5/metabolism ; Chemokines, CXC/metabolism ; Humans ; Lung Transplantation ; Staphylococcus aureus/pathogenicity ; Treatment Outcome
Chemical Substances	Chemokine CXCL5 ; Chemokines, CXC
Language	English
Publishing date	2015-02-12
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2060594-8
ISSN	1600-6143 ; 1600-6135
ISSN (online)	1600-6143
ISSN	1600-6135
DOI	10.1111/ajt.13029
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Zs.A 5542: Show issues

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Article ; Online: Translational medicine from observation to hypothesis to interpretation

Wang Ena

Journal of Translational Medicine, Vol 10, Iss Suppl 2, p A

2012 Volume 44

Keywords	Medicine ; R
Language	English
Publishing date	2012-10-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
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Article: The MAPK hypothesis: immune-regulatory effects of MAPK-pathway genetic dysregulations and implications for breast cancer immunotherapy.

Bedognetti, Davide / Roelands, Jessica / Decock, Julie / Wang, Ena / Hendrickx, Wouter

Emerging topics in life sciences

2021 Volume 1, Issue 5, Page(s) 429–445

Abstract: With the advent of checkpoint inhibition, immunotherapy has revolutionized the clinical management of several cancers, but has demonstrated limited efficacy in mammary carcinoma. Transcriptomic profiling of cancer samples defined distinct ... ...

Abstract	With the advent of checkpoint inhibition, immunotherapy has revolutionized the clinical management of several cancers, but has demonstrated limited efficacy in mammary carcinoma. Transcriptomic profiling of cancer samples defined distinct immunophenotypic categories characterized by different prognostic and predictive connotations. In breast cancer, genomic alterations leading to the dysregulation of mitogen-activated protein kinase (MAPK) pathways have been linked to an immune-silent phenotype associated with poor outcome and treatment resistance. These aberrations include mutations of MAP3K1 and MAP2K4, amplification of KRAS, BRAF, and RAF1, and truncations of NF1. Anticancer therapies targeting MAPK signaling by BRAF and MEK inhibitors have demonstrated clear immunologic effects. These off-target properties could be exploited to convert the immune-silent tumor phenotype into an immune-active one. Preclinical evidence supports that MAPK-pathway inhibition can dramatically increase the efficacy of immunotherapy. In this review, we provide a detailed overview of the immunomodulatory impact of MAPK-pathway blockade through BRAF and MEK inhibitions. While BRAF inhibition might be relevant in melanoma only, MEK inhibition is potentially applicable to a wide range of tumors. Context-dependent similarities and differences of MAPK modulation will be dissected, in light of the complexity of the MAPK pathways. Therapeutic strategies combining the favorable effects of MAPK-oriented interventions on the tumor microenvironment while maintaining T-cell function will be presented. Finally, we will discuss recent studies highlighting the rationale for the implementation of MAPK-interference approaches in combination with checkpoint inhibitors and immune agonists in breast cancer.
Language	English
Publishing date	2021-02-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2882721-1
ISSN	2397-8554 ; 2397-8554 ; 2397-8562
ISSN (online)	2397-8554
ISSN	2397-8554 ; 2397-8562
DOI	10.1042/ETLS20170142
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Article ; Online: An Octacarboxylate-Linked Sodium Metal-Organic Framework with High Porosity.

Miao, Jiafeng / Graham, Wells / Liu, Jiaqi / Hill, Ena Clementine / Ma, Lu-Lu / Ullah, Saif / Xia, Hai-Lun / Guo, Fu-An / Thonhauser, Timo / Proserpio, Davide M / Li, Jing / Wang, Hao

Journal of the American Chemical Society

2023 Volume 146, Issue 1, Page(s) 84–88

Abstract: Alkali metal-based metal-organic frameworks (MOFs) with permanent porosity are scarce because of their high tendency to coordinate with solvents such as water. However, these MOFs are lightweight and bear gravimetric benefits for gas adsorption related ... ...

Abstract	Alkali metal-based metal-organic frameworks (MOFs) with permanent porosity are scarce because of their high tendency to coordinate with solvents such as water. However, these MOFs are lightweight and bear gravimetric benefits for gas adsorption related applications. In this study, we present the successful construction of a microporous MOF, designated as HIAM-111, built solely on sodium ions by using an octacarboxylate linker. The structure of HIAM-111 is based on 8-connected Na
Language	English
Publishing date	2023-12-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.3c11260
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Article ; Online: Functional Genome Profiling to Understand Cancer Immune Responsiveness.

Wang, Ena / Bedognetti, Davide / Marincola, Francesco M

Methods in molecular biology (Clifton, N.J.)

2019 Volume 2055, Page(s) 231–244

Abstract: It has been almost two decades since we first proposed the use of minimally invasive serial biopsies to dissect the biology underlining cancer immune responsiveness (CIR) by looking for predictors of response, understanding mechanisms of action (MOA) of ... ...

Abstract	It has been almost two decades since we first proposed the use of minimally invasive serial biopsies to dissect the biology underlining cancer immune responsiveness (CIR) by looking for predictors of response, understanding mechanisms of action (MOA) of therapeutics and documenting strategies adopted by tumor cells to escape immune recognition. This approach led to the first description in 2002 of predictors of CIR, the characterization of the pharmacodynamics of several immune therapeutics, and the geneses of immune escape under immunological pressure prompted by successful treatment. The presumption was straightforward; study CIR where it occurs: the target organ. Since then, a large number of studies corroborated these early observations adding sophistication and accuracy to the investigations. Here, we summarize the history of functional genomic profiling as a discovery and validation tool for immune oncology (IO) and new insights that could be derived by single novel technologies.
MeSH term(s)	Biomarkers, Tumor/genetics ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Gene Regulatory Networks ; Genomics/methods ; Humans ; Immunotherapy/methods ; Mutation ; Neoplasms/drug therapy ; Neoplasms/genetics ; Treatment Outcome ; Tumor Escape
Chemical Substances	Biomarkers, Tumor
Language	English
Publishing date	2019-09-09
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-9773-2_11
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Article ; Online: MicroRNA Expression Profile Distinguishes Glioblastoma Stem Cells from Differentiated Tumor Cells

Sara Tomei / Andrea Volontè / Shilpa Ravindran / Stefania Mazzoleni / Ena Wang / Rossella Galli / Cristina Maccalli

Journal of Personalized Medicine, Vol 11, Iss 264, p

2021 Volume 264

Abstract: Glioblastoma (GBM) represents the most common and aggressive tumor of the brain. Despite the fact that several studies have recently addressed the molecular mechanisms underlying the disease, its etiology and pathogenesis are still poorly understood. GBM ...

Abstract	Glioblastoma (GBM) represents the most common and aggressive tumor of the brain. Despite the fact that several studies have recently addressed the molecular mechanisms underlying the disease, its etiology and pathogenesis are still poorly understood. GBM displays poor prognosis and its resistance to common therapeutic approaches makes it a highly recurrent tumor. Several studies have identified a subpopulation of tumor cells, known as GBM cancer stem cells (CSCs) characterized by the ability of self-renewal, tumor initiation and propagation. GBM CSCs have been shown to survive GBM chemotherapy and radiotherapy. Thus, targeting CSCs represents a promising approach to treat GBM. Recent evidence has shown that GBM is characterized by a dysregulated expression of microRNA (miRNAs). In this study we have investigated the difference between human GBM CSCs and their paired autologous differentiated tumor cells. Array-based profiling and quantitative Real-Time PCR (qRT-PCR) were performed to identify miRNAs differentially expressed in CSCs. The Cancer Genome Atlas (TCGA) data were also interrogated, and functional interpretation analysis was performed. We have identified 14 miRNAs significantly differentially expressed in GBM CSCs ( p < 0.005). MiR-21 and miR-95 were among the most significantly deregulated miRNAs, and their expression was also associated to patient survival. We believe that the data provided here carry important implications for future studies aiming at elucidating the molecular mechanisms underlying GBM.
Keywords	glioblastoma ; microRNAs ; cancer ; qPCR ; cancer stem cells ; Medicine ; R
Subject code	610
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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