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Article ; Online: A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells

Xisheng Li / Zhangjing Ma / Kathy O. Lui

STAR Protocols, Vol 4, Iss 3, Pp 102407- (2023)

2023

Abstract: Summary: By virtue of their capability to replicate and regenerate, human stem-cell-derived beta-like cells could be a valuable resource for cellular therapy targeting insulin-dependent diabetes. Here, we present a protocol to generate beta-like cells ... ...

Abstract	Summary: By virtue of their capability to replicate and regenerate, human stem-cell-derived beta-like cells could be a valuable resource for cellular therapy targeting insulin-dependent diabetes. Here, we present a protocol to generate beta-like cells from human embryonic stem cells (hESCs). We first describe steps for differentiation of beta-like cells from hESCs and CD9-negative beta-like cell enrichment through fluorescence-activated cell sorting. We then detail immunofluorescence, flow cytometry, and glucose-stimulated insulin secretion assay for characterization of human beta-like cells.For complete details on the use and execution of this protocol, please refer to Li et al. (2020).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Keywords	Cell Biology ; Stem Cells ; Science (General) ; Q1-390
Language	English
Publishing date	2023-09-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells.

Li, Xisheng / Ma, Zhangjing / Lui, Kathy O

STAR protocols

2023 Volume 4, Issue 3, Page(s) 102407

Abstract: By virtue of their capability to replicate and regenerate, human stem-cell-derived beta-like cells could be a valuable resource for cellular therapy targeting insulin-dependent diabetes. Here, we present a protocol to generate beta-like cells from human ... ...

Abstract	By virtue of their capability to replicate and regenerate, human stem-cell-derived beta-like cells could be a valuable resource for cellular therapy targeting insulin-dependent diabetes. Here, we present a protocol to generate beta-like cells from human embryonic stem cells (hESCs). We first describe steps for differentiation of beta-like cells from hESCs and CD9-negative beta-like cell enrichment through fluorescence-activated cell sorting. We then detail immunofluorescence, flow cytometry, and glucose-stimulated insulin secretion assay for characterization of human beta-like cells. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).
MeSH term(s)	Humans ; Human Embryonic Stem Cells ; Embryonic Stem Cells ; Insulin-Secreting Cells ; Cell Differentiation ; Pancreas ; Pancreatic Hormones
Chemical Substances	Pancreatic Hormones
Language	English
Publishing date	2023-06-30
Publishing country	United States
Document type	Journal Article
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2023.102407
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Article ; Online: The role of m6A mRNA modification in normal and malignant hematopoiesis.

Ma, Zhangjing / Sugimura, Rio / Lui, Kathy O

Journal of leukocyte biology

2023 Volume 115, Issue 1, Page(s) 100–115

Abstract: Hematopoiesis is a highly orchestrated biological process sustaining the supply of leukocytes involved in the maintenance of immunity, O2 and CO2 exchange, and wound healing throughout the lifetime of an animal, including humans. During early ... ...

Abstract	Hematopoiesis is a highly orchestrated biological process sustaining the supply of leukocytes involved in the maintenance of immunity, O2 and CO2 exchange, and wound healing throughout the lifetime of an animal, including humans. During early hematopoietic cell development, several waves of hematopoiesis require the precise regulation of hematopoietic ontogeny as well as the maintenance of hematopoietic stem and progenitor cells in the hematopoietic tissues, such as the fetal liver and bone marrow. Recently, emerging evidence has suggested the critical role of m6A messenger RNA (mRNA) modification, an epigenetic modification dynamically regulated by its effector proteins, in the generation and maintenance of hematopoietic cells during embryogenesis. In the adulthood, m6A has also been demonstrated to be involved in the functional maintenance of hematopoietic stem and progenitor cells in the bone marrow and umbilical cord blood, as well as the progression of malignant hematopoiesis. In this review, we focus on recent progress in identifying the biological functions of m6A mRNA modification, its regulators, and downstream gene targets during normal and pathological hematopoiesis. We propose that targeting m6A mRNA modification could offer novel insights into therapeutic development against abnormal and malignant hematopoietic cell development in the future.
MeSH term(s)	Animals ; Humans ; Adult ; Hematopoietic Stem Cells/metabolism ; Hematopoiesis/genetics ; Bone Marrow/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
Chemical Substances	6-methyladenine (W7IBY2BGAX) ; RNA, Messenger
Language	English
Publishing date	2023-05-16
Publishing country	England
Document type	Review ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1093/jleuko/qiad061
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Article ; Online: SARS-CoV-2 induced vascular endothelial dysfunction: direct or indirect effects?

Lui, Kathy O / Ma, Zhangjing / Dimmeler, Stefanie

Cardiovascular research

2023 Volume 120, Issue 1, Page(s) 34–43

Abstract: Clinical evidence reveals that manifestations of endothelial dysfunction are widely observed in COVID-19 and long-COVID patients. However, whether these detrimental effects are caused by direct infection of the endothelium or are indirectly mediated by ... ...

Abstract	Clinical evidence reveals that manifestations of endothelial dysfunction are widely observed in COVID-19 and long-COVID patients. However, whether these detrimental effects are caused by direct infection of the endothelium or are indirectly mediated by systemic inflammation has been a matter of debate. It has been well acknowledged that endothelial cells (ECs) of the cardiovascular system ubiquitously express the SARS-CoV-2 entry receptor angiotensin-converting enzyme 2 (ACE2), yet accumulating evidence suggests that it is more predominantly expressed by pericytes and vascular smooth muscle cells of the mammalian blood vessel. Besides, replicative infection of ECs by SARS-CoV-2 has yet to be demonstrated both in vitro and in vivo. In this study, we review latest research on endothelial ACE2 expression in different vascular beds, and the heterogeneity in various EC subsets with differential ACE2 expression in response to SARS-CoV-2. We also discuss ACE2-independent alternative mechanisms underlying endothelial activation in COVID-19, and the clinical manifestations of SARS-CoV-2-induced endothelial dysfunction. Altogether, understanding ACE2-dependent and ACE2-independent mechanisms driving SARS-CoV-2-induced vascular dysfunction would shed light on strategies of more effective therapies targeting cardiovascular complications associated with COVID-19.
MeSH term(s)	Animals ; Humans ; SARS-CoV-2/metabolism ; COVID-19 ; Angiotensin-Converting Enzyme 2/metabolism ; Endothelial Cells/metabolism ; Post-Acute COVID-19 Syndrome ; Peptidyl-Dipeptidase A/metabolism ; Vascular Diseases ; Mammals/metabolism
Chemical Substances	Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) ; Peptidyl-Dipeptidase A (EC 3.4.15.1)
Language	English
Publishing date	2023-12-29
Publishing country	England
Document type	Review ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80340-6
ISSN	1755-3245 ; 0008-6363
ISSN (online)	1755-3245
ISSN	0008-6363
DOI	10.1093/cvr/cvad191
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Article: Correction: Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis.

Ma, Zhangjing / Shi, Huiyuan / Shen, Yi / Li, Huixia / Yang, Yu / Yang, Jiange / Zhao, Hui / Wang, Gang / Wang, Jingqiang

Cell & bioscience

2023 Volume 13, Issue 1, Page(s) 54

Language	English
Publishing date	2023-03-11
Publishing country	England
Document type	Published Erratum
ZDB-ID	2593367-X
ISSN	2045-3701
ISSN	2045-3701
DOI	10.1186/s13578-023-01001-x
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Article ; Online: Integrated transcriptomic analysis on chicken ovary reveals CYP21A1 affects follicle granulosa cell development and steroid hormone synthesis.

You, Zhangjing / Yuan, Jingwei / Wang, Yuanmei / Sun, Yanyan / Ni, Aixin / Li, Yunlei / Ma, Hui / Ma, Tenghe / Chen, Jilan

Poultry science

2024 Volume 103, Issue 5, Page(s) 103589

Abstract: Egg production is an economically important trait in poultry breeding and production. Follicular development was regulated by several hormones released and genes expressed in the granulosa cells, impacting the egg production and fecundity of hens. ... ...

Abstract	Egg production is an economically important trait in poultry breeding and production. Follicular development was regulated by several hormones released and genes expressed in the granulosa cells, impacting the egg production and fecundity of hens. However, the molecular functions of these candidate genes that modulate these processes remain largely unknown. In the present study, bioinformatics analyses were performed to identify the candidate genes related to egg production in the ovarian tissue of White Leghorns with high egg production and Beijing You chicken with low egg production during sexual maturity and peak laying periods. The ovarian granulosa cells were used to assess the function of CYP21A1 by transfecting with CYP21A1-specific small interfering RNAs (siRNAs) and overexpression plasmids. We identified 514 differentially expressed genes (\|Log2(fold change) \| >1, P <0.05) between the 2 chicken breeds in both laying periods. Among these genes, CYP21A1, which is involved in the steroid hormone biosynthesis pathway was consistently upregulated in White Leghorns. Weighted gene co-expression network analysis (WGCNA) further suggested that CYP21A1 was a hub gene, which could positively respond to treatment with follicle stimulation hormone (FSH), affecting egg production. The interference of CYP21A1 significantly inhibited cell proliferation and promoted cell apoptosis. Overexpression of CYP21A1 promotes cell proliferation and inhibits cell apoptosis. Furthermore, the interference with CYP21A1 significantly downregulated the expression of STAR, CYP11A1, HSD3B1, and FSHR and also decreased the synthesis of progesterone (P4) and estradiol (E2) in granulosa cells. Overexpression of CYP21A1 increased the synthesis of P4 and estradiol E2 and the expression of steroid hormone synthesis-related genes in granulosa cells. Our findings provide new evidence for the biological role of CYP21A1 on granulosa cell proliferation, apoptosis, and steroid hormone synthesis, which lays the theoretical basis for improving egg production.
MeSH term(s)	Animals ; Female ; Chickens/genetics ; Chickens/physiology ; Granulosa Cells/metabolism ; Granulosa Cells/physiology ; Gene Expression Profiling/veterinary ; Avian Proteins/genetics ; Avian Proteins/metabolism ; Ovary/metabolism ; Gonadal Steroid Hormones/biosynthesis ; Gonadal Steroid Hormones/metabolism ; Transcriptome ; Ovarian Follicle/metabolism ; Ovarian Follicle/physiology
Chemical Substances	Avian Proteins ; Gonadal Steroid Hormones
Language	English
Publishing date	2024-02-24
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2024.103589
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Article: Vacuum Steam Treatment of

Juzwik, Jennifer / Hughes, Marc A / Chen, Zhangjing / Yang, Anna / Keith, Lisa M / White, Marshall S

Plant disease

2022 Volume 106, Issue 4, Page(s) 1114–1121

Abstract: A new and devastating disease, rapid ohia death (ROD), in Hawaii led to a state quarantine that regulates interisland transport of ohia wood and plant material to prevent spread of the causal pathogens. Heat treatments of ohia logs in commercial trade ... ...

Abstract	A new and devastating disease, rapid ohia death (ROD), in Hawaii led to a state quarantine that regulates interisland transport of ohia wood and plant material to prevent spread of the causal pathogens. Heat treatments of ohia logs in commercial trade were considered for phytosanitary treatment. Vacuum steam (VS) was evaluated for its ability to eradicate the pathogens,
MeSH term(s)	Ceratocystis ; Myrtaceae ; Steam ; Vacuum
Chemical Substances	Steam
Language	English
Publishing date	2022-03-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-07-21-1424-RE
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Article ; Online: Single-cell transcriptomics of Treg reveals hallmarks and trajectories of immunological aging.

Yang, Kevin Y / Liao, Jinyue / Ma, Zhangjing / Tse, Hung Fat / Lu, Liwei / Graca, Luis / Lui, Kathy O

Journal of leukocyte biology

2023 Volume 115, Issue 1, Page(s) 19–35

Abstract: Age-related immunosenescence is characterized by progressive dysfunction of adaptive immune response and increased autoimmunity. Nevertheless, the impact of aging on CD4+ regulatory T cells that are master regulators of the immune system remains largely ... ...

Abstract	Age-related immunosenescence is characterized by progressive dysfunction of adaptive immune response and increased autoimmunity. Nevertheless, the impact of aging on CD4+ regulatory T cells that are master regulators of the immune system remains largely unclear. Here, we report cellular and molecular hallmarks of regulatory T cells derived from murine lymphoid and adipose tissues at 3, 18, and 24 mo of age, respectively, by analyzing their heterogeneity that displays dynamic changes in transcriptomic effector signatures at a single-cell resolution. Although the proportion of regulatory T cells among total Cd4+ T cells, as well as their expression levels of Foxp3, did not show any global change with time, we have identified 6 transcriptomically distinct clusters of regulatory T cells with cross-tissue conserved hallmarks of aging, including increased numbers of proinflammatory regulatory T cells, reduced precursor cells, increased immature and mature T follicular regulatory cells potentially supported by a metabolic switch from oxidative phosphorylation to glycolysis, a gradual loss of CD150hi regulatory T cells that support hematopoiesis, and increased adipose tissue-specific regulatory T cells that are associated with metabolic disease. To dissect the impact of immunosenescence on humoral immunity, we propose some potential mechanisms underlying T follicular regulatory cell-mediated dysfunction by interactome analysis on T follicular regulatory cells, T follicular helper cells, and B cells during aging. Lastly, spatiotemporal analysis further revealed trajectories of regulatory T-cell aging that demonstrate the most significant changes in marrow and adipose tissues that might contribute to the development of age-related immunosenescence and type 2 diabetes. Taken together, our findings could provide a better understanding of age-associated regulatory T-cell heterogeneity in lymphoid and adipose tissues, as well as regulatory T-cell hallmarks during progressive adaptation to aging that could be therapeutically targeted for rejuvenating the aging immune system in the future.
MeSH term(s)	Mice ; Animals ; T-Lymphocytes, Regulatory ; T-Lymphocytes, Helper-Inducer ; Diabetes Mellitus, Type 2/metabolism ; Aging/genetics ; Gene Expression Profiling
Language	English
Publishing date	2023-09-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1093/jleuko/qiad104
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Article ; Online: Endothelial contribution to COVID-19: an update on mechanisms and therapeutic implications.

Ma, Zhangjing / Yang, Kevin Y / Huang, Yu / Lui, Kathy O

Journal of molecular and cellular cardiology

2021 Volume 164, Page(s) 69–82

Abstract: The global propagation of SARS-CoV-2 leads to an unprecedented public health emergency. Despite that the lungs are the primary organ targeted by COVID-19, systemic endothelial inflammation and dysfunction is observed particularly in patients with severe ... ...

Abstract	The global propagation of SARS-CoV-2 leads to an unprecedented public health emergency. Despite that the lungs are the primary organ targeted by COVID-19, systemic endothelial inflammation and dysfunction is observed particularly in patients with severe COVID-19, manifested by elevated endothelial injury markers, endotheliitis, and coagulopathy. Here, we review the clinical characteristics of COVID-19 associated endothelial dysfunction; and the likely pathological mechanisms underlying the disease including direct cell entry or indirect immune overreactions after SARS-CoV-2 infection. In addition, we discuss potential biomarkers that might indicate the disease severity, particularly related to the abnormal development of thrombosis that is a fatal vascular complication of severe COVID-19. Furthermore, we summarize clinical trials targeting the direct and indirect pathological pathways after SARS-CoV-2 infection to prevent or inhibit the virus induced endothelial disorders.
MeSH term(s)	Adolescent ; Adult ; Aged ; Angiotensin-Converting Enzyme 2/physiology ; Animals ; COVID-19/blood ; COVID-19/complications ; COVID-19/pathology ; COVID-19/physiopathology ; COVID-19/therapy ; Clinical Trials as Topic ; Endothelial Cells/pathology ; Endothelial Cells/virology ; Endothelium, Vascular/immunology ; Endothelium, Vascular/pathology ; Endothelium, Vascular/physiopathology ; HMGB1 Protein/physiology ; Humans ; Macaca mulatta ; Mice ; Neuropilin-1/physiology ; Oxidative Stress ; Reactive Oxygen Species ; Receptors, Virus/physiology ; SARS-CoV-2 ; Scavenger Receptors, Class B/physiology ; Severity of Illness Index ; Signal Transduction ; Systemic Inflammatory Response Syndrome/pathology ; Systemic Inflammatory Response Syndrome/physiopathology ; Thrombophilia/etiology ; Thrombophilia/physiopathology ; Vascular Endothelial Growth Factor A/physiology ; Vasculitis/etiology ; Vasculitis/immunology ; Vasculitis/physiopathology ; Young Adult
Chemical Substances	HMGB1 Protein ; HMGB1 protein, human ; NRP1 protein, human ; Reactive Oxygen Species ; Receptors, Virus ; SCARB1 protein, human ; Scavenger Receptors, Class B ; VEGFA protein, human ; Vascular Endothelial Growth Factor A ; Neuropilin-1 (144713-63-3) ; ACE2 protein, human (EC 3.4.17.23) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23)
Language	English
Publishing date	2021-11-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	80157-4
ISSN	1095-8584 ; 0022-2828
ISSN (online)	1095-8584
ISSN	0022-2828
DOI	10.1016/j.yjmcc.2021.11.010
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Article ; Online: Photo Responsive Electron and Proton Conductivity within a Hydrogen-Bonded Organic Framework.

Chen, Shimin / Ju, Yan / Zhang, Hao / Zou, Yingbing / Lin, Si / Li, Yunbin / Wang, Shuaiqi / Ma, En / Deng, Weihua / Xiang, Shengchang / Chen, Banglin / Zhang, Zhangjing

Angewandte Chemie (International ed. in English)

2023 Volume 62, Issue 34, Page(s) e202308418

Abstract: Rational design of crystalline porous materials with coupled proton-electron transfer has not yet been reported to date. Herein, we report a donor-acceptor (D-A) π-π stacking hydrogen-bonded organic framework (HOF; HOF-FJU-36) with zwitterionic 1,1'-bis( ... ...

Abstract	Rational design of crystalline porous materials with coupled proton-electron transfer has not yet been reported to date. Herein, we report a donor-acceptor (D-A) π-π stacking hydrogen-bonded organic framework (HOF; HOF-FJU-36) with zwitterionic 1,1'-bis(3-carboxybenzyl)-4,4'-bipyridinium (H
Language	English
Publishing date	2023-07-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.202308418
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